Molecular biogeography and evolution of the Microthlaspi perfoliatum s.l. polyploid complex (Brassicaceae): chloroplast DNA and nuclear ribosomal DNA restriction site variation

1998 ◽  
Vol 76 (3) ◽  
pp. 382-396 ◽  
Author(s):  
Marcus Koch ◽  
Klaus Mummenhoff ◽  
Herbert Hurka
Keyword(s):  
Molecular Phylogeny ◽  
Chloroplast Dna ◽  
Ribosomal Dna ◽  
Species Complex ◽  
Restriction Site ◽  
Natural Populations ◽  
Nuclear Ribosomal Dna ◽  
Polyploid Complex ◽  
Northern Iran ◽  
Ploidy Levels

The genusThlaspi L. s.l. comprises numerous segregate lineages, which have been recognized as single genera. One of these, Microthlaspi, represents one such segregate. It consists of morphologically similar annual species. The species have different ploidy levels (2x, 4x, 6x) and are usually summarized under the designation Thlaspi perfoliatum agg. The assumed ancestral diploid members of the species complex, Microthlaspi granatense (Boiss. & Reut.) F.K. Meyer, Microthlaspi umbellatum (Steven ex DC.) F.K. Meyer, and Microthlaspi natolicum (Boiss.) F.K. Meyer, are restricted to northwestern Africa, southeastern Spain and Turkey, northern Iran, and Lebanon. Microthlaspi perfoliatum (L.) F.K. Meyer is widely distributed all over Europe and shows three ploidy levels. Diploid M. perfoliatum is restricted to Middle Europe, whereas tetraploid and hexaploid M. perfoliatum are distributed all over Europe. Individual plants from 125 natural populations throughout the geographic range were analysed using chloroplast DNA (cpDNA) and nuclear ribosomal DNA (nrDNA) restriction site analysis. Within Microthlaspi, 31 cpDNA restriction site mutations, six nrDNA restriction site variations, and two length mutations in the IGS region of the nrDNA were found, and a molecular phylogeny of the species complex has been derived. Polymorphisms in molecular data partitioned cytotypes of M. perfoliatum. The geographical distribution patterns apparently reflect ancient speciation processes and postglacial vegetation history.Key words: chloroplast DNA, nuclear ribosomal DNA, Microthlaspi perfoliatum polyploid complex, molecular phylogeny, biogeography.

Molecular phylogenetics of Thlaspi s.l. (Brassicaceae) based on chloroplast DNA restriction site variation and sequences of the internal transcribed spacers of nuclear ribosomal DNA

1997 ◽  
Vol 75 (3) ◽  
pp. 469-482 ◽  
Author(s):  
Klaus Mummenhoff ◽  
Andreas Franzke ◽  
Marcus Koch
Keyword(s):  
Chloroplast Dna ◽  
Ribosomal Dna ◽  
Restriction Site ◽  
Nuclear Ribosomal Dna ◽  
Hybrid Origin ◽  
Ploidy Levels ◽  
Restriction Site Variation ◽  
Its Regions ◽  
Site Variation ◽  
Dna Restriction

Systematics of the genus Thlaspi s.l. is difficult and controversial. Previous hypotheses have been based on morphological and anatomical data. We have analyzed sequence variation of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrDNA) among 13 Thlaspi s.l. taxa, representing all sections of the genus. Phylogenetic relationships among ITS sequences of the Thlaspi s.l. taxa studied are in general concordance with a previously published chloroplast DNA based phylogeny of this group. Most-parsimonious trees from ITS and chloroplast DNA data support three groups that are congruent with lineages (Thlaspi s.str., Noccaea–Raparia, Microthlaspi) previously described by Meyer on the basis of seed anatomy. The ITS data grouped Microthlaspi granatense outside the Microthlaspi clade and, therefore, Microthlaspi appeared paraphyletic on the ITS tree, in contrast with the chloroplast DNA phylogeny. We speculate that concerted evolutionary forces have acted among different nrDNA arrays (brought together in M. granatense by hybridization with a related taxon), resulting in the fixation of the alien species nrDNA type in M. granatense, which, however, maintains a Microthlaspi chloroplast genome type. Both molecular data sets detected intraspecific variation among Microthlaspi perfoliatum accessions of different geographic origin and different ploidy levels. Our molecular evidence would suggest the hybrid origin of polyploid M. perfoliatum from diploid M. perfoliatum and M. natolicum. Key words: chloroplast DNA, restriction site variation, sequence analysis of nuclear ribosomal DNA ITS regions, Thlaspi s.l. (Brassicaceae), molecular phylogeny, congruence.

scholarly journals Phenotypic variability confirmed by nuclear ribosomal DNA suggests a possible natural hybrid zone of Triatoma brasiliensis species complex

2016 ◽  
Vol 37 ◽  
pp. 77-87 ◽  
Author(s):  
Jane Costa ◽  
Maria Dolores Bargues ◽  
Vanessa Lima Neiva ◽  
Gena G. Lawrence ◽  
Marcia Gumiel ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Hybrid Zone ◽  
Species Complex ◽  
Phenotypic Variability ◽  
Nuclear Ribosomal Dna ◽  
Natural Hybrid

Molecular phylogeny of Alnus (Betulaceae), inferred from nuclear ribosomal DNA ITS sequences

2003 ◽  
pp. 207-217
Author(s):  
Elisabeth Navarro ◽  
Jean Bousquet ◽  
André Moiroud ◽  
Antonio Munive ◽  
Dominique Piou ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Ribosomal Dna ◽  
Its Sequences ◽  
Nuclear Ribosomal Dna

Molecular phylogeny of the Blastocladiomycota (Fungi) based on nuclear ribosomal DNA

2011 ◽  
Vol 115 (4-5) ◽  
pp. 381-392 ◽  
Author(s):  
Teresita M. Porter ◽  
Wallace Martin ◽  
Timothy Y. James ◽  
Joyce E. Longcore ◽  
Frank H. Gleason ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Ribosomal Dna ◽  
Nuclear Ribosomal Dna
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