Retinol binding protein 4 affects the adipogenesis of porcine preadipocytes through insulin signaling pathways

2013 ◽  
Vol 91 (4) ◽  
pp. 236-243 ◽  
Author(s):  
Jia Cheng ◽  
Zi-Yi Song ◽  
Lei Pu ◽  
Hao Yang ◽  
Jia-Meng Zheng ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Insulin Signaling ◽  
Binding Protein ◽  
Mammalian Target Of Rapamycin ◽  
Suppressive Effect ◽  
Retinol Binding Protein ◽  
Serine Threonine Kinase ◽  
Retinol Binding Protein 4 ◽  
Threonine Kinase

Retinol binding protein 4 (RBP4), a novel cytokine, is mainly secreted by hepatocytes and adipocytes. RBP4 reportedly induces insulin resistance and RBP4 secretion is increased in the adipocytes of animals or humans with type 2 diabetes, obesity, and metabolic syndrome, but its role in preadipocyte differentiation remains unclear. In this study, we investigated the effect of RBP4 on the differentiation of porcine preadipocytes into adipocytes. The results suggest that RBP4 significantly suppresses the differentiation of porcine preadipocytes into adipocytes, including those treated with the hormone cocktail methylisobutylxanthine–dexamethasone–insulin. RBP4 also weakened the activity of normal threonine 308, the phosphorylation of serine/threonine kinase AKT, and downstream insulin signaling, including the mammalian target of rapamycin (mTOR) and β-catenin. Moreover, the activation of insulin signaling mediated by knockdown RBP4 in porcine preadipocytes was recovered in the suppression of LY294002. RBP4 also had a suppressive effect on the differentiation of porcine preadipocytes by decreasing the activation of insulin signaling pathways.

scholarly journals Retinol binding protein 4 and incident diabetes – the Atherosclerosis Risk in Communities Study (ARIC Study)

2013 ◽  
Vol 16 (2) ◽  
pp. 388-397 ◽  
Author(s):  
Vivian C. Luft ◽  
Mark Pereira ◽  
James S. Pankow ◽  
Christie Ballantyne ◽  
David Couper ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Incident Diabetes ◽  
Parental History ◽  
Nonesterified Fatty Acids ◽  
Atherosclerosis Risk In Communities ◽  
Retinol Binding Protein 4 ◽  
Atherosclerosis Risk

Background: Retinol binding protein 4 (RBP4) has been described as a link between impaired glucose uptake in adipocytes and systemic insulin sensitivity. Objective: To determine whether RBP4 fasting levels predict the development of type 2 diabetes. Methods: Using a case-cohort design, we followed 543 middle-aged individuals who developed diabetes and 537 who did not over ~9 years within the population-based Atherosclerosis Risk in Communities Study. Weighted Cox proportional hazards analyses permitted statistical inference of the RBP4 – incident diabetes associations to the entire cohort. Results: Women in the highest tertile of RBP4 presented greater risk of developing diabetes (HR = 1.74; 95%CI 1.03 – 2.94) in analyses adjusted for age, ethnicity, study center, parental history of diabetes, hypertension, glomerular filtration rate, body mass index, waist-hip ratio, nonesterified fatty acids, adiponectin, leptin, triglycerides and HDL-C. When additionally adjusted for fasting insulin, this association’s significance became borderline (HR = 1.68; 95%CI 1.00 – 2.82). No association between RBP4 levels and incident diabetes was found in men. Conclusion: These findings suggest that RBP4 levels may be directly involved in the pathogenesis of type 2 diabetes in women.

scholarly journals An Increase in Serum Retinol-Binding Protein 4 in the Type 2 Diabetic Subjects with Nephropathy

2009 ◽  
Vol 56 (2) ◽  
pp. 287-294 ◽  
Author(s):  
Miho MURATA ◽  
Tomoyuki SAITO ◽  
Taeko OTANI ◽  
Masami SASAKI ◽  
Aki IKOMA ◽  
...  
Keyword(s):  
Binding Protein ◽  
Retinol Binding Protein ◽  
Serum Retinol ◽  
Retinol Binding Protein 4 ◽  
Type 2 Diabetic

Retinol binding protein 4 and its membrane receptors: a metabolic perspective

2015 ◽  
Vol 22 (1) ◽  
Author(s):  
Ronja Fedders ◽  
Matthias Muenzner ◽  
Michael Schupp
Keyword(s):  
Metabolic Diseases ◽  
Binding Protein ◽  
Current Data ◽  
Membrane Receptors ◽  
Retinol Binding Protein ◽  
Metabolic Effects ◽  
Retinol Binding Protein 4 ◽  
Underlying Mechanisms

AbstractNearly a decade of intense research has passed since the first report linking circulating retinol binding protein 4 (RBP4) to the development of insulin resistance. By now, a variety of underlying mechanisms have been identified; some of them are adherent to the canonical role of this circulating protein, which is to transport and deliver retinol to target tissues, and others that seem rather independent of retinol transport. Despite all these efforts, a consensus in the basic principles of RBP4’s metabolic effects has not been reached and some controversy remains. Using this as an opportunity, we here review and discuss current data on RBP4’s action on insulin sensitivity and its dependency on retinol homeostasis. We pay special attention to the involvement of RBP4 membrane receptors that were identified during these years, such as ‘stimulated by retinoic acid 6’ (STRA6), and whose identification added another layer of complexity to RBP4’s diverse actions. A better understanding of RBP4’s functions might allow its therapeutic exploitations, urgently needed in our period that is defined by an epidemic increase in metabolic diseases such as obesity and type 2 diabetes.

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