Isolation and Preliminary Characterization of Some Aeromonas salmonicida Bacteriophages

1969 ◽  
Vol 26 (3) ◽  
pp. 629-632 ◽  
Author(s):  
W. D. Paterson ◽  
R. J. Douglas ◽  
I. Grinyer ◽  
L. A. McDermott
Keyword(s):  
Aeromonas Salmonicida ◽  
Southern Ontario ◽  
Similar Morphology ◽  
Group Iii ◽  
Preliminary Characterization ◽  
Group I ◽  
Tail Sheath ◽  
Contractile Tail

Bacteriophages for Aeromonas salmonicida were isolated from water and mud samples taken from 13 of 19 trout hatcheries and ponds examined in southern Ontario. Nine of the 13 locations were known to have histories of furunculosis disease. The bacteriophages studied formed three serological groups and two distinct morphological types. Phages of serological groups I and II possessed similar morphology; those of group III resembled the coli T-even phages. All possessed complex symmetry consisting of a head and tail with a contractile tail sheath. Latent periods of the phages ranged from 35 to 95 min and average burst sizes from 21 to 193. A group I phage and a group III phage had [Formula: see text] values of 351 and 1000, respectively.

Evaluation of the level of natriuretic peptides in the diagnosis and characterization of chronic kidney disease and cardiorenal syndrome in children

2021 ◽  
Vol 25 (6) ◽  
pp. 87-92
Author(s):  
A. M. Mambetova ◽  
D. V. Bizheva ◽  
I. K. Thabisimova
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Natriuretic Peptides ◽  
Cardiorenal Syndrome ◽  
Stage I ◽  
Group Iii ◽  
Early Stages ◽  
Group I ◽  
Ckd Stage

BACKGROUND. Natriuretic peptides have cardio- and renoprotective effects, inhibiting inflammatory and proliferative processes. The role of natriuretic peptides in the early diagnosis and characterization of chronic kidney disease (CKD) and cardiovascular complications as the disease development and progresses has not been studied.TNEAIM: to study the level of natriuretic peptides in children depending on the stage of CKD development and to assess the significance of this indicator.PATIENTS AND METHODS. The study involved 93 children with congenital diseases of the urinary system at the age from 3 to 18 years. Three groups were identified: group I - 54 patients with CKD stage I , group II - 29 patients with CKD stage II; Group III - 10 children with CKD stages IV-V (patients with CKD stages IV and V were combined due to their small amount). Control group - 10 clinically healthy children of the corresponding age. The N-terminal propeptide of natriuretic hormone (NT-proBNP) was determined in the blood by the enzyme-linked immunosorbent assay.RESULTS. An increase in the level of NT-proBNP by 28.7% takes place already in the early stages of CKD. With the progression of CKD, an increase in the level of NT-proBNP was noted from 57.4 % in children in the group of patients with stage I CKD to 80 % in children in group III patients. The maximum concentrations of NT-proBNP, many times higher than those in CKD stages I and II, were observed in children with CKD stages IV-V. The degree of increase in the level of NT-proBNP correlated with the severity of CKD.CONCLUSION. In the diagnosis and characterization of CKD and cardiorenal syndrome in children, the determination of the level of natriuretic peptides is of great importance. A high level of natriuretic peptides characterizes the presence of cardiorenal relationships and can be used as an additional criterion for assessing the severity of CKD, including at the early stages of its development.

scholarly journals Isolation and characterization of β- and γ-caseins from horse milk

1982 ◽  
Vol 203 (1) ◽  
pp. 131-139 ◽  
Author(s):  
S Visser ◽  
R Jenness ◽  
R J Mullin
Keyword(s):  
Room Temperature ◽  
Sodium Dodecyl ◽  
Sulphate Group ◽  
Group Iii ◽  
Isolation And Characterization ◽  
Group I ◽  
Beta Casein ◽  
Group Ii ◽  
Phosphate Groups

Three groups of casein components were isolated from horse milk. Group I is almost insoluble at acid and neutral pH, and is rather heterogeneous on alkaline gels with or without sodium dodecyl sulphate. Group II shows strong similarity to beta-casein from other species, as concluded from its amino acid composition and its N- and C-terminal sequences. This group consists of five electrophoretically distinguishable forms, all containing ester phosphate groups but no carbohydrate. Group III is composed of C-terminal fragments of the beta-like (group II) fraction and probably arises from the action of a plasmin-like enzyme present in horse milk. It does not contain phosphate or carbohydrate. Homology of this group with bovine gamma-caseins is demonstrated. Both beta- and gamma-like caseins are more soluble at 4 degrees C than at room temperature.

scholarly journals Interstrain Inhibition in the Sweet Potato Pathogen Streptomyces ipomoeae: Purification and Characterization of a Highly Specific Bacteriocin and Cloning of Its Structural Gene

2003 ◽  
Vol 69 (4) ◽  
pp. 2201-2208 ◽  
Author(s):  
Xiujun Zhang ◽  
Christopher A. Clark ◽  
Gregg S. Pettis
Keyword(s):  
Sweet Potato ◽  
Structural Gene ◽  
Gene Sequence ◽  
Purification And Characterization ◽  
Cationic Protein ◽  
Group Iii ◽  
Inhibitory Function ◽  
Group I ◽  
Made In

ABSTRACT Strains of the sweet potato soil rot pathogen Streptomyces ipomoeae had previously been divided into three groups based on their ability to inhibit one another during pairwise cocultivation. While group I strains are not antagonistic to members of the other groups, group II and group III strains produce separate substances that are inhibitory to strains outside their respective cognate groups. Here, we purified the group III inhibitory substance from the culture supernatant of a representative strain and found that it consists of a single 10-kDa cationic protein which is bacteriolytic for S. ipomoeae group I and II strains but which showed no inhibitory function against other streptomycetes or other bacterial genera tested. The structural gene for the inhibitor was cloned from a chromosomal library of the producing strain, and while the gene sequence revealed that the inhibitor is initially made in a larger precursor form, the deduced mature protein showed no significant homology to other known proteins. Our results demonstrate that S. ipomoeae group III inhibitory activity is manifested in the form of a highly specific, potentially novel bacteriocin, which we have designated ipomicin.

scholarly journals Morphological and Molecular characterization of rhizoctonia oryzae sativae in Bangladesh

2019 ◽  
Vol 16 (2) ◽  
pp. 119-128
Author(s):  
SB Jahan ◽  
MA Ali ◽  
MS Alam ◽  
ZR Moni ◽  
MA Latif
Keyword(s):  
Molecular Characterization ◽  
Group Iii ◽  
Cluster Group ◽  
Group I ◽  
Rhizoctonia Oryzae ◽  
Geographic Origins ◽  
Slow Growing ◽  
North Western ◽  
Morphological And Molecular Characterization

Aggregate sheath spot disease of rice caused by Rhizoctonia oryzaesativae has emerged in higher incidence in North-Western region of Bangladesh. Thirty isolates of R. oryzae-sativae were studied by using morphological and molecular marker. Isolates were confirmed using specific primer where a single band of 1200bp was amplified. Two distinct groups relatively slow and faster were found in mycelal growth. Molecular characterization was done using four primers and DNA band ranged from 0.25 to 2.21 kb. A combined dendrogram was constructed which separated the isolates into three groups at 69.6% similarity level. All isolates placed in two major clusters except isolate RA-1 placed in cluster group III but were not grouped according to their geographic origins. Fast growing isolates have been placed in Group II while slow growing isolates in cluster group I. The similarity coefficient values of the dendrogram profile ranged from 0.36 to 0.98 with an average of 0.67. Diversity of different isolate showed that significant variation was present among the isolate and were not genetically identical. SAARC J. Agri., 16(2): 119-128 (2018)

Electrochemistry and spectral characterization of arsenic porphyrins with σ-bonded axial ligands: X-ray crystallographic analysis of [(OEP)As(F)2]+PF6−, [(OEP)As(CH3)(OCH3)]+ClO4− and [(OEP)As(C2H5)2]+PF6−

2002 ◽  
Vol 06 (05) ◽  
pp. 325-335 ◽  
Author(s):  
Karl M. Kadish ◽  
Zhongping Ou ◽  
Xiaoyu Tan ◽  
Wataru Satoh ◽  
Yohsuke Yamamoto ◽  
...  
Keyword(s):  
Esr Spectroscopy ◽  
Supporting Electrolyte ◽  
Crystallographic Analysis ◽  
Reduced Form ◽  
X Ray ◽  
Group Iii ◽  
Axial Ligands ◽  
Group I ◽  
Uv Visible

The electrochemistry of nine arsenic(V) octaethylporphyrins is reported in benzonitrile or dichloromethane containing 0.1 M tetra-n-butylammonium perchlorate as supporting electrolyte and the results compared to data for phosphorus and antimony porphyrins containing a similar set of σ-bonded and/or anionic axial ligands. The investigated compounds are divided into three groups based on the nature of the axial ligands and are represented as [( OEP ) As ( R )( R ')]+ (group I), [( OEP ) As ( R )( X )]+ (group II) and [( OEP ) As ( F )2]+ (group III) where R and R’ = CH 3 or C 2 H 5, X = OH −, OCH 3−, OC 2 H 5−, OC 3 H 7− or NHC 4 H 9− and OEP = the dianion of octaethylporphyrin. Each compound was characterized in its neutral, oxidized and reduced form by UV-visible and ESR spectroscopy. An X-ray crystallographic analysis of [( OEP ) As ( F )2]+ PF 6−, [( OEP ) As ( CH 3)( OCH 3)]+ ClO 4− and [( OEP ) As ( C 2 H 5)2]+ PF 6− is also presented.

scholarly journals Heterologous antigen extract in ELISA for the detection of human IgE anti-Strongyloides stercoralis

2003 ◽  
Vol 45 (5) ◽  
pp. 265-268 ◽  
Author(s):  
Julia Maria Costa-Cruz ◽  
Joaquina Madalena ◽  
Deise Aparecida de Oliveira Silva ◽  
Mônica Camargo Sopelete ◽  
Dulcinéa Maria Barbosa Campos ◽  
...  
Keyword(s):  
Intestinal Parasites ◽  
Strongyloides Stercoralis ◽  
Specific Ige ◽  
Serum Samples ◽  
Heterologous Antigen ◽  
Total Ige ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Strongyloides ratti larval extract was used for the standardization of ELISA to detect genus-specific IgE in human strongyloidiasis. Forty serum samples from monoinfected patients shedding S. stercoralis larvae (Group I), 40 from patients with other intestinal parasites (Group II), and 40 from copronegative healthy subjects (Group III) were analyzed. Genus-specific IgE levels (ELISA Index: EI) were significantly higher in the group I (EI = 1.43) than groups II (EI = 0.70) and III (EI = 0.71), showing positivity rates of 55%, 2.5% and 0%, respectively. Similarly, sera from copropositive patients had significantly higher levels of total IgE (866 IU/mL) as compared to those from group II (302 IU/mL) and III (143 IU/mL). A significant positive correlation was found between levels of Strongyloides specific-IgE and total IgE in sera from patients with strongyloidiasis. In conclusion, S. ratti heterologous extract showed to be a useful tool for detecting genus-specific IgE by ELISA, contributing for a better characterization of the immune response profile in human strongyloidiasis.

scholarly journals Purification and Biochemical Characterization of Mutacin I from the Group I Strain of Streptococcus mutans, CH43, and Genetic Analysis of Mutacin I Biosynthesis Genes

2000 ◽  
Vol 66 (8) ◽  
pp. 3221-3229 ◽  
Author(s):  
Fengxia Qi ◽  
Ping Chen ◽  
Page W. Caufield
Keyword(s):  
Genetic Analysis ◽  
Streptococcus Mutans ◽  
Structural Gene ◽  
Biochemical Characterization ◽  
Peptide Sequencing ◽  
Protein Sequence Analysis ◽  
Group Iii ◽  
Isolation And Characterization ◽  
Group I

ABSTRACT Previously, we reported isolation and characterization of mutacin III and genetic analysis of mutacin III biosynthesis genes from the group III strain of Streptococcus mutans, UA787 (F. Qi, P. Chen, and P. W. Caufield, Appl. Environ. Microbiol. 65:3880–3887, 1999). During the same process of isolating the mutacin III structural gene, we also cloned the structural gene for mutacin I. In this report, we present purification and biochemical characterization of mutacin I from the group I strain CH43 and compare mutacin I and mutacin III biosynthesis genes. The mutacin I biosynthesis gene locus consists of 14 genes in the order mutR, -A, -A′, -B, -C, -D, -P, -T, -F, -E, -G, orfX, orfY, orfZ. mutA is the structural gene for mutacin I, whilemutA′ is not required for mutacin I activity. DNA and protein sequence analysis revealed that mutacins I and III are homologous to each other, possibly arising from a common ancestor. The mature mutacin I is 24 amino acids in size and has a molecular mass of 2,364 Da. Ethanethiol modification and peptide sequencing of mutacin I revealed that it contains six dehydrated serines, four of which are probably involved with thioether bridge formation. Comparison of the primary sequence of mutacin I with that of mutacin III and epidermin suggests that mutacin I likely has the same bridging pattern as epidermin.

scholarly journals Geochemical Characterization of Zircon in Fyfe Hills of the Napier Complex, East Antarctica

Minerals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 943
Author(s):  
Mami Takehara ◽  
Kenji Horie ◽  
Tomokazu Hokada
Keyword(s):  
High Temperature ◽  
East Antarctica ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Uht Metamorphism ◽  
Ree Patterns ◽  
High Temperature Metamorphism ◽  
Napier Complex

Ultra-high temperature (UHT) metamorphism plays an essential role in the development and stabilization of continents through accretionary and collisional orogenesis. The Napier Complex, East Antarctica, preserves UHT metamorphism, and the timing is still debated. U–Pb zircon geochronology integrated with rare earth element (REE) and oxygen isotope was applied to a garnet-bearing quartzo-feldspathic gneiss to confirm the timing of UHT metamorphism in Fyfe Hills in the western part of the Napier Complex. The zircons are analyzed using a sensitive high-resolution ion microprobe (SHRIMP). The cathodoluminescence observation and U–Pb ages allowed us to classify the analytical domains into three types: inherited domains (Group I), metamorphic domains (Group II), and U–Pb system disturbed domains (Group III). The REE patterns of Group II are characterized by a weak fractionation between the middle REE and heavy REE, which reinforces the above classification. The 207Pb*/206Pb* ages of Group II have an age peak at 2501 Ma, therefore, the gneiss experienced high temperature metamorphism at 2501 Ma. δ18O of zircons are homogeneous among the three groups (5.53 ± 0.11‰, 5.51 ± 0.14‰, and 5.53 ± 0.23‰), which suggests re-equilibration of oxygen isotopes after metamorphism at ca. 2501Ma under dry UHT conditions.

scholarly journals Purification and characterization of three distinct types of phospholipase A2 inhibitors from the blood plasma of the Chinese mamushi, Agkistrodon blomhoffii siniticus

1997 ◽  
Vol 325 (2) ◽  
pp. 527-531 ◽  
Author(s):  
Naoki OHKURA ◽  
Hiroaki OKUHARA ◽  
Seiji INOUE ◽  
Kiyoshi IKEDA ◽  
Kyozo HAYASHI
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Phospholipase A2 ◽  
Blood Plasma ◽  
Amino Acid Residues ◽  
Group Iii ◽  
Group I ◽  
Binding Surface ◽  
Group Ii

Three distinct types of phospholipase A2 (PLA2) inhibitory proteins (PLIα, PLIβ, and PLIγ) were isolated from the blood plasma of the Chinese mamushi, Agkistrodonblomhoffiisiniticus. PLIα is an inhibitor that we have already purified and whose amino acid sequence we have already determined [Ohkura, Inoue, Ikeda and Hayashi (1993) J. Biochem. (Tokyo) 113, 413–419]. It inhibited selectively the group-II acidic PLA2s from Crotalidae venom. PLIβ was a 160-kDa glycoprotein having a trimeric structure composed of 50-kDa subunits. The amino acid sequence of the first 30 amino acids of the N-terminal part of the 50-kDa subunit was determined and found to have no significant homology to that of known proteins. PLIβ was a selective inhibitor against the group-II basic PLA2s from Crotalidae venom. Some amino acid residues located in or close to the interfacial binding surface of the group-II basic PLA2s were suggested to be involved in selective binding to PLIβ. PLIγ was a 100-kDa glycoprotein containing 25-kDa and 20-kDa subunits and inhibited all of the PLA2s investigated equally, including Elapidae venom PLA2s (group I), Crotalidae and Viperidae venom PLA2s (group II) and honey-bee PLA2 (group III). From the N-terminal sequences of the two subunits, PLIγ was found to be the same type of PLI that had been purified from Thailand cobra plasma.

Ultrastructural Lesions Produced by Alcohol and Sucrose in the Heart and Liver of C57BL Mice

1970 ◽  
Vol 28 ◽  
pp. 208-209
Author(s):  
K.K. SEKHRI ◽  
C.S. ALEXANDER ◽  
H.T. NAGASAWA
Keyword(s):  
Osmium Tetroxide ◽  
Male Mice ◽  
Alcohol Group ◽  
Group Iii ◽  
Group I ◽  
C57bl Mice ◽  
Group Ii

C57BL male mice (Jackson Lab., Bar Harbor, Maine) weighing about 18 gms were randomly divided into three groups: group I was fed sweetened liquid alcohol diet (modified Schenkl) in which 36% of the calories were derived from alcohol; group II was maintained on a similar diet but alcohol was isocalorically substituted by sucrose; group III was fed regular mouse chow ad lib for five months. Liver and heart tissues were fixed in 2.5% cacodylate buffered glutaraldehyde, post-fixed in 2% osmium tetroxide and embedded in Epon-araldite.

Sign in / Sign up

Export Citation Format

Share Document