THE EFFECT OF TEMPERATURE ON THE EXPRESSION OF FACTORS GOVERNING RUST REACTION IN A CROSS BETWEEN TWO VARIETIES OF TRITICUM VULGARE
Two random populations of F2 plants of the cross Marquillo × Marquis were tested for the reaction of their F3 seedling progenies to form 21 of Puccinia graminis tritici in the greenhouse, at average daily temperatures of 69.7° F. (the warm test) for one population, and 60.6° F. (the cool test) for the other. In both tests Marquis was susceptible and Marquillo was resistant. In the "warm test" ten families of a total of 781 were resistant. In the "cool test" five families of a total of 301 were susceptible. In both cases the results fitted a 63:1 ratio excellently, indicating the operation of three main genetic factors for rust reaction. A genetic hypothesis is proposed that explains the results on the basis of the influence of low temperature in curtailing the action of three susceptibility factors A, B and C carried by Marquis. The results indicate that genetic studies on characters which are easily influenced by environmental conditions should be made under controlled conditions, after ascertaining in advance the general effects of different temperatures, etc., upon the hybrid material to be used.