The Ultrastructure of the White Striated Myotomal Muscle of the Cod, Gadus morhua

1967 ◽  
Vol 24 (12) ◽  
pp. 2549-2553 ◽  
Author(s):  
C. M. Bishop ◽  
P. H. Odense

The structure of the white skeletal muscle of the cod (Gadus morhua) is described. The peripheral fibrils are ribbon-like and rectangular in cross section with the long axis normal to the sarcolemma. The inner fibrils are mainly polygonal in cross section. Most of the mitochondria and nuclei are peripheral to the fibrils and next to the sarcolemma. The arrangement of the contractile proteins is typical for striated muscle, and the sarcoplasmic reticulum and transverse tubular system are similar to those in other white skeletal fish muscle. A distinct N-band is apparent with indications of branching and reorientation of the actin filaments. Mitochondria are often closely associated with the Z line.

1991 ◽  
Vol 24 (1) ◽  
pp. 1-73 ◽  
Author(s):  
Christopher C. Ashley ◽  
Ian P. Mulligan ◽  
Trevor J. Lea

It has been known for a number of years that calcium ions play a crucial role in excitation-contraction (e-c) coupling (Sandow, 1952). The majority of the calcium required for this process is derived, at least in vertebrate striated muscle fibres, from discrete intracellular stores located at sites within the cell: the terminal cysternae (tc)/junctional SR of the sarcoplasmic reticulum (SR) (Fig. 1 a). These storage sites not only form a compartment that is distinct from the sarcoplasm of the fibre, but they are also closely associated with the contractile elements, the myofibrils. The SR release sites are activated following the spread of electrical activity (Huxley and Taylor, 1958) along the transverse (T) tubular system (Eisenberg and Gage, 1967; Adrian et al. 1969a, b; Peachey, 1973) from the surface membrane (Bm).


1968 ◽  
Vol 25 (7) ◽  
pp. 1339-1345 ◽  
Author(s):  
C. M. Morrison ◽  
P. H. Odense

The striated adductor muscle of the scallop Placopecten magellanicus is described. The fusiform uninucleate cells contain one fibril which is divided into A and I zones, and has Z and H bands. The A zone is 1.9 μ long, and in this zone apart from the H band about eight actin filaments surround each myosin filament. A sarcoplasmic reticulum envelops the fibril. Tubules of this reticulum are mainly oriented longitudinally and swellings from dyads with the sarcolemma.


Certain advances due mainly to H. E. Huxley (see Huxley 1961, 1963) have made it possible to use the electron microscope to study the detailed structure of the filaments in the contractile apparatus. The results of our work on actin filaments have already been published (Hanson & Lowy 1962, 1963). We shall now examine some of the consequences of these findings, including certain unsolved problems which they raise. Actin in the polymerized form ( F -actin) has been prepared from rabbit skeletal muscle by the usual methods and examined in negatively stained preparations in the electron microscope (Hanson & Lowy 1963). It has been found that solutions of F -actin are, in fact, suspensions of filaments. These consist of globular subunits arranged in a characteristic helical manner (figure 15).


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