Sublethal Effects of Petroleum Hydrocarbons on Adult American Lobsters (Homarus americanus)

1983 ◽  
Vol 40 (6) ◽  
pp. 705-717 ◽  
Author(s):  
Jerry F. Payne ◽  
J. Kiceniuk ◽  
R. Misra ◽  
Garth Fletcher ◽  
R. Thompson
Keyword(s):  
Amino Acid ◽  
Petroleum Hydrocarbons ◽  
Sublethal Effects ◽  
Freezing Point ◽  
Amino Nitrogen ◽  
Biological Response ◽  
Homarus Americanus ◽  
Point Sources ◽  
Monitoring Point ◽  
Oil Exposure

A suite of 56 different parameters including biochemical, physiological, and morphological indices were investigated in lobsters chronically exposed to a low level of petroleum hydrocarbons. General condition indices were unaffected and no oil-induced differences were noted in serum values for most of the electrolytes, except calcium, which was elevated in both sexes. Serum freezing-point depression decreased in both sexes of the experimental group. Considering the four major serum parameters, protein, lipid, glucose, and amino nitrogen, oil exposure had a significant effect on plasma protein and lipid values in the females. Chronic exposure to oil also had a marked impact on blood hemocytes in which lipid values were reduced by approximately one-half in both sexes. Three different types of oil-induced effects were noted for the essential amino acids, including (a) change in one sex only, (b) change in both sexes, and (c) interaction between sex and oil exposure. The only nonessential amino acid significantly affected by oil exposure was alanine. The differences observed in amino acid analogs, namely the elevation of serum levels of methionine sulfoxide and methylhistidine, have been discussed on the basis of mammalian studies as possible stress-related effects. One of the most interesting effects observed at the organismal level was gill browning, which markedly increased in the oil-exposed animals. This particular sublethal effect, possibly more than any other, could be considered pathological in nature. Gill browning is readily measurable and provides one suitable biological response for monitoring point sources of petroleum pollution in lobster habitats.

Osmoregulation in the Lobster Homarus americanus

1970 ◽  
Vol 27 (6) ◽  
pp. 1123-1130 ◽  
Author(s):  
W. Dall
Keyword(s):  
Freezing Point ◽  
Homarus Americanus ◽  
Salinity Range ◽  
Limited Extent ◽  
Salt Loading ◽  
Excess Water ◽  
Salt Excretion ◽  
Rapid Adjustment ◽  
Antennal Glands

Lobsters took 72 hr to adapt to a salinity of 20‰, with urine becoming markedly hypoosmotic to the blood during the first 25 hr, then increasing in concentration over the next 48 hr, though remaining significantly hypoosmotic to the blood. Adaptation to a salinity of 37‰ took 24 hr: the urine became almost isosmotic with the blood: the gastric and rectal fluids became hyperosmotic to the blood. Blood freezing-point determinations showed that over the salinity range 20–37‰ the lobster is able to osmoregulate to a limited extent only towards the lower end of the range, being otherwise an "osmocon-former." Salt-loading experiments indicated that the excess salts were rapidly excreted into the gut. It is concluded that the antennal glands are at least partly responsible for elimination of excess water, but that the gut is the site of salt excretion, and that there is rapid adjustment of salt imbalance.

MATHEMATICAL MODELS FOR THE SYNTHESIS OF PLANT-BASED COMPOSITIONS WITH IMPROVED AMINO ACID COMPOSITION

2021 ◽  
Author(s):  
Irina Gaivoronskaya ◽  
Valenitna Kolpakova
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Mathematical Models ◽  
Functional Properties ◽  
Amino Nitrogen ◽  
Essential Amino Acids ◽  
Major Allergen ◽  
Structural Features ◽  
Covalent Bonds ◽  
Pea Protein

The aim of the work was to optimize the process of obtaining multicomponent protein compositions with high biological value and higher functional properties than the original vegetable protein products. Was realized studies to obtain biocomposites on the base of pea protein-oat protein and pea protein-rice protein. Developed composites were enriched with all limited amino acids. For each of the essential amino acids, the amino acid score was 100% and higher. Protein products used in these compositions are not in major allergen list, which allows to use these compositions in allergen-free products and specialized nutrition. To determine biosynthesis parameters for compositions from pea protein and various protein concentrates with the use of transglutaminase enzyme, was studied effect of concentration and exposition time on the amount of amino nitrogen released during the reaction. Decreasing of amino nitrogen in the medium indicated the occurrence of a protein synthesis reaction with the formation of new covalent bonds. Were determined optimal parameters of reaction: the hydromodule, the exposure time, the concentration of EP of the preparation, were obtained mathematical models. Studies on the functional properties of composites, the physicochemical properties of the proteins that make up their composition, and structural features will make it possible to determine the uses in the manufacture of food products based on their ability to bind fat, water, form foam, gels, and etc.

scholarly journals Sublethal Effects of Crude Oil and Chemical Dispersants on Multiple Life History Stages of the Eastern Oyster, Crassostrea virginica

2020 ◽  
Vol 8 (10) ◽  
pp. 808
Author(s):  
Sara M. Garcia ◽  
Kevin T. Du Clos ◽  
Olivia H. Hawkins ◽  
Brad J. Gemmell
Keyword(s):  
Life History ◽  
Crude Oil ◽  
Crassostrea Virginica ◽  
Sublethal Effects ◽  
Oil Pollution ◽  
Eastern Oyster ◽  
Clearance Rates ◽  
Important Species ◽  
Life History Stages ◽  
Oil Exposure

The eastern oyster Crassostrea virginica is an ecologically and economically important species that is vulnerable to oil pollution. We assessed sublethal effects of soluble fractions of crude oil alone (WAF) and crude oil in combination with Corexit 9500 dispersant (CEWAF) on oysters at three life history stages. Veliger swimming, pediveliger settlement, and adult clearance rates were quantified after 24 h exposures to the contaminants. Veliger swimming speeds were not significantly impacted by 24 h exposures to WAF or CEWAF. A larger proportion of veligers were inactive following WAF and CEWAF exposure as compared to the control, but the effect was greater for pediveligers, and pediveliger settlement in the highest concentration CEWAF treatment decreased by 50% compared to controls. Thus, pediveligers may be particularly vulnerable to oil exposure. In the adults, we found significant clearance rates reductions that persisted 33 days after acute exposure to CEWAF. Knowledge of sublethal effects of oil and dispersant at multiple life history stages aids understanding of how this important species will respond to an oil spill.

Effects of acute cold exposure on muscle amino acid and protein in rats

1982 ◽  
Vol 52 (5) ◽  
pp. 1250-1256 ◽  
Author(s):  
O. L. Smith ◽  
G. Huszar ◽  
S. B. Davidson ◽  
E. Davis
Keyword(s):  
Amino Acid ◽  
Cold Exposure ◽  
Muscle Protein ◽  
Serum Insulin ◽  
Amino Nitrogen ◽  
Streptozotocin Diabetes ◽  
Total Amino Acid ◽  
Protein Breakdown ◽  
Amino Acid Release ◽  
Acid Release

To test the effects of acute cold on muscle amino acid and protein 1) rats were exposed to 4 degrees C for 24 h, functionally hepatectomized (eviscerated) and accumulation in the blood used to indicate changes in amino acid release from the tissues; 2) other rats were left intact, and urinary excretion of 3-methylhistidine (proportional to muscle protein breakdown) determined during cold exposure. In the eviscerated group, cold enhanced loss of total amino acids from the tissues (as alpha-amino nitrogen), but the loss (213 +/- 14.8% of basal in 2 h) was not due to excess alanine (180 +/- 8.5%). By comparison, in fasted rats total amino acid was 182 +/- 12.3, alanine 309 +/- 17.2%. Also, the cold-induced loss resembled the effects of streptozotocin diabetes and depended on a depression by cold of serum insulin (to 35.7 +/- 2.3 muU/ml). Therefore it was prevented when insulin was restored by infusion (40 mU . 100 g-1 . h-1) or by adrenodemedullation before cold exposure. Epinephrine (10 micrograms/100 g sc) depressed insulin in the latter and permitted amino acid release to recur. In intact rats, 3-methylhistidine excretion was unaffected by cold. The results suggest that although cold fails to stimulate alanine synthesis or protein breakdown, it inhibits insulin release sympathetically, thereby diminishing the amount of amino acid incorporated into muscle protein.

scholarly journals Ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult American lobsters Homarus americanus H. Milne Edwards, 1837 (Decapoda: Malcostraca: Nephropidae)

2019 ◽  
Vol 39 (4) ◽  
pp. 468-476 ◽  
Author(s):  
Amalia M Harrington ◽  
Heather J Hamlin
Keyword(s):  
Ocean Acidification ◽  
Carrying Capacity ◽  
Total Protein ◽  
Sublethal Effects ◽  
Homarus Americanus ◽  
Cardiac Performance ◽  
Total Protein Content ◽  
American Lobster ◽  
Control Versus ◽  
Oxygen Carrying Capacity

ABSTRACT Increased anthropogenic input of carbon dioxide into the atmosphere has caused widespread patterns of ocean acidification (OA) and increased the frequency of extreme warming events. We explored the sublethal effects of OA on the hemolymph chemistry and physiological response to acute thermal stress in the American lobster (Homarus americanus H. Milne Edwards, 1837). We exposed subadult lobsters to current or predicted end-century pH conditions (8.0 and 7.6, respectively) for 60 days. Following exposure, we assessed hemolymph L-lactate and calcium concentrations (as indicators of oxygen carrying capacity), ecdysterone concentrations, total protein content, and total hemocyte counts (THCs) as an indicator of immune response. We also assessed cardiac performance in the context of an acute warming event using impedance pneumography. Calcium, total protein, and ecdysterone concentrations were not significantly altered (P ≥ 0.10) by OA exposure. Control lobsters, however, had significantly higher levels of L-lactate concentrations compared to acidified lobsters, suggesting reduced oxygen carrying capacity under OA. THCs were also 61% higher in control versus acidified lobsters, suggesting immunosuppression under chronic OA. Lobsters exposed to acidified conditions exhibited reduced cardiac performance under acute warming as indicated by significantly lower (P = 0.040) Arrhenius Break Temperatures compared to control lobsters. These results suggest that although some physiological endpoints of American lobster are not impacted by OA, the stress of OA will likely be compounded by acute heat shock and may present additional physiological challenges for this species in the face of future change.

Renal Tubular Transport of Amino Acids

1971 ◽  
Vol 17 (4) ◽  
pp. 245-266 ◽  
Author(s):  
John Atherton Young ◽  
Benedict Sol Freedman
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Proximal Tubule ◽  
Amino Nitrogen ◽  
High Capacity ◽  
High Specificity ◽  
Brush Borders ◽  
Tubular Transport ◽  
Renal Tubular Transport ◽  
Renal Tubular

Abstract Cushny in 1917 first remarked on the extensive amino acid reabsorption which occurs in the nephron. Although many workers since then have studied the nature and localization of the reabsorptive mechanism, progress has been slow because of the technical difficulties of micropuncture work. The bulk of filtered amino nitrogen is reabsorbed in the proximal tubule although the possibility of there being more distal reabsorptive (or secretory) sites cannot be excluded. It is also uncertain whether all segments of the proximal tubule contribute equally to the reabsorptive process. Amino acid reabsorption is an active process involving numerous illdefined steps, the first of which is binding to the brush borders. Renal amino acid transport mechanisms are of two kinds: the high-capacity low-specificity systems transport whole groups of amino acids—the acidic, basic, neutral, and imino-glycine groups—while the other, the low-capacity high-specificity systems, transport single or perhaps pairs of amino acids only.

THE "BROWNING REACTION" IN DRIED MILK POWDER

1949 ◽  
Vol 27f (11) ◽  
pp. 429-434 ◽  
Author(s):  
J. B. Moster ◽  
R. A. Chapman
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Milk Powder ◽  
Amino Nitrogen ◽  
Volumetric Method ◽  
Large Excess ◽  
Browning Reaction ◽  
Titration Curves

Heated and stored dried milk powders showed a marked loss of amino nitrogen as determined by the Van Slyke volumetric method, but no loss was observed when the formol titration was used. Titration curves of the powders suggested a mechanism for the protein–sugar condensation. The heating of synthetic mixtures of amino acids and lactose resulted in intense browning, accompanied by a loss of ammo nitrogen when a large excess of lactose was employed but no loss occurred when equal parts of sugar and amino acid were present.

THE EFFECTS OF CRUDE OIL ON LARVAE OF LOBSTER HOMARUS AMERICANUS

1977 ◽  
Vol 1977 (1) ◽  
pp. 569-573
Author(s):  
Joseph M. Forns
Keyword(s):  
Crude Oil ◽  
Mass Culture ◽  
Homarus Americanus ◽  
Behavioral Characteristics ◽  
American Lobster ◽  
Larval Stages ◽  
Fourth Larval Stage ◽  
Flow Through ◽  
Oil Exposure ◽  
South Louisiana

ABSTRACT The effects of API reference South Louisiana crude oil upon four larval stages of American lobster (Homarus americanus) were determined in a flow-through system. Tests were conducted with naturally-hatched animals in individual test chambers as well as in mass culture systems in an operating state lobster hatchery. Experimental flow-through crude oil exposure concentrations were 0.1, and 1.0 ppm, administered as a strongly-agitated emulsion-like mix to ambient temperature seawater ranging from 15°-20°C. Oil exposure residence times ranged from 0.8-5.6 minutes depending on the test. Exposed animals were monitored six times daily for feeding behavioral characteristics, mobility, molting success, growth and development times to reach the fourth larval stage. Pigmentation analysis was performed on individual larvae by photomicroscopy, and hydrocarbon analyses were also conducted thereon. Post-larval development through the eighth stage was investigated. Statistical comparisons were made among different control animals and between control and oil-exposed larvae.

Dietary Intake and Nutritional Characteristics in Wild American Lobsters (Homarus americanus)

1979 ◽  
Vol 36 (8) ◽  
pp. 965-969 ◽  
Author(s):  
D. F. Leavitt ◽  
R. C. Bayer ◽  
M. L. Gallagher ◽  
J. H. Rittenburg
Keyword(s):  
Amino Acid ◽  
Essential Amino Acid ◽  
Crude Protein ◽  
Stomach Contents ◽  
Homarus Americanus ◽  
Molt Cycle ◽  
Nutritional Characteristics ◽  
Gross Energy ◽  
Mineral Constituent ◽  
The Relationship

Nutrient and essential amino acid analyses of the stomach contents of wild adult American lobsters (Homarus americanus) collected from offshore and inshore populations indicated lobsters consumed varying nutrient levels according to their stage of molt. For example, soft-shell lobsters ate a higher level of mineral constituent. The location of the animal had no effect on the level of nutrient intake. The essential amino acid composition of the protein in the stomach remained the same throughout the molt cycle, but indicated a change in protein source with the location of the animal. The relationship between crude protein and gross energy was constant suggesting its importance in the nutrition of the lobster. Based on this study, we recommend a basal level of nutrient constituents for ration formulation. Key words: American lobster, natural intake, dietary formulation

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