THE DETERMINATION OF ERGOTHIONEINE IN SIMPLE SOLUTION AND IN BLOOD

1949 ◽  
Vol 27e (4) ◽  
pp. 230-239 ◽  
Author(s):  
G. Hunter
Keyword(s):  
Acetic Acid ◽  
Human Blood ◽  
Lead Acetate ◽  
Reduced Glutathione ◽  
Simple Solution ◽  
Acetate Solution ◽  
Excess Lead ◽  
Photoelectric Colorimeter ◽  
Lead Acetate Solution

The diazo method of Hunter for the determination of ergothioneine in simple solution has been adapted to the Evelyn photoelectric colorimeter. The application of the method to the determination of ergothioneine in blood is described. Suitable blood filtrates are prepared by deproteinization with standard acetic acid oxalate solutions followed by removal of reduced glutathione with Goulard's lead acetate solution, and removal of excess lead with phosphate. By the method precise recovery is obtained of ergothioneine added to human blood.

Analytical Methods for Diethylstilbestrol in Feeds and Premixes

1966 ◽  
Vol 49 (5) ◽  
pp. 895-898
Author(s):  
Loyal R Stone
Keyword(s):  
Acetic Acid ◽  
Analytical Methods ◽  
Sodium Acetate ◽  
Buffer System ◽  
Official Method ◽  
Sodium Acetate Solution ◽  
Acetate Solution ◽  
Acid Buffer System ◽  
Acetic Acid Buffer

Abstract Methods are presented in which diethylstilbestrol is extracted from feeds in the Goldfisch apparatus, transferred into alkaline sodium acetate solution to avoid emulsions, and measured colorimetrically in a sodium acetate-acetic acid buffer system. The procedure is rapid, and results agree closely with those obtained by the official method. Procedures are also presented for determination of diethylstilbestrol in molasses and fat mixtures.

Modification of the Carbon Disulfide Evolution Method for Dithiocarbamate Residues

1969 ◽  
Vol 52 (1) ◽  
pp. 162-167 ◽  
Author(s):  
George E Keppel
Keyword(s):  
Hydrogen Sulfide ◽  
Hydrochloric Acid ◽  
Carbon Disulfide ◽  
Lead Acetate ◽  
Stannous Chloride ◽  
Acetate Solution ◽  
Colorimetric Measurement ◽  
Fungicide Residues ◽  
Mineral Acids ◽  
Lead Acetate Solution

Abstract A study was made of the analytical method for dithiocarbamate fungicide residues based on decomposition by hot mineral acids to the amine and carbon disulfide and colorimetric measurement of the carbon disulfide. Increased recoveries are obtained by the following modifications: adding a reducing agent (stannous chloride) to the sample before treatment with hot acid; svibstituting diluted sodium hydroxide for lead acetate solution to remove hydrogen sulfide and other interferences; and using boiling diluted hydrochloric acid. With these modifications, recoveries of N,N-dimethyldithiocarbamates from crops ranged from 85.3 to 103.8% (average 94.7%). Ethylenebisdithiocarbamates, with the exception of zineb (range 89.1–96.8%, average 92.0%), gave appreciably lower recoveries, indicating further study is necessary.

Selection of a Simple and Sensitive Method for Detecting Zearalenone in Corn

1994 ◽  
Vol 77 (4) ◽  
pp. 939-941 ◽  
Author(s):  
Nora M Quiroga ◽  
Inés Sola ◽  
Edith Varsavsky
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Lead Acetate ◽  
Limit Of Detection ◽  
Acetate Solution ◽  
Average Recovery ◽  
Lead Acetate Solution ◽  
Layer Chromatography ◽  
Selection Of ◽  
Sensitive Method

Abstract A simple, rapid, and sensitive method for determining zearalenone in corn was selected. The toxin was extracted from 50 g test portions with 180 mL acetonitrile and 20 mL 4% KCl solution. A portion of the extract was defatted with isooctane. The acetonitrile extract was cleaned up with 20% lead acetate solution. The zearalenone was partitioned into toluene. The toluene solution was dried, and the residue was redissolved in benzene. The toxin was determined by thin-layer chromatography with silica gel plates and chloroform–acetone (9 +1) as the developing solvent. The overall average recovery of zearalenone from corn was 97%. The limit of detection was 50 μg/kg; this limit may be lowered by using fast violet B salt as spray reagent. The method was compared with 2 previous methods that determine zearalenone in biologically contaminated corn.

scholarly journals EFFECT OF LEAD ON STRUCTURAL CHANGES IN LIVER OF WISTAR RATS UNDER THE CONDITIONS OF ACUTE EXPERIM

2021 ◽  
Vol 11 (2) ◽  
pp. 17-19
Author(s):  
Oleg Zayko ◽  
Vadim Astashov ◽  
Anna Sindireva ◽  
Karina Basnakyan ◽  
Arina Lukyanchikova
Keyword(s):  
Lead Acetate ◽  
Wistar Rats ◽  
Structural Changes ◽  
Structural Transformations ◽  
Acute Experiment ◽  
Acetate Solution ◽  
Hydropic Degeneration ◽  
Histological Studies ◽  
Lead Salts ◽  
Lead Acetate Solution

The purpose of this study was to evaluate the structural transformations of Wistar rats liver after oral administration of lead salts. Under the conditions of acute experiment during 5 days the Wistar rats were orally administered the lead acetate solution in the amount of 3 mg/kg. Histological studies were carried out at OSMU upon the completion of the experiment. Effect of toxic doses of lead causes symptoms of both steatosis and hydropic degeneration of the liver. It is assumed that the appearance of hepatic steatosis reflects the reaction of hepatocytes to hemic hypoxia caused by the action of lead, while the signs of hydropic degeneration expressed in varying degrees reflect its direct toxic effect.

Rapid Procedure for Aflatoxin Analysis in Cottonseed Products

1966 ◽  
Vol 49 (4) ◽  
pp. 740-743
Author(s):  
Leonard Stoloff ◽  
Alan Graff ◽  
Harold Rich
Keyword(s):  
Acetic Acid ◽  
Cottonseed Meal ◽  
Lead Acetate ◽  
Aqueous Acetone ◽  
Water Mixture ◽  
Partition Chromatography ◽  
Good Recovery ◽  
Powdered Cellulose ◽  
Rapid Procedure

Abstract A new method for the determination of aflatoxins in cottonseed is presented and compared with two other procedures. This procedure yields good recovery for added aflatoxin, is relatively simple and rapid, and produces relatively clean extracts for TLC. A 75 g sample of ground cottonseed or cottonseed meal is extracted with aqueous acetone, centrifuged to remove solids, treated with lead acetate and acetic acid to precipitate interfering substances, and recentrifuged. An aliquot of the supernatant is mixed with powdered cellulose and packed into a chromatographic column for liquid-liquid partition chromatography. Hexane elutes the lipid materials; the aflatoxins are eluted with a hexane-chloroform mixture. After solvent evaporation, the aflatoxin-containing residue is dissolved in chloroform and chromatographed by TLC. Silica gel is the adsorbent; a benzeneethanol-water mixture is the developing solvent. The aflatoxin content is estimated by comparison with standard aflatoxins.

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