Determining Cortisol and Cortisone Simultaneously in Salmonid Plasma by Competitive Protein Binding

1970 ◽  
Vol 27 (3) ◽  
pp. 596-601 ◽  
Author(s):  
U. H. M. Fagerlund
Keyword(s):  
Protein Binding ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Simultaneous Determination ◽  
Silica Gel ◽  
Chicken Serum ◽  
Competitive Protein Binding ◽  
Layer Chromatography

A method was developed for the simultaneous determination of nanogram quantities of cortisol and cortisone in 0.1-ml samples of salmonid plasma by competitive protein binding (CPB). The steroids were separated by thin-layer chromatography on silica gel and CPB analysis was performed with chicken serum in the presence of Florisil.

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