Fluorescent Antibody Identification and Detection of the Corynebacterium Causing Kidney Disease of Salmonids

1975 ◽  
Vol 32 (11) ◽  
pp. 2224-2227 ◽  
Author(s):  
G. L. Bullock ◽  
H. M. Stuckey
Keyword(s):  
Kidney Disease ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serological Identification

An indirect fluorescent antibody technique (FAT) has been developed for serological identification, diagnosis of kidney disease (KD), and detection of subclinical infections of KD. Rabbit or goat antikidney disease serum and appropriately labeled immunoglobulin was found to be specific for the KD bacterium. The technique was superior to examination of gram-stained smears for detection of small numbers of KD bacteria. The test requires approximately 2 h.

Detection of Bacterial Kidney Disease in Wild Salmonids in the Margaree River System and Adjacent Waters Using an Indirect Fluorescent Antibody Technique

1979 ◽  
Vol 36 (12) ◽  
pp. 1464-1468 ◽  
Author(s):  
W. D. Paterson ◽  
C. Gallant ◽  
D. Desautels ◽  
L. Marshall
Keyword(s):  
Kidney Disease ◽  
Atlantic Salmon ◽  
Fluorescent Antibody ◽  
River System ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Bacterial Kidney Disease ◽  
Key Words Kidney ◽  
Asymptomatic Infections

The indirect fluorescent antibody technique demonstrated the enzootic nature of bacterial kidney disease in 224 of the 605 wild salmonids sampled from the Margaree River system and some adjacent waters. Asymptomatic infections were observed in 33.4% of 456 Atlantic salmon (Salmo salar) parr and 35.1% of 37 returning adults in samples taken from the source of the Margaree River to and including saltwater areas off the coast. Key words: kidney disease, Atlantic salmon, wild, distribution, fluorescent antibody

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

scholarly journals Toxoplasma antibodies in immigrants from Hong Kong

1974 ◽  
Vol 72 (1) ◽  
pp. 19-22 ◽  
Author(s):  
S. K. K. Seah
Keyword(s):  
Hong Kong ◽  
Toxoplasma Gondii ◽  
Indigenous Population ◽  
Fluorescent Antibody ◽  
Age Groups ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Chinese Adults ◽  
Antibody Technique ◽  
Total Incidence

SUMMARYThe sera of 200 Chinese adults recently immigrated to Canada from Hong Kong were examined for antibodies to Toxoplasma gondii by the indirect fluorescent antibody technique. It was found that the total incidence was 18·0%. This is significantly lower than that of the indigenous population of the same age groups in this area.

scholarly journals Diagnosis of Avian Infectious Bronchitis by Indirect Fluorescent Antibody Technique Using Monoclonal Antibody

1993 ◽  
Vol 46 (2) ◽  
pp. 117-119 ◽  
Author(s):  
Kazuki ISHIBASHI ◽  
Hitomi SHIRAKAWA ◽  
Yoshifumi TOMISHITA ◽  
Hiroyuki MATUO ◽  
Akira WATANABE ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Infectious Bronchitis

scholarly journals Fluorescent antibody test for the serological diagnosis of gonorrhea

1976 ◽  
Vol 3 (4) ◽  
pp. 438-442
Author(s):  
H A Gaafar ◽  
D C D'Arcangelis
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Immunoglobulin G ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Serological Diagnosis ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Normal Individuals ◽  
Male Patients

An indirect fluorescent antibody technique has been developed for the serological diagnosis of gonorrhea. The selected strain(s) of Neisseria gonorrhoeae possesses a heat-labile surface antigen (L-antigen). Sera are diluted 1:10, and an aliquot is hear inactivated at 59 C for 30 min. The treated and untreated aliquots are then examined for human immunoglobulin G anti-L-antigen. In a prelimiary study of 495 sera, 95% of those from women with a bacteriologically confirmed diagnosis of gorrhea and 87% of those from male patients were reactive in this test, whereas only 1.4% fo the sera from presumably normal individuals were reactive.

Sign in / Sign up

Export Citation Format

Share Document