scholarly journals Comparative studies of meat quality traits and the proteome profile between low pH and high pH muscles in longissimus dorsi of Berkshire

2017 ◽  
Author(s):  
Sivakumar Subramaniyan ◽  
Da Rae Kang ◽  
Young- Chul Jung ◽  
Jong Hyun Jung ◽  
Yang- Il Choi ◽  
...  
Keyword(s):  
Meat Quality ◽  
Comparative Studies ◽  
Low Ph ◽  
Longissimus Dorsi ◽  
Quality Traits ◽  
High Ph ◽  
Meat Quality Traits ◽  
Proteome Profile

scholarly journals Analysis of FTO and PLIN2 Polymorphisms in Relation to Carcass and Meat Quality Traits in Pigs

2019 ◽  
Vol 19 (1) ◽  
pp. 71-83 ◽  
Author(s):  
Daniel Polasik ◽  
Eva-Maria Kamionka ◽  
Mirosław Tyra ◽  
Grzegorz Żak ◽  
Arkadiusz Terman
Keyword(s):  
Meat Quality ◽  
Fat Content ◽  
Longissimus Dorsi ◽  
Quality Traits ◽  
Large White ◽  
Meat Quality Traits ◽  
Pcr Rflp ◽  
Perilipin 2 ◽  
Water Holding ◽  
Intramuscular Fat Content

AbstractThe aim of this study was to analyze the association of polymorphisms in alpha-ketoglutarate-dependent dioxygenase (FTO) and perilipin 2 (PLIN2) genes with carcass and meat quality traits in pigs reared in Poland. The research covered 578 sows that belong to the following breeds: Duroc, Hampshire, Polish Landrace, Pietrain, Puławska and Polish Large White. FTO (FM244720:g.400C>G) and PLIN2 (GU461317:g.98G>A) genes variants were determined by means of PCR-RFLP and ACRS-PCR methods respectively. Association between individual genotypes and analyzed traits was calculated by means of GLM procedure for Polish Landrace, Polish Large White and Puławska breeds separately and for all six breeds together in case of FTO gene. The results showed that FTO variants were associated with weight of loin without backfat and skin (WL), loin eye area (AL) and meat percentage (MP) in Polish Large White (P≤0.05), mean backfat thickness from 5 measurements (BFT) and pH measured 45 min after slaughter in m. longissimus dorsi (pH24 ld) as well as with water holding-capacity (WHC) in Puławska breed (P≤0.01). PLIN2 genotypes, however were correlated with WL and height of the loin eye (HL) in Polish Large White and Puławska (P≤0.05), AL in Polish Large White (P≤0.01) as well as luminosity (L*) in Puławska (P≤0.05) pigs. We observed most consistent relationships of PLIN2 SNP with intramuscular fat content (IMF) and WHC. In 3 analyzed breeds GG genotype was connected with highest values of these traits (P≤0.05).

Genetic parameters of carcass and meat quality traits in different muscles (longissimus dorsi and semimembranosus) of Hanwoo (Korean cattle)1

2017 ◽  
Vol 95 (8) ◽  
pp. 3359-3369 ◽  
Author(s):  
M. S. A. Bhuiyan ◽  
H. J. Kim ◽  
D. H. Lee ◽  
S. H. Lee ◽  
S. H. Cho ◽  
...  
Keyword(s):  
Meat Quality ◽  
Genetic Parameters ◽  
Longissimus Dorsi ◽  
Quality Traits ◽  
Korean Cattle ◽  
Meat Quality Traits

scholarly journals Identification of expression QTL (eQTL) of genes expressed in porcine M. longissimus dorsi and associated with meat quality traits

BMC Genomics ◽  
2010 ◽  
Vol 11 (1) ◽  
Author(s):  
Siriluck Ponsuksili ◽  
Eduard Murani ◽  
Manfred Schwerin ◽  
Karl Schellander ◽  
Klaus Wimmers
Keyword(s):  
Meat Quality ◽  
Longissimus Dorsi ◽  
Quality Traits ◽  
Expression Qtl ◽  
Meat Quality Traits

scholarly journals Identification of eQTLs and sQTLs associated with meat quality in beef

2019 ◽  
Author(s):  
Joel David Leal Gutierrez ◽  
Mauricio A. Elzo ◽  
Raluca G. Mateescu
Keyword(s):  
Meat Quality ◽  
Fixed Effects ◽  
Differential Expression Analysis ◽  
Cytoskeletal Proteins ◽  
Longissimus Dorsi ◽  
Quality Traits ◽  
Master Regulators ◽  
Longissimus Dorsi Muscle ◽  
Meat Quality Traits ◽  
Dorsi Muscle

Abstract Background: Transcription has a substantial genetic control and genetic dissection of gene expression could help us understand the genetic architecture of complex phenotypes such as meat quality in cattle. The objectives of the present research were: 1) to perform eQTL and sQTL mapping analyses for meat quality traits in longissimus dorsi muscle; 2) to uncover genes whose expression is influenced by local or distant genetic variation; 3) to identify expression and splicing hot spots; and 4) to uncover genomic regions affecting the expression of multiple genes. Results: Eighty steers were selected for phenotyping, genotyping and RNA-seq evaluation. A panel of traits related to meat quality was recorded in longissimus dorsi muscle. Information on 112,042 SNPs and expression data on 8,588 autosomal genes and 87,770 exons from 8,467 genes were included in an expression and splicing quantitative trait loci (QTL) mapping (eQTL and sQTL, respectively). A gene, exon and isoform differential expression analysis previously carried out in this population identified 1,352 genes, referred to as DEG, as explaining part of the variability associated with meat quality traits. The eQTL and sQTL mapping was performed using a linear regression model in the R package Matrix eQTL. Genotype and year of birth were included as fixed effects, and population structure was accounted for by including as a covariate the first PC from a PCA analysis on genotypic data. The identified QTLs were classified as cis or trans using 1 Mb as the maximum distance between the associated SNP and the gene being analyzed. A total of 8,377 eQTLs were identified, including 75.6% trans, 10.4% cis, 12.5% DEG trans and 1.5% DEG cis; while 11,929 sQTLs were uncovered: 66.1% trans, 16.9% DEG trans, 14% cis and 3% DEG cis. Twenty-seven expression master regulators and 13 splicing master regulators were identified and were classified as membrane-associated or cytoskeletal proteins, transcription factors or DNA methylases. These genes could control the expression of other genes through cell signaling or by a direct transcriptional activation/repression mechanism. Conclusion: In the present analysis, we show that eQTL and sQTL mapping makes possible positional identification of gene and isoform expression regulators.

scholarly journals Level of Fatty Acids, Selected Quality Traits of Longissimus Dorsi and Semimembranosus Muscles and Their Relationship with Fattening and Slaughter Performance in Polish Landrace Pigs

2014 ◽  
Vol 14 (2) ◽  
pp. 417-427 ◽  
Author(s):  
Grzegorz Żak ◽  
Marek Pieszka ◽  
Władysław Migdał
Keyword(s):  
Fatty Acids ◽  
Meat Quality ◽  
Saturated Fatty Acids ◽  
Quality Parameters ◽  
Longissimus Dorsi ◽  
Quality Traits ◽  
Meat Quality Traits ◽  
Slaughter Performance ◽  
Soxhlet Method ◽  
Meat Samples

Abstract The aim of this study was to perform a comprehensive analysis of the level of fatty acids and selected meat quality traits, as well as their association with fattening and slaughter performance in Polish Landrace pigs. The study was conducted for two muscles: musculus longissimus dorsi and musculus semimembranosus. The study used 100 Polish Landrace gilts. The experimental animals originated from nucleus farms located in Poland. Their parents were animals with a known status of the RYR 1 genotype (all animals were dominant homozygotes). Pigs were fattened, slaughtered and evaluated postmortem in piggeries of the Experimental Stations of the National Research Institute of Animal Production using test station procedures. After reaching 100 kg body weight, pigs were slaughtered and their half-carcasses dissected. Fattening and slaughter parameters were determined. Meat samples were analysed for crude fat using the Soxhlet method. The composition of higher fatty acids was determined by gas chromatography. Research results indicate that saturated fatty acids found in m. longissimus dorsi and m. semimembranosus were positively correlated to n-3 and n-6 PUFA . A similarly high correlation was observed between the level of UFA and n-6 and n-3 PUFA in the loin and ham. The fact that the proportions of some acids (e.g. n-6/n-3 PUFA ) fail to meet WHO standards requires making efforts to improve them. Likewise, selection work is necessary to improve IMF levels in meat. The low correlations between meat quality traits and fattening and slaughter performance suggest that quality parameters should be regarded as independent traits in pig improvement models. In order to limit meat quality traits in pig improvement models, it is necessary to make use of the correlations between them

scholarly journals Meat quality traits and proteome profile of woody broiler breast (pectoralis major) meat

2018 ◽  
Vol 97 (1) ◽  
pp. 337-346 ◽  
Author(s):  
K. Cai ◽  
W. Shao ◽  
X. Chen ◽  
Y.L. Campbell ◽  
M.N. Nair ◽  
...  
Keyword(s):  
Meat Quality ◽  
Pectoralis Major ◽  
Quality Traits ◽  
Meat Quality Traits ◽  
Proteome Profile ◽  
Broiler Breast
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