Physical mapping of 45S rRNA genes in Cucumis species by fluorescence in situ hybridization

1999 ◽  
Vol 77 (3) ◽  
pp. 389-393 ◽  
Author(s):  
Jin-Feng Chen ◽  
Jack E Staub ◽  
Jeffrey W Adelberg ◽  
Jiming Jiang
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cucumis Sativus ◽  
Rrna Genes ◽  
Phylogenetic Distance ◽  
Rdna Sites ◽  
Cucumis Species ◽  
Cucumis Hystrix ◽  
26S Rrna

The chromosomal locations of the genes coding for the 18S-5.8S-26S rRNA was investigated in Cucumis species using fluorescence in situ hybridization. Cucumber (Cucumis sativus L., 2n = 2x = 14) possesses four pairs of rDNA loci that were mapped to chromosomes 1C, 2C, 4C, and 7C. The distinctive hybridization sites of the 18S-5.8S-26S rRNA genes provide several useful cytogenetic markers for identification of chromosomes in C. sativus. The 18S-5.8S-26S rDNA genes have also been detected on two chromosome pairs, one major and one minor pair of loci, in melon (Cucumis melo L., 2n = 2x = 24) and on three pairs of Cucumis hystrix Chakr. chromosomes. The different number and pattern of rDNA sites is consistent with the hypothesis that considerable phylogenetic distance exists among these species.Key words: fluorescence in situ hybridization, 45S rRNA gene, cytogenetics, Cucumis sativus, Cucucmis melo, Cucumis hystrix.

Physical mapping of the 18S–5.8S–26S rRNA genes in barley by in situ hybridization

Genome ◽  
1992 ◽  
Vol 35 (6) ◽  
pp. 1013-1018 ◽  
Author(s):  
I. J. Leitch ◽  
J. S. Heslop-Harrison
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Gene Mapping ◽  
Chromosome 1 ◽  
Rrna Genes ◽  
C Banding ◽  
Rdna Sites ◽  
Group 2 ◽  
26S Rrna

The 18S–5.8S–26S rRNA genes have been located on five pairs of barley (Hordeum vulgare L., 2n = 2x = 14) chromosomes (in descending order of copy number, barley chromosome numbers 6, 7, 5, 1, and 2; homoeologous groups 6I, 5I, 1I, 7I, and 2I) by in situ hybridization followed by C-banding. All sites were at intercalary positions. The pairs of major sites on chromosomes 6 (6I)1 and 7 (5I) are well known. Silver staining of nuclei and meiotic analysis have previously indicated that additional rDNA sites may be present, but the presence of sites on a further three chromosome pairs was unexpected. Within the tribe Triticeae, few species have more than two pairs of rDNA sites, and they have not been reported on group 2 chromosomes. We propose calling the new sites Nor-I1 (on chromosome 5 (1I)), Nor-I4 (on chromosome 1 (7I)), and Nor-I5 (on chromosome 2 (2I)), and that any further rDNA sites on homoeologous group 2 chromosomes should be called Nor-5. As conventional, all designations are based on temporal order of discovery in the Triticeae and designating the H. vulgare genome as I. In situ hybridization is valuable for gene mapping, since it can detect the presence of genes with a very wide range of copy numbers at different sites.Key words: C-banding, nucleolus organizer regions, fluorescent in situ hybridization, Hordeum vulgare, gene mapping.

scholarly journals A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH)

2009 ◽  
Vol 55 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Ke Bi ◽  
James P. Bogart ◽  
Jinzhong Fu
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dispersal Ability ◽  
Habitat Isolation ◽  
45S Rdna ◽  
Isolated Populations ◽  
Ambystoma Jeffersonianum ◽  
Rdna Sites ◽  
Rdna Polymorphism

Abstract The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a probe (FISH-rDNA). Our results revealed the presence of rDNA polymorphism among A. jeffersonianum populations in terms of number, location and FISH signal intensity on the chromosomes. Nine rDNA cytotypes were found in ten geographically isolated populations and most of them contained derivative rDNA sites. Our preliminary study provides strong indication of karyotypic diversification of A. Jeffersonianum that is demonstrated by intraspecific variation of 45S rDNA cytotypes. rDNA cytotype polymorphism has been described in many other caudate amphibians. We predict that habitat isolation, low dispersal ability and decline of effective population size could facilitate the fixation and accumulation of variable rDNA cytotypes during their chromosome evolution.

Characterization of Thinopyrum distichum chromosomes using double fluorescence in situ hybridization, RFLP analysis of 5S and 26S rRNA, and C-banding of parents and addition lines

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 689-696 ◽  
Author(s):  
A Fominaya ◽  
S. Molnar ◽  
G. Fedak ◽  
K. C. Armstrong ◽  
N.-S. Kim ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome Pair ◽  
Triticum Turgidum ◽  
5S Rrna ◽  
Polymorphism Analysis ◽  
Addition Lines ◽  
C Banding ◽  
26S Rrna

Diagnostic markers for eight Thinopyrum distichum addition chromosomes in Triticum turgidum were established using C-banding, in situ hybridization, and restriction fragment length polymorphism analysis. The C-band karyotype conclusively identified individual Th. distichum chromosomes and distinguished them from chromosomes of T. turgidum. Also, TaqI and BamHI restriction fragments containing 5S and 18S–5.8S–26S rRNA sequences were identified as positive markers specific to Th. distichum chromosomes. Simultaneous fluorescence in situ hybridization showed both 5S and 18S–5.8S–26S ribosomal RNA genes to be located on chromosome IV. Thinopyrum distichum chromosome VII carried only a 18S–5.8S–26S rRNA locus and chromosome pair II carried only a 5S rRNA locus. The arrangement of these loci on Th. distichum chromosome IV was different from that on wheat chromosome pair 1B. Two other unidentified Th. distichum chromosome pairs also carried 5S rRNA loci. The homoeologous relationship between Th. distichum chromosomes IV and VII and chromosomes of other members of the Triticeae was discussed by comparing results obtained using these physical and molecular markers.Key words: Triticum turgidum, homoeologous relationship, Triticeae, addition lines, NOR.

scholarly journals Karyotype analysis and visualization of 45S rRNA genes using fluorescence in situ hybridization in aroids (Araceae)

2015 ◽  
Vol 9 (2) ◽  
pp. 145-160 ◽  
Author(s):  
Katrijn Van Laere ◽  
Prabhu Shankar Lakshmanan ◽  
Tom Eeckhaut ◽  
Johan Van Huylenbroeck ◽  
Erik Van Bockstaele ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Karyotype Analysis ◽  
Rrna Genes

Distribution of 45S rDNA in Modern Rose Cultivars (Rosa hybrida), Rosa rugosa, and Their Interspecific Hybrids Revealed by Fluorescence in situ Hybridization

2016 ◽  
Vol 149 (3) ◽  
pp. 226-235 ◽  
Author(s):  
Xiao-Liu Ding ◽  
Ting-Liang Xu ◽  
Jing Wang ◽  
Le Luo ◽  
Chao Yu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Interspecific Hybrids ◽  
Evolutionary Dynamics ◽  
The Other ◽  
45S Rdna ◽  
Expected Number ◽  
Rdna Sites ◽  
Rdna Loci

To elucidate the evolutionary dynamics of the location and number of rDNA loci in the process of polyploidization in the genus Rosa, we examined 45S rDNA sites in the chromosomes of 6 modern rose cultivars (R. hybrida), 5 R. rugosa cultivars, and 20 hybrid progenies by fluorescence in situ hybridization. Variation in the number of rDNA sites in parents and their interspecific hybrids was detected. As expected, 4 rDNA sites were observed in the genomes of 4 modern rose cultivars, while 3 hybridization sites were observed in the 2 others. Two expected rDNA sites were found in 2 R. rugosa cultivars, while in the other 3 R. rugosa cultivars 4 sites were present. Among the 20 R. hybrida × R. rugosa offspring, 13 carried the expected number of rDNA sites, and 1 had 6 hybridization sites, which exceeded the expected number by far. The other 6 offspring had either 2 or 3 hybridization sites, which was less than expected. Differences in the number of rDNA loci were observed in interspecific offspring, indicating that rDNA loci exhibit instability after distant hybridization events. Abnormal chromosome pairing may be the main factor explaining the variation in rDNA sites during polyploidization.

Chromosomal mapping of mouse 5S rRNA genes by direct R-banding fluorescence in situ hybridization

1994 ◽  
Vol 66 (4) ◽  
pp. 246-249 ◽  
Author(s):  
Y. Matsuda ◽  
K. Moriwaki ◽  
V.M. Chapman ◽  
Y. Hoi-Sen ◽  
J. Akbarzadeh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
5S Rrna ◽  
Chromosomal Mapping ◽  
Rrna Genes ◽  
5S Rrna Genes

scholarly journals α-Proteobacterial Symbionts of Marine Bryozoans in the Genus Watersipora

2006 ◽  
Vol 73 (1) ◽  
pp. 303-311 ◽  
Author(s):  
Christine M. Anderson ◽  
Margo G. Haygood
Keyword(s):  
Phylogenetic Analysis ◽  
In Situ Hybridization ◽  
16S Rrna ◽  
Fluorescence In Situ Hybridization ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Bacterial Symbionts ◽  
California Coast ◽  
Specific Fluorescence

ABSTRACT Bacterial symbionts that resembled mollicutes were discovered in the marine bryozoan Watersipora arcuata in the 1980s. In this study, we used PCR and sequencing of 16S rRNA genes, specific fluorescence in situ hybridization, and phylogenetic analysis to determine that the bacterial symbionts of “W. subtorquata” and “W. arcuata” from several locations along the California coast are actually closely related α-Proteobacteria, not mollicutes. We propose the names “Candidatus Endowatersipora palomitas” and “Candidatus Endowatersipora rubus” for the symbionts of “W. subtorquata” and “W. arcuata,” respectively.

Sign in / Sign up

Export Citation Format

Share Document