Lactic Acidosis in Channel Catfish

1968 ◽  
Vol 25 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Charles W. Caillouet Jr.
Keyword(s):  
Lactic Acid ◽  
Lactic Acidosis ◽  
Respiratory Rate ◽  
Acid Concentration ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Lactic Acid Concentration ◽  
Blood Ph

Channel catfish (Ictalurus punctatus) were subjected to 5 or 10 min of anoxia (removed from water) to rapidly increase blood lactic acid concentration metabolically. Blood pH decreased as blood lactic acid concentration increased. Respiratory rate increased in fish with lowered blood pH and with increased blood lactic acid concentration during the 10 min after fish were returned to water after anoxia. Fish not treated had a mean blood pH of 7.8. Blood pH decreased in catfish subjected to anoxia 5, 10, and 15 min and decreased further when the fish were returned to water. Lowered blood pH was associated with loss of equilibrium and death in catfish subjected to transportation and handling.

Lactic acidosis and its effect on wool tensile strength

1992 ◽  
Vol 43 (8) ◽  
pp. 1747 ◽  
Author(s):  
PJ Murray ◽  
JB Rowe ◽  
EJ Speijers
Keyword(s):  
Tensile Strength ◽  
Lactic Acid ◽  
Lactic Acidosis ◽  
Acid Concentration ◽  
Basal Diet ◽  
Feed Additive ◽  
Lactic Acid Concentration ◽  
Wheat Grain ◽  
Rumen Ph ◽  
Staple Strength

An experiment was conducted to test the effect of the feed additive virginiamycin (VM) on lactic acidosis and wool tensile strength in sheep given wheat. The ground wheat grain was given in a slurry by stomach tube in increasing amounts each day up to a maximum of 1 kg per dose or until rumen pH dropped below 5.20. Sixty mature Merino wethers were all fed wheat chaff ad libitum and given the following treatments: 10 control sheep were only fed the basal diet; 10 were given intra-ruminal doses of VM; 20 sheep were given wheat grain intra-ruminally; and 20 sheep were given wheat grain containing VM. Virginiarnycin was given at a rate equivalent to 80 mg/day. All animals were fed chaff for 83 days prior to and for 120 days following the wheat and VM treatments. Rumen pH and L-lactic acid concentration were measured in all 60 sheep from the day that wheat was first given until 3 days after the last wheat drench. Lactic acidosis, defined by a rumen pH of less than 5.2 and rumen lactic acid concentration of greater than 20 mmol/L, significantly decreased staple strength of wool in sheep given the wheat drenches. Inclusion of VM with the wheat grain significantly increased (P < 0.05) the tensile strength of the wool compared with the effect of wheat on its own. Reduction in wool strength was more closely correlated with a reduction in feed intake in the days following the lactic acidosis than with either rumen pH or the concentration of rumen lactic acid.

The acid-base responses of gills and kidneys to infused acid and base loads in the channel catfish, Ictalurus punctatus

1982 ◽  
Vol 99 (1) ◽  
pp. 143-160
Author(s):  
J. N. Cameron ◽  
G. A. Kormanik
Keyword(s):  
Lactic Acid ◽  
Intracellular Ph ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Tissue Damage ◽  
Tissue Necrosis ◽  
Acid Base ◽  
Blood Ph ◽  
Extracellular Compartment ◽  
Acid And Base

Acid and base loads infused into the dorsal aorta of the freshwater channel catfish (Ictalurus punctatus Rafinesque) were excreted by both gills and kidneys. The gills excreted 2-3 times as much as the kidneys. After a 2 mmol. kg-1 load of NaHCO3, the gills excreted 50% of the net OH- load in the first 5 h, while the kidneys excreted 18% in the first 6 h. After a 2 mmol. kg-1 load of NH4Cl, the gills excreted 32% of the load in 6 h, and 16% was excreted renally in 20 h. There was no evidence of tissue damage after either NaHCO3 or NH4Cl infusions, whereas infusion of 2 mmol. kg-1 HCl or 1 mmol. kg-1 of L(+)-lactic acid caused significant kidney damage and extensive tissue necrosis within 24 h. After both NaHCO3 and NH4Cl infusions, the majority of the load had been transferred to the intracellular compartment within 2 h. From there it was excreted slowly, presumably by transfer back through the extracellular compartment. Due to the relative compartment volumes and buffer values, the change in intracellular pH was less than 0.05 units, while the blood pH was changed by as much as 0.3 units.

Post-Mortem Diagnosis of Hypoxia by Means of Brain Lactic Acid Concentration

1958 ◽  
Vol 192 (3) ◽  
pp. 577-580 ◽  
Author(s):  
Donald D. Van Fossan ◽  
Robert T. Clark
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Oxygen Deficiency ◽  
Postmortem Brain ◽  
Post Mortem ◽  
Lactic Acid Concentration ◽  
Altitude Exposure ◽  
Post Mortem Brain ◽  
Exposure Levels ◽  
The Brain

Simulated altitude exposure elevates the postmortem brain lactic acid concentration up to 98 mg/100 gm above controls depending on species used, duration, and intensity of exposure. The sharp difference in post-mortem brain lactic acid concentration between altitude exposed animals and controls remains demonstrable for the longest postmortem intervals studied (20 hr. in the dog, 30 hr. in the rabbit, and 6 hr. in the rat). Upon recovery from altitude exposure the brain lactic acid and/or precursors return toward pre-exposure levels in accordance with first order reaction kinetics during the first few minutes. The velocity constant is .32 and the half-life is 2.2 minutes. Elevated post-mortem brain lactic acid concentration is a constant finding in animals which were hypoxic at the time of death and appears to be a suitable criterion for establishing ante-mortem altitude exposure or other physiologically similar oxygen deficiency situations.

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