Viruses Associated with Lymphocystis Disease and Dermal Sarcoma of Walleye (Stizostedion vitreum vitreum)

1976 ◽  
Vol 33 (11) ◽  
pp. 2408-2419 ◽  
Author(s):  
Tats Yamamoto ◽  
Richard D. Macdonald ◽  
Douglas C. Gillespie ◽  
Russell K. Kelly
Keyword(s):  
Electron Microscopic Examination ◽  
The Body ◽  
Stizostedion Vitreum ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Large Numbers ◽  
Lymphocystis Disease ◽  
Spawning Run ◽  
Tumor Types ◽  
Dermal Sarcoma

Gross examination of a spawning run of walleye (Stizostedion vitreum vitreum) showed a large proportion of fish to have tumors on the body and fins that appeared to be characteristic of lymphocystis disease. Light and electron microscopic examination revealed the presence of two distinct tumor types. One was characteristic of lymphocystis, consisting of typical enlarged nonneoplastic cells surrounded by hyaline layers and containing many 260 nm diameter lymphocystis virus particles in the cytoplasm. The other tumor, referred to as a dermal sarcoma, consisted of a solid mass of normal-sized cells and contained in the cytoplasm large numbers of 135 nm diameter virus particles referred to as walleye dermal sarcoma (WDS) virus. The WDS virus was similar in appearance to the leukoviruses and, with its outer layer sectioned tangentially, exhibited symmetry like a member of a leukovirus group designated by Fenner as subgenus C.

An Ultrastructural Study of Interspecific Cell Fusion Induced by Inactivated Sendai Virus

1966 ◽  
Vol 1 (4) ◽  
pp. 401-406
Author(s):  
E. E. SCHNEEBERGER ◽  
H. HARRIS
Keyword(s):  
Cell Fusion ◽  
Hela Cells ◽  
Sendai Virus ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Large Numbers ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells ◽  
Cytoplasmic Bridges

An electron-microscopic examination was made of the process of cell fusion induced by Sendai virus inactivated by ultraviolet light. Ehrlich ascites cells, HeLa cells, rabbit macrophages, rat lymphocytes and nucleated hen erythrocytes were chosen for study because it had previously been shown that these cells could be fused together, with varying degrees of facility, to form artificial heterokaryons. Cells which had large numbers of microvilli on their surfaces fused together more readily than those which had not, but the presence of microvilli was not essential for fusion to occur. Fusion appeared in all cases to be initiated by the formation of small cytoplasmic bridges between the cells; but virus particles, although present elsewhere on the surface of the cells, were not detected at or near the cytoplasmic bridges. HeLa-hen erythrocyte heterokaryons were formed by the fusion of HeLa cells with red cell ghosts.

scholarly journals Role of Helicobacter felis in Chronic Canine Gastritis

1992 ◽  
Vol 29 (6) ◽  
pp. 487-494 ◽  
Author(s):  
A. Lee ◽  
S. Krakowka ◽  
J. G. Fox ◽  
G. Otto ◽  
K. A. Eaton ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Plasma Cells ◽  
Serum Antibody ◽  
Electron Microscopic Examination ◽  
Gastric Mucus ◽  
Electron Microscopic ◽  
Helicobacter Felis ◽  
Large Numbers ◽  
Histopathologic Changes ◽  
Gastric Parietal Cells

Five gnotobiotic Beagle dogs were orally inoculated with a pure culture of Helicobacter felis. The remaining two littermates served as contact controls. Thirty days after infection, all animals were euthanatized and specimens were collected for evaluation. In infected dogs, H. felis was recovered from all areas of the stomach. Colonization was heaviest in the fundus and antrum. H. felis was not cultured from any segment of the gastrointestinal tract distal to the duodenum. Two weeks after infection, all five infected dogs had detectable IgM and IgG serum antibody to H. felis, whereas control dogs had no measurable H. felis serum antibody throughout the study. Histopathologic changes in the stomachs of infected dogs included large numbers of lymphoid nodules throughout all regions of the gastric mucosa and were most numerous in the fundus and body. A mild, diffuse lymphocytic infiltrate with small numbers of plasma cells and eosinophils was also present in the subglandular region of all portions of the gastric mucosa. Electron microscopic examination revealed large numbers of spiral-shaped H. felis in gastric mucus adjacent to or superimposed over the areas of inflammation. Occasionally, however, H. felis was observed within the canaliculi of gastric parietal cells. Histopathologic changes in the stomachs of the contact control dogs were limited to focal infiltrates of eosinophils and small aggregates of lymphocytes in the subglandular portions of the gastric mucosa in one animal. Infection with H. felis is a likely cause of naturally occurring lymphofollicular gastritis.

Watermelon mosaic virus 1 and 2 in Queensland cucurbit crops

1978 ◽  
Vol 29 (6) ◽  
pp. 1235 ◽  
Author(s):  
RS Greber
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Electron Microscopic Examination ◽  
Watermelon Mosaic Virus ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Cellular Inclusions ◽  
Differential Hosts

Watermelon mosaic virus type 1 (WMV-1) has not previously been reported from Australia and has become prevalent in Queensland only since 1970. Watermelon mosaic virus type 2 (WMV-2) continues to reach a high incidence in marrow (Cucurbita pepo) and pumpkin (Cucurbita maxima and Cucurbita moschata) crops, WMV-2 is rarely isolated from watermelons (Citrullus vulgaris) in which epidemics of WMV-1 now cause serious reductions in yield. The Queensland Blue cultivar of C. maxima, the most important cucurbit crop, produces severely distorted fruit following infection by WMV-1, although it is little affected by WMV-2. Physical properties of these WMV isolates and electron microscopic examination of the virus particles and associated cellular inclusions showed them to be similar to those reported elsewhere, but there were some distinctive host reactions for the WMV-2 isolates. No resistance to either WMV-1 or WMV-2 was found in commercially available C. pepo, C. maxima or C. vulgaris. The resistance of Cucumis metuliferus to Australian WMV-1 isolates was confirmed, and a source of resistance to both WMV-1 and WMV-2 was found in Lagenaria siceravia. Methods of separation of WMV-1 and WMV-2 from mixed isolates and methods for the identification of each on differential hosts and by serology were shown to be effective.

scholarly journals Using of the method of electron microscopy for the identification and analysis of clinically implicit pathogenetic manifestations

2018 ◽  
Vol 7 (3) ◽  
pp. 113-116
Author(s):  
L. G. Nikonova ◽  
V. V. Banin ◽  
I. G. Stel'nikova
Keyword(s):  
Diabetes Mellitus ◽  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
B Cells ◽  
Secretory Granules ◽  
Electron Microscopic Examination ◽  
The Body ◽  
Electron Microscopic ◽  
Latent Form ◽  
Insulin Cells

Electron microscopic examination of B cells of pancreatic islets of the pancreas in dogs with normal (n=10) and impaired glucose tolerance (n=10) was performed. Ultrastructural features of the organization of insulin cells associated with an increased requirement of the hormone in the body with the latent form of diabetes mellitus are established. In B cells, signs of functional tension due to unregulated secretion, manifested by the expansion of endoplasmic reticulum cisterns, Golgi complex hypertrophy, an increase in the number of immature secretory granules and vacuoles in the cytoplasm are revealed in B cells.

Peculiarities of the ultrastructure of mixed biofilms of the causing agent of diphtheria and conditionally pathogenic microorganisms isolated from the human respiratory tract

2021 ◽  
Vol 66 (10) ◽  
pp. 623-628
Author(s):  
V. N. Gerasimov ◽  
Galina Georgievna Kharseeva ◽  
O. S. Sherbataya ◽  
S. A. Kotov ◽  
A. V. Chepusova
Keyword(s):  
Respiratory Tract ◽  
Electron Microscopic Examination ◽  
The Body ◽  
Resistant Bacteria ◽  
Bacterial Cells ◽  
Human Respiratory Tract ◽  
Electron Microscopic ◽  
Opportunistic Bacteria ◽  
Epidemic Period ◽  
High Level

In the post-epidemic period, the circulation of the causative agent of diphtheria in the population is maintained due to the carrier of bacteria. Entering an organism with a high level of antitoxic immunity, the pathogen enters into intermicrobial interactions with representatives of the opportunistic microflora inhabiting the respiratory tract and forms a biofilm. Materials and methods. Modeling of the biofilm formation process was carried out using the strains C.diphtheriae gravis tox+№. 665, C.pseudodiphtheriticum, S.aureus. Biofilm samples were placed on the stage of a scanning electron microscope and gold-sputtered in an EicoIB-3 ioncoater vacuum deposition unit (Eico, Japan) at an ion current of 6-8 mA. The samples obtained were examined in a JEOL 6510LB scanning electronmicroscope. («JEOL» company, Japan) at an accelerating voltage of 30 kV. Results. Electron microscopic examination of samples of biofilms C. diphtheriae gravis tox+ № 665 and opportunistic microorganisms shows groups of 2-7 young bacterial cells packed into a single microcapsule. Much more voluminous accumulations of bacterial cells (more than 10-12) are typical for biofilm samples represented by C. diphtheriae gravis tox+№ 665 and S. aureus cells. On the surface of the biofilm, young bacterial cells with an intact structure are located at various stages of active division. The conglomerates of bacterial cells, covered with a common intermicrobial matrix, adhere tightly to each other and form a multilayer biofilm. Conclusion. Features of the ultrastructure of biofilms containing strains of C. diphtheriae and opportunistic bacteria, especially antibiotic-resistant bacteria inhabiting the respiratory tract, can contribute to long-term persistence of the pathogen of diphtheria in the body. They not only significantly complicate the access of antibacterial drugs, but also interfere with the isolation of C.diphtheriae during bacteriological research.

scholarly journals First Report of Occurrence of Papaya ring spot virus Infecting Papaya in Bangladesh

Plant Disease ◽  
2004 ◽  
Vol 88 (2) ◽  
pp. 221-221 ◽  
Author(s):  
R. K. Jain ◽  
K. M. Nasiruddin ◽  
Jyoti Sharma ◽  
R. P. Pant ◽  
A. Varma
Keyword(s):  
Polyclonal Antibodies ◽  
Electron Microscopic Examination ◽  
Aphid Transmission ◽  
Electron Microscopic ◽  
Spot Virus ◽  
First Report ◽  
Virus Particles ◽  
Cp Gene ◽  
Papaya Ring Spot Virus ◽  
Ring Spot

Papaya (Carica papaya L.) is an important fruit crop in Bangladesh. During surveys conducted in Dhaka and Mymensingh regions from April to June 2003, >50% of papaya plants were observed to have leaf mottling, mosaic and mild distortion, and water-soaked streaks on petioles and stem, which are typical symptoms of Papaya ring spot virus (PRSV) infection. Electron-microscopic examination of negatively stained leaf-dip preparations from 10 symptomatic samples revealed the association of flexuous virus particles that were decorated with polyclonal antibodies raised to an isolate from India (PRSV-D). The identity of PRSV associated with the papaya disease in Bangladesh was further confirmed by reverse transcription polymerase chain reaction and sequence analysis (2). By using PRSV specific primers (2), the 3′-terminal region comprising a part of the nuclear inclusion b gene, the coat protein (CP) gene, and the untranslated region were amplified and sequenced (GenBank Accession No. AY423557). The CP gene consisted of 286 amino acids and the conserved regions common to the genus Potyvirus, such as WCIEN and QMKAA, were present. Like all known PRSV sequences (1), a stretch of glutamic acid and lysine repeats (EK region) after the aphid transmission motif (DAG) also was present. Comparative CP amino acid sequence analyses revealed that the virus infecting papaya in Bangladesh, designated as PRSV-Bd, shared 89 to 92% identity with PRSV isolates from India and 88 to 93% identity with isolates from other parts of the world. To our knowledge, this is the first report of occurrence of PRSV infecting papaya in Bangladesh. References: (1) M. F. Bateson et al. J. Gen. Virol. 83:2575, 2002. (2) R. K. Jain et al. Ann Appl. Biol. 132:413, 1998.

Dentirumai philippinensis n. gen., n. sp. (Nematoda: Philometridae), a new tissue-infecting philometrid nematode from the loach goby Rhyacichthys aspro (Valenciennes) (Rhyacichthyidae) in the Philippines

2012 ◽  
Vol 87 (1) ◽  
pp. 59-65 ◽  
Author(s):  
K.M.A. Quiazon ◽  
F. Moravec
Keyword(s):  
New Genus ◽  
Electron Microscopic Examination ◽  
Nematode Species ◽  
Body Cavity ◽  
The Philippines ◽  
The Body ◽  
Electron Microscopic ◽  
Oral Aperture ◽  
Body Tissues ◽  
Subcutaneous Tissues

AbstractA new genus and species of the philometrid nematode Dentirumaiphilippinensis n. gen., n. sp. Philometrinae, Philometridae, are described on the basis of the female specimens found in the loach goby, Rhyacichthys aspro (Valenciennes), from the upper Bianuan River, Municipality of Casiguran, Aurora Province, in the Philippine Archipelago. Dentirumai philippinensis was collected from the body cavity, subcutaneous tissues near the bases of the pectoral and pelvic fins, and surrounding body tissues in the hypaxial musculature of R. aspro. Based on light microscopic and scanning electron microscopic examination, the new nematode species is morphologically close to the genus Rumai Travassos, 1960 except for one major difference, i.e. the presence of a sclerotized ring in the oral aperture armed with distinct numerous minute teeth on its inner surface. This important taxonomic feature makes it possible to erect a new genus, Dentirumai n. gen. to accommodate the newly discovered philometrid species. A key to genera of the Philometrinae is provided.

An Electron Microscopic Study of Mast Cells in Human Kidneys

1973 ◽  
Vol 31 ◽  
pp. 416-417
Author(s):  
A. K. Mandal ◽  
J. A. Nordquist ◽  
R. C. Muehrcke ◽  
F. I. VoIini
Keyword(s):  
Electron Microscopy ◽  
Urinary Tract ◽  
Mast Cells ◽  
Inflammatory Diseases ◽  
Percutaneous Biopsy ◽  
The Body ◽  
Electron Microscopic ◽  
Chronic Inflammatory Diseases ◽  
Urinary Tract Disorders ◽  
Large Numbers

Mast cells are widely distributed throughout the body and normally are present in almost every organ. Although mast cells are absent in normal kidneys, large numbers have been reported in kidneys with chronic inflammatory diseases. The granules in cutaneous mast cells show a positive flourescence for dopamine and norepinephrine suggesting that these cells may contain vasopressor substances. The purpose of these studies was to determine the characteristics and frequency of mast cells in the kidneys of patients with renal disease and hypertension.Renal tissues from 25 patients with a variety of renal and urinary tract disorders were studied using electron microscopy (RCA, EMU3H). Tissue was obtained by percutaneous biopsy in 17 cases, by nephrectomy in 6 and by autopsy in 2.

scholarly journals Rhabdias bufonis (Rhabdiasidae) from the lung of the African common toad, Amietophrynus regularis (Bufonidae) in Egypt. New data on the basis of light and scanning electron microscopic study

2018 ◽  
Author(s):  
Kareem Morsy ◽  
Sara Ali ◽  
Fathy Abdel Ghaffar ◽  
Hoda El Fayoumi ◽  
Heba Abdel Haleem
Keyword(s):  
Natural Habitat ◽  
Mouth Opening ◽  
Electron Microscopic Examination ◽  
The Body ◽  
Morphological Description ◽  
Common Toad ◽  
Electron Microscopic ◽  
Nematode Parasites ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Background and Aims. Rhabdias bufonis (Rhabdiasidae) is one of the highly pathogenic nematode parasites infecting the lung of amphibians. The present study provides the morphological description of this nematode isolated from the lung of the African common toad, Amietophrynus regularis collected from its natural habitat; the damp, moist fields and gardens at Giza governorate, Egypt. The description was based on the data obtained from light and scanning electron microscopic examination. Methods. Forty specimens of the examined animals were collected during the period from March to August 2017. At necropsy, the toads were dissected ventrally from the cloacae region to the anterior end and all organs were examined searching for helminthes using a stereomicroscope. Results. Of 40 examined specimens 14 (35%) were found to harbor a large number of this parasite. All the recovered worms were females and measuring 3.22-9.86 mm long and 0.09-0.048 mm wide at mid body. The anterior end was blunted while the posterior one was tapered. The body was covered by a delicate inflation of the cuticle strongly folded on its surface. Conclusions. The SEM study presented new details regarding the cephalic end of this nematode which was not identified in the previous studies, of them, a slit-like mouth surrounded by two pairs of lateral papillae and two amphids. Also, three pairs of cuticular inflation supporting the area around mouth opening were observed

scholarly journals ULTRASTRUCTURE AND HEPATOCYTE KARYOCYTOMETRY OF QUAILS

2019 ◽  
Vol 51 (3) ◽  
pp. 36-41
Author(s):  
G.Z. Bronnikova ◽  
◽  
E.N. Skovorodin ◽  
Keyword(s):  
Microscopic Examination ◽  
Morphological Characteristics ◽  
Electron Microscopic Examination ◽  
Cell Ultrastructure ◽  
The Body ◽  
Qualitative Description ◽  
Synthetic Activity ◽  
Electron Microscopic ◽  
Pharmacological Agents ◽  
The Impact

The paper studies the functional morphology of quail hepatocytes. To conduct the research there was an electron-microscopic examination of quail liver. The structure of the nucleus and nucleolus were quantitatively assessed using objective methods of karyocytometry. Quail hepatocytes are found to have high synthetic activity. Hepatocyte karyocytometry enables to reveal hidden morphological characteristics of the cell ultrastructure being hardly detected under normal qualitative description of electron-diffraction photos. Quail hepatocyte karyocytometry indicates a pronounced structural and functional heterogeneity of hepatocytes. Therefore, electron-microscopic examination using karyocytometric methods is a highly informative method for assessing the morphological and functional state of the body and it should be used in assessing the impact of pharmacological agents on the body.

Sign in / Sign up

Export Citation Format

Share Document