Uptake of Cadmium in Perfused Rainbow Trout (Salmo gairdneri) Gills

1981 ◽  
Vol 38 (8) ◽  
pp. 917-924 ◽  
Author(s):  
P. Pärt ◽  
O. Svanberg
Keyword(s):  
Rainbow Trout ◽  
Metal Uptake ◽  
Fold Increase ◽  
Salmo Gairdneri ◽  
Complexing Agent ◽  
Heavy Metal Uptake ◽  
Perfusion Technique ◽  
Factors Affecting ◽  
In Vitro Techniques

Uptake of cadmium in gills from rainbow trout (Salmo gairdneri) has been studied by the head perfusion technique. The importance of checking the viability of the perfused gills is considered and some viability criteria are discussed. The results show that cadmium is transferred through perfused gills. A 10-fold increase in the external Cd concentration from 5 × 10−8 to 5 × 10−7 mol∙L−1 resulted in a nearly 100-fold increase in cadmium transfer. At higher cadmium concentrations the uptake rate of the element diminished after having reached a maximum within 30 min. A complexing agent, EDNTA, which strongly chelates free cadmium ions decreases the transfer of cadmium through the gills. The advantage of using in vitro techniques instead of conventional uptake experiments is discussed and it is concluded that the gill perfusion technique is a useful tool in studies of factors affecting heavy metal uptake in fish.Key words: perfusion technique, fish gills, Salmo gairdneri, cadmium uptake, viability, EDNTA

scholarly journals Some effects of vitamin E and selenium deprivation on tissue enzyme levels and indices of tissue peroxidation in rainbow trout (Salmo gairdneri)

1985 ◽  
Vol 53 (1) ◽  
pp. 149-157 ◽  
Author(s):  
J. G. Bell ◽  
C. B. Cowey ◽  
J. W. Adron ◽  
Aileen M. Shanks
Keyword(s):  
Rainbow Trout ◽  
Vitamin E ◽  
Peroxidase Activity ◽  
Cumene Hydroperoxide ◽  
Dietary Vitamin ◽  
Salmo Gairdneri ◽  
Deficient Diet ◽  
Exudative Diathesis ◽  
Malondialdehyde Formation

1. Duplicate groups of rainbow trout (Salrno gairdnert) (mean weight 11 g) were given for 40 weeks one of four partially purified diets that were either adequate or low in selenium or vitamin E or both.2. Weight gains of trout given the dually deficient diet were significantly lower than those of trout given a complete diet or a diet deficient in Se. No mortalities occurred and the only pathology seen was exudative diathesis in the dually deficient trout.3. There was significant interaction between the two nutrients both with respect to packed cell volume and to malondialdehyde formation in the in vitro NADPH-dependent microsomal lipid peroxidation system.4. Tissue levels of vitamin E and Se decreased to very low levels in trout given diets lacking these nutrients. For plasma there was a significant effect of dietary vitamin E on Se concentration.5. Glutathione (GSH) peroxidase (EC 1. 1 1. 1.9) activity in liver and plasma was significantly lower in trout receiving low dietary Se but was independent of vitamin E intake. The ratios of hepatic GSH peroxidase activity measured with cumene hydroperoxide and hydrogen peroxide were the same for all treatments. This confirms the absence of a Se-independent GSH peroxidase activity in trout liver.6. Se deficiency did not lead to any compensatory increase in hepatic GSH transferase (EC 2. 5. 1. 18) activity; values were essentially the same in all treatments.7. Plasma pyruvate kinase (EC 2. 7. 1.40) activity increased significantly in the trout deficient in both nutrients. This was thought to be due to leakage of the enzyme from the muscle and may be indicative of incipient (subclinical) muscle damage.

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