Effects and possible mechanism of tea tree oil against Botrytis cinerea and Penicillium expansum in vitro and in vivo test

2017 ◽  
Vol 63 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Yonghua Li ◽  
Xingfeng Shao ◽  
Jiayu Xu ◽  
Yingying Wei ◽  
Feng Xu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Botrytis Cinerea ◽  
Total Lipid ◽  
Penicillium Expansum ◽  
In Vitro Test ◽  
Respiratory Metabolism ◽  
Tea Tree Oil ◽  
Tea Tree

The purpose of this study was to investigate the antifungal activities and possible mechanisms of tea tree oil (TTO) against Botrytis cinerea and Penicillium expansum in vitro and in vivo. The results show that TTO exhibits dose-dependent antifungal activity against both pathogens, but P. expansum is less sensitive than B. cinerea to TTO not only in the in vitro test but also in artificially inoculated cherry fruits. TTO vapor treatment reduced the decay caused by these pathogens in inoculated cherry fruits, but the effect on P. expansum was less than that on B. cinerea. While the total lipid and ergosterol contents of the cell membrane are greater in P. expansum than in B. cinerea, TTO treatment lowers the total lipid content in the membranes of both species by well over 50%, and ergosterol content is reduced to a greater extent in B. cinerea than in P. expansum. In both pathogens, TTO alters mycelial morphology and cellular ultrastructure. Oxygen consumption measurements show that TTO inhibits respiratory metabolism via the tricarboxylic acid cycle pathway in both pathogens, though more severely in B. cinerea than in P. expansum. The relatively decreased sensitivity of P. expansum to TTO may be due to the fact that TTO causes less disruption of the cell membrane in this organism, and higher inhibition the respiratory metabolism to the extent observed in B. cinerea.

scholarly journals Emprego do óleo de Melaleuca alternifolia Cheel (Myrtaceae) na odontologia: perspectivas quanto à utilização como antimicrobiano alternativo às doenças infecciosas de origem bucal

2011 ◽  
Vol 13 (4) ◽  
pp. 492-499 ◽  
Author(s):  
A.C.M. Oliveira ◽  
A. Fontana ◽  
T.C. Negrini ◽  
M.N.M. Nogueira ◽  
T.B.L. Bedran ◽  
...  
Keyword(s):  
Tea Tree Oil ◽  
Melaleuca Alternifolia ◽  
Tea Tree

O interesse por medicamentos alternativos, principalmente daqueles provenientes de extratos naturais, tem aumentado nas últimas décadas. A Melaleuca alternifolia é um arbusto pertencente ao gênero Melaleuca, popularmente conhecida como "árvore de chá", cujo principal produto é o óleo essencial (TTO - tea tree oil), de grande importância medicinal por possuir comprovada ação bactericida e antifúngica contra diversos patógenos humanos. Em virtude da atividade terapêutica em diversas especialidades médicas, o TTO passou a ser empregado na área odontológica. Esta revisão de literatura foi realizada com o objetivo de discutir os ensaios já realizados com o TTO contra microrganismos relacionados à doença cárie, doença periodontal e problemas pulpares. O óleo de Melaleuca tem demonstrado boa ação antibacteriana in vitro contra microrganismos bucais, porém, pesquisas envolvendo o estudo do mecanismo de ação sobre as células microbianas ou estudos in vivo ainda são escassos e precisam ser realizados, já que esse produto pode ser útil na odontologia, seja na manutenção química da higiene ou prevenção de doenças bucais.

HERBAL CREAMS OF REISHI EXTRACT AND TEA TREE OIL FOR HIRSUTISM–AN IN VIVO STUDY

2020 ◽  
pp. 106-110
Author(s):  
SANGEETA CHOUDHURY ◽  
BLR MADHAVI
Keyword(s):  
Topical Application ◽  
Hair Growth ◽  
Ethanolic Extract ◽  
Hair Follicles ◽  
In Vivo Studies ◽  
Control Group ◽  
Tea Tree Oil ◽  
Tea Tree

Objective: The aim of this work to formulate, evaluate and compare the effectiveness of herbal creams containing extract of reishi and tea tree oil for treating hirsutism. Methods: Herbal ingredients were authenticated. Cream base was initially formulated. Three formulations of herbal cream were prepared. Reishi ethanolic extract, tea tree oil, and combination of tea tree oil and reishi extract were added to the cream base and formulated cream were named as RHC, THC and RTC respectively. In vitro evaluations on herbal creams were done for the physicochemical characteristics. In vivo studies were carried out on female Swiss Albino mice for the activity against hair growth by topical application of cream to shaved skin. The histological and morphometric evaluation was carried out. Skin irritancy study was conducted. Results: The herbal creams showed desirable physicochemical properties like pH, viscosity and spreadability. Statistical analysis for the length of hair was performed by using one way ANOVA followed by DUNNET’S post hoc test where THC and RTC were found to be significant whereas RHC showed no significant reduction of hair growth compared to control. RTC showed a significant effect at p<0.05 and hair growth reduction was significant for THC at p<0.001 compared to the control group. RTC and THC showed mild to moderate reduction in the size of the hair follicles with a reduction of sebaceous gland size in the histological analysis. Conclusion: Topical application of herbal creams to mice showed that hair growth was fastest in group RHC and was slowest in group THC and intermediate with RTC. It can be concluded that these herbal actives can be used as an effective treatment against hirsutism. Within the study period, tea tree oil was found to be more effective than reishi extract and the combination product. Further formulation studies and in vivo studies need to be carried out on reishi to assess its effectiveness against hirsutism.

scholarly journals In vitro and in vivo toxicity of several fungicides and Timorex gold biofungicide to Pythuim aphanidermatum

2013 ◽  
Vol 28 (2) ◽  
pp. 117-123 ◽  
Author(s):  
Milica Mihajlovic ◽  
Emil Rekanovic ◽  
Jovana Hrustic ◽  
Brankica Tanovic ◽  
Ivana Potocnik ◽  
...  
Keyword(s):  
Untreated Control ◽  
Control Plot ◽  
Tea Tree Oil ◽  
Greenhouse Soil ◽  
In Vivo Toxicity ◽  
Tea Tree ◽  
Better Than

A survey of in vitro and in vivo sensitivity of Pythuim aphanidermatum to several commercial fungicides and a biofungicide was undertaken. An isolate of P. aphanidermatum pathogenic to pepper was collected from a naturally infested greenhouse soil from Smederevska Palanka, Serbia. The P. aphanidermatum isolate was sensitive to all tested products. The obtained EC50 values were as follows: 10.21 mg/l for propamocarb-hydrochloride, 302.65 mg/l for fosetyl-Al, 11.18 mg/l for mancozeb, 1.27 mg/l for mefenoxam, 0.05 mg/l for azoxystrobin, and 175.33 mg/l for tea tree oil. Under greenhouse conditions, fosetyl-Al was the most efficient fungicide among the tested substances (97.5%). The biofungicide tea tree oil (Timorex Gold) (35.0%) exhibited the lowest efficacy among the tested materials, but it was still significantly better than the untreated control plot. The efficacies of propamocarb-hydrochloride (Previcur 607 SL), mancozeb (Mankogal 80 WP), azoxystrobin (Quadris) and mefenoxam (Ridomil gold 480 SL), were 72.5%, 77.5%, 57.5% and 75.0%, respectively.

Trypanocidal action of tea tree oil (Melaleuca alternifolia) against Trypanosoma evansi in vitro and in vivo used mice as experimental model

2014 ◽  
Vol 141 ◽  
pp. 21-27 ◽  
Author(s):  
Matheus D. Baldissera ◽  
Aleksandro S. Da Silva ◽  
Camila B. Oliveira ◽  
Roberto C.V. Santos ◽  
Rodrigo A. Vaucher ◽  
...  
Keyword(s):  
Experimental Model ◽  
Trypanosoma Evansi ◽  
Tea Tree Oil ◽  
Melaleuca Alternifolia ◽  
Tea Tree

scholarly journals Crotamiton-loaded tea tree oil containing phospholipid-based microemulsion hydrogel for scabies treatment: in vitro, in vivo evaluation, and dermatokinetic studies

Drug Delivery ◽  
2021 ◽  
Vol 28 (1) ◽  
pp. 1972-1981
Author(s):  
Lihua Chen ◽  
Majed Alrobaian ◽  
Obaid Afzal ◽  
Imran Kazmi ◽  
Sunil K. Panda ◽  
...  
Keyword(s):  
Tea Tree Oil ◽  
In Vivo Evaluation ◽  
Tea Tree

Efficacy of Essential Oil and Hydrosol Extract of Marrubium vulgare on Fungi Responsible for Apples Rot

2020 ◽  
Vol 18 (3) ◽  
pp. 285-293
Author(s):  
Amina Tabet Zatla ◽  
Imane Mami ◽  
Mohammed El Amine Dib ◽  
Mohammed El Amine Sifi
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Botrytis Cinerea ◽  
Alternaria Alternata ◽  
Penicillium Expansum ◽  
Caryophyllene Oxide ◽  
Marrubium Vulgare ◽  
In Vitro Antifungal Activity

Background: The microorganisms such as Penicillium expansum and Botrytis cinerea are wellknown pathogens in apples during postharvest. So, to protect apples from these pathogens, chemical control methods were exercised. Introduction: The main objective of this work was to study the chemical composition and the in-vitro and in-vivo antifungal properties of essential oil and hydrosol extract of Marrubium vulgare. Methods: In this work, the air-dried aerial parts of Marrubium vulgare were hydrodistilled in a Clevengertype apparatus. The essential oil and hydrosol extract isolated were analyzed using Gas Chromatography (GC) and Mass Spectrometry (GC/MS). The in-vitro antifungal activity of the both extracts was investigated against Botrytis cinerea, Penicillium expansum and Alternaria alternata fungi using radial growth technique. The effect of the essential oil and hydrosol extract on disease development of apple caused by Penicillium expansum in the in-vivo conditions was assessed. Results: The essential oil of Marrubium vulgare was characterized principally by E-β-caryophyllene (23.5%), E-β-farnesene (21%), α-humulene (14.8%), β-bisabolene (11.1%), caryophyllene oxide (6.8%) and phytol (3.1%). While, the methyl-eugenol (65.5%), α-Bisabolol (12.5%), linalool (6.5%) and caryophyllene oxide (6.2%) were the major compounds of hydrosol extract. The result of in-vitro antifungal activity of hydrosol extract showed an interesting antifungal inhibition against Botrytis cinerea, Penicillium expansum and Alternaria alternata with percentage inhibition ranging from 77% to 89% at low concentration of 0.15 mL/L. The essential oil was found to inhibit the growth of Penicillium expansum in a dose-dependent manner, with a percentage inhibition of 100% at 30 mL/L. Furthermore, essential oil and hydrosol extract have demonstrated promising in-vivo antifungal activity to control infection of apples by Penicillium expansum up to 25th day of storage, compared with the control. Conclusion: The preventive and protective effects of essential oil and hydrosol extract could be exploited as an ideal alternative to synthetic fungicides for using the protection of stored apples from fungal phytopathogens.

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