Thiobarbituric Acid Value, Total Long-Chain Free Fatty Acids, and Flavor of Pacific Halibut (Hippoglossus stenolepis) and Chinook Salmon (Oncorhynchus tshawytscha) Frozen at Sea

1973 ◽  
Vol 30 (1) ◽  
pp. 63-69 ◽  
Author(s):  
J. R. Botta ◽  
J. F. Richards ◽  
N. Tomlinson

The effects of brine-freezing and plate-freezing at sea upon 2-thiobarbituric acid (TBA) values, concentration of long-chain free fatty acids (FFA), and flavor of Pacific halibut and chinook salmon were determined at intervals during subsequent storage of the glazed, polyethylene covered fish at − 30 C. Evaluations were conducted on both outside and inside muscle.Brine-frozen samples of outside muscle generally had higher TBA values than plate-frozen samples and the differences were most pronounced at intermediate storage times (26–45 weeks).Method of freezing significantly (P ≤ 0.05) affected the concentration of total FFA in Pacific halibut, and the total FFA concentration of both Pacific halibut and chinook salmon significantly increased with length of frozen storage. For both species the concentration of total FFA was significantly greater in outside than in inside muscle.Significant flavor differences between brine- and plate-frozen samples of outside muscle were evident at intermediate storage times for both species. For inside muscle of halibut, the significance of flavor differences generally increased with storage time whereas flavor differences for inside muscle of salmon were consistently nonsignificant.Taste panel results and TBA values indicated that, in comparison to plate-freezing, brine-freezing impaired the quality of outside muscle of Pacific halibut and chinook salmon during the early stages of frozen storage.

1973 ◽  
Vol 30 (1) ◽  
pp. 79-82
Author(s):  
J. R. Botta ◽  
J. F. Richards ◽  
N. Tomlinson

Changes in the concentration of long-chain free fatty acids were determined gas chromatographically from inside and outside muscle samples of brine- or plate-frozen Pacific halibut and chinook salmon stored for 9–81 weeks.With both species the concentration of individual free fatty acids was greater in outside muscle than in inside muscle. Few differences in concentration of free fatty acids were noted between plate- and brine-frozen samples. Numerous free fatty acids increased significantly in concentration during frozen storage. For both species the increase was most rapid during the first 26–45 weeks of storage. The detection of flavor differences was unrelated to the concentration of individual free fatty acids.


2019 ◽  
Vol 32 (2) ◽  
pp. 16-22
Author(s):  
Alaa M.S. Al-Baidhani ◽  
Aum El-Bashar H.J. Al-Mossawi

This study included preparation of ostrich meat burger with different levels of ostrich fat. The first treatment was free-fat and the second treatment 5% fat, the third treatment was 10% fat, the fourth and the fifth was 15% and 20% respectively and stored in 18 ± 2 co for 120 days. Changes in chemical indicators were studied including peroxide value (PV), thiobarbituric acid (TBA), free fatty acids (FFA) and total volatile nitrogen (TVN) during storage periods 1, 30, 60, 90 and 120 days. The results showed that there is significantly increased (P <0.05) in PV, TBA and FFA by increasing the fat levels and the storage periods while TVN decreased by increasing fat levels and increased storage periods.


2020 ◽  
Vol 202 (15) ◽  
Author(s):  
Patrícia T. dos Santos ◽  
Rikke S. S. Thomasen ◽  
Mathias S. Green ◽  
Nils J. Færgeman ◽  
Birgitte H. Kallipolitis

ABSTRACT Naturally occurring free fatty acids (FFAs) are recognized as potent antimicrobial agents that also affect the production of virulence factors in bacterial pathogens. In the foodborne pathogen Listeria monocytogenes, some medium- and long-chain FFAs act as antimicrobial agents as well as signaling compounds, causing a repression of transcription of virulence genes. We previously observed that the master virulence regulator PrfA is involved in both the antimicrobial and virulence-inhibitory response of L. monocytogenes to selected FFAs, but the underlying mechanisms are presently unknown. Here, we present a systematic analysis of the antimicrobial and PrfA-inhibitory activities of medium- and long-chain FFAs of various carbon chain lengths and degrees of saturation. We observed that exposure to specific antimicrobial and nonantimicrobial FFAs prevented PrfA-dependent activation of virulence gene transcription and reduced the levels of PrfA-regulated virulence factors. Thus, an antimicrobial activity was not compulsory for the PrfA-inhibitory ability of an FFA. In vitro binding experiments revealed that PrfA-inhibitory FFAs were also able to prevent the constitutively active variant PrfA* from binding to the PrfA box in the promoter region of the virulence gene hly, whereas noninhibitory FFAs did not affect its ability to bind DNA. Notably, the unsaturated FFAs inhibited the DNA binding activity of PrfA* most efficiently. Altogether, our findings support a model in which specific FFAs orchestrate a generalized reduction of the virulence potential of L. monocytogenes by directly targeting the key virulence regulator PrfA. IMPORTANCE Listeria monocytogenes is a Gram-positive pathogen able to cause foodborne infections in humans and animals. Key virulence genes in L. monocytogenes are activated by the transcription regulator PrfA, a DNA binding protein belonging to the CRP/FNR family. Various signals from the environment are known to affect the activity of PrfA, either positively or negatively. Recently, we found that specific medium- and long-chain free fatty acids act as antimicrobial agents as well as signaling compounds in L. monocytogenes. Here, we show that both antimicrobial and nonantimicrobial free fatty acids inhibit PrfA-dependent activation of virulence gene transcription by interfering with the DNA binding activity of PrfA. Our findings suggest that free fatty acids could be candidates for alternative therapies against L. monocytogenes.


1969 ◽  
Vol 26 (10) ◽  
pp. 2727-2736 ◽  
Author(s):  
Margaret L. Anderson ◽  
Elinor M. Ravesi

Freezing and holding cod muscle in the frozen state favored the association process that involves protein–free fatty acid (FFA) complex formation and begins during aging in ice. Changes in protein extractability, in ultracentrifugal patterns of protein extracted, and in phase contrast micrographs of inextractable muscle fragments were followed in muscle that had been aged in ice to produce various contents of FFA and then frozen and held at −29 C. After 11 months, these changes, which took place largely during the first week of storage, were comparable with those that occur when the FFA are formed during frozen storage. The results were consistent with a reaction rate that was greater at −29 C than at temperatures a few degrees above 0 C.


1997 ◽  
Vol 237 ◽  
pp. S25
Author(s):  
H. Heller ◽  
J. Deutsch ◽  
A.D. Purdon ◽  
S.I. Rapoport ◽  
M. Horowitz ◽  
...  

2020 ◽  
Vol 145 ◽  
pp. 104726
Author(s):  
Fabiano Gomes Ferreira de Paula ◽  
Rafael César Gonçalves Pereira ◽  
Breno Frederico Pereira Paulo ◽  
José Domingos Ardisson ◽  
Zenilda de Lourdes Cardeal ◽  
...  

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