Short-Term Serum Prolactin Concentrations in Goldfish (Carassius auratus) Subjected to Serial Sampling and Restraint

1976 ◽  
Vol 33 (1) ◽  
pp. 183-186 ◽  
Author(s):  
Richard E. Spieler ◽  
Albert H. Meier

Serum prolactin concentrations in serially sampled goldfish (Carassius auratus) did not vary significantly between 30 s and 3 min after initial capture. A marked decrease in prolactin concentration was noted 9–17 min after initial capture followed by a recovery at 30–48 min. Although there was a circadian variation in prolactin concentration, the pattern of response to capture and sampling did not vary significantly among the times tested.

1974 ◽  
Vol 31 (7) ◽  
pp. 1240-1242 ◽  
Author(s):  
Richard E. Spieler

Serum cortisol from serially sampled goldfish (Carassius auratus) did not demonstrate a stress-evoked increase (P >.05) until 10–22 min after initial capture. This response did not differ (P > 0.5) among fish sampled at the beginning, middle, and end of a 12-h light period.


2017 ◽  
Vol 49 (2) ◽  
pp. 726-737 ◽  
Author(s):  
Ping Wu ◽  
Jun Shi ◽  
Chengyong Yang ◽  
Fangliang Zhang ◽  
Yulong Li ◽  
...  

1980 ◽  
Vol 212 (3) ◽  
Author(s):  
RichardE. Peter ◽  
Olivier Kah ◽  
ChristineR. Paulencu ◽  
Harry Cook ◽  
AnnL. Kyle

1977 ◽  
Vol 55 (8) ◽  
pp. 1304-1311 ◽  
Author(s):  
R. M. Walker ◽  
P. H. Johansen

At 20 °C goldfish survive anaerobic conditions for only a few hours while at 4 °C survival is extended to several days. During the course of low-temperature anaerobiosis there was a rise in blood glucose and lactate, a decline in liver glycogen concentration, and an increase in liver water content, while liver size remained constant.The better cold anaerobic survival of winter and hypophysectomized goldfish compared with spring and sham-operated animals was correlated with greater glycogen stores in the livers of the former. It is concluded that liver glycogen is a necessary energy source during cold anaerobiosis, and it is suggested that the resulting hyperglycemia may represent a mechanism to increase glycolytic energy yield. Cold anaerobiosis also resulted in elevated liver glucose-6-phosphatase (EC 3.1.3.9) activity, suggesting an increase in glycogenolysis, but no change in glycogen phosphorylase (EC 2.4.1.1) activity. While cold anaerobic survival is short term it is possible that liver glycogen may sustain goldfish for longer periods at low oxygen levels through a mixed aerobic–anaerobic metabolism.


1975 ◽  
Vol 32 (9) ◽  
pp. 1589-1594 ◽  
Author(s):  
Chris P. Archibald

The short-term effects of various intensities offish predation on zooplankton communities were examined. Field studies indicated that fish predation was, as expected, size-selective: in the presence of fish, size distributions of the principal prey, Daphnia pulex, were skewed toward the smaller size-classes and the minimum size of egg-bearing D. pulex was also smaller. Fecundity of Diaptomus ashlandi, and probably also of Daphnia pulex, was greater with fish present. This caused the abundance of these two dominant species to increase initially with increasing predation. Subsequently, the species declined at higher predation levels in order of their interest to fish, with large D. pulex[Formula: see text] declining before small D. pulex (< 1.35 mm) and both groups doing so at lower predation levels than Diaptomus ashlandi.


1987 ◽  
Vol 72 (6) ◽  
pp. 737-741 ◽  
Author(s):  
D. J. Kerr ◽  
V. K. Singh ◽  
M. G. McConway ◽  
G. H. Beastall ◽  
J. M. C. Connell ◽  
...  

1. The effect of low dose dopamine infusion on the circadian rhythm of thyrotrophin (TSH), prolactin and Cortisol in a group of six healthy male volunteers is reported. Subjects were infused in random order with either saline (154 mmol/l NaCl solution; control) or dopamine (0.1 and 1 μg min−1 kg−1) between 21.00 and 01.00 hours, in random order. 2. The serum TSH profile was characterized by a maximal peak occurring at 23.00 hours and higher nocturnal than diurnal values. Superimposed on this are short term oscillations in serum TSH levels, typical of an ultradian rhythm. 3. The maximal peak in TSH, occurring at 23.00 hours, was abolished by dopamine infused at a rate of 1 μg min−1 kg−1, and was unaffected by the lower rate of dopamine infusion (0.1 μg min−1 kg−1). 4. The serum prolactin profile was characterized by a peak occurring soon after the onset of sleep (23.30–00.30 hours), which fell during the morning, and began to rise in late evening. 5. Low dose dopamine (0.1 μg min−1 kg−1) had a slight but insignificant effect with decreased prolactin levels at the end of the infusion whereas the higher dopamine dose was associated with significantly lower prolactin levels during and throughout the infusion. There was a rebound to levels significantly higher than control on cessation of the infusion. Cortisol levels were unaffected by dopamine.


1976 ◽  
Vol 54 (11) ◽  
pp. 1960-1968 ◽  
Author(s):  
B. A. McKeown ◽  
R. E. Peter

A number of experiments were conducted to investigate the effects of photoperiod and temperature on prolactin release from the goldfish pituitary gland. Fish were acclimated to different photoperiods and temperatures, and also were subjected to a change in either of these two parameters after different acclimation conditions. Serum and pituitary samples were collected and analyzed by radioimmunoassay for prolactin levels. In other experiments samples for prolactin analysis were taken every 3 h intermittently over a period of 3 days from fish that were acclimated to different photoperiod and temperature conditions. Longer photoperiods and higher temperatures caused pituitary prolactin release. Serum prolactin changed on a circadian rhythm and the rhythm was modified depending on the length of the photoperiod.


Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.


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