The Vitamin B12 Content of Certain Fishery Materials

1953 ◽  
Vol 10 (2) ◽  
pp. 64-68 ◽  
Author(s):  
B. A. Southcott ◽  
H. L. A. Tarr
Keyword(s):  
Vitamin B12 ◽  
Chromatographic Separation ◽  
Growth Response ◽  
Microbiological Assay ◽  
Vitamin B ◽  
Hexane Extraction ◽  
Clupea Pallasi ◽  
Assay Procedure ◽  
Lactobacillus Leichmannii

Irregularities occurring in the microbiological assay of herring (Clupea pallasi) materials for vitamin B12 (cobalamin) are described and the probable reasons for the differences discussed. With the employment of a Lactobacillus leichmannii assay procedure and conditions under which vitamin B12 (cyano-cobalamin) and vitamin B13a (hydroxo-cobalamin) caused identical growth response, it was found that normal commercial flame-dried herring meals had about the same total cobalamin content as specially prepared meals dried at 38° to 43.5 °C. Chromatographic separation, elution and assay of the cobalamins in herring-meal samples indicated that substantially all of the activity found by direct assays of the meals was due to the vitamin itself. Hexane extraction had little effect on the cobalamin content, but heating the meals reduced it.

THE CONSTITUENTS OF COD LIVER WITH VITAMIN B12 ACTIVITY FOR LACTOBACILLUS LEICHMANNII

1956 ◽  
Vol 34 (2) ◽  
pp. 191-196
Author(s):  
Beryl Truscott ◽  
P. L. Hoogland
Keyword(s):  
Ion Exchange ◽  
Vitamin B12 ◽  
Microbiological Assay ◽  
Countercurrent Distribution ◽  
Vitamin B ◽  
Partition Chromatography ◽  
Total Vitamin ◽  
Beef Liver ◽  
Fractionation Procedure ◽  
Lactobacillus Leichmannii

The identity of the compounds that together form the total microbiological vitamin B12 activity of cod-liver residue was determined by a fractionation procedure involving ion exchange, countercurrent distribution, partition chromatography, and microbiological assay. The presence of cyanocobalamin, hydroxocobalamin, and the desoxyribosides of thymine, uracil, hypoxanthine, xanthine, and guanine was demonstrated. The total vitamin B12 activity, as determined by microbiological assay with Lactobacillus leichmannii, and its distribution in cod-liver residue, were very similar to those of beef liver. Cod-liver residues may be used to replace beef liver in vitamin B12 preparations.

THE CONSTITUENTS OF COD LIVER WITH VITAMIN B12 ACTIVITY FOR LACTOBACILLUS LEICHMANNII

1956 ◽  
Vol 34 (1) ◽  
pp. 191-196
Author(s):  
Beryl Truscott ◽  
P. L. Hoogland
Keyword(s):  
Ion Exchange ◽  
Vitamin B12 ◽  
Microbiological Assay ◽  
Countercurrent Distribution ◽  
Vitamin B ◽  
Partition Chromatography ◽  
Total Vitamin ◽  
Beef Liver ◽  
Fractionation Procedure ◽  
Lactobacillus Leichmannii

The identity of the compounds that together form the total microbiological vitamin B12 activity of cod-liver residue was determined by a fractionation procedure involving ion exchange, countercurrent distribution, partition chromatography, and microbiological assay. The presence of cyanocobalamin, hydroxocobalamin, and the desoxyribosides of thymine, uracil, hypoxanthine, xanthine, and guanine was demonstrated. The total vitamin B12 activity, as determined by microbiological assay with Lactobacillus leichmannii, and its distribution in cod-liver residue, were very similar to those of beef liver. Cod-liver residues may be used to replace beef liver in vitamin B12 preparations.

Commercially-supplied binders for plasma cobalamin (vitamin B12), analysis--"purified" intrinsic factor, “cobinamide"-blocked R-protein binder, and non-purified intrinsic factor-R-protein binder--compared to microbiological assay.

1980 ◽  
Vol 26 (5) ◽  
pp. 598-600 ◽  
Author(s):  
N P Kubasik ◽  
M Ricotta ◽  
H E Sine
Keyword(s):  
Protein Binding ◽  
Vitamin B12 ◽  
Intrinsic Factor ◽  
Microbiological Assay ◽  
Binding Agents ◽  
Lactobacillus Leichmannii

Abstract Recent evidence (Kolhouse et al., N. Engl. J. Med. 299: 785–792, 1978) demonstrates that commercial cobalamin (Vitamin B12) radioassay kits contain nonspecific R-protein binding agents that can give falsely normal results in patients who are actually cobalamin deficient. We tested three kits: with “purified” intrinsic factor as the binder, with intrinsic factor and the nonspecific R-protein sites blocked with “cobinamide,” and non-purified intrinsic factor-R-protein binder. Results with use of the first two compared well with those by a microbiological assay (Lactobacillus leichmannii) and are in harmony with clinical impressions.

Beziehungen zwischen Vitamin B12, Purinen und Pyrimidinen bei Lactobacillus leichmannii 313

1951 ◽  
Vol 6 (1) ◽  
pp. 25-38 ◽  
Author(s):  
Friedrich Weygand ◽  
Adolf Wacker ◽  
Friedrich Wirth
Keyword(s):  
Vitamin B12 ◽  
Vitamin B ◽  
Lactobacillus Leichmannii

Abstract Es wurde das Purin-und Pyrimidinbediirfnis von Lactobacillus leichmannii 313 ermittelt. Dabei ergab sich, daß lediglich Guanin und Uracil für ein optimales Wachstum erforderlich sind. Adenin hemmt in Abwesenheit von Guanin oder Xanthin in physiologischer Konzentration das durch Vitamin B10 oder die Desoxyriboside (mit Ausnahme von Adenindesoxyribosid) hervor-gerufene Wachstum. Es wird gefolgert, daß Lb. leichmannii 313 nicht in der Lage ist, Adenin in Guanin zu verwandeln. Von einer größeren Zahl von Pyrimidinen und Benzimidazolen, die in Abwesenheit von Adenin als Hemmstoffe des Bakterienwachstums geprüft wurden, erwiesen sich 4-Amino-uracil, 4-Amino-2-thio-uracil, 5-Methyl-4-amino-2-thio-uracil, Benzimidazol, Dimethyl-, Dibrom-und Dichlorbenzimidazol als wirksam. Die durdi diese Verbindungen hervorgerufene Wachstums-hemmung von Lb. leichmannii 313 konnte kompetitiv durch Adenin oder davon sich ableitende Verbindungen, wie Adenosin und Hefeadenylsäure, nichtkompetitiv durch Vitamin B^, auf-gehoben werden. 4-Amino-uracil und 5-Methyl-4-amino-2-thio-uracil zeigten beim Mäuse-Ascitestumor eine Verzögerung der Ascitesbildung. Die Darstellung der bei den Versuchen verwendeten Benzimidazole und deren N-Glykosiden wird beschrieben. F ür gewisse Milchsäurebakterien ist Vitamin B12

scholarly journals THE MICROBIOLOGICAL ASSAY OF VITAMIN B12 WITH LACTOBACILLUS LEICHMANNII

1949 ◽  
Vol 181 (2) ◽  
pp. 635-644
Author(s):  
C.E. Hoffmann ◽  
E.L.R. Stokstad ◽  
B.L. Hutchings ◽  
A.C. Dornbush ◽  
Thomas H. Jukes
Keyword(s):  
Vitamin B12 ◽  
Microbiological Assay ◽  
Lactobacillus Leichmannii

A Microbiological Assay for Vitamin B12 Using Lactobacillus leichmannii.

1949 ◽  
Vol 72 (2) ◽  
pp. 515-521 ◽  
Author(s):  
H. T. Peeler ◽  
H. Yacowitz ◽  
L. C. Norris
Keyword(s):  
Vitamin B12 ◽  
Microbiological Assay ◽  
Lactobacillus Leichmannii

scholarly journals Study on the microbiological assay for vitamin B12 of UHT cow's milk and infant formula using Lactobacillus leichmannii.

1989 ◽  
Vol 36 (5) ◽  
pp. 409-416 ◽  
Author(s):  
Toshiro KAMEI ◽  
Jun SATO ◽  
Yasuko SUGIMOTO ◽  
Naoko SUZUKI ◽  
Katsuhiko NODA
Keyword(s):  
Vitamin B12 ◽  
Infant Formula ◽  
Microbiological Assay ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Lactobacillus Leichmannii
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