Quercetin ameliorates liver injury induced with Tripterygium glycosides by reducing oxidative stress and inflammation

2015 ◽  
Vol 93 (6) ◽  
pp. 427-433 ◽  
Author(s):  
Junming Wang ◽  
Mingsan Miao ◽  
Yueyue Zhang ◽  
Ruixin Liu ◽  
Xaobing Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Inflammatory Cytokine ◽  
Ethanol Extract ◽  
Serum Levels ◽  
Positive Control ◽  
Gamma Glutamyl Transferase ◽  
Necrosis Factor Alpha ◽  
Anti Inflammatory ◽  
Glutamyl Transferase

Quercetin (Que) is one of main compounds in Lysimachia christinae Hance (Christina loosestrife), and has both medicinal and nutritional value. Glycosides from Tripterygium wilfordii Hook.f. (léi gōng téng [the thunder duke vine]; TG) have diverse and broad bioactivities but with a high incidence of liver injury. Our previous study reported on the hepatoprotective properties of an ethanol extract from L. christinae against TG-induced liver injury in mice. This research is designed to observe, for the first time, the possible protective properties of the compound Que against TG-induced liver injury, and the underlying mechanisms that are involved in oxidative stress and anti-inflammation. The results indicated that TG caused excessive elevation in serum levels of alanine/aspartate transaminase (ALT/AST), alkaline phosphatase (ALP), gamma glutamyl transferase (γ-GT), and pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α), as well as hepatic lipid peroxidation (all P < 0.01). On the other hand, following TG exposure, we observed significantly reduced levels of biomarkers, including hepatic glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxidase (GPx), and the anti-inflammatory cytokine interleukin (IL)-10, as well as the enzyme activity and mRNA expression of copper- and zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) (all P < 0.01). Nevertheless, all of these alterations were reversed by the pre-administration of Que or the drug bifendate (positive control) for 7 consecutive days. Therefore, this study suggests that Que ameliorates TG-induced acute liver injury, probably through its ability to reduce oxidative stress and its anti-inflammatory properties.

scholarly journals Anti-Inflammatory, Immunomodulatory, and Antioxidant Activities of Allicin, Norfloxacin, or Their Combination againstPasteurella multocidaInfection in Male New Zealand Rabbits

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Rasha T. M. Alam ◽  
Elshaima M. Fawzi ◽  
Maha I. Alkhalf ◽  
Wafa S. Alansari ◽  
Lotfi Aleya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
New Zealand ◽  
Inflammatory Cytokine ◽  
Pasteurella Multocida ◽  
Antioxidant Activities ◽  
Hypochromic Anemia ◽  
Serum Levels ◽  
Type B ◽  
Anti Inflammatory ◽  
New Zealand Rabbits

The present study investigated the efficacy of allicin as an antibacterial, anti-inflammatory, antioxidant, and immunostimulant agent in reducing the severity ofPasteurella multocida(P. multocida) type B infection in rabbits. Fifty New Zealand rabbits, 5 weeks old, were divided equally into five groups. Except for group 1, all groups were intranasally infected withP. multocidatype B (2 × 105colony forming units/ml/rabbit). Then, group 3 rabbits were orally treated with allicin (50 mg/kg BW) for 5 days, group 4 rabbits received a single oral dose of norfloxacin 30% (100 mg/kg BW), while group 5 rabbits were treated with a combination of norfloxacin and allicin. Hematological, serum biochemical, inflammatory cytokine, immunological, and histopathological analyses were performed. Results revealed that rabbits, infected withP. multocidatype B, exhibited macrocytic hypochromic anemia and leukocytosis with a significant elevation in the phagocytic percentage and index. Moreover, significant reductions in serum total protein, albumin, globulin, and immunoglobulin (IgG and IgM) levels were observed in infected rabbits. Infected rabbits showed significant increases in serum inflammatory cytokine (TNF-αand IL-6), alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, and serum bilirubin (total, direct, and indirect) levels. Further,P. multocidainfection induced oxidative stress as demonstrated by the significant reduction in serum levels of reduced glutathione and superoxide dismutase enzyme and marked elevation in serum malondialdehyde. Treatment with allicin, norfloxacin, or their combination significantly ameliorated the alterations in all studied parameters. In conclusion, allicin could ameliorate the inflammation and oxidative stress, induced byP. multocidatype B infection in rabbits.

scholarly journals Toxicity and Anti-inflammatory Activities of an Extract of the Eleutherine bulbosa Rhizome on Collagen Antibody-induced Arthritis in a Mouse Model

2018 ◽  
Vol 13 (7) ◽  
pp. 1934578X1801300
Author(s):  
Pham Thi Bich Hanh ◽  
Do Thi Thao ◽  
Nguyen Thi Nga ◽  
Ngo Thi Phuong ◽  
Le Ngoc Hung ◽  
...  
Keyword(s):  
Mouse Model ◽  
Ethanol Extract ◽  
Serum Levels ◽  
Negative Control ◽  
High Dose ◽  
Necrosis Factor Alpha ◽  
Beneficial Effects ◽  
Anti Inflammatory

As a continuation of our interest in the anti-inflammatory activities of Vietnamese plants, we searched for novel anti-inflammatory agents in Eleutherine bulbosa and evaluated the anti-inflammatory effects of an ethanol extract of the rhizome of E. bulbosa (EBE) on lipopolysaccharide-stimulated RAW 264.7 macrophages in vitro and in a collagen antibody-induced arthritic (CAIA) mouse model in vivo. Treatment of the CAIA mice with EBE decreased the incidence of arthritis, especially at a dose of 1000 mg/kg body weight. A significant ( P<0.05) decrease in the arthritis score was seen after high-dose EBE treatment between days 10 and 14 in comparison with the negative control. The serum levels of the inflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, and IL-10 in the mice were measured using commercial ELISA kits. The results suggest that an ethanol extract of the E. bulbosa rhizome has beneficial effects on inflammatory cytokine regulation in an experimental CAIA model.

scholarly journals Lavender (Lavandula officinalis) essential oil prevents acetaminophen-induced hepatotoxicity by decreasing oxidative stress and inflammatory response

2021 ◽  
Vol 10 (3) ◽  
pp. e43410313461
Author(s):  
Gabriel Fernando Esteves Cardia ◽  
Francielli Maria de Souza Silva-Comar ◽  
Expedito Leite Silva ◽  
Edvalkia Magna Teobaldo da Rocha ◽  
Jurandir Fernando Comar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Essential Oil ◽  
Serum Levels ◽  
Gamma Glutamyl Transferase ◽  
Once Daily ◽  
Underlying Mechanisms ◽  
Lavandula Officinalis ◽  
Glutamyl Transferase ◽  
Therapeutic Doses ◽  
Pro Inflammatory Cytokines

Acetaminophen (N-acetyl-p-aminophenol, APAP) is the most popular drug recommended and consumed for relieving mild and moderate pain and fever. Although effective in therapeutic doses, APAP overdose induces hepatotoxicity, causing acute liver failure. In this study, the hepatoprotective effects and the underlying mechanisms of Lavandula officinalis essential oil (LEO) were investigated in APAP-induced hepatotoxicity. To evaluate the hepatoprotective effect, Balb /c mice were pretreated with LEO at doses of 200 and 400 mg/kg, once daily for seven days. On the seventh day, mice were treated with APAP (250 mg/kg) to induce hepatotoxicity. LEO significantly decreased serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (γ-GT) compared to the APAP group. Besides, a decrease in myeloperoxidase (MPO) activity, nitric oxide (NO), and pro-inflammatory cytokines levels were observed in liver samples of the LEO treated mice. Moreover, pretreatment with LEO showed significant antioxidant activity by decreasing the levels of malondialdehyde (MDA), carbonylated proteins, reactive oxygen species (ROS), and glutathione (GSH), in addition to increasing levels of the hepatic superoxide dismutase (SOD), catalase (CAT), and oxidized glutathione (GSSG). Our results showed that LEO improved liver functions altered by APAP by inhibiting oxidative stress and inflammatory induced by APAP and other oxidative stress-mediated toxicities.

scholarly journals Effect of Tannin-Rich Extract of Chasmanthera dependens on Piroxicam-induced Liver Damage in Male Wistar Rats

2021 ◽  
Vol 5 (1) ◽  
pp. 27
Author(s):  
Tijani Stephanie Abiola ◽  
Olori Ogaraya David ◽  
Farombi Ebenezer Olatunde
Keyword(s):  
Oxidative Stress ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Significant Rise ◽  
Control Group ◽  
Gamma Glutamyl Transferase ◽  
Male Wistar Rats ◽  
Anti Inflammatory ◽  
Group 2 ◽  
Glutamyl Transferase

Background: Piroxicam is one of the nonsteroidal anti-inflammatory drugs used as antipyretic, analgesic and anti-inflammatory drug often used for the relief of nonspecific fever condition and in arthritis. This study investigated the protective potential of tannin-rich extract of Chasmanthera dependens (TRECDS) against piroxicam-induced hepatotoxicity in male Wistar rats.Materials and Methods: Thirty two rats were divided into four groups. Group 1 received normal saline and served as the control group, group 2 were given 20 mg/kg piroxicam only, while groups 3 and 4 were given 20 mg/kg piroxicam with the addition of 200 and 400 mg/kg of tannin-rich extract of Chasmanthera dependens, respectively. All rats were treated orally once daily for ten days.Results: Administration of piroxicam caused liver atrophy demonstrated by significant rise in serum alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), glucose-6-phosphate dehydrogenase (G6PDH) levels of albumin (ALB), bilirubin (BIL), total cholesterol (TCHOL), triglyceride (TRIGS) and low-density lipoprotein (LDL). Piroxicam also decreased high-density lipoprotein (HDL) level, enzymatic and nonenzymatic antioxidant levels significantly (p>0.05) with attendant increase in oxidative stress indices in the liver of rats compared with control group. Histological assessment reveled severe damaged to the liver of rats. However, co-administration with TRECDS reversed these observations as evidenced in the histological results.Conclusion: The findings of this study showed that exposure of rats to piroxicam provoked damage to the liver via oxidative damage and TRECDS has the potential of ameliorating the damage.Keywords: hepatotoxicity, piroxicam, Chasmanthera dependens, oxidative stress

UJI AKTIVITAS HEPATOPROTEKTOR KOMBINASI EKSTRAK ETANOL DAUN PANDAN WANGI (Pandanus amaryllifolius roxb) DAN KAYU MANIS (Cinnamomum burmanii) TERHADAP KADAR MDA YANG DIINDUKSI PARASETAMOL

2020 ◽  
pp. 68-73
Author(s):  
Yuni Asri Mulatsih Agami ◽  
Eka Wisnu Kusuma
Keyword(s):  
Oxidative Stress ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Experimental Methods ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Negative Group ◽  
Ic50 Value ◽  
Dose Combination

Kasus penyakit hati semakin meningkat seiring penggunaan senyawa hepatotoksin salah satunya karena penggunaan parasetamol dengan dosis berlebih. Hal tersebut dapat meningkatkan produksi radikal bebas sehingga memicu terjadinya stress oksidatif yang dapat menimbulkan kerusakan jaringan yang ditandai dengan peningkatan kadar Malondialdehyde (MDA). Stress oksidatif dapat diatasi dengan antioksidan dari berbagai tanaman. Kulit kayu manis memiliki aktivitas antioksidan dengan nilai IC50 53ppm dan daun pandan wangi 39,7%  Penelitian ini bertujuan untuk mengetahui aktivitas kombinasi ekstrak etanol daun pandan wangi dan kayu manis dalam menurunkan kadar MDA. tikus yang diinduksi parasetamol. Penelitian menggunakan metode eksperimental, dilakukan selama 9 hari dengan 30 ekor tikus jantan dibagi menjadi 6 Kelompok, yaitu: Normal diberi aquadest, Kontrol Positif diberi silimarin 100 mg/kgBB, Kontrol Negatif diberi CMC-Na 0,05%, serta 3 kelompok lainnya diberi kombinasi ekstrak daun pandan wangi:kayu manis berturut-turut dosis I (25:75), dosis II (50:50), dosis III (75:25). Semua kelompok diinduksi parasetamol 2,5 g/kgBB pada hari ke-7  setelah 30 menit perlakuan, kecuali kelompok normal. Pada hari ke 9 dilakukan pengukuran kadar MDA dengan metode TBARs menggunakan spektrofotometri. Pemberian kombinasi ekstrak etanol daun pandan wangi dan kayu manis dapat menurunkan kadar MDA dengan kombinasi dosis yang paling optimal adalah 75:25 berdasarkan statistik dengan nilai signifikan 0,000<0,05 dibandingkan dengan kelompok negatif.    Cases of liver disease have increased with the use of hepatotoxin compounds, one of which is due to the use of paracetamol with excessive doses. This can increase the production of free radicals so that it triggers oxidative stress which can cause tissue damage which is characterized by increased levels of Malondialdehyde (MDA). Oxidative stress can be overcome with antioxidants from various plants. Cinnamomum burmanii has antioxidant activity with IC50 value of 53ppm and Pandanus amarrylifolius 39.7%. This study aims to determine the combined activity of ethanol extract of Pandanus amarrylifolius and Cinnamomum burmanii  in reducing MDA levels. Paracetamol-induced rats. Research using experimental methods, conducted for 9 days with 30 male rats divided into 6 groups, namely: Normal given aquadest, Positive Control were given silimarin 100 mg / kgBB, Negative Control was given CMC-Na 0.05%, and 3 other groups were given a combination of Pandanus amarrylifolius extract: Cinnamomum burmanii dose I (25:75), dose II (50:50), dose III (75:25). All groups induced paracetamol 2.5 g / kgBB on the 7th day after 30 minutes of treatment, except the normal group. On the 9th day MDA levels were measured using the TBARs method using spectrophotometry. Giving a combination of Pandanus amarrylifolius and Cinnamomum burmanii ethanol extract can reduce MDA levels with the most optimal dose combination is 75:25 based on statistics with a significant value of 0,000<0.05 compared with the negative group.

Interactions Between Dental Composite Resins and Saliva A comparative biochemical in vitro study

2019 ◽  
Vol 56 (3) ◽  
pp. 529-533
Author(s):  
Mihaela Pantea ◽  
Diana Andreea Ighigeanu ◽  
Alexandra Totan ◽  
Maria Greabu ◽  
Daniela Miricescu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Study ◽  
Composite Resins ◽  
Vitro Study ◽  
Dental Composite ◽  
Gamma Glutamyl Transferase ◽  
Specific Protocol ◽  
Dental Composite Resins ◽  
Glutamyl Transferase

This in vitro study analyses the biochemical interaction between saliva and three types of dental composite resins (a direct resin, an indirect resin and a dual-cure resin used for cementation of indirect dental restorations). The resin samples were obtained following a specific protocol and in line with the producers� recommendations; the resin samples were incubated with saliva samples collected from 19 healthy volunteers. The obtained results showed that the tested composite resins did not produce significant changes in oxidative stress parameters that were analysed (albumin, uric acid, GGT / gamma glutamyl transferase, OXSR-1 / oxidative stress responsive kinase 1) and do not influence the inflammatory salivary status reflected by the levels of IL-6 - an inflammatory marker.

scholarly journals Dipterocarpus tuberculatus Roxb. Ethanol Extract Has Anti-Inflammatory and Hepatoprotective Effects In Vitro and In Vivo by Targeting the IRAK1/AP-1 Pathway

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2529
Author(s):  
Haeyeop Kim ◽  
Woo Seok Yang ◽  
Khin Myo Htwe ◽  
Mi-Nam Lee ◽  
Young-Dong Kim ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Related Proteins ◽  
Pro Inflammatory Cytokines ◽  
Inflammatory Effect

Dipterocarpus tuberculatus Roxb. has been used traditionally as a remedy for many diseases, especially inflammation. Therefore, we analyzed and explored the mechanism of the anti-inflammatory effect of a Dipterocarpus tuberculatus Roxb. ethanol extract (Dt-EE). Dt-EE clearly and dose-dependently inhibited the expression of pro-inflammatory cytokines such as IL-6, TNF-α, and IL-1β in lipopolysaccharide (LPS)-treated RAW264.7 cells. Also, Dt-EE suppressed the activation of the MyD88/TRIF-mediated AP-1 pathway and the AP-1 pathway related proteins JNK2, MKK4/7, and TAK1, which occurred as a result of inhibiting the kinase activity of IRAK1 and IRAK4, the most upstream factors of the AP-1 pathway. Finally, Dt-EE displayed hepatoprotective activity in a mouse model of hepatitis induced with LPS/D-galactosamine (D-GalN) through decreasing the serum levels of alanine aminotransferase and suppressing the activation of JNK and IRAK1. Therefore, our results strongly suggest that Dt-EE could be a candidate anti-inflammatory herbal medicine with IRAK1/AP-1 inhibitory and hepatoprotective properties.

scholarly journals Allopurinol ameliorates liver injury in type 1 diabetic rats through activating Nrf2

2021 ◽  
Vol 35 ◽  
pp. 205873842110314
Author(s):  
Fei Zeng ◽  
Jierong Luo ◽  
Hong Han ◽  
Wenjie Xie ◽  
Lingzhi Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Caspase 3 ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Heme Oxygenase 1 ◽  
Liver Protein ◽  
Lipid Peroxidation Product ◽  
Protein Expressions

Hyperglycemia-induced oxidative stress plays important roles in the development of non-alcoholic fatty liver disease (NAFLD), which is a common complication in diabetic patients. The Nrf2-Keap1 pathway is important for cell antioxidant protection, while its role in exogenous antioxidant mediated protection against NAFLD is unclear. We thus, postulated that antioxidant treatment with allopurinol (ALP) may attenuate diabetic liver injury and explored the underlying mechanisms. Control (C) and streptozotocin (STZ)-induced diabetes rats (D) were untreated or treated with ALP for 4 weeks starting at 1 week after diabetes induction. Serum levels of alanine aminotransferase (ALT) and aspartate transaminase (AST), production of lipid peroxidation product malondialdehyde (MDA), and serum superoxide dismutase (SOD) were detected. Liver protein expressions of cleaved-caspase 3, IL-1β, nuclear factor-erythroid-2-related factor-2 (Nrf2), heme oxygenase-1 (HO-1), P62, Kelch-like ECH-associated protein 1 (Keap1), and LC3 were analyzed. In vitro, cultured rat normal hepatocytes BRL-3A were grouped to normal glucose (5.5 mM, NG) or high glucose (25 mM, HG) and treated with or without allopurinol (100 µM) for 48 h. Rats in the D group demonstrated liver injury evidenced as increased serum levels of ALT and AST. Diabetes increased apoptotic cell death, enhanced liver protein expressions of cleaved-caspase 3 and IL-1β with concomitantly increased production of MDA while serum SOD content was significantly reduced (all P < 0.05 vs C). In the meantime, protein levels of Nrf2, HO-1, and P62 were reduced while Keap1 and LC3 were increased in the untreated D group as compared to control ( P < 0.05 vs C). And all the above alterations were significantly attenuated by ALP. Similar to our findings obtained from in vivo study, we got the same results in in vitro experiments. It is concluded that ALP activates the Nrf2/p62 pathway to ameliorate oxidative stress and liver injury in diabetic rats.

scholarly journals Effect of ethanol extract of leaf of Cajanus indicus spreng in carbon tetrachloride induced hepatopathy in rates in relation to free radical scavenging action

2012 ◽  
Vol 1 (1) ◽  
pp. 20-26
Author(s):  
Biswajit Majumdar ◽  
Arun Kumar Sinha ◽  
Shrawan Yadav
Keyword(s):  
Hepatic Injury ◽  
Glutathione Transferase ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Conjugate Diene ◽  
Gamma Glutamyl Transferase ◽  
Asian Countries ◽  
Medical College ◽  
Glutathione Status ◽  
Glutamyl Transferase

Phytochemicals, that is, chemicals present in various plants and herbs , are now becoming important candidates for development of drugs.Wide range of medicinals plants {Plants from which potential photochemicals are isolated for development of drugs for treatment of diseases}present in South Asian countries have now been increasingly utilized for development of phytomedicines. Treatment with ethanol extract of leaf of Cajanus indicus Spreng at a dose of 50 mg /kg body weight for 20 days, after induction of hepatotoxic damage by CCl4, produce significant elevation of the hepatic injury. The liver marker enzymes like(Aspartate Transaminate) AST, GGT(Gamma Glutamyl Transferase), ALT(Alanine Transaminase) and ALP(Alkaline Phosphatase) decreased significantly at the above dose showing the optimum effect against hepatic damage. The liver antioxidant enzymes SOD, catalase, glutathione peroxidase, glutathione reductase and glutathione transferase and the membrane damaging indicators TBARS(Thiobarbituric Acis Reactive Species), conjugate diene and marker of glutathione status indicate the mechanism of healing action to be due to scavenging of free radicals or ROS. The results thus gives a confirmatory proof that the healing action of ethanol extract of leaf of Cajanus indicus Spreng is for shifting of equilibrium from the peroxidant to antioxidant side and the leaf acts as a natural antioxidant and healer of CCl4 induced hepatotoxicity.DOI: http://dx.doi.org/10.3126/jonmc.v1i1.7284 Journal of Nobel Medical College Vol.1(1) 2011 20-26

scholarly journals In Vitro Anti-Inflammatory Assay of Bitter Melon (Momordica charantia L.) Ethanol Extract

2020 ◽  
Vol 7 (3) ◽  
pp. 1-4
Author(s):  
Rahmawati Rahmawati ◽  
Harti Widiastuti ◽  
Eka Sulistya
Keyword(s):  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Positive Control ◽  
Regression Equation ◽  
Positive Control Group ◽  
Control Group ◽  
Bitter Melon ◽  
Inflammatory Agent ◽  
Anti Inflammatory

Bitter melon contains flavonoids that have anti-inflammatory function. Inflammation can be caused by protein denaturation. This research tested the anti-inflammatory potential of ethanol extract of leaves and bitter melon (Momordica charantia L.) using a UV-Vis spectrophotometer and protein denaturation inhibition method. There were three experimental groups formed in this research including negative control group, positive control group, and test solutions. Diclofenac sodium was used in the positive control group at concentration series of 1, 2, 4, 8, and 16 ppm, obtaining a regression equation Y = 3.546X + 2.163 and r = 0.9990. Whilst, for bitter melon ethanol extract at a series of concentrations of 10, 20, 40, 80 and 160 ppm obtained a regression equation Y = 0.243X + 11.74 and r = 0.9995. The potential of diclofenac sodium as an anti-inflammatory agent was shown by IC50 of 13.490 µg / mL, while the ethanol extract of bitter melon fruit has an IC50 of 157.448 µg / mL. This result indicated that bitter melon ethanol extract had the potential as moderate anti-inflammatory agent.

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