scholarly journals Unrestrictive identification of post-translational modifications in Hevea brasiliensis latex

2018 ◽  
Vol 96 (6) ◽  
pp. 818-824 ◽  
Author(s):  
Mohd Afiq Hazlami Habib ◽  
Chee-Yuen Gan ◽  
Aishah Abdul Latiff ◽  
Mohd Nazri Ismail
Keyword(s):  
Hevea Brasiliensis ◽  
Electron Transfer Dissociation ◽  
Rubber Particle ◽  
Natural Rubber Latex ◽  
Modern Society ◽  
Collision Induced Dissociation ◽  
Lysine Acetylation ◽  
Rubber Latex ◽  
Post Translational Modifications ◽  
The Stability

The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in modern society. Post-translational modifications (PTMs) of the latex proteins are important for the stability and functionality of the proteins. In this study, latex proteins were acquired from the C-serum, lutoids, and rubber particle layers of latex without using prior enrichment steps; they were fragmented using collision-induced dissociation (CID), higher-energy collisional dissociation (HCD), and electron-transfer dissociation (ETD) activation methods. PEAKS 7 were used to search for unspecified PTMs, followed by analysis through PTM prediction tools to crosscheck both results. There were 73 peptides in 47 proteins from H. brasiliensis protein sequences derived from UniProtKB were identified and predicted to be post-translationally modified. The peptides with PTMs identified include phosphorylation, lysine acetylation, N-terminal acetylation, hydroxylation, and ubiquitination. Most of the PTMs discovered have yet to be reported in UniProt, which would provide great assistance in the research of the functional properties of H. brasiliensis latex proteins, as well as being useful biomarkers. The data are available via the MassIVE repository with identifier MSV000082419.

scholarly journals Proteomics analysis of latex from Hevea brasiliensis (clone RRIM 600)

2017 ◽  
Vol 95 (2) ◽  
pp. 232-242 ◽  
Author(s):  
Mohd Afiq Hazlami Habib ◽  
Gan Chee Yuen ◽  
Fazilah Othman ◽  
Nurul Nabilah Zainudin ◽  
Aishah Abdul Latiff ◽  
...  
Keyword(s):  
Hevea Brasiliensis ◽  
Electron Transfer Dissociation ◽  
De Novo ◽  
Draft Genome ◽  
Natural Rubber Latex ◽  
Modern Society ◽  
Biosynthetic Pathways ◽  
Genome Database ◽  
Mass Spectral ◽  
Latex Biosynthesis

The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today’s modern society. Following ultracentrifugation, the latex can be separated into 3 layers: C-serum, lutoids, and rubber particles. Previous studies have shown that a large number of proteins are present in these 3 layers. However, a complete proteome for this important plant is still unavailable. Protein sequences have been recently translated from the completed draft genome database of H. brasiliensis, leading to the creation of annotated protein databases of the following H. brasiliensis biosynthetic pathways: photosynthesis, latex allergens, rubberwood formation, latex biosynthesis, and disease resistance. This research was conducted to identify the proteins contained within the latex by way of de novo sequencing from mass spectral data obtained from the 3 layers of the latex. Peptides from these proteins were fragmented using collision-induced dissociation, higher-energy collisional dissociation, and electron-transfer dissociation activation methods. A large percentage of proteins from the biosynthetic pathways (63% to 100%) were successfully identified. In addition, a total of 1839 unique proteins were identified from the whole translated draft genome database (AnnHBM).

Surface nanostructure of Hevea brasiliensis natural rubber latex particles

2011 ◽  
Vol 390 (1-3) ◽  
pp. 157-166 ◽  
Author(s):  
Kanjanee Nawamawat ◽  
Jitladda T. Sakdapipanich ◽  
Chee C. Ho ◽  
Yujie Ma ◽  
Jing Song ◽  
...  
Keyword(s):  
Natural Rubber ◽  
Hevea Brasiliensis ◽  
Natural Rubber Latex ◽  
Rubber Latex ◽  
Latex Particles ◽  
Surface Nanostructure

Characterization of Lidocaine Transdermal Patches from Natural Rubber Latex

2013 ◽  
Vol 747 ◽  
pp. 103-106 ◽  
Author(s):  
Prapaporn Boonme ◽  
Hasleena Boontawee ◽  
Wirach Taweepreda ◽  
Wiwat Pichayakorn
Keyword(s):  
Drug Release ◽  
Natural Rubber ◽  
Physicochemical Properties ◽  
Rubber Tree ◽  
Natural Rubber Latex ◽  
Rubber Latex ◽  
Transdermal Patches ◽  
The Stability ◽  
Main Component

The mucous liquid of Hevea brasiliensis or Para rubber tree, called natural rubber latex (NRL), composes of cis-1,4-polyisoprene which can form a patch under suitable formulation. In this study, blank and 5% lidocaine-loaded NRL patches were formulated and then characterized for physicochemical properties as well as evaluated in vitro drug release and stability. The patches were observed for their appearances. Surface morphology of the patches was investigated using a SEM. XRD was employed to study the crystallinity of the drug, the patch, and the drug-loaded patch. The extractions of lidocaine-loaded patches were analyzed for drug contents by HPLC. In vitro drug release study was performed using modified Franz diffusion cells. The patches at initial preparation and after kept at 4, 25, and 45 °C for 3 months were investigated for the stability determination. The results suggested that NRL could be used as a main component in pharmaceutical transdermal patches with acceptable physicochemical properties. Lidocaine-loaded NRL patches provided desirable drug release but high storage temperatures could age the patches resulting in darken color and lower release amount.

Allergenicity of Hev b 13, a major esterase allergen in natural rubber latex (Hevea brasiliensis) allergy, does not only depend on its carbohydrate moiety

2010 ◽  
Vol 47 (4) ◽  
pp. 871-877 ◽  
Author(s):  
Pierre Rougé ◽  
Raphaël Culerrier ◽  
Marion Campistron ◽  
Claude Granier ◽  
Françoise Bienvenu ◽  
...  
Keyword(s):  
Natural Rubber ◽  
Hevea Brasiliensis ◽  
Natural Rubber Latex ◽  
Carbohydrate Moiety ◽  
Rubber Latex

scholarly journals Angiogenic properties of natural rubber latex biomembranes and the serum fraction of Hevea brasiliensis

2009 ◽  
Vol 39 (3) ◽  
pp. 564-569 ◽  
Author(s):  
Mariselma Ferreira ◽  
Ricardo José Mendonça ◽  
Joaquim Coutinho-Netto ◽  
Marcelo Mulato
Keyword(s):  
Natural Rubber ◽  
Hevea Brasiliensis ◽  
Natural Rubber Latex ◽  
Rubber Latex
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