In vitro degradation and methane production of short-season corn hybrids harvested before or after a light frost

2019 ◽  
Vol 99 (4) ◽  
pp. 741-753
Author(s):  
I.A. Aboagye ◽  
V.S. Baron ◽  
M. Oba ◽  
J. Guyader ◽  
K.A. Beauchemin

In western Canada, short-season corn silage production is increasing due to its potentially high nutritive value. The objective of this study was to determine variability and relationships among nutrient concentration, degradability, and methane (CH4) production of short-season whole-plant corn hybrids harvested before or after light frost (−1.5 °C). Four hybrids, based on their corn heat unit rating (≤2600, CHU rating), were grown in 2 yr in central and southern Alberta (AB) with two field replications. The batch culture and Daisy fermenter techniques were used to characterize degradability and gas production measurements. At both locations, dry matter (DM) concentration was affected by harvest and hybrid (P ≤ 0.02). However, starch and neutral detergent fiber (NDF) concentrations differed (P ≤ 0.01) or tended (P = 0.07) to differ among harvest and hybrid only in central AB. Over both locations and harvest times, CH4production was related negatively to propionate and positively to acetate proportions. In conclusion, harvesting southern AB hybrids after frost increased DM concentration and NDF degradability with no effect on CH4emissions, but the high DM concentration may negatively affect silage quality and animal performance. Harvesting central AB hybrids after frost increased DM and starch concentrations, while reducing CH4emissions but had limited effects on nutrient degradability.

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 459
Author(s):  
Jessie Guyader ◽  
Vern S. Baron ◽  
Karen A. Beauchemin

This study compares yield, nutritive value, and kernel properties of whole plant corn (WPC) harvested before and after a light frost in short growing season areas. Six corn hybrids grown in two years at three locations within Alberta (Canada) were harvested before or after the first frost. Samples of WPC were analyzed for dry matter (DM) content, neutral detergent fiber (NDF) concentration, starch concentration, and 48-h in vitro DM and NDF digestibility (DMD and NDFD, respectively). Cob samples were analyzed for DM, and kernels were analyzed for DM, hardness, particle size distribution, density, and stage of maturity. Delaying harvest to after frost increased DM content of WPC at all locations but exceeded the recommended range (32–38%) in the two warmest locations. Whatever the year and hybrid, DM yield was either not affected or decreased after frost. Postfrost harvest increased starch concentration and modified kernel characteristics only if these were less than expected before frost. Fiber concentration was not affected by harvesting time. Frost had either no impact or increased DMD or NDFD of WPC. We conclude that delaying harvest until after frost in short growing season areas can be beneficial when whole-plant DM content is low before frost.


2018 ◽  
Vol 53 (9) ◽  
pp. 1045-1052
Author(s):  
Mateus Merlo Coelho ◽  
Lúcio Carlos Gonçalves ◽  
José Avelino Santos Rodrigues ◽  
Kelly Moura Keller ◽  
Gustavo Vinícius de Souza dos Anjos ◽  
...  

Abstract: The objective of this work was to evaluate the effects of re-ensiling and bacterial inoculation on the quality of corn silage. The experiment was carried out in a 2x2 factorial design with or without inoculant (association of Lactobacillus plantarum and Propionibacterium acidipropionici), and with re-ensiling after 36 hours of aerobic exposure or only ensiling of the whole plant of 'BRS 1055' corn. The fermentative quality, nutritional parameters, dry matter losses, aerobic stability, and microbiological counts of silages were evaluated. Re-ensiling caused an increase of pH and in acetic acid and propionic acid concentrations, as well as in the dry matter (DM), crude protein, neutral detergent fiber, and neutral detergent fiber crude protein contents. Conversely, there was a reduction in the nonfiber carbohydrates concentration and in in vitro dry matter digestibility for the re-ensiled material. All changes were explained by the higher-effluent production and DM loss of re-ensiled material that was subjected to two compactions. Microbiology was not altered by the treatments. The use of inoculant altered ash content, but it did not influence other parameters. In contrast, re-ensiling after 36 hours of aerobic exposure caused a reduction in the nutritive value of corn silage and accentuated the DM losses.


Agriculture ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 251
Author(s):  
Egon Henrique Horst ◽  
Secundino López ◽  
Mikael Neumann ◽  
Francisco Javier Giráldez ◽  
Valter Harry Bumbieris Junior

The study aimed to examine the effects of harvesting three maize hybrids at different maturity stages on the ruminal fermentation kinetics, fermentation end-products (volatile fatty acid, ammonia and methane) output, and digestibility of maize plant fractions, as well as the degradability of the resulting silage. Three hybrids were compared (Maximus VIP3, Defender VIP and Feroz VIP) harvested at three grain maturity stages (milk (R3), dough (R4) and dent (R5) grain), while silage samples were collected only at the dent grain stage (R5). Grain digestibility tended to decrease as the maturity stage progressed (p < 0.05), while the whole-plant digestibility increased with increasing maturity for the three evaluated hybrids (p < 0.05). The gas production of whole-plant at 24 h of incubation was higher for the Maximus hybrid than for the others (p < 0.05), with average values of 188, 196 and 207 mL g−1 dry matter at stages R3, R4 and R5, respectively. For the in situ ruminal degradation kinetics of silage, instantly degradable dry matter and fiber potential degradability were greater with Maximus maize forage than with the other two hybrids. From the perspective of in vitro results, the Maximus VIP3 hybrid seems to be the most suitable for silage production when harvested between the dough and the dent grain stage of maturity.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 404-405
Author(s):  
Jessica Gusmão ◽  
Marcio Bastos ◽  
Luciana Lima ◽  
Marcus Cardoso ◽  
Thiago Bernardes

Abstract The aim of this study was to assess the nutritive value of snaplage as affected by hybrid and maturity. Five corn hybrids were grown at 70,000 plants/ha on three replicate plots (5 x 5 m) in each of three blocks (21°14′ S, 44°58′ W). Hybrids were harvested at target maturity stage of 40, 35, and 30% of moisture (M1, M2, and M3, respectively) by removing ears from two rows in each plot. Ears were weighed and split into two subsamples. One was split into kernels, husk and shank, and cob, weighed and oven-dried. The second subsample was processed and ensiled in 5-L jars for 90 d. Measurements included dry matter (DM) yield, nutrient analysis and in vitro digestion of neutral detergent fiber (NDF-D, 48h). The experimental design was randomized complete blocks using a mixed repeated-measures model. Data were analyzed using MIXED procedure of SAS, followed by a Tukey test at P ≤ 0.05. Mean moisture at harvest were 42.9, 36.8, and 31.3% for M1, M2, and M3, respectively. The DM yield was unaffected by hybrid and maturity (on average = 19 t/ha). The NDF concentration of husk increased at each maturity (81.5, 83.4, and 83.7% DM, respectively) and NDF-D decreased (45.5, 43.4, and 41.4%, respectively). The NDF concentration of cob increased at each maturity (76.0, 79.5, and 81.6% DM, respectively) and NDF-D decreased (47.3, 43.7, and 38.4%, respectively). Among hybrids, NDF concentration of snaplage varied from 20.9 to 24.5% DM (P = 0.014). The NDF-D of snaplage decreased at each maturity (Figure 1; P = 0.001). The starch concentration of snaplage increased from M1 to M2, but no difference between M2 and M3 (P = 0.009). Snaplage has a wide harvest window; however, changes in the nutritive value of snaplage as affected by maturity should be considered when formulating diets.


Author(s):  
Behlül Sevim ◽  
Tugay Ayaşan ◽  
İsmail Ülger ◽  
Şerife Ergül ◽  
Sait Aykanat ◽  
...  

This study was conducted to determine the nutritive value of malt barley varieties using in vitro gas production technique and in vitro methane production. Atılır, Durusu and Fırat were used as three malt barley varieties. As a result of this study, between the varieties in terms of dry matter (DM), crude ash (CA), crude protein (CP), crude fat (CF), neutral detergent fiber (NDF) and hemicellulose (HEM) were found significantly important but differences between the varieties in terms of ADF were not found significant. The gas production rate of malt barley varieties ranged from 64.00 to 72.50 ml/200 mg DM. The metabolisable energy (ME) and net energy lactation (NEL) contents of malt barley varieties ranged from 11.75 to 12.86 MJ/kg DM and 7.16 to 7.98 MJ/kg DM respectively. The highest methane (CH4) content was obtained from the variety of Fırat with a value of 13.34 ml/200 mg DM, whereas the lowest value was obtained from the Atılır variety with a value of 12.16 ml/200 mg DM.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1653
Author(s):  
Jone Ibarruri ◽  
Idoia Goiri ◽  
Marta Cebrián ◽  
Aser García-Rodríguez

This research aimed to evaluate in vitro organic matter digestibility, fermentation characteristics and methane production of fruit and vegetable discards processed by solid state fermentation (SSF) by Rhizopus sp. Mixtures were composed of approximately 28% citric fruits, 35% other fruits and 37% vegetables. Fruit and vegetables were processed and fermented to obtain a stabilized product. Nutritional characterization and in vitro ruminal fermentation tests were performed to determine the effect of fungal bioconversion on digestibility, end products and gas production kinetics. Results indicate that SSF reduced organic matter and reducing sugars, while it increased crude protein and neutral detergent fiber, acid detergent fiber and neutral detergent insoluble protein. The in vitro gas production showed that SSF led to a reduction of the organic matter digestibility (p < 0.001), short chain fatty acids (SCFA; p = 0.003) and CH4 (p = 0.002). SSF reduced the gas production from the insoluble fraction (p = 0.001), without modifying the production rate (p = 0.676) or the lag time (p = 0.574). Regarding SCFA profile, SSF increased acetic (p = 0.020) and decreased propionic (p = 0.004) and butyric (p = 0.006) acids proportions, increasing acetic to propionic (p = 0.008) and acetic plus butyric to propionic (p = 0.011) ratios. SSF succeeded in obtaining a stabilized material enriched in protein, but at the expense of a reduction of protein availability and organic matter digestibility. These changes should be considered before including them in a ruminant’s rations.


Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1854
Author(s):  
Yulin Ma ◽  
Xu Chen ◽  
Muhammad Zahoor Khan ◽  
Jianxin Xiao ◽  
Shuai Liu ◽  
...  

The current study was conducted to explore the ammoniation treatment effects on the chemical composition and in vitro digestibility of rice straw in Chinese Holsteins. For this purpose, rice straw was stored in polyethylene bags (35 × 25 cm, 350 g per bag) including (i) no additives (RS); (ii) 5% urea (5U, dry matter (DM) basis); (iii) 9% corn steep liquor + 5% urea (9C5U, DM basis); (iv) 9C2.5U; and (v) 9C2.5U + 3% molasses (9C2.5U3M, DM basis). The air-dry matter of the mixture was kept at the same level at 55% for all treatments. Fifteen bags (5 treatments × 3 repeats) were prepared and stored at ambient temperature (25 ± 3 °C). The chemical composition and in vitro digestibility were measured at day 60 after storage. Our analysis revealed that all the four ammoniation treatments improved the in vitro DM and neutral detergent fiber (IVNDFD) digestibility. In addition, all the four ammoniation treatments significantly (P < 0.001) increased the levels of crude protein (CP), gas production (GP), acetic acid (AA), butyric acid (BA) and total volatile fatty acid (TVFA) contents of the rice straw and decreased the neutral detergent fiber (NDF) and acid detergent fiber (ADF) of the rice straw compared to the control. Within four treated groups, the 9C5U treatment was most effective. Finally, we concluded that ammoniation treatments increased the nutritive value of rice straw. In addition the 9C5U treatment could be an effective ammoniation treatment for the better utilization of rice straw.


Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.


Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2212
Author(s):  
Mónica Gandarillas ◽  
Juan Pablo Keim ◽  
Elisa María Gapp

Background: Horses are hindgut fermenters, and it is therefore important to determine the postgastric nutritive value of their feedstuffs and diets. Moreover, it has been demonstrated in other animal species that the fermentation of diets results in different values than those expected from pure ingredients. Therefore, the general objective of this work is to evaluate the gas production (GP) and volatile fatty acid (VFA) concentration, as well as the associative effects, of mixtures of different forages and concentrated foods, which are representative of the traditional diets of high-performance horses. Methods: An in vitro gas production experiment was conducted to assess the fermentation of two forages and three concentrates that are typical in horse diets. The combination of 70% of forage and 30% concentrates was also assessed to determine potential associative effects. Results: Concentrates and grains produced higher GP and VFA than forages when evaluated alone. When experimental diets were incubated, GP parameters and VFA concentrations of forage–concentrate mixtures had unexpected differences from the values expected from the fermentation of pure ingredients, suggesting the occurrence of associative effects. Conclusions: Our results indicate that there is a need to evaluate the fermentation of diets, rather than predicting from the values of pure ingredients.


1998 ◽  
Vol 22 ◽  
pp. 238-240
Author(s):  
L. R. Ndlovu ◽  
L. Hove

Browse species are important food resources in semi-arid areas, especially during the dry season when the nutritive value of grass is at its lowest. However, browse plants often contain secondary plant compounds which limit their nutritive value. Proanthocyanidins (PAs) (also called condensed tannins) and related flavonoids are a common constituent of woody plants in tropical regions (Mangan, 1988). PAs cause a bitter and astringent taste which lowers food palatability and they also lower the digestibility of proteins and carbohydrates (Jacksonet al., 1996). PAs also interfere with current chemical methods that are used for estimating nutritive value of foods (Reed, 1995). Biological assays, especially ,in vitrotechniques, have a potential to reflect better the nutritive value of foods that contain PAs. Thein vitrogas production technique has been found to reliably predict the nutritive value of temperate forages (Makkaret al., 1996). There has been limited research on its efficacy with tropical forages. The experiment reported here was conducted to test the hypothesis that gas production of tropical browse species reflects their content of fibre, protein and/or PAs.


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