Pollen morphology in Lyperia (Scrophulariaceae) and related taxa with a multivariate analysis of the major patterns of pollen character variation in tribe Manuleae

2000 ◽  
Vol 78 (2) ◽  
pp. 208-220
Author(s):  
Charles L Argue
Keyword(s):  
Principal Components ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Cross Sectional ◽  
Representative Species ◽  
Pollen Characters ◽  
Character Variation ◽  
Components Analysis ◽  
Pollen Character ◽  
Scanning Electron

The pollen of all six species of Lyperia (Scrophulariaceae, tribe Manuleae) was investigated by light microscopy and scanning electron microscopy of whole and fractured grains. Pollen grains in Lyperia antirrhinoides (L.f.) Hilliard, Lyperia formosa Hilliard, Lyperia tenuiflora Benth., and Lyperia violacea (Jarosz) Benth. differed from those in Lyperia tristis (L.f.) Benth. and Lyperia lychnidea (L.) Druce in number and length of colpi, sculpturing of the colpus margin, reduction of apocolpial lumina size, reduction of exine thickness toward the colpi, ornamentation of the outer nexine surface, arrangement of columellae beneath the muri, direction of cross-sectional compression in elliptical muri, and relative dimensions of the muri and columellae. Pollen grains from 50 representative species of the putatively related genera Sutera and Jamesbrittenia were also examined. Correlations among quantitative pollen characters of tribe Manuleae and the Sutera complex were investigated by principal components analysis. Morphological similarities in the pollen of Lyperia, genera of the Sutera complex, and other Manuleae were summarized by ordination of the principal components data and (or) cluster analysis. The pollen grains of L. lychnidea and L. tristis differed from those of other Lyperia and stood apart from those of Jamesbrittenia, Sutera, and all other Manuleae. Pollen morphology in Sutera and Jamesbrittenia intergrades with that in tricolporate species of Lyperia.Key words: pollen morphology, Scrophulariaceae, Lyperia, Jamesbrittenia, Sutera.

A biometrie and taxonomic study of pollen character variation in Berendtiella and Hemichaena (Scrophulariaceae)

1983 ◽  
Vol 61 (1) ◽  
pp. 53-62 ◽  
Author(s):  
Charles L. Argue
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Preliminary Evaluation ◽  
Taxonomic Study ◽  
Type Ii ◽  
Pollen Data ◽  
Pollen Characters ◽  
Close Relationship ◽  
Character Variation ◽  
Pollen Character

The pollen grains of Berendtiella and Hemichaena are single, isopolar, and tricolporate with a microreticulate to reticulate sexine 2. Intra- and inter-specific variability in pollen characters are assessed statistically, and preliminary evaluation of the taxonomic utility of these data is presented. The bearing of the pollen evidence on proposed intratribal categories and the transfer of the genera from the tribe Scrophularieae (Cheloneae) to the tribe Mimuleae are discussed. The proposed close relationship between the genera and the transfer of Diplacus rugosa to Berendtiella are consistent with the pollen data, but suggested affinities between Berendtiella and Diplacus clearly are not. The grains of Berendtiella and Hemichaena resemble the type II pollen of Mimulus, and the uniformity of pollen morphology in members of the Mimuleae other than Mimulus lends support to the recognition of segregate genera in the latter palynologically diverse genus.

scholarly journals Pollen morphology of section Cheirolepis Boiss. of the genus Centaurea L. (Asteraceae) in Turkey

2019 ◽  
Vol 26 (2) ◽  
pp. 131-148
Author(s):  
Burcu Yilmaz Çitak ◽  
Hüseyin Dural ◽  
Tuna Uysal ◽  
Nur Münevver Pinar
Keyword(s):  
Electron Microscope ◽  
Clustering Analysis ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Base Length ◽  
Diagnostic Features ◽  
Spine Length ◽  
Pollen Characters ◽  
Pollen Shape ◽  
Scanning Electron

In this paper, the palynomorphology of 17 taxa of section Cheirolepis in Turkey, were investigated by light (LM) and scanning electron microscope (SEM). Detailed descriptions of the pollen grains were given for each taxon and a well-resolved dendrogram was generated through numerical analysis of palynological diagnostic features. The pollen grains were found to be radially symmetric, isopolar, and generally 3-zonocolporate, with the exception of C. derderiifolia, C. kotschyi var. floccosa, and C. saligna, which were also 4-zonocolporate. The shape of the pollen grains were prolatespheroidal, with the polar axes of 32.76–46.26 μm and equatorial axes of 31.86–45.82 μm. The sculpturing of the pollen grains was generally scabrate-perforate or rarely microechinate-perforate. The spines were conical with a changing base length. The length and the width of the spines varied between 0.48 and 2.28 μm and 0.4 and 3.39 μm, respectively. The number of perforations at the base of the spines ranged in two or morerows and they varied between 5 and 30. The number of spines was 16–70 in 10 μm2. The results of this study showed that the polar axes, equatorial axes, aperture type, pollen shape, spine length, perforation number, and number of spines in 10 μm2 are essential for distinguishing the studied taxa. The taxa were grouped by clustering analysis of selected pollen characters using the UPGMA method.

scholarly journals Systematic relevance of pollen morphology in tribe Hylocereeae (Cactaceae)

PhytoKeys ◽  
2019 ◽  
Vol 128 ◽  
pp. 121-140 ◽  
Author(s):  
Catalina Ruiz-Domínguez ◽  
Andrew P. Vovides ◽  
Victoria Sosa
Keyword(s):  
Light Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Pollen Grain ◽  
Area Index ◽  
Oblate Spheroidal ◽  
Representative Species ◽  
Pollen Characters ◽  
Polar Area

Hylocereeae is one of the nine tribes in the subfamily Cactoideae (Cactaceae), for which the limits and recognition of genera have been controversial. Essentially, this group comprises epiphytic and hemiepiphytic genera with stems modified as climbing structures. The aim of this paper is to examine pollen attributes in representative species of genera of Hylocereeae, focusing on Selenicereus whose current circumscription comprises Hylocereus and three Weberocereus species, to find whether significant potentially apomorphic and/or autapomorphic systematic characters can be discovered. Utilizing SEM and light microscopy, 25 pollen characters were observed and measured. Tribe Hylocereeae is stenopalynous, with pollen grains isopolar and radially symmetrical monads, mostly tricolpate, except in Kimnachia, Pseudoripsalis and Weberocereus, whose pollen grains are pantocolpate. Seven attributes (five qualitative and two continuous) exhibited useful variation and were coded. The character of brevicolpate pollen grains was shared by Kimnachia ramulosa and Pseudorhipsalis amazonica. Convex quadrangular outline in the polar view was shared by Weberocereus tunilla and S. glaber. The absence of spinules on the exine was shared by S. minutiflorus and S. stenopterus. The largest pollen grain, found in Selenicereus megalanthus, might be correlated with polyploidy. Selenicereus is the taxon with the highest variation in pollen attributes, including species with an exine with or without spinules and variable polar area index and shape (subprolate or oblate-spheroidal).

scholarly journals Pollen morphological study on some rare Allium L. (Amaryllidaceae) taxa in Turkey

2019 ◽  
Vol 26 (1) ◽  
pp. 47-55
Author(s):  
Birol Başer ◽  
Mehmet Firat ◽  
Riza Binzet
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Study ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Bilateral Symmetry ◽  
Short Axis ◽  
Pollen Characters ◽  
Scanning Electron

The pollen morphology of 10 Allium L. taxa, 6 of which are endemic to Turkey, were investigated in detail by light and scanning electron microscopy. According to LM and SEM, the pollen grains of genera were monad, monosulcate percolate, heteropolar with bilateral symmetry, 25.30 to 53,85 µm long axis (LA) and 17.55 to 36.86 µm short axis (SA), the form was prolate (mean of LA/SA ratio 1.30 to 1.70 and in polar view boat-shaped. Three types of ornamentation were determined. Striate-rugulate-perforate type in Allium longisepalum, A. oreophilum, A. anacoleum, A. microspathum, A.shirnakiense, A. purpureoviride and A. armenum, Rugulate–perforate type in A. pervariense and A. gabardagense and Rugulate–reticulate-perforate type in A. arlgirdense. Sulcus membrane ornamentations were rugulate or psilate. The sulcus extends from the distal to proximal ends in A. anacoleum, A. arlgirdense and A. pervariense. The present study on some Turkish species of Allium showed that several morphological pollen characters may possess taxonomical value.

Evolution of Angiosperm Pollen: 8. Lamiids

2020 ◽  
Vol 105 (3) ◽  
pp. 323-376
Author(s):  
Li-E Yang ◽  
Lu Lu ◽  
Kevin S. Burgess ◽  
Hong Wang ◽  
De-Zhu Li
Keyword(s):  
Maximum Likelihood ◽  
Pollen Morphology ◽  
Pollen Size ◽  
State Transitions ◽  
Character State ◽  
Evolutionary Mechanisms ◽  
Pollen Characters ◽  
Exine Ornamentation ◽  
Shape Class ◽  
Pollen Character

Lamiids, a clade composed of approximately 15% of all flowering plants, contains more than 50,000 species dispersed across 49 families and eight orders (APG IV, 2016). This paper is the eighth in a series that analyzes pollen characters across angiosperms. We reconstructed a maximum likelihood tree based on the most recent phylogenetic studies for the Lamiids, comprising 150 terminal genera (including six outgroups) and covering all eight orders and 49 families within the clade. To illustrate pollen diversity across the Lamiids, pollen grains from 22 species (22 genera in 14 families) were imaged under light, scanning, and transmission electron microscopy. Eighteen pollen characters that were documented from previous publications, websites, and our new observations were coded and optimized onto the reconstructed phylogenetic tree using Fitch parsimony, maximum likelihood, and hierarchical Bayesian analysis. Pollen morphology of the Lamiids is highly diverse, particularly in shape class, pollen size, aperture number, endoaperture shape, supratectal element shape, and tectum sculpture. In addition, some genera show relatively high infrageneric pollen variation within the Lamiids: i.e., Coffea L., Jacquemontia Choisy, Justicia L., Pedicularis L., Psychotria L. nom. cons., Sesamum L., Stachytarpheta Vahl, and Veronica L. The plesiomorphic states for 16 pollen characters were inferred unambiguously, and 10 of them displayed consistent plesiomorphic states under all optimization methods. Seventy-one lineages at or above the family level are characterized by pollen character state transitions. We identified diagnostic character states for monophyletic clades and explored palynological evidence to shed light on unresolved relationships. For example, palynological evidence supports the monophyly of Garryales and Metteniusaceae, and sister relationships between Icacinaceae and Oncothecaceae, as well as between Vahliales and Solanales. The evolutionary patterns of pollen morphology found in this study reconfirm several previously postulated evolutionary trends, which include an increase in aperture number, a transition from equatorially arranged apertures to globally distributed ones, and an increase in exine ornamentation complexity. Furthermore, there is a significant correlation between pollen characters and a number of ecological factors, e.g., pollen size and pollination type, pollen ornamentation and pollination type, and shape class and plant growth form. Our results provide insight into the ecological, environmental, and evolutionary mechanisms driving pollen character state changes in the Lamiids.

scholarly journals Pollen characters as taxonomic evidence in some species of Dipsacaceae from Iran

2017 ◽  
Vol 24 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Ebadi-Nahari Mostafa ◽  
Nikzat-Siahkolaee Sedigheh ◽  
Eftekharian Rosa
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Pollen Type ◽  
Upgma Tree ◽  
Pollen Characters ◽  
Exine Ornamentation ◽  
The Family ◽  
The World ◽  
Plant Taxon

Pollen morphology of nine species representing four genera: Cephalaria Schrad, Dipsacus L., Pterocephalus Vaill. and Scabiosa L. of the family Dipsacaceae in Iran has been investigated by means of scanning electron microscopy (SEM). The results showed that pollen grains were triporate and tricolpate. The pollen type of Scabiosa rotata Bieb. (tri- and tetraporate) is the first report in the world. The sizes of pollen grains fall into the classification group magna (pollen grain diameter 50–100 μm). Pollen shapes vary from preoblate to prolate and their polar views were triangulate and lobate. The exine ornamentation varies from gemmate in S. rotata to spinulate in the rest studied species. Species of Scabiosa have been dispersed in UPGMA tree that this confirmed the previous studies about taxonomic problems and species complexity in this genus. These results show the transfer of the some Scabisoa species to Lomelosia Raf. based on palynological characters. Pollen morphology of the family is helpful at the generic and specific level.Bangladesh J. Plant Taxon. 24(2): 129–136, 2017 (December)

scholarly journals Pollen morphology of Iranian Dracocephalum L. (Lamiaceae) and its taxonomic significance

2015 ◽  
Vol 22 (2) ◽  
pp. 99-110 ◽  
Author(s):  
Maryam Naderifar ◽  
Ali Sonboli ◽  
Abbas Gholipour
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Taxonomic Significance ◽  
Infrageneric Classification ◽  
Exine Ornamentation ◽  
Plant Taxon ◽  
Scanning Electron

Pollen morphology of 11 Iranian Dracocephalum L. species was investigated using light and scanning electron microscopy to evaluate their taxonomic significance for the infrageneric classification of the genus. Pollen grains of all examined taxa were isopolar, hexacolpate, circular in polar view and spheroidal to prolate in equatorial view (P/E = 1.0?2.0). The smallest pollen grains were observed in D. aucheri (P = 29.7 ?m, E = 22.6 ?m), while the largest pollen was found in D. lindbergii (P = 45.1 ?m, E = 33.7 ?m). The highest and lowest apocolpium index (AI) were measured in D. aucheri (AI = 0.27) and D. surmandinum (AI = 0.08), respectively. Colpus membrane was egranulate in all examined species except for D. multicaule and D. ghahremanii. The main exine ornamentation type was characterized as bireticulate including five different subtypes. The results revealed that the exine ornamentation is a diagnostic character useful for the classification of Dracocephalum.Bangladesh J. Plant Taxon. 22(2): 99-110, 2015 (December)

scholarly journals Pollen morphology of the genus Eremanthus Less. (Vernonieae, Asteraceae)

2012 ◽  
Vol 26 (1) ◽  
pp. 46-57 ◽  
Author(s):  
BenoÎt Loeuille ◽  
Raquel Maria Batista Souza-Souza ◽  
Vanessa Holanda Righetti Abreu ◽  
Cláudia Barbieri Ferreira Mendonça ◽  
Vania Gonçalves-Esteves
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Additional Data ◽  
Phylogenetic Reconstruction ◽  
Pollen Grains ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Quantitative Characters ◽  
Scanning Electron

In order to evaluate the significance of the pollen morphology for generic and infrageneric taxonomy of the genus Eremanthus (Vernonieae, Asteraceae), and to provide additional data for its phylogenetic reconstruction, the pollen of 20 of the 23 species of the genus was examined using light and scanning electron microscopy. Acetolysed pollen grains were measured, described, and illustrated using light microscopy, while non-acetolysed pollen grains were analyzed using scanning electron microscopy. Pollen grains of these species are isopolar, oblate-spheroidal in most of the species, more rarely prolate spheroidal or suboblate, subtriangular amb, tricolporate and subechinolophate. The variation among quantitative characters does not correlate with the macromorphological subdivision of the genus or with the generic or specific limits.

scholarly journals Pollen morphology of Ukrainian species of the genus Pedicularis L. (Orobanchaceae Vent.)

2012 ◽  
Vol 24 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Olena M. Peregrym ◽  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Taxonomic Revision ◽  
Palynological Data ◽  
First Time ◽  
Scanning Electron

Abstract Pollen grains of 10 species of Pedicularis occurring in Ukraine were investigated using light and scanning electron microscopy. Palynological data on 6 species are reported for the first time. General pollen morphology of the genus Pedicularis is presented. Three types of apertures (2-syncolpate, 3-colpate, and 3-syncolpate) and seven subtypes of sculpture (3-syncolpate pilate, 3-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate, 2-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate-tubeculate-perforate, 2-syncolpate microfoveolate, and 2-syncolpate microscabrate-perforate) are indentified. The subgenus Pedicularis is heterogeneous in its types of apertures and sculpture of the surface, which indicates the need of further taxonomic revision of the group.

scholarly journals Características palinológicas de las araliáceas de México

2017 ◽  
pp. 117 ◽  
Author(s):  
Victoria Sosa
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Pollen Grains ◽  
Point Of View ◽  
Generic Level ◽  
Dichotomous Key ◽  
Pollen Characters ◽  
Scanning Electron

This work studies 17 species of mexican Araliaceae from the palynological point of view belonging to the genera Aralia, Dendropanax, Didymopanax, Redera, Oreopanax y Sciadodendron . All the pollen descriptions are presented at the generic level. The grains were studied based in scanning electron microscope and light microscope. It includes a dichotomous key for the genera grains. The results showed few differences between the pollen grains of the genera although some exine characters are important. Redera and Didymopanax presented an exine semi-tectate and the rest of the genera had tectate-perforate grains. This paper compared also some pollen characters with morphological ones, finding pollen characters considered primitive correlated with characters of primitive Araliaceae groups .

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