Lipolytic Activity Toward Long-Chain Triglycerides in Lateral Line Muscle of Rainbow Trout (Salmo gairdneri)

1969 ◽  
Vol 26 (7) ◽  
pp. 1857-1866 ◽  
Author(s):  
E. Bilinski ◽  
Y. C. Lau
Keyword(s):  
Rainbow Trout ◽  
Lateral Line ◽  
Lipolytic Activity ◽  
Salmo Gairdneri ◽  
Tissue Slices ◽  
Optimum Activity ◽  
Long Chain Triglycerides ◽  
Long Chain

Lipolytic activity toward long-chain triglycerides was studied in the lateral line muscle of rainbow trout (Salmo gairdneri) by use of tissue slices. Emulsification of substrates with phospholipids was found to be necessary to demonstrate lipolysis, which occurred in decreasing intensity with tripalmitin, triolein, and tristearin. The lipolytic activity showed optimum activity at pH 7.3 and it was inhibited by NaF, protamine, and p-chloromercuriphenyl sulfonate. Albumin had little effect on the activity. Epinephrine did not stimulate lipolysis, but it reduced loss of activity during preincubation.

Lysosomal Triglyceride Lipase from the Lateral Line Tissue of Rainbow Trout (Salmo gairdneri)

1971 ◽  
Vol 28 (7) ◽  
pp. 1015-1018 ◽  
Author(s):  
E. Bilinski ◽  
R. E. E. Jonas ◽  
Y. C. Lau
Keyword(s):  
Rainbow Trout ◽  
Lateral Line ◽  
Lysosomal Enzymes ◽  
Lipolytic Activity ◽  
Mitochondrial Fraction ◽  
Maximum Activity ◽  
Salmo Gairdneri ◽  
Acid Lipase ◽  
Triton X ◽  
Hydrolysis Of

An acid lipase active toward tripalmitin and having the characteristics of lysosomal enzymes was shown to occur in the red lateral line tissue of rainbow trout (Salmo gairdneri). The enzyme showed maximum activity at pH 4–4.5. Triton X-100 (0.2–2.0%) strongly stimulated the activity of the acid lipase, but it inhibited markedly the lipolytic activity above pH 7. NaF (20 mM) and Na-p-chloromercuriphenyl-sulfonate (1 mM) partially inhibited the acid lipase. Fractionation of the total homogenate by differential centrifugation in 0.25 M sucrose showed that the acid lipase was present at highest concentration in the light mitochondrial fraction. Palmitic acid and dipalmitin were the two main products of hydrolysis of tripalmitin.

Oxidation of Lactate to Carbon Dioxide by Rainbow Trout (Salmo gairdneri) Tissues

1972 ◽  
Vol 29 (10) ◽  
pp. 1467-1471 ◽  
Author(s):  
E. Bilinski ◽  
R. E. E. Jonas
Keyword(s):  
Rainbow Trout ◽  
White Muscle ◽  
Salmo Gairdneri ◽  
Oxidative Decarboxylation ◽  
Complete Oxidation ◽  
Exchange Reactions ◽  
Fish Tissues ◽  
Tissue Slices ◽  
Lactate Oxidation ◽  
Level Of Activity

A comparative study on the ability of various fish tissues to carry out different stages of lactate oxidation was conducted with rainbow trout (Salmo gairdneri). Rate of oxidation of Na-L-lactate-1-14C (5 mM) and Na-L-lactate-3-14C (5 mM) by tissue slices from white muscle, red lateral line muscle, heart, liver, kidney, and gills was determined at 15 C by measuring the formation of 14CO2. In all tissues the liberation of 14CO2 was considerably higher with lactate-1-14C than with lactate-3-14C. Liver was the most active tissue for oxidation of lactate-1-14C (2805 mμmoles/g wet tissue/hr at 15 C) and gills for oxidation of lactate-3-14C (556 mμmoles/g wet tissue/hr at 15 C). With both substrates activity in the white muscle was very limited, whereas other tissues had an intermediate level of activity. The results suggest that, in trout, the catabolism of lactate may take place through oxidative decarboxylation of pyruvate and that liver plays an important role in such a process. It appears also that complete oxidation of lactate may be of significance in supplying energy for the exchange reactions in gills.

Lipolytic Activity of Human Gastric and Duodenal Juice Against Medium and Long Chain Triglycerides

1971 ◽  
Vol 60 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Manley Cohen ◽  
Reginald G.H. Morgan ◽  
Alan F. Hofmann
Keyword(s):  
Lipolytic Activity ◽  
Duodenal Juice ◽  
Long Chain Triglycerides ◽  
Long Chain

Effects of Coenzyme A and Carnitine on Fatty Acid Oxidation by Rainbow Trout Mitochondria

1970 ◽  
Vol 27 (5) ◽  
pp. 857-864 ◽  
Author(s):  
E. Bilinski ◽  
R. E. E. Jonas
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Rainbow Trout ◽  
Fatty Acid Oxidation ◽  
Lateral Line ◽  
Coenzyme A ◽  
Maximal Activity ◽  
Salmo Gairdneri ◽  
Acid Oxidation ◽  
Mitochondrial Fractions

The effects of coenzyme A (CoA) and carnitine on the oxidation of 0.1 mM K-palmitate-1-14C and K-oleate-1-14C by mitochondrial fractions from lateral line muscle, white muscle, heart, liver, and kidney was studied in rainbow trout (Salmo gairdneri). CoA (0.01–0.10 mM) stimulated the oxidation of fatty acids by mitochondria from lateral line muscle and heart. With all the preparations of mitochondria, fatty acid oxidation was increased by addition of carnitine (1.0 mM) and in the presence of carnitine (1.0 mM), CoA (0.1–1.0 mM) gave a further increase in oxidative activity. Mitochondria from lateral line muscle and heart showed the same range of maximal activity (oxidized substrate at 15 C = 180–200 nmoles/mg N per hr), which was considerably above that found with mitochondria from other tissues. The results suggest similarities in the energy-supplying metabolism in lateral line muscle and heart of trout with respect to the utilization of fatty acids.

Postprandial Activation of Coagulant Factor VII by Long-chain Dietary Fatty Acids

1996 ◽  
Vol 76 (03) ◽  
pp. 369-371 ◽  
Author(s):  
T A B Sanders ◽  
G J Miller ◽  
Tamara de Grassi ◽  
Najat Yahia
Keyword(s):  
Fatty Acids ◽  
Dietary Fatty Acids ◽  
Factor Vii ◽  
Fat Intake ◽  
Long Chain Fatty Acids ◽  
Postprandial Lipaemia ◽  
Increased Risk ◽  
Coagulant Activity ◽  
Long Chain Triglycerides ◽  
Long Chain

SummaryFactor VII coagulant activity (FVIIc) is associated with an increased risk of fatal ischaemic heart disease (IHD). Several reports have suggested that dietary fat intake or hypertriglyceridaemia are associated with elevated levels of FVII. This study demonstrates that an intake of long-chain fatty acids sufficient to induce postprandial lipaemia in healthy subjects leads to a substantial elevation in both FVIIc and the concentration of FVII circulating in the activated form. Such an increase in FVIIc could not be induced by medium-chain triglycerides. These results suggest that the consumption of a sufficient amount of long-chain triglycerides to induce postprandial lipaemia induces the activation of FVII.

Temperature Selection of Rainbow Trout (Salmo gairdneri) Fingerlings in Vertical and Horizontal Gradients

1971 ◽  
Vol 28 (11) ◽  
pp. 1801-1804 ◽  
Author(s):  
R. W. McCauley ◽  
W. L. Pond
Keyword(s):  
Rainbow Trout ◽  
Salmo Gairdneri ◽  
Temperature Preference ◽  
Temperature Selection ◽  
Horizontal Gradients ◽  
Significant Difference ◽  
Preferred Temperatures ◽  
Laboratory Investigations ◽  
Horizontal Temperature Gradients ◽  
Selection Of

Preferred temperatures of underyearling rainbow trout (Salmo gairdneri) were determined in both vertical and horizontal temperature gradients. No statistically significant difference was found between the preferred temperatures by the two different methods. This suggests that the nature of the gradient plays a lesser role than generally believed in laboratory investigations of temperature preference.

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