ON STAINING FOR VITAMIN C IN TISSUES

1946 ◽  
Vol 24c (5) ◽  
pp. 182-187 ◽  
Author(s):  
Jules Tuba ◽  
George Hunter ◽  
John A. Osborne
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Guinea Pig ◽  
Acid Content ◽  
Ascorbic Acid Content ◽  
Zona Fasciculata ◽  
Staining Solution ◽  
Apparent Relationship ◽  
Gradual Accumulation ◽  
The Rose

Guinea-pig adrenals were stained for ascorbic acid. In the normal adrenal, staining was found in both cortex and medulla though with greatest density in the zona fasciculata. The stained scorbutic adrenal showed only a few granules.The mosses Mnium cuspidatum and Mnium affine contain ascorbic acid in appreciable amounts. Following staining, fine black granules could be seen in both species. The stain intensity of the granules roughly corresponded to their relative ascorbic acid content.Having satisfactorily established the validity of the staining solution by the above experiments the development of ascorbic acid in the rose hips of R. acicularis was followed throughout the summer. There was found to be some association between the ascorbic acid and chloroplasts. With increasing maturity there developed a gradual accumulation of fine granules in the cells of the tissue as well. These granules showed no apparent relationship to any formed elements in the cells. Staining densities roughly parallel ascorbic acid content.

Vitamin C activity of D-isoascorbic acid for the guinea pig

1969 ◽  
Vol 47 (12) ◽  
pp. 985-991 ◽  
Author(s):  
Omer Pelletier ◽  
Claude Godin
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Guinea Pig ◽  
Small Proportion ◽  
Acid Content ◽  
Guinea Pigs ◽  
Ascorbic Acid Content ◽  
Similar Response ◽  
Synthetic Diet ◽  
Isoascorbic Acid

Guinea pigs fed with a synthetic diet or a rabbit ration were depleted of vitamin C for 17 days. The scorbutic animals were treated daily during 2 months with 40 mg D-isoascorbic acid (IAA) or 2 mg L-ascorbic acid (AA). Both isomers restored the growth of the animals and caused the disappearance of scorbutic symptoms. For 3 weeks, the two diets gave similar response, after which the animals fed with the rabbit ration grew much less. Animals given IAA ate less and had smaller weight gains than those given AA; this effect was overcome by pair-feeding. Only a small proportion of administered IAA was recovered in the organs and in the urine. The total ascorbic acid content (AA and IAA) of the IAA-treated animals was less than that of the AA-treated animals. The low AA content of the organs of the IAA-treated animals indicated that IAA had no significant sparing action on AA.

COMPARISON OF ASCORBIC ACID CONTENT IN IMPORTED TOMATOES AND IN CULTIVARS GROWN IN NOVA SCOTIA

1987 ◽  
Vol 67 (1) ◽  
pp. 331-335
Author(s):  
HAK-YOON JU ◽  
W. JOHN MULLIN
Keyword(s):  
Ascorbic Acid ◽  
Nova Scotia ◽  
Vitamin C ◽  
Acid Content ◽  
Ascorbic Acid Content ◽  
Fresh Weight ◽  
Atlantic Region ◽  
Significant Difference ◽  
Cluster Location ◽  
Greenhouse Tomatoes

The ascorbic acid (vitamin C) content of fresh imported field tomatoes and Nova Scotia greenhouse and field tomatoes was determined on a bi-weekly basis during the period of availability of each type of tomato to the Nova Scotia consumer in 1984. The average ascorbic acid contents of imported and Nova Scotia field and greenhouse tomatoes were 13.3, 16.7 and 17.7 mg 100 g−1 fresh weight, respectively. A study of nine recommended or promising field tomatoes for the Atlantic region showed significant differences in ascorbic acid content among the cultivars. The cultivar Quick Pick had the highest ascorbic acid content of 22.5 ± 1.5 mg 100 g−1, the cultivar Campbell 18 had the lowest content, 12.0 ± 2.9 mg 100 g−1. In Dombito greenhouse tomatoes the stage of maturity and the effect of cluster location were tested against ascorbic acid content. The lowest ascorbic acid content of 9.1 ± 1.0 mg 100 g−1 was found with the small green tomatoes while others from mature green to overripe contained 14.0–16.7 mg 100 g−1. Tomatoes from different cluster locations showed no significant difference in ascorbic acid content.Key words: Vitamin C, L-ascorbic acid, tomatoes

The Effects of Breed Characteristics and Stages of Lactation on the Vitamin C (Ascorbic Acid) Content of Cow's Milk

1936 ◽  
Vol 11 (5) ◽  
pp. 425-432 ◽  
Author(s):  
Russel Rasmussen ◽  
N. B. Guerrant ◽  
A. O. Shaw ◽  
R. C. Welch ◽  
S. I. Bechdel
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Acid Content ◽  
Ascorbic Acid Content ◽  
Cow’S Milk ◽  
Cow's Milk

scholarly journals Ascorbic acid in the fruits and leaves of some persimmon cultivars in the Southern Coast of the Crimea

2021 ◽  
pp. 116-120
Author(s):  
O. A. Grebennikova ◽  
V. A. Melnikov
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Acid Content ◽  
Ascorbic Acid Content ◽  
Southern Coast ◽  
Additional Source ◽  
Botanical Gardens ◽  
The Crimea ◽  
Maximum Content

The results of the ascorbic acid content in the fruits and leaves of 10 persimmon cultivars from the Nikitsky Botanical Gardens’ collection are presented. It has been established that the concentration of ascorbic acid in technical persimmon fruits is 19,8-56,3 mg / 100 g, and in mature fruits it is 70-85% lower (5,08-8,96 mg / 100 g). Persimmon leaves contain 5-20 times more ascorbic acid than in fruits. The results showed the ability to use persimmon leaves as an additional source of vitamin C. The maximum content of ascorbic acid is allocated to the fruits of the cultivars Virginskaya Krupnoplodnaya, Delishes, Sidles and leaves of the cultivars Sidles and Zolotistaya.

Total phenol, total flavonoid and ascorbic acid content of Iranian commercial orange juice

2021 ◽  
Author(s):  
Hoda Ashari ◽  
Naficeh Sadeghi ◽  
Mohammad Reza Oveisi ◽  
Mannan Hajimahmoodi
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Acid Content ◽  
Orange Juice ◽  
Ascorbic Acid Content ◽  
Total Phenol ◽  
Spectrometric Method ◽  
Total Phenols ◽  
Significant Difference ◽  
Gallic Acid Equivalent

This study was designed to measure and compare the total phenols, flavonoids and vitamin C contents in four orange juice brands which are commercially available in Iran. Total phenols, flavonoids and ascorbic acid content of 100 samples from four different commercial brands were evaluated by the spectrometric method. The concentration of total phenol in commercial orange juice samples was between 28.39 and 114.20 mg gallic acid equivalent per liter (mg GAE/L). The measured range of total flavonoids was from 12.53 to 32.62 mg quercetin equivalent per liter (mg QE/L) and the content of ascorbic acid in the samples was between 29.95 and 93.08 mg/L. The results showed a significant difference between the four brand`s total phenols, flavonoids and vitamin C level (P< 0.05). According to the found variation among different studied brands, setting a determined amount for the measured parameters is suggested.

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