Duodenal application of Li+ in a submaximal therapeutic dose inhibits exocrine pancreatic secretion and modulates gastro-duodenal myoelectrical activity in a conscious pig model

2013 ◽  
Vol 91 (9) ◽  
pp. 764-772
Author(s):  
Violetta Naughton ◽  
Mette S. Hedemann ◽  
Patrick J. Naughton ◽  
Emeir McSorley ◽  
Helle N. Laerke
Keyword(s):  
Pancreatic Juice ◽  
Recovery Period ◽  
Pancreatic Enzyme ◽  
Smooth Muscles ◽  
Phase Iii ◽  
Myoelectrical Activity ◽  
Intraduodenal Infusion ◽  
Activity Phase ◽  
Gastroduodenal Motility ◽  
Bicarbonate Output

This study tested whether duodenal application of lithium inhibits gastroduodenal motility, and whether it suppresses secretion from the exocrine pancreas. Five suckling pigs, 16–18 days old, were surgically fitted with 3 serosal electrodes on the wall of the gastric antrum and the duodenum for electromyography of smooth muscles, and with a pancreatic duct catheter and a duodenal T-cannula for collection and re-entrant flow of pancreatic juice. After the recovery period, on alternative days, each animal was tested once with an intraduodenal infusion of Li+ (100 mmol·L–1 C3H5LiO3, 10 mL·kg−1·h−1) for 1 h, and once with an intraduodenal infusion of NaCl (154 mM, 10 ml·kg−1·h−1), also for 1 h, with the first treatment, i.e., Li+ or NaCl, randomly assigned. Individual pigs served as their own controls, with data recorded prior to a treatment being used as the baseline. Li+ increased the duration of quiescence (P < 0.05) and activity phase (P < 0.05) in the antrum, thus increasing (P < 0.05) the duration of antral myoelectrical cycles. Li+ shortened (P < 0.05) phase I, but it did not affect phase II or phase III or the MMC in the duodenum. Li+ inhibited pancreatic juice outflow as well as pancreatic enzyme and bicarbonate output (P < 0.05 for all pancreatic parameters).

Mechanisms behind changes in gastric acid and bicarbonate outputs during the human interdigestive motility cycle

1996 ◽  
Vol 270 (1) ◽  
pp. G113-G122 ◽  
Author(s):  
J. Dalenback ◽  
L. Fandriks ◽  
L. Olbe ◽  
H. Sjovall
Keyword(s):  
Acid Secretion ◽  
Duodenogastric Reflux ◽  
Phase Iii ◽  
Maximum Output ◽  
Motor Complex ◽  
Gastroduodenal Motility ◽  
Bicarbonate Output ◽  
Interdigestive Motility ◽  
Motility Cycle ◽  
Stimulation Of

Human gastric interdigestive acid and bicarbonate outputs vary cyclically in association with the migrating motor complex (MMC). These phenomena were studied in 26 healthy volunteers by constant-flow gastric perfusion, with continuous recording of pH and Pco2 in mixed gastric effluent and concomitant open-tip manometry of gastroduodenal motility. Stable acid and bicarbonate outputs were registered during less than 50% of the MMC cycle. Acid secretion started to increase 71 +/- 3% into the cycle, with maximum output during antral phase III. Bicarbonate output increased biphasically 1) 40 +/- 5% into the cycle, coinciding with reflux of bile, and 2) at the end of duodenal phase III when the aspirate was devoid of bile. The bicarbonate peak associated with phase III was abolished by atropine (0.01 mg/kg iv, n = 8) and by pyloric occlusion (n = 9) but remained unchanged after omeprazole (n = 10). The acid peak was abolished by both atropine and omeprazole. It is concluded that the MMC-related changes in acid and alkaline outputs represent two different and independent phenomena. Acid secretion cyclicity is due to periodical variations in cholinergic stimulation of the parietal cells. In contrast, the phase III-associated increase in bicarbonate output is due to duodenogastric reflux.

Release of cholecystokinin and secretin by sodium oleate in dogs: molecular form and bioactivity

1992 ◽  
Vol 262 (1) ◽  
pp. G35-G43 ◽  
Author(s):  
G. Sun ◽  
T. M. Chang ◽  
W. J. Xue ◽  
J. F. Wey ◽  
K. Y. Lee ◽  
...  
Keyword(s):  
Pancreatic Juice ◽  
Sodium Oleate ◽  
Molecular Form ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
Conscious Dogs ◽  
Oral Ingestion ◽  
Intraduodenal Infusion ◽  
Predominant Form ◽  
Duodenal Lumen

The release of cholecystokinin (CCK) and secretin into both circulation and duodenal lumen, after intraduodenal perfusion with sodium oleate or oral ingestion of fat, was studied in anesthetized and conscious dogs, respectively. Intraduodenal infusion with sodium oleate (4 mmol.kg.-1.h-1, pH 9.5) in anesthetized dogs with diversion of bile and pancreatic juice stimulated the release of both CCK and secretin not only into the circulation but also into the duodenal lumen. The concentration of CCK and secretin in the luminal perfusate increased from 0.2 +/- 0.1 to 2.1 +/- 0.4 nM and 0.34 +/- 0.16 to 2.59 +/- 0.63 nM, respectively. Intraduodenal infusion of NaHCO3 solution at pH 9.5 did not result in release of either hormone. Luminal release of both hormones was also observed by intraduodenal infusion of sodium oleate in the dogs without diversion of bile and pancreatic juice, albeit at lower concentrations than those released in the dogs with diversion. Analysis of the molecular form of luminal secretin by gel filtration, ion-exchange chromatography, and high-performance liquid chromatography showed only a single form of secretin with molecular size, hydrophobicity, and charge similar to those of natural porcine secretin. In contrast, multiple forms of CCK were released into both circulation and duodenal lumen with CCK-58 as the predominant form. In conscious dogs, CCK-58 was also found to be the predominant form of CCK released into the circulation after oral ingestion of fat.(ABSTRACT TRUNCATED AT 250 WORDS)

LAPAROSCOPIC SANTULLI INTESTINAL ANASTOMOSIS IN AN INFANT WITH CYSTIC FIBROSIS

2021 ◽  
Vol 100 (6) ◽  
pp. 187-191
Author(s):  
Yu.A. Kozlov ◽  
◽  
L.V. Bregel ◽  
A.A. Rasputin ◽  
P.J. Baradieva ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Postoperative Period ◽  
Intestinal Anastomosis ◽  
Recovery Period ◽  
Pancreatic Enzyme ◽  
Electrolyte Imbalance ◽  
Fluid Loss ◽  
Enzyme Replacement ◽  
Age Related ◽  
Full Diet

The aim of the study was to evaluate the technical feasibility of performing a Santulli-type side-toend inter-intestinal anastomosis in infants with cystic fibrosis (CF) using laparoscopy. A clinical case of a 3-month-old child with CF who was operated on in the neonatal period for meconium ileus is presented. The baby's gestational age was 32 weeks. The primary operation consisted of the formation of a terminal enterostomy. In the postoperative period, a malabsorption syndrome was noted, accompanied by a lack of growth. As a temporary measure to restore nutritional status, a Santulli-style side-to-end laparoscopic intestinal anastomosis was performed. The observed child was found to have the F508del mutation in both alleles of the CFTR gene. The patient's weight at the time of the Santulli anastomosis construction was 2900 g, the age – 3 months. During the operation, there were no difficulties associated with the mobilization of the separated segments of the ileum. The duration of the surgical intervention was 70 min. Enteral nutrition was started on the 3rd day after the operation. The recovery period for intestinal transit through the rectum was 15 days. The postoperative period was uneventful. The duration of hospitalization was 18 days. No electrolyte imbalance or excessive fluid loss or underweight associated with enterostomy was observed. Over the next 6 months, the normalization of age-related weight and height parameters was achieved, after which the continuity of the digestive tract was restored by closing the terminal enterostomy. Currently, the patient has minimal respiratory symptoms and is receiving adequate CF therapy with pancreatic enzyme replacement therapy included with each meal. The initial experience of performing laparoscopic Santulli inter-intestinal anastomosis in an infant with CF presented in the study showed the possibility and reproducibility of this technique, expanding the boundaries of laparoscopy in pediatric practice. The decision to close the enterostomy in these patients should be deferred until a full diet is introduced and should be made in conjunction with a pediatrician specializing in the treatment of CF.

Maximum alkaline (bicarbonate) output of the dog pancreas

1963 ◽  
Vol 204 (2) ◽  
pp. 251-256 ◽  
Author(s):  
J. H. Baron ◽  
C. V. Perrier ◽  
H. D. Janowitz ◽  
D. A. Dreiling
Keyword(s):  
Pancreatic Juice ◽  
Chloride Concentration ◽  
Repeated Measurements ◽  
Maximum Output ◽  
Bicarbonate Concentration ◽  
Response Curves ◽  
Intravenous Injections ◽  
Intravenous Infusions ◽  
Dog Pancreas ◽  
Bicarbonate Output

By cannulation of the major pancreatic duct in six dogs with chronic Thomas type duodenal fistulas, dose-response curves have been obtained for the volume and bicarbonate output of pancreatic secretion stimulated by secretin (Vitrum). With single rapid intravenous injections (0.5–15 U/kg) or continuous intravenous infusions (1–16 U/min), increased doses of secretin produced increased volumes of pancreatic juice and bicarbonate outputs up to a maximum. This maximum alkaline output of the pancreas was reached at doses ranging from 7.5– 12.5 U/kg for single injections, and 4–16 U/min for infusions. In each dog supramaximum doses led to a fall in juice volume and bicarbonate output, unaccompanied by side effects. With supramaximum doses or intravenous infusions lasting longer than 30–90 min, bicarbonate concentration fell independently of juice volume. The chloride concentration consistently varied inversely with bicarbonate concentration. The sodium, potassium, and amylase concentrations were unaffected by rate of flow. Repeated measurements of basal secretion showed considerable variation. Coefficients of variation were low for repeated measurements of secretin-stimulated secretion, the most repeatable measurement being the maximum output of pancreatic juice in the first 15 min after a single injection which, in each of the six dogs, was almost identical to the plateau 15 min during infusion.

scholarly journals Action of secretin on pancreatic enzyme secretion in man. Studies on pure pancreatic juice.

Gut ◽  
1984 ◽  
Vol 25 (8) ◽  
pp. 867-873 ◽  
Author(s):  
L Gullo ◽  
P Priori ◽  
P L Costa ◽  
G Mattioli ◽  
G Labo
Keyword(s):  
Pancreatic Juice ◽  
Pancreatic Enzyme ◽  
Enzyme Secretion ◽  
Pure Pancreatic Juice ◽  
Pancreatic Enzyme Secretion

scholarly journals Regulation of Output of Electrolytes in Bile and Pancreatic Juice in Sheep

1972 ◽  
Vol 25 (1) ◽  
pp. 155 ◽  
Author(s):  
I Caple ◽  
T Heath
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Portal Vein ◽  
Pancreatic Juice ◽  
The Common ◽  
Bicarbonate Output

Bile and pancreatic juice were collected from conscious, standing sheep with fistulae of the common bile duct, before and during infusions of secretin to the portal vein, and during infusion of acid to the duodenum. The output of volume and electrolytes, particularly bicarbonate, in bile and in pancreatic juice increased during infusion of secretin. However, the output of volume and of bicarbonate was three to five times higher in bile than in pancreatic juice. When acid was infused into the duodenum a similar result was obtained, and the increment in total bicarbonate output was similar to the amount of acid infused.

Gastrin is not a physiological regulator of pancreatic exocrine secretion in the dog

1987 ◽  
Vol 252 (1) ◽  
pp. G40-G44
Author(s):  
E. Kohler ◽  
C. Beglinger ◽  
V. Eysselein ◽  
U. Grotzinger ◽  
K. Gyr
Keyword(s):  
Pancreatic Secretion ◽  
Pancreatic Enzyme ◽  
Exocrine Secretion ◽  
Exocrine Pancreatic Secretion ◽  
Response Curves ◽  
Blood Concentrations ◽  
Pancreatic Enzyme Secretion ◽  
Pancreatic Exocrine ◽  
Bicarbonate Output

The role of gastrin as a regulator of exocrine pancreatic secretion has not been proven adequately. In the present study we therefore compared the relative molar potencies of sulfated and unsulfated gastrin 17 with structurally related CCK peptides (synthetic CCK-8 and natural porcine CCK-33) in stimulating exocrine pancreatic secretion in conscious dogs. Dose response curves were constructed for pancreatic and gastric acid secretion. Plasma gastrin levels after exogenous gastrin 17-I and -II were compared with postprandial gastrin concentrations (meal: ground beef 20 g/kg body wt). The molar potency estimates calculated with synthetic CCK8 as standard (potency = 1.00) for pancreatic protein secretion were natural porcine 50% pure CCK-33 1.60, gastrin 17-I 0.12, and gastrin 17-II 0.16. All four peptides induced a dose-dependent increase in pancreatic bicarbonate output. However, the blood concentrations needed to stimulate pancreatic secretion were above the postprandial gastrin levels. Our data indicate that both gastrin 17 peptides are not physiological regulators of pancreatic enzyme secretion in dogs.

Stimulation of Pancreatic Enzyme Secretion by a Peptide Purified from Rat Bile-Pancreatic Juice

1986 ◽  
Vol 116 (8) ◽  
pp. 1540-1546 ◽  
Author(s):  
Shin-Ichi Fukuoka ◽  
Masahiro Tsujikawa ◽  
Tohru Fushiki ◽  
Kazuo Iwai
Keyword(s):  
Pancreatic Juice ◽  
Pancreatic Enzyme ◽  
Enzyme Secretion ◽  
Pancreatic Enzyme Secretion ◽  
Rat Bile ◽  
Stimulation Of
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