Electrophoretic Variants of L-Alpha-Glycerophosphate Dehydrogenase in Pacific Ocean Perch (Sebastodes alutus)

1970 ◽  
Vol 27 (5) ◽  
pp. 943-945 ◽  
Author(s):  
A. G. Johnson ◽  
F. M. Utter ◽  
H. O. Hodgins
Keyword(s):  
Pacific Ocean ◽  
Genetic Variants ◽  
Gel Electrophoresis ◽  
Population Studies ◽  
Starch Gel Electrophoresis ◽  
Electrophoretic Variants ◽  
Glycerophosphate Dehydrogenase ◽  
Starch Gel

Three phenotypes of L-alpha-glycerophosphate dehydrogenase (α-GPDH) were found in muscle extracts of Pacific ocean perch by starch-gel electrophoresis. No association was obvious between alpha-glycerophosphate phenotype and sex or stage of maturity. The observed phenotypes were presumed to be under the control of two allelic genes, F and S. This assumption was supported by the distribution of phenotypes of fish collected off the coasts of Washington and Oregon. This report is the first on genetic variants of an enzyme in Pacific ocean perch and suggests that isozymes of α-GPDH may be useful in population studies of this species.

INHERITED VARIANTS OF α1-ANTITRYPSIN: A NEW ALLELE PiN

1974 ◽  
Vol 16 (2) ◽  
pp. 297-303 ◽  
Author(s):  
Diane Wilson Cox ◽  
Lynne Celhoffer
Keyword(s):  
Gel Electrophoresis ◽  
Null Allele ◽  
Starch Gel Electrophoresis ◽  
Electrophoretic Variants ◽  
Similar Region ◽  
New Variant ◽  
Starch Gel ◽  
Immunofixation Electrophoresis ◽  
Starch Gels ◽  
Antigen Antibody

A new inherited variant of α1-antitrypsin (protease inhibitor or Pi) has been found in five individuals of a family of Welsh origin. The new allele is called PiN, as the α1AT product migrates in acid starch gel between the products of the PiM and PiP alleles. The individuals carrying the PiN allele are all of Pi type MN. The new variant has been compared in several electrophoretic systems with other variants migrating in a similar region by acid starch gel electrophoresis (M, P, S, V, W and X). Acid starch gel and crossed antigen-antibody electrophoresis are most suitable for distinguishing the PiN product. By immunofixation electrophoresis, N has a mobility only slightly different from that of M, however the value of this method can be seen for distinguishing other slow variants which cannot be clearly distinguished on acid starch gels. Twenty-three variants of α1AT are now known. Twenty-two of these are electrophoretic variants and one, the null allele (Pi−), produces no α1AT.

Biochemical Genetics of Fundulus heteroclitus (L.): V. Inheritance of 10 Biochemical Loci

1988 ◽  
Vol 79 (5) ◽  
pp. 359-365 ◽  
Author(s):  
D. C. Brown ◽  
I. J. Ropson ◽  
D. A. Powers
Keyword(s):  
Gel Electrophoresis ◽  
Fundulus Heteroclitus ◽  
Tissue Specificity ◽  
Intracellular Localization ◽  
Natural Populations ◽  
Starch Gel Electrophoresis ◽  
Biochemical Genetics ◽  
Linkage Groups ◽  
Electrophoretic Variants ◽  
Starch Gel

Abstract Starch gel electrophoresis has shown that natural populations of Fundulus heteroclitus have electrophoretic variants for at least 21 loci. We provide inheritance data for 10 polymorphic systems: esterases (Est-B, EST-C, and Est-D); aspartate amlnotransferases (Aat-A, and Aat-B); mannosephosphate isomerase (Mpl-A); acid phosphatase (Ap-A); phosphoglucomutase (Pgm-B); hexose-6-phosphate dehydrogenase (H6pdh-A); and fumarase (Fum-A). Variants for nine of these loci segregate as autosomally inherited codominant alleles. The other system, EST-C, does not reflect such inheritance. We have identified two possible linkage groups: H6pdh-A may be loosely linked to Pgm-B, and Fum-A appears to be linked to Pgm-A. Tissue specificity and intracellular localization for all these loci are also presented.

INHERITANCE OF ELECTROPHORETIC VARIANTS OF SERUM ESTERASES IN DOMESTIC FOWL

1968 ◽  
Vol 10 (4) ◽  
pp. 961-967 ◽  
Author(s):  
A. A. Grunder
Keyword(s):  
Gel Electrophoresis ◽  
Esterase Activity ◽  
Domestic Fowl ◽  
Staining Intensity ◽  
Starch Gel Electrophoresis ◽  
Electrophoretic Variants ◽  
Region I ◽  
Starch Gel ◽  
The Mean

Three regions of serum esterase activity of chickens were differentiated by means of starch gel electrophoresis and appropriate stains. Regions I and II seemed to represent aliesterase while region III apparently represented a cholinesterase. The patterns of zones of region I were classified into three phenotypes named A, B, and AB and the hypothesis was proposed that these are controlled by two codominant alleles named EsA and AsB. The staining intensity of these esterase zones among adults was sex-associated in that the mean staining intensity of zones was greater in male than in female sera. Frequency of the EsB allele was 0.70, 0.76, and 0.85 in three meat lines.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

STARCH GEL ELECTROPHORESIS OF MYOGEN FROM CHICKEN BREAST MUSCLE IN CONSTANT AND GRADIENT BUFFER SYSTEMS

1963 ◽  
Vol 41 (1) ◽  
pp. 369-387 ◽  
Author(s):  
J. M. Neelin
Keyword(s):  
Gel Electrophoresis ◽  
Protein Concentration ◽  
Breast Muscle ◽  
Chicken Breast ◽  
Starch Gel Electrophoresis ◽  
Two Dimensional ◽  
Sodium Cacodylate ◽  
Gradient Systems ◽  
Optimal Separation ◽  
Starch Gel

By varying conditions of starch gel electrophoresis, factors contributing to the resolution of myogen proteins from chicken breast muscle have been studied. Variables examined included composition of the myogen extractant, protein concentration, ionic strength of electrophoretic media, pH of gel media, plane and direction of electrophoresis, and the nature of cations and anions in gel media and bridge solutions. The significance of anions was more closely studied with constant buffer systems, and gradient systems in which bridge electrolyte differed from, and gradually altered, the gel medium. Optimal separation was obtained in gradient systems with 0.10 M sodium chloride bridge solutions, and gel media of sodium cacodylate, pH 6.9, μ 0.010, which resolved 12 cationic zones, and sodium veronal, pH 7.4, μ 0.010, which resolved 10 anionic zones. These buffers in two-dimensional sequence revealed a total of about 24 components in this myogen.

Morphological and biochemical systematics of chubs, Nocomis biguttatus and N. micropogon (Pisces: Cyprinidae), in southern Ontario

1981 ◽  
Vol 59 (5) ◽  
pp. 771-775 ◽  
Author(s):  
Moira M. Ferguson ◽  
David L. G. Noakes ◽  
Roy G. Danzmann
Keyword(s):  
Gel Electrophoresis ◽  
Function Analysis ◽  
Morphological Differentiation ◽  
Sexually Dimorphic ◽  
Starch Gel Electrophoresis ◽  
Southern Ontario ◽  
Electrophoretic Mobilities ◽  
Starch Gel ◽  
Respective Species ◽  
Probability Of Misclassification

Examination of 17 presumptive gene loci by starch-gel electrophoresis revealed differential mobilities only at acid phosphatase-1, alcohol dehydrogenase, esterase-1, and phosphoglucomutase between Nocomis biguttatus and N. micropogon. No intraspecific variation was observed for any loci. The genetic identity (I) and genetic distance (D) were 0.874 and 0.134, respectively. The correlation of electrophoretic mobilities and nuptial tubercle pattern in sexually dimorphic males supports the present taxonomic distinction of these species and provides a simple, unambiguous means of identifying any individuals.Stepwise discriminant function analysis of a series of mensural characters was used to compare fish identified as to species by electrophoresis. At best this correctly assigned fish to their respective species in 85.7% of cases, with a probability of misclassification of 0.1335.This study suggests these two are sibling species, based on a comparison of biochemical and morphological differentiation.

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