In vitro probiotic profiling of novel Enterococcus faecium and Leuconostoc mesenteroides from Tunisian freshwater fishes

2016 ◽  
Vol 62 (1) ◽  
pp. 60-71 ◽  
Author(s):  
Rim El-Jeni ◽  
Monia El Bour ◽  
Pilar Calo-Mata ◽  
Karola Böhme ◽  
Inmaculada C. Fernández-No ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Potential Application ◽  
Leuconostoc Mesenteroides ◽  
Antimicrobial Agents ◽  
Food Poisoning ◽  
Heat Processing ◽  
Probiotic Properties ◽  
Processed Foods ◽  
Adhesion Ability

Novel lactic acid bacteria isolated from different organs of freshwater fish were examined for their potential application as probiotics in raw and processed foods. Four isolates of Enterococcus faecium and Leuconostoc mesenteroides were identified at the molecular level by 16S rRNA sequencing and random amplification of polymorphic DNA – polymerase chain reaction, and their antimicrobial activity against a panel of pathogens and food-poisoning bacteria was investigated. The whole bacteriocins of the 4 isolates were characterized by enterobacterial repetitive intergenic consensus sequences in PCR. The isolates exhibited high inhibitory activities against food-borne pathogens and spoilage microbial species and have significant probiotic profiles, since they survived at pH 3.0 and in the presence of bile salts, pancreatin, and pepsin, without any detectable hemolytic activity. Further, moderate heat resistance, adhesion ability to steel surfaces, and sensitivity to clinically relevant antimicrobial agents were revealed for all the isolates. These results highlight the specific probiotic properties of the strains and give evidence for potential application in minimally processed foods subjected to moderate heat processing.

scholarly journals Technological Aptitude and Applications ofLeuconostoc mesenteroidesBioactive Strains Isolated from Algerian Raw Camel Milk

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Zineb Benmechernene ◽  
Hanane Fatma Chentouf ◽  
Bellil Yahia ◽  
Ghazi Fatima ◽  
Marcos Quintela-Baluja ◽  
...  
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Antimicrobial Agents ◽  
High Capacity ◽  
Mixed Cultures ◽  
Camel Milk ◽  
High Survival ◽  
Probiotic Properties ◽  
Specific Pcr ◽  
Listeria Ivanovii

Two strains (B7 and Z8) of theLeuconostoc mesenteroidessubspeciesmesenteroidesthat were isolated from Algerian camel milk from an initial pool of 13 strains and demonstrated a high ability to inhibit the growth ofListeriaspp. were selected and characterised at the phenotypic and genotypic levels. Probiotic profiling and inhibition spectra against food borne pathogens in mixed cultures were also investigated. The bacteriocin produced byL. mesenteroidesstrain B7 was identified as leucocin B by specific PCR.In vitrostudies demonstrated that bothLeuconostoc mesenteroidesstrains exhibited a marked probiotic profile, showing high survival at low pH (2-3 and 4) in the presence of 0.5%, 1%, and 2% of bile salts and at pH 3 in the presence of 3 mg/mL pepsin. Susceptibility testing against antimicrobial agents was also performed for both strains. When tested in a mixed culture withListeria innocua,Listeria ivanovii, orStaphylococcus aureus, strain B7 reduced the numbers of these species by 1.87, 1.78, and 1.38 log units, respectively. Consequently, these two strains were found to possess good probiotic propertiesin vitroand a high capacity forListeriaspp. inhibition in mixed cultures. Therefore, these strains have a favourable technological aptitude and a potential application as novel probiotic starters.

Some probiotic properties of chicken lactobacilli

1999 ◽  
Vol 45 (12) ◽  
pp. 981-987 ◽  
Author(s):  
Carlos Gusils ◽  
Silvia N González ◽  
G Oliver
Keyword(s):  
Probiotic Properties ◽  
Epithelial Surface ◽  
Adhesion Ability ◽  
Bivalent Cations ◽  
Calcium Requirement ◽  
Salmonella Pullorum ◽  
Vitro Model ◽  
Lactobacillus Animalis

The beneficial effect of lactobacilli has been attributed to their ability to colonize human and animal gastrointestinal tracts. In this work, adhesion assays with three lactobacillus strains and intestinal fragments obtained from chickens were assessed. Lactobacillus animalis and L. fermentum were able to adhere to three kinds of epithelial cells (crop, small and large intestines) with predominance to small intestine. Among the strains considered, L. fermentum subsp. cellobiosus showed the lowest and L. animalis the highest adhesion ability. Scanning electron microphotographs showing L. animalis and L. fermentum adhering to intestinal cells were obtained. The characterization of L. animalis adhesion indicated that lectin-like structure of this strain has glucose/mannose as specific sugars of binding. However, a calcium requirement was not observed. The adhesion of L. fermentum was reduced by addition of sialic acid or mannose (P < 0.01). These carbohydrates can be involved in the interaction between adhesin and epithelial surface. In this case, the dependence on bivalent cations was demonstrated. Lactobacillus fermentum was effective in reducing the attachment of Salmonella pullorum by 77%, while L. animalis was able to inhibit (90%, 88%, and 78%) the adhesion of S. pullorum, S. enteritidis, and S. gallinarum to host-specific epithelial fragments respectively. Our results from this in vitro model suggest that these lactobacilli are able to block the binding sites for Salmonella adhesion.Key words: lactobacilli, lectin-like structures, poultry adhesion, probiotic properties.

scholarly journals Inadequate Cerebrospinal Fluid Concentrations of Available Salvage Agents Further Impedes the Optimal Treatment of Multidrug-Resistant Enterococcus faecium Meningitis and Bacteremia

2021 ◽  
Vol 13 (3) ◽  
pp. 843-854
Author(s):  
Eric Wenzler ◽  
Alina Adeel ◽  
Tiffany Wu ◽  
Michele Jurkovic ◽  
Jeremy Walder ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Enterococcus Faecium ◽  
Antimicrobial Agents ◽  
Treatment Options ◽  
Complex Case ◽  
Combination Regimen ◽  
In Vitro Susceptibility ◽  
Hematopoietic Stem ◽  
Time Kill

Background: Vancomycin-resistant Enterococcus faecium (VRE) in particular has evolved as an important cause of hospital acquired infection, especially in immunocompromised hosts. Methods: We present a complex case of a patient with relapsed acute myeloid leukemia who underwent allogenic hematopoietic stem cell transplantation complicated by persistent VRE bacteremia and meningitis. To optimize therapy, various blood and cerebrospinal fluid (CSF) samples were sent to a research laboratory for extensive susceptibility testing, pharmacokinetic analyses, and time-kill experiments. Results: In vitro testing revealed resistance to all first-line treatment options and CSF sampling demonstrated sub-optimal central nervous system concentrations achieved by each antimicrobial agent administered in relation to their respective MIC value. Time-kill analyses at observed CSF concentrations confirmed the lack of bactericidal activity despite use of a four-drug combination regimen. Conclusions: This work is the first to report CSF concentrations of oritavancin and tedizolid in humans and adds to the limited data regarding in vitro susceptibility of new antimicrobial agents such as eravacycline, omadacycline, and lefamulin against VRE. Our study provides new insights into various aspects of treatment of extensively drug-resistant Enterococcus faecium meningitis and bacteremia and supports the continued pursuit of precision medicine for these challenging cases.

scholarly journals  Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro

2012 ◽  
Vol 57 (No. 11) ◽  
pp. 529-539 ◽  
Author(s):  
K. Saelim ◽  
N. Sohsomboon ◽  
S. Kaewsuwan ◽  
S. Maneerat
Keyword(s):  
Enterococcus Faecium ◽  
Probiotic Strain ◽  
Antagonistic Effect ◽  
Penicillin G ◽  
Probiotic Properties ◽  
Antibiotic Resistant ◽  
Good Tolerance ◽  
Probiotic Potential ◽  
Enterocin A

A bacteriocin-like substance (BLS) producing Enterococcus faecium CE5-1 was isolated from the gastrointestinal tract (GIT) of Thai indigenous chickens. Investigations of its probiotic potential were carried out. The competition between the BLS probiotic strain and antibiotic-resistant enterococci was also studied. Ent. faecium CE5-1 exhibited a good tolerance to pH 3.0 after 2 h and in 7% fresh chicken bile after 6 h, but the viability of Ent. faecium CE5-1 decreased by about 2&ndash;3 log CFU/ml after 2 h incubation in pH 2.5. It was susceptible to the antibiotics tested (tetracycline, erythromycin, penicillin G, and vancomycin). The maximum BLS production from Ent. faecium CE5-1 was observed at 15 h of cultivation. It showed activity against Listeria monocytogenes DMST17303, Pediococcus pentosaceus 3CE27, Lactobacillus sakei subsp. sakei JCM1157, and antibiotic-resistant enterococci. The detection by polymerase chain reaction (PCR) in the enterocin structural gene determined the presence of enterocin A gene in Ent. faecium CE5-1 only. Ent. faecium CE5-1 showed the highest inhibitory activity against two antibiotic-resistant Ent. faecalis VanB (from 6.68 to 4.29 log CFU/ml) and Ent. gallinarum VanC (from 6.76 to 4.31 log CFU/ml) after 12 h of co-cultivation. The results show the future possible use of Ent. faecium CE5-1 as a probiotic strain for livestock to control antibiotic-resistant enterococci. &nbsp;

scholarly journals Novel 2,4,6-Trimethylbenzenesulfonyl Hydrazones with Antibacterial Activity: Synthesis and In Vitro Study

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 2723
Author(s):  
Łukasz Popiołek ◽  
Sylwia Szeremeta ◽  
Anna Biernasiuk ◽  
Monika Wujec
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Potential Application ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Condensation Reaction ◽  
In Vitro Study ◽  
In Vitro Bioactivity ◽  
Bacterial Strains

This research describes the synthesis and in vitro antimicrobial activity study of a series of 2,4,6-trimethylbenzenesulfonyl hydrazones. Twenty-five hydrazones (2–26) were synthesized on the basis of condensation reaction. The in vitro bioactivity study confirmed the potential application of obtained derivatives as antimicrobial agents. Among the tested compounds, the highest activity was discovered for derivative 24, which possessed minimal inhibitory concentration (MIC) ranging from 7.81 to 15.62 µg/mL against Gram-positive reference bacterial strains. Synthesized benzenesulfonyl hydrazones can be applied as potential ligands for the synthesis of bioactive metal complexes.

scholarly journals In Vitro Probiotic Properties and DNA Protection Activity of Yeast and Lactic Acid Bacteria Isolated from A Honey-Based Kefir Beverage

Foods ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 485 ◽  
Author(s):  
Bruna de Oliveira Coelho ◽  
Fernanda Fiorda-Mello ◽  
Gilberto de Melo Pereira ◽  
Vanete Thomaz-Soccol ◽  
Sudip Rakshit ◽  
...  
Keyword(s):  
Oxidative Dna Damage ◽  
World Health ◽  
Probiotic Properties ◽  
Dna Protection ◽  
Protective Capacity ◽  
Adhesion Ability ◽  
Dpph Method ◽  
Microbial Strains ◽  
Health Organization

The probiotic characteristics of three acid-tolerant microbial strains, viz., Lactobacillus satsumensis LPBF1, Leuconostoc mesenteroides LPBF2 and Saccharomyes cerevisiae LPBF3, isolated from a honey-based kefir functional beverage, were studied following the requirements established by the Food and Agriculture Organization of the United Nation/World Health Organization (FAO/WHO), including host-associated stress resistance, epithelium adhesion ability, and antimicrobial activity. The three microbial strains tolerated different pH values (2.0, 3.0, 4.0 and 7.0) and bile salt concentrations (0.3% and 0.6%), and survive in the presence of simulated gastric juice, which are conditions imposed by the gastrointestinal tract. In addition, they showed high percentages of hydrophobicity, auto aggregation and anti-pathogenic against Escherichia coli and Staphylococcus aureus, with no hemolytic activity. The protective capacity of human DNA through microbial treatment was investigated by single-cell gel electrophoresis (SCGE) comet assay. The three selected strains showed DNA protection effect against damage caused by hydroxyl radical (H2O2). However, when the S. cerevisiae treatment was applied, the most effective DNA protection index was observed, which can be associated to its high production of extracellular antioxidants as reveled by the 2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH) method. These results indicated that the three selected microbial strains could be useful for preventing oxidative DNA damage and cellular oxidation in food products. As well-adapted microbial cells, the selected strains can be used for production of non-dairy functional beverages, especially for vegans and/or vegetarians and lactose intolerants.

scholarly journals MOLECULAR IDENTIFICATION OF PROBIOTIC YEAST STRAINS AND THEIR CHARACTERIZATION

2017 ◽  
Vol 10 (10) ◽  
pp. 339 ◽  
Author(s):  
Mangala Lakshmi Ragavan ◽  
Nilanjana Das
Keyword(s):  
Molecular Level ◽  
Kluyveromyces Lactis ◽  
In Vitro Assays ◽  
In Vitro Toxicity ◽  
Probiotic Properties ◽  
Lipomyces Starkeyi ◽  
Adhesion Ability ◽  
Yeast Strains ◽  
Probiotic Yeast

Objective The objective of the present study was to identify the potential yeast isolates at themolecular level and evaluate their probiotic characteristics.MethodsMolecular characterization was done for 5 potential probiotic yeast strains. In vitro assays have been conducted to evaluate the probiotic properties such as NaCl tolerance, autoaggregation and co-aggregation. Haemolyticactivity, urease activity and cytotoxicity tests were carried out for safety assay during the characterization of yeast strains.ResultsIn this study, the yeast strains viz. LM,MR,GOI,GII2and WI were identified at molecular level and named as Yarrowialipolytica VIT-MN01, Kluyveromyces lactis VIT-MN02, Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligera VIT-MN04, Brettanomyces custersianus VIT-MN05 respectively. Maximum autoaggregation and coaggregationwere noted in case of Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligeraVIT-MN04, and Brettanomyces custersianus VIT-MN05. In vitro toxicity assay was performed and all the yeast strains showednon-toxic nature.ConclusionFive yeast strains have been studied for their probiotic characteristics and identified at molecular level. Out offive yeast strains, three strains showed maximum adhesion ability,which is a prerequisite for colonization and protection of gastrointestinal tract. All the yeast strains are validated as a safe bioresources because of their non - hemolytic activities and non-production of urease. It can be concluded that the identified yeast strainscan serve as promising probiotics in various fields offood industry.

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