THE ISOLATION OF A BLUE FLUORESCENT COMPOUND SCOPOLETIN, FROM GREEN MOUNTAIN POTATO TUBERS, INFECTED WITH LEAF ROLL VIRUS

1948 ◽  
Vol 26c (1) ◽  
pp. 31-34 ◽  
Author(s):  
W. A. Andreae
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Leaf Roll ◽  
First Year ◽  
Potato Tubers ◽  
Green Mountain ◽  
Fluorescent Compound ◽  
Tobacco Plants ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

A method has been described for the isolation of a crystalline fluorescent compound that occurs in Green Mountain potato tubers during the first year of infection with leaf roll virus. The substance was identified as 7-hydroxy-6-methoxy 1:2 benzopyrone (scopoletin), previously isolated by Best from tobacco plants, infected with tomato spotted wilt virus.

scholarly journals Pathogen-derived Resistance to Tomato Spotted Wilt Virus in Transgenic Tomato and Tobacco Plants

2005 ◽  
Vol 130 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Olga Fedorowicz ◽  
Grzegorz Bartoszewski ◽  
Maria Kamińska ◽  
Pravda Stoeva ◽  
Katarzyna Niemirowicz-Szczytt
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Yield Losses ◽  
Sources Of Resistance ◽  
Tobacco Plants ◽  
Tomato Spotted Wilt ◽  
Significant Yield ◽  
Pathogen Derived Resistance ◽  
Wilt Virus ◽  
Primary Transformants ◽  
Translational Product

This study was undertaken to remedy significant yield losses in commercial tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana tabacum L.) production caused by tomato spotted wilt virus (TSWV). One of the possible sources of resistance can be incorporation into the host plant of a viral nucleoprotein (N) gene by Agrobacterium-mediated transformation. Twelve primary transformants of tomato and 141 of tobacco were analyzed for the expression of the N gene and for resistance to the TSWV infection. The tests have demonstrated that transgenic plants were protected against virus infection irrespective of whether or not they contained detectable levels of the translational product.

scholarly journals VIRAL PROTECTION IN TRANSGENIC TOBACCO PLANTS EXPRESSING THE TOMATO SPOTTED WILT VIRUS N GENE

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 621b-621
Author(s):  
Jong Wook Kim ◽  
Thomas L. German ◽  
Samuel S. M. Sun
Keyword(s):  
Transgenic Tobacco ◽  
Blot Analysis ◽  
Tomato Spotted Wilt Virus ◽  
First Generation ◽  
N Gene ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
N Protein ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Nucleocapsid protein (N) gene was isolated from tomato spotted wilt virus (TSWV) Hawaiian L isolate, and introduced into Nicotiana tabacum cv. Xanthi nc in order to test for “CP-mediated protection”. Agrobacterium tumefaciens-mediated transformation was performed. The integrity and the expression of N gene were verified by Southern blot and Northern blot analysis, and the N protein in the transgenic tobacco plants were determined by ELISA and Western blot analysis. Several first generation of transgenic tobacco were tested for virus resistance. Comparably smaller numbers of the local lesions were developed with several day of delay in the in-frame transformants.

Characterization of RNA–Mediated Resistance to Tomato Spotted Wilt Virus in Transgenic Tobacco Plants

1992 ◽  
Vol 10 (10) ◽  
pp. 1133-1137 ◽  
Author(s):  
Peter de Haan ◽  
Jan J. L. Gielen ◽  
Marcel Prins ◽  
Ineke G. Wijkamp ◽  
Alie van Schepen ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Tomato Spotted Wilt Virus ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Tomato Spotted Wilt ◽  
Wilt Virus
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