Planktonic Foraminifera — new dimensions with the Scanning Electron Microscope

1968 ◽  
Vol 5 (2) ◽  
pp. 231-233 ◽  
Author(s):  
Grant A. Bartlett

Extremely fine, detailed morphological characteristics of micro-organisms, hitherto unavailable, can now be determined with the Scanning Electron Microscope. Morphological characteristics related to evolutionary sequences, specific, generic, and familial relationships will permit more natural faunal classifications and enable more accurate ecological and biostratigraphic correlations.

2021 ◽  
Vol 9 (6) ◽  
pp. 1170
Author(s):  
Gabriel Haddad ◽  
Sara Bellali ◽  
Tatsuki Takakura ◽  
Anthony Fontanini ◽  
Yusuke Ominami ◽  
...  

Blood culture is currently the most commonly used method for diagnosing sepsis and bloodstream infections. However, the long turn-around-time to achieve microbe identification remains a major concern for clinical microbiology laboratories. Gram staining for preliminary identification remains the gold standard. We developed a new rapid strategy using a tabletop scanning electron microscope (SEM) and compared its performance with Gram staining for the detection of micro-organisms and preliminary identification directly from blood cultures. We first optimised the sample preparation for twelve samples simultaneously, saving time on imaging. In this work, SEM proved its ability to identify bacteria and yeasts in morphotypes up to the genus level in some cases. We blindly tested 1075 blood cultures and compared our results to the Gram staining preliminary identification, with MALDI-TOF/MS as a reference. This method presents major advantages such as a fast microbe identification, within an hour of the blood culture being detected positive, low preparation costs, and data traceability. This SEM identification strategy can be developed into an automated assay from the sample preparation, micrograph acquisition, and identification process. This strategy could revolutionise urgent microbiological diagnosis of infectious diseases.


Diversity ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 213
Author(s):  
M. Hossain ◽  
Pat Hutchings

A new species of glycerid polychaete, Glycera sheikhmujibi, is described from the saltmarsh on the central coast of Bangladesh. The species is identified based on morphological characteristics using both a light microscope and scanning electron microscope (SEM). The species is characterized by the presence of three distinct types of proboscideal papillae: type 1 papillae (conical with three transverse ridges), type 2 (conical with a straight, median, longitudinal ridge), and type 3 (round, shorter, and broader, with a straight, median, longitudinal ridge). It has a Y-shaped aileron with gently incised triangular base, almost equal-size digitiform noto- and neuropodial lobes in the mid-body, and long ventral cirri at the posterior end. The new species is compared with its related species, previously described from the Bay of Bengal region. A key to all these species is provided.


2020 ◽  
Vol 49 (1) ◽  
pp. 39-46
Author(s):  
Huan Xiong ◽  
De-Yi Yuan ◽  
Ya Chen ◽  
Genhua Niu ◽  
Feng Zou

The morphological characteristics of pollen grains of 16 cultivars belonging to two species of the genus Castanea (Castanea mollissima and C. henryi) collected from southern China were observed by using scanning electron microscope (SEM). The length of polar axis (PL), the equatorial diameter (ED), the length of colpus (CL) of grain, P/E ratio (polar axis to equatorial diameter), P/C ratio (polar axis to colpus length), and C/E ratio (colpus length to equatorial diameter) were measured and their variations were compared among studied taxa. The result of this study indicated that chestnut pollen grain was a tricolporate in aperture type, and aperprolate type in equatorial view. The morphological characteristics were mainly represented by PL, ED and CL as revealed by principal components analysis, which accounted for 99.56% of the total variations. The cluster analysis by the UPGMA tree divided the taxa into three groups and showed a great morphological affinity among the 16 cultivars.


Phytotaxa ◽  
2015 ◽  
Vol 237 (1) ◽  
pp. 1 ◽  
Author(s):  
Xin-Mao Zhou ◽  
Li-Jü Jiang ◽  
Liang Zhang ◽  
Xin-Fen Gao ◽  
Zhao-Rong He ◽  
...  

Using light microscope (LM) and scanning electron microscope (SEM), the megaspores and microspores of 77 samples representing ca. 70 species of Selaginella from China are observed. Combing previous studies, the spore morphology of nearly all documented Selaginella species from China were reviewed. Based on the morphological characteristics in megaspores and/or microspores, we divided the spores of Chinese species into 15 types and three types are further divided into various subtypes. Each type and subtype are described in detail and a key to the types and subtypes of spores is given. For the first time, the systematic significance of microspores of Selaginella are discussed, and the results indicate that microspores of Selaginella are significant in the systematics of Selaginella. Some important morphological characteristics in spores (e.g., color, micro-sculpture, size, etc.), often been neglected in previous studies, are introduced. Some spore-morphological synapomorphies of the clades and subclades, identified by recent molecular work (Zhou et al. 2015a), are well established. Using the spore morphology, the delimitation of some taxonomically difficult species in Selaginella is assessed.


Zootaxa ◽  
2004 ◽  
Vol 397 (1) ◽  
pp. 1 ◽  
Author(s):  
JUNGSUN YOO ◽  
JOOPIL KIM ◽  
HOZUMI TANAKA

Alopecosa volubilis n. sp. is described from Korea and is differentiated from other species in Alopecosa Simon, 1885 by morphological characteristics, such as three retromarginal teeth of chelicera, smaller body size, and the presence of a distinct tip of the median apophysis. The pedipalpal sclerites and somatic characters of two similar species, A. moriutii Tanaka, 1985 and A. hokkaidensis Tanaka, 1985, are compared with those of the new species. An illustration of the body and scanning electron microscope (SEM) pictures of the pedipalpal organ are provided.


2020 ◽  
Vol 39 (2) ◽  
pp. 93-115
Author(s):  
Marjorie Apthorpe

Abstract. The aim of this paper is to document three well-preserved morphotypes of Middle Jurassic (Bajocian) planktonic foraminifera from the continental margin of northwestern Australia. This location is on the southern shelf of the Middle Jurassic Tethys Ocean, and these occurrences of planktonic or meroplanktonic species are the first to be reported from the Jurassic of the Southern Hemisphere. The morphotypes include a new subspecies of Globuligerina bathoniana (Pazdrowa): Globuligerina bathoniana australiana n. ssp. Two other taxa are also described: Globuligerina altissapertura n. sp. and Mermaidogerina loopae n. gen. n. sp. The microstructure of the wall is shown in scanning electron microscope images. The change from chamber to chamber in the formation of the surface ornament by secondary lamination, and its subsequent burial within the wall, is demonstrated in detail.


1981 ◽  
Vol 64 (1) ◽  
pp. 199-224
Author(s):  
John E Kvenberg

Abstract Larval stored product beetle mandibles were studied by comparing images made by scanning electron microscopy with those made by conventional light microscopy. Discussion of morphological characteristics is based on illustrations of 25 species


2004 ◽  
Vol 39 (3) ◽  
pp. 297-300 ◽  
Author(s):  
Maria Angélica Pereira de Carvalho Costa ◽  
Weliton Antonio Bastos de Almeida ◽  
Francisco de Assis Alves Mourão Filho ◽  
Beatriz Madalena Januzzi Mendes ◽  
Adriana Pinheiro Martinelli Rodriguez

The objective of this work was to evaluate leaf epidermis morphological characteristics of three citrus somatic hybrids, compared to their parents. Parental and somatic hybrid young leaves were collected and processed for scanning electron microscope observations. Citrus polyploid hybrids have fewer stomata per area and these are larger compared to their diploid parental parents. No differences in internal arrangement of the stomatal cells were detected between parental plants and somatic hybrids. Additional studies may determine if these differences will influence physiological behavior of the plants in the field.


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