Identification of putative migratory pheromones from Pacific lamprey (Lampetra tridentata)

2011 ◽  
Vol 68 (12) ◽  
pp. 2194-2203 ◽  
Author(s):  
Sang-Seon Yun ◽  
Andrew J. Wildbill ◽  
Michael J. Siefkes ◽  
Mary L. Moser ◽  
Andrew H. Dittman ◽  
...  
Keyword(s):  
Bile Acid ◽  
Columbia River Basin ◽  
State Agencies ◽  
Liquid Chromatography Mass Spectrometry ◽  
Pacific Lamprey ◽  
Petromyzonamine Disulfate ◽  
Petromyzosterol Disulfate ◽  
Petromyzonol Sulfate ◽  
The Pacific ◽  
Lampetra Tridentata

Pacific lamprey, Lampetra tridentata , has declined precipitously throughout their range in the Columbia River basin of North America. Tribal Nations and Federal and State agencies are engaged in efforts to restore these fish. Understanding whether Pacific lamprey emit and detect migratory pheromones is particularly important for these restoration efforts. Using behavioural assays, we demonstrated that migratory adult Pacific lamprey are attracted to odors emanating from their larval conspecifics. We then identified putative pheromones released by larval Pacific lamprey. Chemical analysis of the conditioned water from larval lamprey using liquid chromatography – mass spectrometry (LC–MS) revealed that the Pacific lamprey can release petromyzonamine disulfate (PADS), petromyzosterol disulfate (PSDS), and petromyzonol sulfate (PZS). Electro-olfactogram studies further demonstrated that adult Pacific lamprey can smell those bile acid compounds. Our data strongly indicate that the Pacific lamprey employ a chemical communication system mediated by a mixture of bile acids, as evidenced by pheromonal functions of the bile acid compounds in guiding migratory adult sea lamprey to the spawning streams. Comprehensive understanding of the chemical communications involved in lamprey migratory behavior may lead to improved scientific approaches for restoration efforts.

Nutritional Condition of the Pacific Lamprey (Lampetra tridentata) Deprived of Food for Periods of Up to Two Years

1993 ◽  
Vol 50 (3) ◽  
pp. 591-599 ◽  
Author(s):  
John N. C. Whyte ◽  
Richard J. Beamish ◽  
Norma G. Ginther ◽  
Chrys-Ellen Neville
Keyword(s):  
Nutritional Condition ◽  
Maintenance Energy ◽  
Fatty Acid Profiles ◽  
Lipid Levels ◽  
Pacific Lamprey ◽  
Spawning Areas ◽  
The Pacific ◽  
Endogenous Reserves ◽  
Adult Lamprey ◽  
Lampetra Tridentata

The anadromous parasitic Pacific lamprey (Lampetra tridentata) does not feed during metamorphosis or its spawning migration. To assess the utilization of body reserves, we compared the compositions of an adult lamprey held for 2 yr without food, recently metamorphosed lampreys, and lampreys starved for 6 mo. Moisture was higher and soluble ash and lipid levels were lower in tissue of the 2-yr-starved than in metamorphosed lampreys (2.67 and 3.39 kj∙g−1, respectively). Fatty acid profiles of 2-yr-starved and metamorphosed lampreys were qualitatively similar except for the presence of 15:0 in the latter. Substantially lower levels of 14:0 and 16:1 n7 and higher levels of polyunsaturated fatty acids occurred in starved lampreys. Changes in composition of lampreys starved for 6 mo were similar to changes between the 2-yr-starved and metamorphosed lampreys. Maintenance energy in a normalized 1-g lamprey starved for 6 mo was derived from catabolism of 71% lipid and 29% protein; total loss was 2.56 kJ or 49% of the original energy. The ability to survive extended periods on endogenous reserves, which may have allowed L. tridentata to survive past environmental catastrophes, allows it to migrate considerable distances to spawning areas in the headwaters of rivers.

Responses to rapid temperature change in the Pacific lamprey (Lampetra tridentata)

1985 ◽  
Vol 63 (5) ◽  
pp. 1027-1032
Author(s):  
Daniel E. Lemons ◽  
Larry I. Crawshaw
Keyword(s):  
Metabolic Effects ◽  
Mean Body Temperature ◽  
Thermal Biology ◽  
Pacific Lamprey ◽  
The Pacific ◽  
Rapid Temperature ◽  
Flow Through ◽  
Calorimetric Studies ◽  
Peripheral Temperature ◽  
Lampetra Tridentata

Some aspects of the thermal biology of the Pacific lamprey (Lampetra tridentata) were studied by making rapid changes in the ambient water temperature (Ta). Calorimetric studies were conducted to determine the rate of ensuing changes in mean body temperature [Formula: see text] and various deep body temperatures. Cardiovascular–respiratory alterations were evaluated in an experimental flow-through apparatus in which Ta could be rapidly shifted while changes in ventilation frequency (fV) and heart rate (fH) were recorded. Immediately following a step increase in Ta, the change in [Formula: see text] of a 0.21-kg lamprey was 33% complete in 33 s and 67% complete in 127 s. Deep dorsal muscle temperature changed much more slowly, being 33% complete in 87 s and 67% complete in 225 s. During rapid shifts in Ta, fV and fH changed at a significantly greater rate than would be predicted taking into account the change in [Formula: see text] and the resultant Q10 effects on metabolism. This effect persisted when the [Formula: see text] of the water was held at 600 Torr (1 Torr = 133.322 Pa). A possible interpretation of the results is that lampreys, like other water-breathing vertebrates, sense alterations in peripheral temperature and utilize this information to alter the output of the ventilatory and cardiovascular systems in a way that anticipates some of the metabolic effects of temperature.

Lentic Spawning by the Pacific Lamprey, Lampetra tridentata

1987 ◽  
Vol 44 (2) ◽  
pp. 476-478 ◽  
Author(s):  
J. E. Russell ◽  
F. W. H. Beamish ◽  
R. J. Beamish
Keyword(s):  
British Columbia ◽  
Wave Action ◽  
Pacific Lamprey ◽  
Lake System ◽  
Unidirectional Flow ◽  
The Pacific ◽  
Lotic Environments ◽  
Lampetra Tridentata ◽  
Lentic Water

Anadromous Pacific lamprey, Lampetra tridentata, typically construct nests and reproduce in lotic environments. In the summer of 1984, Pacific lamprey were observed spawning in shallow lentic water in two regions of the Babine Lake system, British Columbia. Nests were subject to wave action but an obvious unidirectional flow was not observed.

Low Mortality of Larval Lampreys from Electrofishing, Suction-pumping, Anesthesia, and Handling

2017 ◽  
Vol 8 (2) ◽  
pp. 640-647 ◽  
Author(s):  
Jeffrey C. Jolley ◽  
Christina T. Uh ◽  
Gregory S. Silver ◽  
Timothy A. Whitesel
Keyword(s):  
Pacific Northwest ◽  
Control Group ◽  
Long Term Effects ◽  
Negative Effects ◽  
Short Term ◽  
Important Species ◽  
Pacific Lamprey ◽  
The Pacific ◽  
Delayed Mortality

Abstract Native lamprey populations are declining worldwide. In the Pacific Northwest focus on conservation and management of these ecologically and culturally important species has increased. Concern has emerged regarding the effects of sampling and handling of lamprey, with little to no attention given to the larval lifestage. We monitored the survival of larval Pacific Lamprey Entosphenus tridentatus and Lampetra spp. after backpack electrofishing, deepwater electrofishing and suction-pumping, anesthesia, and handling. We performed survival trials on wild-caught lamprey (n = 15 larvae in each trial) collected from the Clackamas River drainage in Oregon, USA, coupled with control group trials from lamprey sourced from a hatchery (n = 10 larvae). Short-term (96 h) survival was >98% with only one observed mortality. Delayed mortality (1 wk) was observed for four individuals that had fungus; two of those were positive for the bacteria Aeromonas hyrdrophila. We recorded blood hematocrit as a secondary measure of stress. The baseline, nonstressed larvae hematocrit levels did not differ from those of fish that had undergone stress through electrofishing, suction-pumping, and handling without anesthesia. Electrofishing, suction-pumping, and anesthesia showed no short-term negative effects on larval lamprey although potential long-term effects remain unstudied. These techniques appear to provide efficient and relatively safe methods for collecting and surveying larval lamprey.

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