Molecular cloning and induced expression of the plasma membrane intrinsic protein gene and promoter from mulberry (Morus multicaulis)

2018 ◽  
Vol 98 (6) ◽  
pp. 1245-1253
Author(s):  
Wang Heng ◽  
Chen Dandan ◽  
Zhou Hong ◽  
Ackon Justice Afriyie ◽  
Dominic Kwame Kotoka ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Binding Sites ◽  
Molecular Mechanisms ◽  
Pcr Analysis ◽  
Flanking Sequence ◽  
Plasma Membrane Intrinsic Protein ◽  
Intrinsic Protein ◽  
Qrt Pcr ◽  
Strong Homology ◽  
Polymerase Chain

To isolate the plasma membrane intrinsic protein 2 (PIP2) gene and its promoter from mulberry (Morus multicaulis), and to analyse its expression under stress conditions using quantitative real-time polymerase chain reaction (qRT-PCR) analysis, the mulberry MmPIP2 gene was cloned and sequenced. The putative MmPIP2 protein was 282 amino acids long and contained Asn–Pro–Ala signature motifs. Phylogenetic analysis showed that MmPIP2 is highly conserved, exhibiting strong homology with other plant PIP2s. The 5′ flanking sequence was cloned by genome walking and numerous transcription factor binding sites were identified. qRT-PCR revealed that the expression levels of MmPIP2 differed with changes in abiotic stress, indicating that PIP2 is a multifunctional molecule in mulberry. Studying the molecular mechanisms behind adaptations to stress and strengthening stress tolerance in plants are of fundamental importance to mulberry production.

Ectopic expression of a rice plasma membrane intrinsic protein (OsPIP1;3) promotes plant growth and water uptake

2020 ◽  
Vol 102 (4) ◽  
pp. 779-796 ◽  
Author(s):  
Siyu Liu ◽  
Tatsuya Fukumoto ◽  
Patrizia Gena ◽  
Peng Feng ◽  
Qi Sun ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Plant Growth ◽  
Water Uptake ◽  
Ectopic Expression ◽  
Plasma Membrane Intrinsic Protein ◽  
Intrinsic Protein

scholarly journals Transcriptome analysis of two Pogostemon cablin chemotypes reveals genes related to patchouli alcohol biosynthesis

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12025
Author(s):  
Wuping Yan ◽  
Zhouchen Ye ◽  
Shijia Cao ◽  
Guanglong Yao ◽  
Jing Yu ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Differentially Expressed ◽  
Perennial Herb ◽  
Pcr Analysis ◽  
Biosynthetic Pathways ◽  
Rna Seq ◽  
Patchouli Alcohol ◽  
Pogostemon Cablin ◽  
Polymerase Chain ◽  
Distribution Frequency

Pogostemon cablin, a medicinally and economically important perennial herb, is cultivated around the world due to its medicinal and aromatic properties. Different P. cablin cultivars exhibit different morphological traits and patchouli oil components and contents (especially patchouli alcohol (PA) and pogostone (PO)). According to the signature constituent of the leaf, P. cablin was classified into two different chemotypes, including PA-type and PO-type. To better understand the molecular mechanisms of PA biosynthesis, the transcriptomes of Chinese-cultivated P. cablin cv. PA-type “Nanxiang” (NX) and PO-type “Paixiang” (PX) were analyzed and compared with ribonucleic acid sequencing (RNA-Seq) technology. We obtained a total of 36.83 G clean bases from the two chemotypes, compared them with seven databases and revealed 45,394 annotated unigenes. Thirty-six candidate unigenes participating in the biosynthesis of PA were found in the P. cablin transcriptomes. Overall, 8,390 differentially expressed unigenes were identified between the chemotypes, including 2,467 upregulated and 5,923 downregulated unigenes. Furthermore, six and nine differentially expressed genes (DEGs) were mapped to the terpenoid backbone biosynthetic and sesquiterpenoid and triterpenoid biosynthetic pathways, respectively. One key sesquiterpene synthase gene involved in the sesquiterpenoid and triterpenoid biosynthetic pathways, encoding patchoulol synthase variant 1, was significantly upregulated in NX. Additionally, GC-MS analysis of the two chemotypes in this study showed that the content of PA in NX was significantly higher than that of PX, while the content of PO showed the opposite phenotype. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the DEG expression tendency was consistent with the transcriptome sequencing results. Overall, 23 AP2/ERF, 13 bHLH, 11 MYB, 11 NAC, three Trihelix, 10 WRKY and three bZIP genes that were differentially expressed may act as regulators of terpenoid biosynthesis. Altogether, 8,314 SSRs were recognized within 6,825 unigenes, with a distribution frequency of 18.32%, among which 1,202 unigenes contained more than one SSR. The transcriptomic characteristics of the two P. cablin chemotypes are comprehensively reported in this study, and these results will contribute to a better understanding of the molecular mechanism of PA biosynthesis. Our transcriptome data also provide a valuable genetic resource for further studies on P. cablin.

scholarly journals Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family

Cell Research ◽  
2006 ◽  
Vol 16 (3) ◽  
pp. 277-286 ◽  
Author(s):  
Lei Guo ◽  
Zi Yi Wang ◽  
Hong Lin ◽  
Wei Er Cui ◽  
Jun Chen ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Plasma Membrane ◽  
Gene Family ◽  
Protein Gene ◽  
Plasma Membrane Intrinsic Protein ◽  
Intrinsic Protein
Sign in / Sign up

Export Citation Format

Share Document