Molecular analysis of genes related to phenylpropanoid and ascorbate biosynthesis in salt and UV-B treated pak choi grown under LEDs

Botany ◽  
2019 ◽  
Vol 97 (9) ◽  
pp. 513-519 ◽  
Author(s):  
Young Beom Yun ◽  
Hee-Jeong Jung ◽  
Md Abdur Rahim ◽  
Jong-In Park ◽  
Yong In Kuk
Keyword(s):  
Light Emitting Diode ◽  
Phenylpropanoid Pathway ◽  
Light Conditions ◽  
Pak Choi ◽  
Light Emitting ◽  
Nutraceutical Compounds ◽  
Enhanced Production ◽  
Expression Of Genes ◽  
Significant Difference ◽  
Phenylpropanoid Biosynthesis

In this study, we measured the expression of genes related to the phenylpropanoid pathway and ascorbate oxidation by qRT-PCR to explore the mechanism of nutraceutical compounds enhancement in pak choi during stress treatments. The expression of most phenylpropanoid biosynthesis related genes (PAL, C4H, CHS, CHI, F3H1, DFR, and ANS1) was up-regulated in plants treated with NaCl, solar salt, and UV-B radiation, compared with the untreated controls under both white light and light emitting diode illumination. Moreover, CHS and ANS1 were expressed at levels 15 and 95 times higher than the untreated control. The expression of the ascorbate oxidation related gene tAPX was also up-regulated in the NaCl and UV-B treated samples compared with the control under both light conditions. By comparison, the expression of APX1 was down-regulated during the stress treatment under both light conditions. Total chlorophyll and total carotenoid contents were unaffected by the stress treatments, and there was no significant difference in the maximum quantum yield (Fv/Fm) between the stress treatments and the untreated controls. These results suggest that the expression of genes involved in phenylpropanoid biosynthesis and ascorbate oxidation correlates with the previously reported enhanced production of phenolic compounds in pak choi plants subjected to stress treatments.

scholarly journals Analysis of Energy and Cost Performance of Retrofitting Fluorescent Tubes with Compact Fluorescent and Light Emitting Diode Lamps

2020 ◽  
pp. 34-46 ◽  
Author(s):  
J. S. Adeleke ◽  
A. B. Wahab ◽  
E. A. Olanipekun
Keyword(s):  
Light Emitting Diode ◽  
Electricity Consumption ◽  
Poor Performance ◽  
Payback Period ◽  
Optimal Performance ◽  
Light Emitting ◽  
Tertiary Institutions ◽  
Fluorescent Lamps ◽  
Significant Difference ◽  
Compact Fluorescent Lamp

Over the years, visual comfort has been described as a major requirement needed to enhance optimal performance of occupants in any learning environment in tertiary institutions. This is reflected in the poor performance of the commonly used Fluorescent Lamps (FL) occasioned by its constant burn-off, high failure rate and reduced durability. Hence, there is a shift to the adoption of Compact Fluorescent Lamp (CFL) and Light Emitting Diode (LED). This study therefore examined the pattern of electricity consumption involving the adoption of FL, CFL and LED in the purposively selected lecture theatres of Obafemi Awolowo University, Ile-Ife, Nigeria. The study was carried out using energy monitor to obtain the consumption data of the lamps for periodic logging at intervals of 1 hr, 3 hrs, 6 hrs, 12 hrs, 1 day, 7 days, 1 month and 3 months respectively; and also to determine the payback period of each of the lamps. From the data collected, patterns of electricity used, electricity savings were calculated and compared to establish performance potentials of each of the lamps. The study found that there was a significant difference in the pattern of electricity consumption of FLs compared with that of CFLs and LEDs, but a reduced margin existed between CFLs and LED lamps. It showed that the FLs consumed 681.576 kWh, 1619.466 kWh and 5652.432 kWh, CFLs consumed 387.302 kWh, 692.479 kWh and 1936.600 kWh, while LED consumed 294.188 kWh, 426.608 kWh and 1499.015 kWh. There were significant differences in consumption with 43, 47 and 65% reduction in electricity consumption using CFLs while 57, 73 and 73% using LED fittings were obtained. The CFLs performed better in terms of return on investments by having a lower payback period when compared to LED. The study concluded that replacing FL with CFL and LED would be desirable option in order to enhance optimal performance of lecture theatres, but in terms of payback period, it would be beneficial to retrofit FLs with CFLs.

scholarly journals Far-Red Light Acclimation for Improved Mass Cultivation of Cyanobacteria

Metabolites ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 170 ◽  
Author(s):  
Alla Silkina ◽  
Bethan Kultschar ◽  
Carole A. Llewellyn
Keyword(s):  
Biomass Production ◽  
White Light ◽  
Light Emitting Diode ◽  
Red Light ◽  
Industrial Biotechnology ◽  
Light Conditions ◽  
Light Emitting ◽  
Mass Cultivation ◽  
Chlorogloeopsis Fritschii ◽  
Bioactive Product

Improving mass cultivation of cyanobacteria is a goal for industrial biotechnology. In this study, the mass cultivation of the thermophilic cyanobacterium Chlorogloeopsis fritschii was assessed for biomass production under light-emitting diode white light (LEDWL), far-red light (FRL), and combined white light and far-red light (WLFRL) adaptation. The induction of chl f was confirmed at 24 h after the transfer of culture from LEDWL to FRL. Using combined light (WLFRL), chl f, a, and d, maintained the same level of concentration in comparison to FRL conditions. However, phycocyanin and xanthophylls (echinone, caloxanthin, myxoxanthin, nostoxanthin) concentration increased 2.7–4.7 times compared to LEDWL conditions. The productivity of culture was double under WLFRL compared with LEDWL conditions. No significant changes in lipid, protein, and carbohydrate concentrations were found in the two different light conditions. The results are important for informing on optimum biomass cultivation of this species for biomass production and bioactive product development.

Discomfort glare caused by white LEDs having different spectral power distributions

2017 ◽  
Vol 50 (6) ◽  
pp. 921-936 ◽  
Author(s):  
WJ Huang ◽  
Y Yang ◽  
M Ronnier Luo
Keyword(s):  
White Light ◽  
Spectral Power ◽  
Light Emitting Diode ◽  
Light Emitting ◽  
White Leds ◽  
Significant Difference ◽  
Discomfort Glare ◽  
Led Lights ◽  
Green Leds

This paper describes an experiment to investigate discomfort glare caused by white light-emitting diode (LED) lights having different spectral power distributions. It included two groups: a ‘Metamerism’ group and a ‘correlated colour temperatures (CCT)’ group. In the former group, it was found that white lights at 7000 K constructed from different blue LEDs and the same red and green LEDs gave about the same glare perception. In the latter group, there was a significant difference in glare perception between white lights having different CCTs. Finally, glare models, including unified glare rating (UGR) and the newly derived QUGRspd, and mUGRspd models, were tested using the data from the experiment. All of them gave quite accurate predictions of the data.

scholarly journals Evaluation of the bond strength of orthodontic brackets and the degree of polymerisation and microhardness of an orthodontic adhesive using polyvawe light curing unit and varying light densities

2020 ◽  
Vol 23 (4) ◽  
pp. 9p
Author(s):  
Nuri Mert Topbasi ◽  
Yasin Atakan Benkli
Keyword(s):  
Bond Strength ◽  
Light Emitting Diode ◽  
Control Group ◽  
Bond Failure ◽  
Adhesive Resin ◽  
Mean Values ◽  
Light Emitting ◽  
Significant Difference ◽  
Light Curing

Objective: This study evaluates the shear bond strength (SBS) and Vickers micro-hardness (VHN) of the orthodontic adhesive resin Transbond XT using thermocycled samples. The study also measures the degree of cure by poliwave light curing unit Valo. Materials and Methods: 135 brackets were bonded for the SBS test, and 90 disc-shaped orthodontic adhesive resins were prepared for the VHN test. The measurements were taken either immediately or after 500 or 10.0000 thermocycling. The SBS and VHN test data were statistically evaluated using a Two-way ANOVA and Tukey multiple comparison tests. The degree of conversion (DC) was measured by FTIR. Results: The 10-sec polymerized control group (57.08) and the 10.000 termocycled samples (55.96) had the highest VHN scores. On the other hand, the 3 second polymerized and the 10.000 termocycled samples (39.22) had the lowest VHN scores. There was no significant difference in the bracket SBS values between the termocycled and immediate groups. An evaluation of the bond failure surfaces revealed that the ARI scores did not differ between the immediate and the thermo-cycled groups according to the power modes. The FTIR results revealed that the lowest DC was seen in the 3 second light-cured sample. Conclusion: Transbond XT provided sufficient bonding in terms of microhardness and DC under in vitro bracket bonding conditions after 10 and 8 seconds of Poliwave LED curing. However, low mean values were seen in the groups that received Poliwave LED curing for only 3 seconds. KEYWORDSARI score; FTIR; Light-Emitting Diode; Orthodontics; Thermocycling.

scholarly journals Effectiveness of light emitting diode versus conventional phototherapy in neonatal hyperbilirubinemia: a hospital based observational study

2020 ◽  
Vol 8 (3) ◽  
pp. 1051
Author(s):  
Radheshyam Purkait ◽  
Manik Mondal
Keyword(s):  
Side Effects ◽  
Light Emitting Diode ◽  
Total Duration ◽  
Conventional Group ◽  
Light Sources ◽  
Fluorescent Lamp ◽  
Light Emitting ◽  
Led Phototherapy ◽  
Significant Difference ◽  
Compact Fluorescent Lamp

Background: The most commonly used light sources in the conventional phototherapy units are compact fluorescent lamp (CFL), halogen spotlights and fiberoptic blankets. Recently light emitting diodes (LED) has emerged as better light source for phototherapy and almost replacing all the available conventional light sources. Comparative studies on the efficacy of LED versus conventional phototherapy are limited from India. That is why; this study was undertaken.Methods: 48 neonates of ≥35 weeks gestational age with hyperbilirubinemia were participated in this study. Among them, 24 neonates received conventional phototherapy and rest of them received LED phototherapy. The rate of fall of bilirubin levels at 6 hours and at completion along with total duration of phototherapy in both groups was measured. Results analysed by standard statistical methods.Results: LED phototherapy units showed higher rate of fall in bilirubin at six hrs (LED group: 0.38±0.05 mg/dl/hr versus conventional group: 0.30±0.04 mg/dl/hr, p<0.05) and after completion of therapy (LED group:                      0.32±0.03 mg/dl/hr versus conventional group: 0.26±0.03 mg/dl/hr, p<0.05) compared to conventional group. Significant difference was documented in total duration of phototherapy in LED group (30.8±1.8 hours) when compared to conventional group (34.6±0.7 hours). None of the neonate showed phototherapy failure. Side effects were minimal and comparable in both the groups.Conclusions: The LED phototherapy units are more efficacious in terms of higher rate of fall of bilirubin levels and lesser duration of phototherapy compared to conventional phototherapy units.

scholarly journals Microhardness of resin-based materials polymerized with LED and halogen curing units

2005 ◽  
Vol 16 (2) ◽  
pp. 98-102 ◽  
Author(s):  
Daniela Francisca Gigo Cefaly ◽  
Giovano Augusto de Oliveira Ferrarezi ◽  
Celiane Mary Carneiro Tapety ◽  
José Roberto Pereira Lauris ◽  
Maria Fidela de Lima Navarro
Keyword(s):  
Statistical Analysis ◽  
Light Emitting Diode ◽  
Surface Hardness ◽  
Bottom Surface ◽  
Halogen Lamp ◽  
Light Emitting ◽  
Led Light ◽  
Significant Difference ◽  
Light Curing ◽  
Curing Light

The purpose of this study was to evaluate the microhardness of resin-based materials polymerized with a LED (light-emitting diode) light-curing unit (LCU) and a halogen LCU. Twenty cylindrical specimens (3.0 mm in diameter and 2.0 mm high) were prepared for each tested material (Z100, Definite and Dyract). Specimens were light-cured with two LCUs (Ultraled and Curing Light 2500) for either 40 or 60 s on their top surfaces. Hardness was measured on top and bottom surfaces of each specimen. Statistical analysis was done by ANOVA and Tukey's test (p<0.05). There was no significant difference in hardness between LED LCU and halogen LCU for Z100 and Dyract on top surface. Conversely, lower hardness was recorded when Definite was light-cured with the LED LCU than with the halogen lamp. On bottom surface, hardness was significantly lower for all materials light-cured with LED LCU. Z100 was harder than Dyract and Definite regardless of the light curing unit. There was no significant difference in hardness between the exposure times on top surface. Higher hardness was obtained when the materials were light-cured for 60 s on bottom surface. The tested LED was not able to produce the same microhardness of resin-based materials as the halogen LCU.

scholarly journals Refrigeration temperature enhanced synergistic interaction of curcumin and 460 nm light emitting diode against Staphylococcus saprophyticus at neutral pH

2020 ◽  
Author(s):  
Ziyuan Wang ◽  
Lin Han ◽  
Jie Liu ◽  
Mingfei Yao
Keyword(s):  
Blue Light ◽  
Light Emitting Diode ◽  
Combined Treatment ◽  
Light Dose ◽  
Synergistic Interaction ◽  
Dependent Manner ◽  
Light Emitting ◽  
Neutral Ph ◽  
Staphylococcus Saprophyticus ◽  
Significant Difference

Abstract As considered highly resistant to antibiotics like mecillinam, the rise of Staphylococcus saprophyticus contamination of fresh foods and food processing environments necessitates the development of new antimicrobial approach for food safety control. This study aimed to investigate the synergistic effect of food grade curcumin (CUR) and blue light emitting diode (LED) on S. saprophyticus under different pH (pH 2.2-7.2) and temperatures (4 and 25 °C). The influence of other parameters, including CUR concentration, light dose and incubation time on the inactivation of S. saprophyticus were characterized. Results indicated that the combined treatment of CUR and blue light irradiation significantly (P &lt; 0.05) reduced bacterial counts and the antimicrobial effect was in a CUR concentration and light dose-dependent manner. Moreover, refrigeration temperature (4 °C) significantly (P &lt; 0.05) enhanced the antibacterial effect at neutral pH condition (6.2-7.2), resulting in approximately 6.0 log reductions. Under acidic condition (pH 2.2-5.2), there was no significant difference in bacterial population reduction between treatments at both temperatures. These finding proposed that synergistic interaction of curcumin and 460 nm LED under refrigerated temperature could enhance the inactivation of S. saprophyticus at neutral pH condition.

Influence of Multiple Peak Light-emitting-diode Curing Unit Beam Homogenization Tips on Microhardness of Resin Composites

2020 ◽  
Vol 45 (3) ◽  
pp. 327-338 ◽  
Author(s):  
J Soto-Montero ◽  
G Nima ◽  
FA Rueggeberg ◽  
CTS Dias ◽  
M Giannini
Keyword(s):  
Light Guide ◽  
Profile Analysis ◽  
Light Emitting Diode ◽  
Beam Profile ◽  
Composite Resins ◽  
Bottom Surface ◽  
Light Emitting ◽  
Blue Led ◽  
Significant Difference ◽  
Light Guides

SUMMARY This study evaluated the effect of light curing unit (LCU) guide type (regular or homogenizing) on top and bottom microhardness of conventional and bulk-fill resin-based composites (RBCs). A polywave light-emitting-diode (LED) LCU (Bluephase Style, Ivoclar Vivadent AG) was used with two different light guides: a regular tip (RT, 935 mW/cm2 emittance) and a homogenizer tip (HT, 851 mW/cm2 emittance). Two conventional RBCs (Herculite Ultra [HER], Kerr Corp; Tetric EvoCeram [TEC], Ivoclar Vivadent AG) and two bulk-fill RBCs (SonicFill [SOF], Kerr Corp; Tetric EvoCeram Bulk Fill [TBF], Ivoclar Vivadent AG) were tested. Disc-shaped samples (10 mm Ø), 2-mm thick for conventional composites and 4-mm thick for bulk-fill composites were prepared. Samples were light cured according to manufacturer-recommended times. Knoop microhardness values (KHN) were obtained on the top and bottom surfaces of each specimen at locations correlated with the output of the three LED chips emitting blue (456 nm) or violet light (409 nm). Beam profile analysis using both light guides was also performed. Microhardness of each composite was analyzed using three-way analysis of variance and Tukey honestly significant difference post hoc test (α=0.05). Beam profile images showed better light distribution across the surface of the HT light guide. Use of the HT decreased KHN of HER at the locations of the blue LED chips at bottom of the sample but had no effect on the top surface. For TEC, use of HT increased KHN of all three LED locations at the top surface. Use of the HT increased KHN of SOF at locations corresponding to one of the blue and the violet LED chips at the bottom surface. For TBF, HT increased KHN at all top surface locations. All RBCs showed higher mean KHN at the top compared with the bottom surfaces. In general, all composites presented a higher KHN at the blue LED areas regardless of the surface or the tip used. Results suggest that the homogenizer light guide resulted in significantly increased microhardness at the top, in composite resins containing alternative photoinitiators; however, that effect was not observed at the bottom surfaces.

Which Lighting Option Is the Best for Photography in Rhinoplasty?

2021 ◽  
Author(s):  
Rodrigo Fernández-Pellón ◽  
Meshari Saghir ◽  
Ayman Jaber ◽  
Fazil Apaydin
Keyword(s):  
Light Emitting Diode ◽  
Light Sources ◽  
Light Emitting ◽  
Direct Measurements ◽  
Significant Difference ◽  
Facial Plastic ◽  
The Subject ◽  
Led Lights ◽  
Nasal Length

AbstractPhotography for preoperative analysis and follow-up is indispensable for the facial plastic surgeon. The use of strobe flash units, light-emitting diode (LED) lamps, and their position related to axis of the patient can affect the nasal contours and nasal measurements. The aim of this study was to compare the rhinoplasty pictures taken under three different lighting settings and two different positioning at 30- and 45-degree angles, and with direct measurements taken by caliper from the subjects. Standardized rhinoplasty pictures from 10 patients were taken in frontal view in a studio. These pictures were taken under three different lighting settings: built-in flash of the camera, two strobe flashes, and two LED continuous lights placed at 30 to 45 degree angles to the patient. All the pictures were uploaded to Rhinobase 2.0. In five subjects, direct measurements were done by using a Vernier caliper and compared with the computer measurements. In this study, when comparing the light sources and the angles without taking single flash into account, no relation was found between strobe lights at 30 and 45 degrees and between LED lights at 30 and 45 degrees regarding tip width, base bony width, dorsum width, interalar width, and nasal length. However, a statistically significant difference was found when the angle was changed from 30 to 45 degrees for tip width, interalar width and nasal length. The use of two LED continuous lights or two strobe lights in a studio setting has given similar results. Changing the angles of the light sources from 30 to 45 degrees affected only the tip width and the interalar width; otherwise the rest of the nasal measurements did not show any significant changes. The pictures taken at 45-degree angles to the subject showed the closest values to the direct measurements done on the patient

scholarly journals Effect of Light-Emitting Diode Phototherapy on Allograft Bone After Open Sinus Lift Surgery: A Randomized Clinical Trial (Concurrent Parallel)

2021 ◽  
Vol 12 (1) ◽  
pp. e16-e16
Author(s):  
Mahnaz Arshad ◽  
Zahra Ghanavati ◽  
Pouyan Aminishakib ◽  
Kamran Rasouli ◽  
Gholamreza Shirani
Keyword(s):  
Clinical Trial ◽  
Randomized Clinical Trial ◽  
Light Emitting Diode ◽  
Low Level Laser Therapy ◽  
Control Group ◽  
Sinus Lift ◽  
Light Emitting ◽  
Led Phototherapy ◽  
Significant Difference ◽  
History Of

Introduction: Phototherapy with a light-emitting diode (LED) is used in medicine due to its potential bio-stimulatory effects on the human body. However, controversy still exists regarding the efficacy of low-level laser therapy (LLLT) and phototherapy with LED. This in vivo study aimed to quantitatively and qualitatively assess the newly formed bone following LED phototherapy of the human maxillary sinuses. Methods: This randomized clinical trial (concurrent parallel) was conducted on 44 patients in two groups (n=22) at the Implant Department of Tehran University of Medical Sciences. Randomization was done by a random sequence generator program. The inclusion criteria were absence of chronic sinusitis and chronic bone marrow conditions, no history of surgery at the site, absence of diabetes mellitus, no history of chemotherapy or radiotherapy, maxillary premolar edentulism, and signing informed consent forms. Group A underwent LED phototherapy with 620 ± 2 nm wavelength for 20 minutes daily for a total of 21 days after sinus lift surgery. Group B served as the control group and did not receive phototherapy. After 6 months, the grafted sites were re-opened for implant placement, and bone biopsy samples were obtained using a trephine bur. The samples were stained with hematoxylin and eosin and inspected under a light microscope. The results were statistically analyzed using the Mann-Whitney U test. Both the surgeon and pathologist were blinded to the group allocation of patients. Results: Forty tissue specimens were analyzed. Insignificant differences existed between the two groups in terms of the degree of inflammation, bone quality, and maturity of collagen. Histological analyses revealed no significant difference in the mineralized areas of bone between the two groups (P>0.05). Conclusion: The results indicated that LED phototherapy cannot significantly enhance osteogenesis after sinus lift surgery. No side effects were observed in the experimental group.

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