Genetic characterization of MexicanFrankiastrains nodulatingCasuarina equisetifolia

1999 ◽  
Vol 77 (9) ◽  
pp. 1214-1219
Author(s):  
Néstor-Octavio Pérez ◽  
Hiram Olivera ◽  
Luis Vásquez ◽  
María Valdés
Keyword(s):  
Nifh Gene ◽  
Single Copy ◽  
Bacterial Strains ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Ribosomal Operon ◽  
Clonal Cultures ◽  
Typing Methods ◽  
Polymerase Chain ◽  
Native Strains

There is a need to increase the utilization of the Casuarina equisetifolia J.R. Forst. & G. Forst. - Frankia symbiosis and be sure of its effectiveness in Mexico. This may be facilitated by selecting appropriate bacterial strains for which ecological characteristics are known. We tested various typing methods to develop genetic markers for ecological studies. DNA, extracted from clonal cultures of native strains or from reference cultures of Casuarina-infective Frankia strains, was used as the template in polymerase chain reactions (PCR) with primers targeting different DNA regions. nifH and 16S rDNA probes from the reference strain Frankia Br were utilized to authenticate the isolates. Polymorphisms of the restricted fragments of the intergenetic spacer between the 16S-23S rDNAs were analyzed. Repetitive extragenic palindromic sequences (rep-PCR) (BOXA1R primer) were used to generate genomic fingerprints. All studied strains showed two copies of the ribosomal operon and a single copy of the nifH gene. PCR - restriction fragment length polymorphism patterns of the 16S-23S intergenetic spacer (IGS) were similar for all Frankia isolates; however, the rep-PCR technique was sensitive enough to distinguish between some of these Frankia strains. The Mexican cultured strains of Frankia nodulating C. equisetifolia appeared to be closely related to the isolated and nodular Frankia from trees growing outside Australia.Key words: Frankia, Casuarina, 16S rRNA, 16S-23S IGS, nifH, repetitive sequence polymerase chain reaction.

scholarly journals Investigation of Lethal Concurrent Outbreak of Chlamydiosis and Pigeon Circovirus in a Zoo

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1654
Author(s):  
Wei-Tao Chen ◽  
Chin-Ann Teng ◽  
Cheng-Hsin Shih ◽  
Wei-Hsiang Huang ◽  
Yi-Fan Jiang ◽  
...  
Keyword(s):  
Disease Outbreaks ◽  
Chlamydia Psittaci ◽  
Bursa Of Fabricius ◽  
Chain Reactions ◽  
Renal Epithelium ◽  
Polymerase Chain Reactions ◽  
Transmission Electron ◽  
Intracytoplasmic Inclusion Bodies ◽  
Polymerase Chain ◽  
Streptopelia Orientalis

During the spring, an outbreak of sudden death involving 58 birds occurred in a zoo. Histopathological examinations revealed variable numbers of intracytoplasmic basophilic microorganisms in the macrophages, hepatocytes, and renal epithelium of most birds, along with occasional botryoid intracytoplasmic inclusion bodies within histiocytes in the bursa of Fabricius. Based on the results of histopathological examinations, immunohistochemical staining, transmission electron microscopy, and polymerase chain reactions, genotype B Chlamydia psittaci infection concurrent with pigeon circovirus (PiCV) was diagnosed. A retrospective survey, including two years before the outbreak and the outbreak year, of C. psittaci and PiCV infections of dead birds in the aviaries, revealed that the outbreak was an independent episode. The findings of this study indicate that concurrent infection with C. psittaci and PiCV might lead to lethal outbreaks of chlamydiosis, particularly Streptopelia orientalis. In addition, persistently monitoring both pathogens and identifying potential PiCV carriers or transmitters might also help prevent lethal disease outbreaks.

Investigation of Mutations in Exon 12 Of MYH7, 16 Of β-MYBPC3 and 2 Of TCAP Gene in Dogs with Dilated Cardiomyopathy using PCR-SSCP Technique

2021 ◽  
Author(s):  
R.B. Vishnurahav ◽  
S. Ajithkumar ◽  
Usha Narayana Pillai ◽  
N. Madhvan Unny ◽  
K.D. John Martin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dilated Cardiomyopathy ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Multifunctional Protein ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Intron 1 ◽  
Cardiac Disorders ◽  
Polymerase Chain

Background: Dilated cardiomyopathy is the important myocardial disease and one of the most common cause of death in the medium to large size dog breeds worldwide. The disease is characterized by dilatation of cardiac chambers and thinning of walls leads to systolic failure. Mutations in some sarcomere genes leads to cardiomyopathy in humans. Sarcomere is an important multifunctional protein network involved in the signal reception and transduction. Mutations in β-MYH7, MYBPC3 and TCAP genes produce alterations in the morphology of heart (hypertrophy or dilatation).Methods: In this study twenty apparently healthy and twenty five dogs with dilated cardiomyopathy (DCM) were selected from patients reported or referred to University Veterinary Hospital and Teaching Veterinary Clinical Complex, Mannuthy (2015-2017) based on the clinical examination, radiographic, electrocardiographic, haematobiochemical and echocardiographic studies cardiac disorders (Dilated cardiomyopathy and hypertrophic cardiomyopathy) were confirmed.Result: In the present study we investigated genetic alterations of exon 12 of MYH7, 16 of β-MYBPC3 and 2 of TCAP gene in dogs by polymerase chain reaction -single stranded confirmation of polymorphism (PCR-SSCP). Polymerase chain reactions were analysed using acrylamide gel and samples with different pattern of bands were sequenced. Polymerase chain reaction-SSCP showed different migration of band pattern in the intron 1 of TCAP gene in one sample.

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