Contribution of commonly analyzed polycyclic aromatic hydrocarbons (PAHs) to potential toxicity in early life stages of rainbow trout (Oncorhynchus mykiss)

2006 ◽  
Vol 63 (6) ◽  
pp. 1320-1333 ◽  
Author(s):  
Henrik Sundberg ◽  
Rasha Ishaq ◽  
Ulla Tjärnlund ◽  
Gun Åkerman ◽  
Kerstin Grunder ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Aromatic Hydrocarbons ◽  
Environmental Protection Agency ◽  
Potential Toxicity ◽  
Total Extract ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Erod Induction ◽  
Polycyclic Aromatic

In a series of bio-effect-directed fractionation experiments, we investigated the potential toxicity of sediment extracts from a contaminated bay. A previous study investigated abnormalities and hepatic ethoxyresorufin O-deethylase (EROD) activities in rainbow trout (Oncorhynchus mykiss) larvae by exposing newly fertilized eggs to the total extract and to fractions separated by degree of aromaticity. A major part of the potential toxicity was isolated in a fraction containing polycyclic aromatic compounds (PACs). In this study, we prepared a synthetic PAC mixture with 17 commonly analyzed polycyclic aromatic hydrocarbons (PAHs) in amounts equimolar to those found in the sediment PAC fraction. The 17 PAHs, which included 11 of the 16 United States Environmental Protection Agency (US EPA) priority PAHs, were unable to account for the toxicopathic effects observed and could explain less than 4% of the total EROD induction. The lack of a clear relationship between toxicopathic effects and EROD induction underlines the need for a battery of biomarkers for estimating environmental risk. These results reveal the limits of our knowledge regarding compounds responsible for potential toxicity in field situations.

Use of the Rainbow Trout Hepatoma Cell Line, RTH-149, in a Cytotoxicity Assay

1989 ◽  
Vol 17 (2) ◽  
pp. 67-71
Author(s):  
Harvey Babich ◽  
Nieves Martin-Alguacil ◽  
Ellen Borenfreund
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Rainbow Trout ◽  
Cell Line ◽  
Aromatic Hydrocarbons ◽  
Hepatoma Cell ◽  
Neutral Red ◽  
Cytotoxicity Assay ◽  
Hepatoma Cell Line ◽  
Polycyclic Aromatic ◽  
Direct Acting

The rainbow trout hepatoma cell line, RTH-149, was evaluated for use as a bioindicator cell type in the neutral red cytotoxicity assay. The cells were exposed for six days to various polycyclic aromatic hydrocarbons, including chemicals that are direct-acting toxicants and chemicals that require enzymatic biotransformation to cytotoxic metabolites. Whereas benzo[a]pyrene was only slightly cytotoxic, its metabolites — (±)trans-7,8-diol-benzo[a]pyrene and 3-hydroxy-benzo[a]pyrene — were highly cytotoxic. 7,12-Dimethylbenz[a]anthracene was cytotoxic, but cytotoxicity did not occur with benzo[a]anthracene, benzo[b]fluoranthene and benzo[k]fluoranthene. This cell line appears to lack sufficient xenobiotic metabolising capacity to biotransform many of these polycyclic aromatic hydrocarbons to activated cytotoxic metabolites.

Estrogenic activity of multicyclic aromatic hydrocarbons in rainbow trout (Oncorhynchus mykiss) in vitro assays

2019 ◽  
Vol 207 ◽  
pp. 43-51 ◽  
Author(s):  
Richard C. Kolanczyk ◽  
Jeffrey S. Denny ◽  
Barbara R. Sheedy ◽  
Patricia K. Schmieder ◽  
Mark A. Tapper
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Aromatic Hydrocarbons ◽  
Estrogenic Activity ◽  
In Vitro Assays ◽  
Rainbow Trout Oncorhynchus Mykiss

Measurement, Source-Profiling and Potential Toxicity of Polycyclic Aromatic Hydrocarbons in an Agrarian Soil

2020 ◽  
Vol 7 (3) ◽  
pp. 827-844
Author(s):  
Adebanjo J. Anifowose ◽  
Ayomide O. Ogundola ◽  
Bolanle M. Babalola ◽  
Shola H. Awojide
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Potential Toxicity ◽  
Polycyclic Aromatic

Pollution analysis of soil polycyclic aromatic hydrocarbons from informal electronic waste dismantling areas in Xinqiao, China

2019 ◽  
Vol 37 (4) ◽  
pp. 394-401 ◽  
Author(s):  
Weihua Gu ◽  
Jianfeng Bai ◽  
Wenyi Yuan ◽  
En Ma ◽  
Chenglong Zhang ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Environmental Protection Agency ◽  
Electronic Waste ◽  
Sampling Location ◽  
Residential Areas ◽  
Potential Health ◽  
Sampling Locations ◽  
Polycyclic Aromatic ◽  
Standard Value

Polycyclic aromatic hydrocarbons (PAHs) are considered to be persistent organic pollutants, which pose a great threat to human health and the surrounding environment. In order to explore the influence of informal electronic waste (e-waste) dismantling activities on inhabitants who live nearby, soil samples were collected from informal e-waste dismantling areas in Xinqiao, China and analysed for 16 United States Environmental Protection Agency (USEPA) priority PAHs. Results indicated that the 16 USEPA priority PAHs were found at all seven sampling locations. Sampling location 3, which was only 10 m away from a residential area, had 1053.69 μg kg−1 of PAHs and seriously exceeded the standard value specified by the Netherlands. The total percents of 4-ring and 5-ring PAHs accounted for 61.74 and 71.70%, respectively, indicating that most of the detected PAHs belonged to high-ring PAHs. The informal e-waste dismantling activities are the major sources of soil PAHs in Xinqiao. Furthermore, the concentration of seven carcinogenic PAHs was 114.76 μg kg−1 and represented a potential health risk to humans. Thereinto, benzo[a]pyrene contributed the most, accounting for more than 50% in these locations. Our results may provide a reference about the influence of informal e-waste dismantling activities on the surrounding inhabitants and suggest that e-waste dismantling activities must be conducted in a formal enterprise which is far away from residential areas.

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