Perinatal hypothyroidism modulates antioxidant defence status in the developing rat liver and heart

2017 ◽  
Vol 95 (2) ◽  
pp. 185-189 ◽  
Author(s):  
Hongmei Zhang ◽  
Yan Dong ◽  
Qing Su
Keyword(s):  
Oxidative Stress ◽  
Rat Liver ◽  
Antioxidant Defence ◽  
Sod Activity ◽  
Oxidative Stress Status ◽  
Total Antioxidant ◽  
Perinatal Hypothyroidism ◽  
Developing Rat ◽  
Increased Activity ◽  
Gpx Activity

In the present study, we investigated oxidative stress parameters and antioxidant defence status in perinatal hypothyroid rat liver and heart. We found that the proteincarbonyl content did not differ significantly between the three groups both in the pup liver and in the heart. The OH˙ level was significantly decreased in the hypothyroid heart but not in the liver compared with controls. A slight but not significant decrease in SOD activity was observed in both perinatal hypothyroid liver and heart. A significantly increased activity of CAT was observed in the liver but not in the heart of hypothyroid pups. The GPx activity was considerably increased compared with controls in the perinatal hypothyroid heart and was unaltered in the liver of hypothyroid pups. We also found that vitamin E levels in the liver decreased significantly in hypothyroidism and were unaltered in the heart of perinatal hypothyroid rats. The GSH content was elevated significantly in both hypothyroid liver and heart. The total antioxidant capacity was higher in the liver of the hypothyroid group but not in the hypothyroid heart. Thyroxine replacement could not repair the above changes to normal. In conclusion, perinatal hypothyroidism modulates the oxidative stress status of the perinatal liver and heart.

scholarly journals Buprenorphine and Methadone as Opioid Maintenance Treatments for Heroin-Addicted Patients Induce Oxidative Stress in Blood

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Christonikos Leventelis ◽  
Nikolaos Goutzourelas ◽  
Aikaterini Kortsinidou ◽  
Ypatios Spanidis ◽  
Georgia Toulia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Redox Status ◽  
Protein Carbonyls ◽  
Healthy Individuals ◽  
Thiobarbituric Acid Reactive Substances ◽  
Antioxidant Defence ◽  
Total Antioxidant ◽  
Beneficial Action ◽  
The Impact

Buprenorphine and methadone are two substances widely used in the substitution treatment of patients who are addicted to opioids. Although it is known that they partly act efficiently towards this direction, there is no evidence regarding their effects on the redox status of patients, a mechanism that could potentially improve their action. Therefore, the aim of the present investigation was to examine the impact of buprenorphine and methadone, which are administered as substitutes to heroin-dependent patients on specific redox biomarkers in the blood. From the results obtained, both the buprenorphine (n=21) and the methadone (n=21) groups exhibited oxidative stress and compromised antioxidant defence. This was evident by the decreased glutathione (GSH) concentration and catalase activity in erythrocytes and the increased concentrations of thiobarbituric acid reactive substances (TBARS) and protein carbonyls in the plasma, while there was no significant alteration of plasma total antioxidant capacity (TAC) compared to the healthy individuals (n=29). Furthermore, methadone revealed more severe oxidant action compared to buprenorphine. Based on relevant studies, the tested substitutes mitigate the detrimental effects of heroin on patient redox status; still it appears that they need to be boosted. Therefore, concomitant antioxidant administration could potentially enhance their beneficial action, and most probably, buprenorphine that did not induce oxidative stress in such a severe mode as methadone, on the regulation of blood redox status.

scholarly journals Poor Glycaemic Control Is Associated with Increased Lipid Peroxidation and Glutathione Peroxidase Activity in Type 2 Diabetes Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Harshi Prasadini Gunawardena ◽  
Renuka Silva ◽  
Ramiah Sivakanesan ◽  
Pathmasiri Ranasinghe ◽  
Prasad Katulanda
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Glycaemic Control ◽  
Study Groups ◽  
Cellular Damage ◽  
Lower Plasma ◽  
Sod Activity ◽  
Compensatory Response ◽  
Gpx Activity

Glycaemic control is the main focus of managing diabetes and its complications. Hyperglycaemia induces oxidative stress favouring cellular damage and subsequent diabetic complications. The present study was conducted to compare the plasma total antioxidant capacity (TAC) and individual antioxidant marker antioxidant status of type 2 diabetics (T2D) with good ((+) GC) and poor ((-) GC) glycaemic control with prediabetic (PDM) and normoglycaemic (NG) individuals. T2D (n=147), PDM (n=47), and NGC (n=106) were recruited as subjects. T2D and PDM had lower plasma TAG than NG subjects. T2D and PDM had significantly higher GPx activity and plasma MDA concentrations than NG. PDM showed the highest SOD activity. T2D (-) GC showed significantly elevated GPx activity and higher MDA level and significantly lower SOD activity among all study groups. Lower plasma TAC and higher plasma MDA indicate the presence of oxidative stress in T2D and PDM. Elevated GPx activity in T2D, PDM, and particularly in T2D (-) GC suggests a compensatory response to counteract excess lipid peroxidation in the hyperglycaemic state. Decline in SOD activity advocates the presence of glycation and excess lipid peroxidation in T2D.

scholarly journals Panel of Oxidative Stress and Inflammatory Biomarkers in ALS: A Pilot Study

2016 ◽  
Vol 44 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Hélène Blasco ◽  
Guillaume Garcon ◽  
Franck Patin ◽  
Charlotte Veyrat-Durebex ◽  
Judith Boyer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Clinical Status ◽  
Total Antioxidant Status ◽  
Routine Practice ◽  
Clinical Markers ◽  
Sod Activity ◽  
Total Antioxidant ◽  
Preliminary Study ◽  
Als Patients ◽  
Lateral Sclerosis

AbstractBackground: Pathophysiological mechanisms that contribute to neurodegeneration in Amyotrophic Lateral Sclerosis (ALS) include oxidative stress and inflammation. We conducted a preliminary study to explore these mechanisms, to discuss their link in ALS, and to determine the feasibility of incorporating this combined analysis into current biomarkers research. Methods: We enrolled 10 ALS patients and 10 controls. We measured the activities of glutathione peroxidase, glutathione reductase, superoxyde dismutase (SOD), and the levels of serum total antioxidant status (TAS), malondialdehyde (MDA), 8-hydroxy-2’-deoxyguanosine (8-OHdG), and glutathione status (e.g. glutathione disulfide, GSSG/reduced glutathione, GSH). We analysed the concentrations of homocysteine, several cytokines, vitamins and metals by standard methods used in routine practice. Results: There was a significant decrease in TAS levels (p=0.027) and increase in 8-OHdG (p=0.014) and MDA (p=0.011) levels in ALS patients. We also observed a significantly higher GSSG/GSH ratio (p=0.022), and IL-6 (p=0.0079) and IL-8 (p=0.009) concentrations in ALS patients. Correlations were found between biological and clinical markers (homosysteine vs. clinical status at diagnosis, p=0.02) and between some biological markers such as IL-6 vs. GSSG/GSH (p=0.045) or SOD activity (p=0.017). Conclusion: We confirmed the systemic alteration of both the redox and the inflammation status in ALS patients, and we observed a link with some clinical parameters. These promising results encourage us to pursue this study with collection of combined oxidative stress and inflammatory markers.

scholarly journals Effect of Treatment with Peptide Extract from Beef Myofibrillar Protein on Oxidative Stress in the Brains of Spontaneously Hypertensive Rats

Foods ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 455
Author(s):  
Lee ◽  
Hur
Keyword(s):  
Oxidative Stress ◽  
Spontaneously Hypertensive Rats ◽  
Myofibrillar Protein ◽  
Oxygen Species ◽  
Hypertensive Rats ◽  
Sod Activity ◽  
Spontaneously Hypertensive ◽  
Hypertension Therapy ◽  
Effect Of Treatment ◽  
Gpx Activity

This study was conducted to determine the effect of beef peptide extract on oxidative stress in the brains of spontaneously hypertensive rats (SHRs). A 3-kDa peptide extract was obtained from beef myofibrillar protein using alkaline-AK (AK3K). Oxidative stress in SHR brains was measured by assessing malondialdehyde (MDA) and reactive oxygen species (ROS) concentrations and superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activity. The SHR brains treated with the AK3K peptide extract (400 mg/kg body weight, AK3K400) showed a significant decrease in MDA and ROS contents by 0.33 and 23.92 μM, respectively (p < 0.05) compared to the control. The SOD activity for AK3K400 was 61.26%, around 20% higher than the control. Furthermore, the SHRs treated with the AK3K peptide extract showed results similar to those obtained using captopril, a hypertension drug, except for the MDA level. The study demonstrates that the beef peptide extract inhibits the generation of oxidative stress in the SHR brain and could possibly be used for neuronal hypertension therapy.

The state of the blood antioxidant system in the patients presenting with acromegaly

2015 ◽  
Vol 61 (2) ◽  
pp. 8-11
Author(s):  
M V Faassen ◽  
M S Pankratova ◽  
N N Molitvoslovova ◽  
A A Baizhumanov ◽  
S S Kovalenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Plasma ◽  
Antioxidant System ◽  
The State ◽  
Total Antioxidant Activity ◽  
Sod Activity ◽  
Protein Thiols ◽  
Total Antioxidant

The state of the blood antioxidant system in the patients presenting with acromegaly. The objective of the present study was to evaluate the total antioxidant activity (TAA) of blood plasma, the levels of non-protein thiols and 2-thyobarbituric acid-active products (TBA-AP), superoxide dismutase (SOD) and catalase (CAT) activities as well as ceruloplasmin (CP) level in the patients presenting with acromegaly. It was shown that plasma TAA and SOD activity in this patients was on the average 20 and 30% lower respectively than in the control subjects. At the same time, the TBA-AP and CP levels increased by 50 and 40% respectively. These data suggest the development of oxidative stress in the acromegalic patients.

scholarly journals The Antioxidant Effect of Beta-Alanine or Carnosine Supplementation on Exercise-Induced Oxidative Stress: A Systematic Review and Meta-Analysis

2018 ◽  
Author(s):  
Elias de França ◽  
Fábio Santos Lira ◽  
Marcio Flávio Ruaro ◽  
Vinicius Barroso Hirota ◽  
Paula A. Faria Waziry ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Systematic Review ◽  
Meta Analysis ◽  
Protein Carbonyl ◽  
Human Model ◽  
Sod Activity ◽  
Beta Alanine ◽  
Total Antioxidant ◽  
Acute Increase ◽  
Exercise Induced

The objective of this study was to perform a systematic review and meta-analysis of the articles that addressed the effect beta-alanine (BA) or carnosine supplementation on Physical exercise (PE)-induced oxidative stress (OS). We searched throughout PubMed, CAPES Periodic and SPORTDiscus human model peer review, randomized control studies with chronic BA or carnosine supplementation on PE-induced OS. We search papers published before May 2018.&nbsp; A total of 128 citations were found. Only four articles met criteria for inclusion. All four studies used healthy young (21y) sedentary, recreationally active or athletic participants. After a chorionic BA (~30 days) or carnosine (14 days) supplementation, the studies evaluated PE-induced OS both immediately and several hours after exercise (0.5 to 48 h). In response to PE-induced OS, BA/carnosine supplementation increased total antioxidant capacity (TAC) and glutathione concentrations while decreased pro-oxidant markers and superoxide dismutase (SOD) activity. BA or carnosine supplementation did not prevent the increase in peroxidation markers (e.g., 8-isoprostane, protein carbonyl or malonaldehyde). In humans, following PE-induced OS, initial treatment trials of BA or carnosine supplementation seemed to increase TAC and GSH concentrations, while decreasing SOD activity. Also, albeit mitigating the acute increase in pro-oxidants, treatment did not decrease measured values of peroxidation markers.

scholarly journals The Effect of Exercise Intensity and Duration on Oxidative Stress and Antioxidant Enzymes Activity among Sedentary Healthy Adults: A Repeated Measures Study

2021 ◽  
Author(s):  
D.Maryama Ag.Daud ◽  
Fatimah Ahmedy ◽  
Dg. Marshitah Pg. Baharuddin ◽  
Tengku Fadilah Tengku Kamalden ◽  
Wan Zurinah Wan Ngah
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Exercise Intensity ◽  
Repeated Measures ◽  
Exercise Duration ◽  
Constant Load ◽  
Physiological Adaptation ◽  
Sod Activity ◽  
Oxidative Stress Status ◽  
Major Controlling Factor

Abstract Background Aerobic exercise can increase oxidative stress, but it can produce the necessary stimulus for physiological adaptation of exercise. However, the effects of intensity and duration of exercise on oxidative stress status are unclear. This study aimed to compare the effects of exercise intensity (I) and duration (D) on the oxidative stress [malondialdehyde (MDA)], and the responses of the antioxidant enzymes [catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), antioxidant enzymes ratio (AE)] among sedentary adults. Methods In a randomized crossover design, 25 sedentary adults, performed nine cycling exercise sessions with a constant load of 50%, 60% and 70% VO2peak for 10-, 20- and 30- minutes duration. Plasma MDA, CAT, GPx and SOD activity were measured before exercise (baseline) and immediately after each session (post). Results The interaction effect of intensity and duration was significant for percentage changes of MDA (FIxD=3.59, df=4, p<0.05) and CAT activity (FIxD =3.38, df=2.146, p<0.05). Repeated Measures ANOVA analysis revealed that intensity is the major controlling factor for MDA (FI =54.24, df=2, p<0.05 vs FD=8.62, df=2, p<0.05), and CAT responses (FI =14.24, df=1.619, p<0.05 vs FD = 5.96, df=1.347, p<0.05). However, the main determinant factor for SOD (FD =11.82, df=1.166, p<0.05 vs FI =5.58, df=1.289, p<0.05) and AE (FD =11.63, df=1.201, p<0.05 vs. FI =3.035, df=1.32, p>0.05) is exercise duration. Conclusions These findings suggest that exercise intensity was an essential factor of acute oxidative stress and antioxidant enzyme responses compared with the duration of exercise.

scholarly journals Alfacalcidol modulates oxidative stress parameters in the peripheral blood of patients with active rheumatoid arthritis

2016 ◽  
Vol 81 (10) ◽  
pp. 1127-1139 ◽  
Author(s):  
Tatjana Zivanovic-Radnic ◽  
Katarina Simic-Pasalic ◽  
Mirjana Sefik-Bukilica ◽  
Sonja Misirlic-Dencic ◽  
Andjelka Isakovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Das28 Score ◽  
Sod Activity ◽  
Oxidative Stress Parameters ◽  
Before And After ◽  
Significant Clinical Improvement ◽  
Membrane Depolarisation ◽  
O2 Production ◽  
Gpx Activity ◽  
Stress Parameters

Hormone D and its analogues display immunomodulatory activities providing a beneficial effect in immunoinflammatory diseases. The aim of this study was to assess the effect of alfacalcidol treatment on superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity and glutathione (GSH) and malondialdehyde (MDA) levels in patients with active RA. Sixteen patients with active RA and twenty controls were enrolled in the study. Blood samples were taken before and after 12 weeks of alfacalcidol therapy (2 ?g/day). Oxidative stress parameters were determined spectrophotometrically and by flow cytometry assessment. Disease activity was assessed using DAS28 score. The results revealed that alfacalcidol treatment, significantly (p = 0.04) reduced SOD activity and CAT activity (p = 0.001) in RA patients. The activity of GPx was significantly lower in RA patients before treatment, compared to controls (p = 0.04). After therapy, GPx activity was restored to control levels, and GSH levels were significantly reduced (p = 0.01). MDA levels in patients at the beginning of the study protocol, remained significantly elevated compared to controls (p = 0.01). Alfacalcidol treatment decreased MDA levels in patients (p = 0.19). Furthermore, 12-weeks alfacalcidol therapy, changed the response of RA patients? PBMC to stimulation preventing the O2 - production and mitochondrial membrane depolarisation. After alfacalcidol treatment, significant clinical improvement was observed.

Oxidative stress as a mechanism of combined OTA and CTN toxicity in rat plasma, liver and kidney

2018 ◽  
Vol 38 (4) ◽  
pp. 434-445 ◽  
Author(s):  
D Rašić ◽  
V Micek ◽  
MS Klarić ◽  
M Peraica
Keyword(s):  
Oxidative Stress ◽  
Rat Plasma ◽  
Protein Carbonyl ◽  
Protein Carbonyls ◽  
Lower Plasma ◽  
Sod Activity ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Gpx Activity

Ochratoxin A (OTA) and citrinin (CTN) commonly coexist in grains. Aiming to evaluate oxidative stress in OTA + CTN toxicity, male Wistar rats were orally treated with two doses of OTA (0.125 and 0.250 mg kg−1 of body weight (b.w.)), CTN (2 mg kg−1 of b.w.) and resveratrol (RSV; 20 mg kg−1 of b.w.) and combined daily during 3 weeks. Protein carbonyl concentrations were measured in kidneys and liver; catalytic activity of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) level in plasma, kidneys and liver, while malondialdehyde (MDA) concentration was measured in plasma, kidneys, liver and urine. Mycotoxin treatment significantly increased MDA concentration in plasma and kidney and decreased SOD activity in the liver. Rats treated with CTN and OTA125 + CTN had lower plasma GPx activity. Concentration of GSH in the kidney and protein carbonyls in the kidney and liver as well as GPx activity in the kidney and liver, SOD activity in the kidney and CAT activity in the liver were not affected. Protective effect of RSV was observed on GSH in the kidney and plasma and MDA in the kidney, plasma and urine. Oxidative stress is involved in OTA + CTN toxicity in vivo because such treatment affects parameters of oxidative stress, particularly in plasma. RSV can reduce but not overcome oxidative stress induced by combined OTA and CTN treatment.

Factors associated with oxidative stress status in pediatric patients with type 1 diabetes mellitus

2020 ◽  
Vol 33 (5) ◽  
pp. 591-598
Author(s):  
Dragana Kacarevic ◽  
Natasa Bogavac-Stanojevic ◽  
Vesna Spasojevic-Kalimanovska ◽  
Dragana Bojanin ◽  
Tatjana Milenkovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Weight Status ◽  
Pediatric Patients ◽  
Hdl Cholesterol ◽  
Antioxidative Defense ◽  
Sod Activity ◽  
Oxidative Stress Status

AbstractBackgroundOxidative stress is implicated in both, the onset and the progression of type 1 diabetes mellitus (T1DM). There is accumulated evidence of increased biomarkers of oxidative stress in newly diagnosed, T1DM patients without complications, and in those with advanced disease. In this cross-sectional study, we investigated factors affecting oxidative stress status in pediatric patients with T1DM.MethodsAdvanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB), total sulfhydryl (SH) groups, and superoxide dismutase (SOD) activity were determined in 170 children and adolescents with T1DM. Principal component analysis was used to investigate clustering of clinical and laboratory variables associated with elevated oxidative stress and reduced antioxidative defense biomarkers.ResultsFactor analysis extracted five factors, interpreted as (1) “weight status factor” including age, BMI, waist and hip circumferences; (2) “proatherogenic factor” that included LDL-cholesterol, non-HDL-cholesterol, and triglycerides; (3) “metabolic control factor” including glucose and HbA1c; (4) “renal marker factor” with positive loading of urinary albumin excretion rate and negative loading of GFR; and (5) “antiatherogenic factor” that included HDL-cholesterol. High AOPP levels were independently predicted by “proatherogenic” (OR: 2.32; 95% CI: 1.44–3.71; p < 0.001), “metabolic control” (OR: 2.24; 95% CI: 1.35–3.73; p < 0.01), and “renal marker” (OR: 1.65; 95% CI: 1.03–2.65; p < 0.05) factors. “Renal marker factor” was a significant predictor of PAB (OR: 0.52; 95% CI: 0.34–0.81; p < 0.01). Regarding antioxidative defense markers, reduced SH groups were predicted by “proatherogenic factor” (OR: 0.56; 95% CI: 0.34–0.94; p < 0.05), while “weight status factor” predicted lower SOD activity (OR: 1.66; 95% CI: 1.03–2.67; p < 0.05).ConclusionsCardiometabolic risk factors and renal function are associated with oxidative stress in pediatric T1DM patients.

Sign in / Sign up

Export Citation Format

Share Document