Distribution and Phenotypic Correlates of Variation at Enzyme Coding Loci in Rainbow Trout (Oncorhynchus mykiss) from the Lower Laurentian Great Lakes

1991 ◽  
Vol 48 (7) ◽  
pp. 1308-1315 ◽  
Author(s):  
Moira M. Ferguson ◽  
Peter E. Ihssen

The distribution of variation at 37 enzyme coding loci was determined in six samples of rainbow trout (Oncorhynchus mykiss) from hatchery and natural sources. Significant heterogeneity in allele frequencies was detected among samples of Lower Laurentian Great Lakes rainbow trout. Geographical proximity was a poor predictor of genetic similarity among samples. The adaptive significance of two loci was examined in 16 experimental families. Rainbow trout homozygous for an allele at a phosphoglucomutase structural gene (Pgm1-s), which results in no detectable activity of phosphoglucomutase (PGM1) in all tissues where this locus is normally expressed (e.g. muscle), were larger than those heterozygous or homozygous for the active allele. However, no significant differences in asymmetry of four bilateral meristic traits were detected between fish with different PGM1 phenotypes. Rainbow trout with PGM1 in liver because of a regulatory gene, Pgm1-t(b), were marginally larger than those without the allele. These data suggest a correlation between biochemical genetic variation and phenotypic characteristics associated with fitness.

1993 ◽  
Vol 71 (10) ◽  
pp. 1923-1933 ◽  
Author(s):  
Roy G. Danzmann ◽  
Moira M. Ferguson ◽  
Steven K. A. Arndt

We detected 142 six-base restriction sites and 30 haplotypes using 34 enzymes in a survey of mitochondrial DNA (mtDNA) variability in rainbow trout (Oncorhynchus mykiss) from various hatchery sources in Ontario and New York state, and one naturalized population in Lake Ontario. Five major phylogenetic groupings were identified, with the most divergent grouping (assemblage B) differing on average by 0.63% site divergence from other haplotypes; the two most divergent haplotypes differed by 15 site changes. All haplotypes detected in steelhead occur in the phylogenetic assemblage most closely related to chinook salmon (O. tshawytscha). The New York hatchery stocks have markedly lower mtDNA variability than the naturalized population from Ontario. Similarly, the Ontario and New York fish appear to have reduced mtDNA diversity relative to western fish. Significant heterogeneity in mtDNA haplotype frequencies was observed among most Ontario and New York populations examined. Some mtDNA haplotypes were unique to specific populations suggesting their utility as potential markers for genetic stock identification.


2005 ◽  
Vol 55 (4) ◽  
pp. 1521-1524 ◽  
Author(s):  
Isao Yumoto ◽  
Kikue Hirota ◽  
Yoshinobu Nodasaka ◽  
Kenji Nakajima

A halotolerant, obligately alkaliphilic bacterium, R-2T, was isolated from the skin of a rainbow trout (Oncorhynchus mykiss), a freshwater fish. The strain is Gram-positive, ferments several carbohydrates, is rod-shaped and motile by peritrichous flagella and produces ellipsoidal spores. The isolate grows at pH 9–10 but not at pH 7–8. This micro-organism grows in 0–22 % (w/v) NaCl at pH 10. Its major cellular fatty acids are iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, the major isoprenoid quinone is MK-7 and the DNA G+C content is 38·5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicates that strain R-2T is a member of the genus Oceanobacillus. DNA–DNA hybridization reveals low relatedness between the isolate and Oceanobacillus iheyensis (21·0 %). On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA relatedness data, the isolate should be designated as a novel species, for which the name Oceanobacillus oncorhynchi sp. nov. is proposed. The type strain is R-2T (=JCM 12661T=NCIMB 14022T).


Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Ghasemi Pirbalouti ◽  
E Pirali ◽  
G Pishkar ◽  
S Mohammadali Jalali ◽  
M Reyesi ◽  
...  

2014 ◽  
Author(s):  
Silvia Gonzalez-Rojo ◽  
Cristina Fernandez-Diez ◽  
Marta Lombo ◽  
Vanesa Robles Rodriguez ◽  
Herraez Maria Paz

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