Production ofBacillus amyloliquefaciensOG and its metabolites in renewable media: valorisation for biodiesel production andp-xylene decontamination

2017 ◽  
Vol 63 (1) ◽  
pp. 46-60 ◽  
Author(s):  
Augusto Etchegaray ◽  
François Coutte ◽  
Gabrielle Chataigné ◽  
Max Béchet ◽  
Ramon H.Z. dos Santos ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Large Scale ◽  
Production Costs ◽  
Biodiesel Production ◽  
Scale Production ◽  
Specific Production ◽  
Primary And Secondary Metabolites ◽  
Large Scale Production ◽  
Promote Plant Growth ◽  
Acetoin Production

Biosurfactants are important in many areas; however, costs impede large-scale production. This work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of Bacillus amyloliquefaciens. Initially, Bacillus sp. strain 0G was renamed B. amyloliquefaciens subsp. plantarum (syn. Bacillus velezensis) after analysis of the gyrA and gyrB DNA sequences. Growth in modified Landy’s medium produced 3 main recoverable metabolites: surfactin, fengycin, and acetoin, which promote plant growth. Cultivation was studied in the presence of renewable carbon (as glycerol) and nitrogen (as arginine) sources. While diverse kinetics of acetoin production were observed in different media, similar yields (6–8 g·L–1) were obtained after 72 h of growth. Glycerol increased surfactin-specific production, while arginine increased the yields of surfactin and fengycin and increased biomass significantly. The specific production of fengycin increased ∼10 times, possibly due to a connecting pathway involving arginine and ornithine. Adding value to crude extracts and biomass, both were shown to be useful, respectively, for the removal of p-xylene from contaminated water and for biodiesel production, yielding ∼70 mg·g–1cells and glycerol, which could be recycled in novel media. This is the first study considering circular bioeconomy to lower the production costs of biosurfactants by valorisation of both microbial cells and their primary and secondary metabolites.

scholarly journals Recent Advances in Glycerol Catalytic Valorization: A Review

Catalysts ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1279
Author(s):  
Manuel Checa ◽  
Sergio Nogales-Delgado ◽  
Vicente Montes ◽  
José María Encinar
Keyword(s):  
Activated Carbon ◽  
Carboxylic Acids ◽  
Large Scale ◽  
Biodiesel Production ◽  
Added Value ◽  
Scale Production ◽  
Recent Advances ◽  
Large Scale Production ◽  
Wide Range ◽  
Technological Limitations

Once a biorefinery is ready to operate, the main processed materials need to be completely evaluated in terms of many different factors, including disposal regulations, technological limitations of installation, the market, and other societal considerations. In biorefinery, glycerol is the main by-product, representing around 10% of biodiesel production. In the last few decades, the large-scale production of biodiesel and glycerol has promoted research on a wide range of strategies in an attempt to valorize this by-product, with its transformation into added value chemicals being the strategy that exhibits the most promising route. Among them, C3 compounds obtained from routes such as hydrogenation, oxidation, esterification, etc. represent an alternative to petroleum-based routes for chemicals such as acrolein, propanediols, or carboxylic acids of interest for the polymer industry. Another widely studied and developed strategy includes processes such as reforming or pyrolysis for energy, clean fuels, and materials such as activated carbon. This review covers recent advances in catalysts used in the most promising strategies considering both chemicals and energy or fuel obtention. Due to the large variety in biorefinery industries, several potential emergent valorization routes are briefly summarized.

scholarly journals Nanomedicine for Gene Delivery for the Treatment of Cardiovascular Diseases

2019 ◽  
Vol 19 (1) ◽  
pp. 20-30 ◽  
Author(s):  
Cen Yan ◽  
Xiao-Jiang Quan ◽  
Ying-Mei Feng
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Dna Sequences ◽  
Large Scale ◽  
Heart Function ◽  
Current Status ◽  
Future Perspective ◽  
Scale Production ◽  
Large Scale Production ◽  
Target Molecules

Background: Myocardial infarction (MI) is the most severe ischemic heart disease and directly leads to heart failure till death. Target molecules have been identified in the event of MI including increasing angiogenesis, promoting cardiomyocyte survival, improving heart function and restraining inflammation and myocyte activation and subsequent fibrosis. All of which are substantial in cardiomyocyte protection and preservation of cardiac function. Methodology: To modulate target molecule expression, virus and non-virus-mediated gene transfer have been investigated. Despite successful in animal models of MI, virus-mediated gene transfer is hampered by poor targeting efficiency, low packaging capacity for large DNA sequences, immunogenicity induced by virus and random integration into the human genome. Discussion: Nanoparticles could be synthesized and equipped on purpose for large-scale production. They are relatively small in size and do not incorporate into the genome. They could carry DNA and drug within the same transfer. All of these properties make them an alternative strategy for gene transfer. In the review, we first introduce the pathological progression of MI. After concise discussion on the current status of virus-mediated gene therapy in treating MI, we overview the history and development of nanoparticle-based gene delivery system. We point out the limitations and future perspective in the field of nanoparticle vehicle. Conclusion: Ultimately, we hope that this review could help to better understand how far we are with nanoparticle-facilitated gene transfer strategy and what obstacles we need to solve for utilization of nanomedicine in the treatment of MI.

scholarly journals Toward Improvement of Erythromycin A Production in an Industrial Saccharopolyspora erythraea Strain via Facilitation of Genetic Manipulation with an ArtificialattBSite for Specific Recombination

2011 ◽  
Vol 77 (21) ◽  
pp. 7508-7516 ◽  
Author(s):  
Jiequn Wu ◽  
Qinglin Zhang ◽  
Wei Deng ◽  
Jiangchao Qian ◽  
Siliang Zhang ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Large Scale ◽  
Genetic Manipulation ◽  
Saccharopolyspora Erythraea ◽  
Scale Production ◽  
Content Type ◽  
Large Scale Production ◽  
Endogenous Genes ◽  
Erythromycin A ◽  
Adenosylmethionine Synthetase

ABSTRACTLarge-scale production of erythromycin A (Er-A) relies on the organismSaccharopolyspora erythraea, in which lack of a typicalattBsite largely impedes the application of phage ΦC31 integrase-mediated recombination into site-specific engineering. We herein report construction of an artificialattBsite in an industrialS. erythraeastrain, HL3168 E3, in an effort to break the bottleneck previously encountered during genetic manipulation mainly from homologous or unpredictable nonspecific integration. Replacement of a cryptic gene,nrps1-1, with a cassette containing eightattBDNA sequences did not affect the high Er-producing ability, setting the stage for precisely engineering the industrial Er-producing strain for foreign DNA introduction with a reliable conjugation frequency. Transfer of either exogenous or endogenous genes of importance to Er-A biosynthesis, including theS-adenosylmethionine synthetase gene for positive regulation,vhbfor increasing the oxygen supply, and two tailoring genes,eryKanderyG, for optimizing the biotransformation at the late stage, was achieved by taking advantage of this facility, allowing systematic improvement of Er-A production as well as elimination of the by-products Er-B and Er-C in fermentation. The strategy developed here can generally be applicable to other strains that lack theattBsite.

scholarly journals Polyhyrdoxybutyrate production by Bacillus marcorestinctum using a Cheaper substrate and its electrospinned blends with polymer

2019 ◽  
Author(s):  
Swetha Narayankumar ◽  
Neethu K. Shaji ◽  
Veena gayathri Krishnaswamy
Keyword(s):  
Carbon Source ◽  
Large Scale ◽  
C:N Ratio ◽  
Scale Up ◽  
Production Costs ◽  
Scale Production ◽  
Transmission Electron ◽  
Large Scale Production ◽  
Dry Cell Weight ◽  
Alternative Carbon Source

ABSTRACTPoly(hydroxybutyric acid) (PHB) and other biodegradable polyesters are promising candidates for the development of environment-friendly and completely biodegradable plastics. One of the major drawbacks in the production of PHB is production costs, since it requires large amount of carbon source. This calls for cheaper substrates that can be used as an alternative carbon source such as agro-industrial residues. In this study, cane molasses was used as an additional carbon source at 2% concentration along with glucose for large scale production of PHB. Ammonium nitrate was used as the nitrogen source and the C:N ratio was maintained at 1:15. The maximum production of PHB was obtained at 24hours of growth which was found to be 0.5g/L and had a dry cell weight of 3.7g/ L.The PHB produced was further analysed by GC-MS Analysis and Transmission Electron Microscopy (TEM).The obtained PHB from scale-up studies were further electrospinned using different blends of polymers.

scholarly journals Production of L-theanine by Escherichia coli in the absence of supplemental ethylamine

2021 ◽  
Author(s):  
Ryota Hagihara ◽  
Shoto Ohno ◽  
Mikiro Hayashi ◽  
Kazuhiko Tabata ◽  
Hirofumi Endo
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Syringae ◽  
Industrial Production ◽  
Large Scale ◽  
Food Additives ◽  
Production Capacity ◽  
Production Costs ◽  
Scale Production ◽  
Large Scale Production ◽  
Tea Plants

l-Theanine is a nonproteinogenic amino acid present almost exclusively in tea plants and is beneficial for human health. For industrial production, l-theanine is enzymatically or chemically synthesized from glutamine/glutamate (or a glutamine/glutamate derivative) and ethylamine. Ethylamine is extremely flammable and toxic, which complicates and increases the cost of operational procedures. To solve these problems, we developed an artificial biosynthetic pathway to produce l-theanine in the absence of supplemental ethylamine. For this purpose, we identified and selected the novel transaminase AAN70747 from Pseudomonas putida KT2440, which catalyzes the transamination of acetaldehyde to produce ethylamine, as well as γ-glutamylmethylamide synthetase AAY37316 from Pseudomonas syringae pv. syringae B728a, which catalyzes the condensation of l-glutamate and ethylamine to produce l-theanine. Expressing these genes in Escherichia coli W3110S3GK and enhancing the production capacity of acetaldehyde and l-alanine achieved successful production of l-theanine without ethylamine supplementation. Furthermore, the deletion of ggt, which encodes γ-glutamyltranspeptidase (EC 2.3.2.2), achieved large-scale production of l-theanine by attenuating its decomposition. We show that an alanine decarboxylase-utilizing pathway represents a promising route for the fermentative production of l-theanine. To our knowledge, this is the first report of efficient methods to produce l-theanine in the absence of supplemental ethylamine. IMPORTANCE l-Theanine is widely used in food additives and dietary supplements. Industrial production of l-theanine uses the toxic and highly flammable precursor ethylamine, raising production costs. Here we used Escherichia coli to engineer two biosynthetic pathways that produce l-theanine from glucose and ammonia in the absence of supplemental ethylamine. This study establishes a foundation for safely and economically producing l-theanine.

Gene therapy using MnO2 nanoparticles

2021 ◽  
Author(s):  
Moataz Dowaidar
Keyword(s):  
Clinical Studies ◽  
Large Scale ◽  
Tumor Development ◽  
Specific Area ◽  
Production Costs ◽  
Scale Production ◽  
Large Scale Production ◽  
Mno2 Nanoparticles ◽  
Nanobuilding Blocks ◽  
Tumor Accumulation

Due to its unique tumor microenvironment (TME)-sensitive breakdown, O2 generation, and GSH depletion, the MnO2-based nanostructure has been widely used as an efficiently enhanced therapeutic medication to inhibit tumor development or even eradicate tumor tissue. From controllable manufacturing of MnO2 nanobuilding blocks to targeting tumor accumulation, working mechanisms to enhance therapeutic efficacy, and necessary considerations for building all-in-one nanoplatforms, this review systematically summarized strategies for designing all-in-one MnO2-based nanoplatforms for accurate and synergistic therapy. MnO2-based nanostructures are a promising functional nanocarrier to construct all-in-one nanoplatforms due to their controlled size and shape, high specific area and low production costs. Large-scale production and monodispersing in various cultural media are the current primary barriers to future biomedical usage. This review addresses techniques to create MnO2 nanoparticles for accurate, synergistic treatment. Clinical studies may leverage the all-in-one nanoplatform to boost treatment performance. Some significant problems need to be resolved before it can be utilized for clinical studies. Material manufacturing, reproducible preparation, and maintaining blood circulation function are still a challenge. Following intravenous injection, accurate monitoring of material dispersion in live beings and assessing the biological long-term consequences of materials on organisms are major issues.

scholarly journals HUMAN RESOURCES TRAINING IN BIOTECHNOLOGY: MICROALGAE FOR BIOFUEL AND WASTEWATER TREATMENT.

2019 ◽  
Vol 18 (1) ◽  
pp. 5
Author(s):  
Iracema De Oliveira Moraes ◽  
Regina De Oliveira Moraes Arruda ◽  
Rodrigo De Oliveira Moraes ◽  
Maria Josiane Conti Moraes
Keyword(s):  
Wastewater Treatment ◽  
Human Resources ◽  
Large Scale ◽  
Biodiesel Production ◽  
Scale Production ◽  
Third Generation ◽  
Competitive Price ◽  
Large Scale Production ◽  
The Subject ◽  
Very High

The most promising and innovative alternative to biodiesel production is presented by the algae (micro and macroalgae), which have been classified by scientists as a source of third generation biofuels. The large-scale production of biodiesel from microalgae and macroalgae bioethanol production will happen much faster than you think. It is believed that its full commercialization is possible within a few years, and with a competitive price compared to diesel produced from petroleum, the same occurring for bioethanol. The use of seaweed as a feedstock for the production of biofuels has been seen as a less environmentally impactful as the biomass produced on the continent and its potential is very high. Several groups in Latin America (Brazil, of course) are studying micro and macroalgae not only for biofuels production (biodiesel, bioethanol, biohydrogen) but also to do wastewater treatment. This paper will discuss the presentations done in four annual courses (2010, 2011, 2012, 2013) promoted by the Biotechnology Brazilian Argentine Center, BBAC, and offered to fellowships (sixteen per year), from Brazil, Argentina, Colombia, Paraguay and Uruguay. Arthrospira (Spirulina) platensis was chosen as a cyanobacteria model in the courses, due to its feasibility of cultivation, many publications about the subject and the existence of strains isolated in Brazil.

scholarly journals An Innovative Vegetative Propagation System for Large-scale Production of Globe Artichoke Transplants. Part II. Propagation System Validation

2005 ◽  
Vol 15 (4) ◽  
pp. 817-819 ◽  
Author(s):  
Mariateresa Cardarelli ◽  
Youssef Rouphael ◽  
Francesco Saccardo ◽  
Giuseppe Colla
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Central Italy ◽  
Production Costs ◽  
Production Cycle ◽  
Scale Production ◽  
Globe Artichoke ◽  
Large Scale Production ◽  
Propagation Technique

Research was conducted at the University of Tuscia (central Italy) to validate the propagation system for globe artichoke (Cynara cardunculus var. scolymus) described in a previous paper for a 1-year production cycle. The resulting globe artichoke plants were used in a 2-year field trial to investigate the field response of plantlets obtained with our propagation technique in comparison with plantlets produced by in vitro propagation and by offshoots harvested in commercial fields. The total number of artichoke plantlets obtained with our propagation system was 62.7 plantlets/m2 per year. In the first year, the globe artichoke production (bud number and fresh bud weight) was higher in plants obtained with our propagation system and by micropropagation than in those obtained from offshoots harvested in commercial fields. The production cost of plantlets obtained with our propagation technique was 52% lower than those of the micropropagated plantlets. This could lead to a significant reduction of production costs for artichoke growers, while preserving the advantages of in vitro propagation (disease-free plants and plant uniformity).

scholarly journals Characterization of MOCVD-Prepared CIS Solar Cells

Energies ◽  
2021 ◽  
Vol 14 (22) ◽  
pp. 7721
Author(s):  
Seung Hoon Lee ◽  
Gyu Hyun Lee ◽  
Hae-Seok Lee ◽  
Donghwan Kim ◽  
Yoonmook Kang
Keyword(s):  
Solar Cells ◽  
Large Scale ◽  
Chemical Vapor ◽  
Energy Conversion Efficiency ◽  
Production Costs ◽  
Organic Chemical ◽  
Scale Production ◽  
Photovoltaic Devices ◽  
Large Scale Production ◽  
Cigs Solar Cells

Chalcopyrite Cu(In,Ga)Se2 (CIGS) solar cells prepared via metal-organic chemical vapor deposition (MOCVD) are one of the candidates for highly advanced photovoltaic devices. This is because of their effectiveness and potential for reducing production costs through large-scale production. However, research on MOCVD-prepared solar cells is progressing slower than that on other types of solar cells, primarily because the preparation of CuInSe2 (CIS)-based films via MOCVD is relatively more sophisticated. In this study, we analyzed CIS solar cells prepared via three-stage MOCVD and processed with relatively simple precursors and techniques. We achieved an energy-conversion efficiency of 7.39% without applying a buffer layer. Instead, we applied a Cu-deficient layer to create a buried pn junction. Ultimately, we demonstrated that the fabrication of fully-MOCVD-processed CIS photovoltaic devices is feasible.

scholarly journals The Perspective of Large-Scale Production of Algae Biodiesel

2020 ◽  
Vol 10 (22) ◽  
pp. 8181
Author(s):  
Mladen Bošnjaković ◽  
Nazaruddin Sinaga
Keyword(s):  
Large Scale ◽  
Diesel Oil ◽  
Biodiesel Production ◽  
Scale Production ◽  
Algae Biomass ◽  
Electric Cars ◽  
Algae Biodiesel ◽  
Large Scale Production ◽  
Algal Biodiesel ◽  
The Impact

We have had high expectations for using algae biodiesel for many years, but the quantities of biodiesel currently produced from algae are tiny compared to the quantities of conventional diesel oil. Furthermore, no comprehensive analysis of the impact of all factors on the market production of algal biodiesel has been made so far. This paper aims to analyze the strengths, weaknesses, opportunities, and threats associated with algal biodiesel, to evaluate its production prospects for the biofuels market. The results of the analysis show that it is possible to increase the efficiency of algae biomass production further. However, because the production of this biodiesel is an energy-intensive process, the price of biodiesel is high. Opportunities for more economical production of algal biodiesel are seen in integration with other processes, such as wastewater treatment, but this does not ensure large-scale production. The impact of state policies and laws is significant in the future of algal biodiesel production. With increasingly stringent environmental requirements, electric cars are a significant threat to biodiesel production. By considering all the influencing factors, it is not expected that algal biodiesel will gain an essential place in the fuel market.

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