A modified method for isolating mouse islets of an adequate quality, quantity, and purity

2017 ◽  
Vol 95 (4) ◽  
pp. 455-458 ◽  
Author(s):  
Jiejie Xu ◽  
Baogang Peng ◽  
Caiyun Zhang ◽  
Jiwei Xu ◽  
Yi Ma ◽  
...  
Keyword(s):  
Isolation Procedure ◽  
Isolated Islets ◽  
Diabetes Research ◽  
Islet Isolation ◽  
High Quality ◽  
Modified Method ◽  
Adequate Quality ◽  
Mouse Islets ◽  
Gradient Separation ◽  
Density Gradient Separation

Mouse islets are widely used in diabetes research. Thus an adequate quality, quantity, and purity of islets are needed for high-quality investigations. We performed a combination of filtration and density gradient separation and optimized many steps in the islet isolation procedure, including perfusion, digestion, and purification. Our results show that an increased quality, quantity, and purity of isolated islets can be achieved using these modifications. Moreover, this method can guarantee maximal recovery and purity of the isolated islets and is easy to perform with practice.

A modified method for labeling human platelets with indium-111 oxine using albumin density-gradient separation.

Radiology ◽  
1982 ◽  
Vol 145 (1) ◽  
pp. 219-221 ◽  
Author(s):  
R W Bunting ◽  
R J Callahan ◽  
S Finkelstein ◽  
R S Lees ◽  
H W Strauss
Keyword(s):  
Density Gradient ◽  
Human Platelets ◽  
Modified Method ◽  
Indium 111 ◽  
Gradient Separation ◽  
Density Gradient Separation

GAIN Premix Facility: An Innovative Approach for Improving Access to Quality Vitamin and Mineral Premix in Fortification Initiatives

2012 ◽  
Vol 33 (4_suppl3) ◽  
pp. S381-S389 ◽  
Author(s):  
Philippe Guinot ◽  
Vincent Jallier ◽  
Alessandro Blasi ◽  
Christophe Guyondet ◽  
Marc Van Ameringen
Keyword(s):  
Large Scale ◽  
Edible Oils ◽  
Quality Standards ◽  
Private Industry ◽  
Target Groups ◽  
High Quality ◽  
Adequate Quality ◽  
Standards And Guidelines ◽  
Set Up ◽  
The Cost

Background Vitamin and mineral premix is one of the most significant recurring input costs for large-scale food fortification programs. A number of barriers exist to procuring adequate quality premix, including accessing suppliers, volatile prices for premix, lack of quality assurance and monitoring of delivered products, and lack of funds to purchase premix. Objective To develop and test a model to procure premix through a transparent and efficient process in which an adequate level of quality is guaranteed and a financial mechanism is in place to support countries or specific target groups when there are insufficient resources to cover the cost of premix. Methods Efforts focused on premixes used to fortify flour, such as wheat or maize (iron, zinc, B vitamins, and vitamin A), edible oils (vitamins A and D), and other food vehicles, such as fortified complementary foods, complementary food supplements, and condiments. A premix procurement model was set up with three distinct components: a certification process that establishes industry-wide standards and guidelines for premix, a procurement facility that makes premix more accessible to countries and private industry engaged in fortification, and a credit facility mechanism that helps projects finance premix purchases. Results After three years of operation, 15 premix suppliers and 29 micronutrient manufacturers have been certified, and more than US$23 million worth of premix that met quality standards has been supplied in 34 countries in Africa, Central and Southern Asia, and Eastern Europe, reaching an estimated 242 million consumers. Conclusions The Premix Facility demonstrated its effectiveness in ensuring access to high-quality premixes, therefore enabling the success of various fortification programs.

scholarly journals Зберігання селекційних форм маточних коренеплодів моркви посівної

2013 ◽  
pp. 12-13
Author(s):  
В. В. Хареба ◽  
О. В. Хареба ◽  
О. В. Позняк
Keyword(s):  
Plant Breeding ◽  
Research Institutions ◽  
High Quality ◽  
Northern Forest ◽  
Breeding Work ◽  
Modified Method ◽  
Root Crops ◽  
Carrot Seed ◽  
Quality Stock

Розглянуто модифікований спосіб зберігання ма-точних коренеплодів селекційних форм моркви посів-ної, в основі якого – скорочення втрат коренеплодів під час зимового зберігання та отримання чисто-сортного насіння заданих комбінацій у процесі селек-ційної роботи. Розглянутий модифікований спосіб зберігання маточних коренеплодів моркви посівної безпосередньо у полі перспективний для освоєння в науково-дослідних установах та насінницьких госпо-дарствах у зонах Північного Лісостепу та Полісся України. The modified method of storage of uterine root crops of plant-breeding forms of sowing carrot was considered; in its basis is reduction of root crops’ losses during winter storage and receipting of high-quality stock seed from the set combinations in the process of plant-breeding work. The considered modified method of storage of uterine carrot seed directly in the field is perspective for development in research institutions and seed farms in areas of the Northern Forest and Woodlands of Ukraine.

scholarly journals An improved method for preparation of mouse retinal cryosections

2020 ◽  
Vol 64 (3) ◽  
Author(s):  
Ling Li ◽  
Yongqiong Lin ◽  
Fang Yang ◽  
Tongdan Zou ◽  
Jialiang Yang ◽  
...  
Keyword(s):  
Retinal Detachment ◽  
Vision Research ◽  
New Method ◽  
Specific Marker ◽  
Improved Method ◽  
High Quality ◽  
New Methods ◽  
Modified Method ◽  
Routine Assay ◽  
Super Glue

Immunohistochemistry using mouse retinal cryosections is a routine assay used in vision research. However, retinal tissues are fragile, and it is difficult to obtain an ideal retinal cryosection. Here, we developed a modified method for preparing retinal cryosection. Super Glue was applied on the surface of the sclera before the cornea and the lens are removed from either the unfixed or PFA-fixed mouse eyeballs. The new methods largely prevented retinal detachment in mouse retinal cryosections. Immunostaining of retinal cryosections derived from PFA-fixed mouse eyes using rod and cone markers yielded high-quality immunofluorescent images. Immunolabeling of retinal cryosections obtained from unfixed mouse eyes using a cilium-specific marker had improved orientations of photoreceptor connecting cilia. This new method substantially improves the morphology and immunostaining results of fixed and unfixed mouse eyes.

Assessment of Islet Isolation Efficacy in Dogs

1994 ◽  
Vol 3 (1) ◽  
pp. 91-101 ◽  
Author(s):  
Michael P.M. Van Der Burg ◽  
Onno R. Guicherit ◽  
Marijke Frölich ◽  
Johannes P. Scherft ◽  
Frans A. Prins ◽  
...  
Keyword(s):  
Isolated Islets ◽  
Insulin Content ◽  
Islet Isolation ◽  
Size Distributions ◽  
Large Variability ◽  
Factors Affecting ◽  
Density Separation ◽  
Islet Volume ◽  
Dog Pancreas ◽  
Density Gradient Purification

The outcome of islet isolation is considered uncertain because of the large variability of islet and insulin yield, but comparison of the isolated and native islet population has not been attempted. We therefore addressed the efficacy of collagenase digestion, and density gradient purification of islets from the splenic dog pancreas (n = 31) by morphometry of the islet volume and size distribution, and by extraction of insulin and amylase, in samples from the pancreas, the digest, and gradient fractions. In contrast to a ~90% recovery of pancreatic insulin and amylase after digestion, islet yield amounted to 50% of the islet content of the pancreas. After density separation, islets were mainly found in the purified fractions, while half of the recovered insulin was located in the acinar fraction of the gradients — indicating a substantial proportion of islets entrapped in acinar fragments. The islet and insulin content of the pancreas correlated well with islet and insulin yield after digestion (r = 0.7, p < .0001). The insulin content of digest suspensions did neither correlate with islet nor insulin recovery in the purified fraction of the gradients (r = 0.4) as opposed to the islet content of digest suspensions, which correlated with both (r = 0.7, p < .0001). After density separation near 100% purity was obtained, and no loss of insulin from isolated islets was demonstrated by extraction and microscopy. Size distributions of native and isolated islets demonstrated no fragmentation. We conclude that the variability of isolation outcome may be attributed to a large extent to the variability of the native endocrine pancreas. Isolation efficacy was best documented by morphometry, because insulin extraction did not discriminate between free and entrapped islets. However, assessment by both morphometry and extraction allowed the quantitation of entrapped islets, and demonstrated preservation of β-cell granulation. Similar studies should facilitate the analysis of other factors affecting islet isolation in man.

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