Effects of Streptomyces corchorusii, Streptomyces mutabilis, pendimethalin, and metribuzin on the control of bacterial and Fusarium wilt of tomato

1996 ◽  
Vol 74 (7) ◽  
pp. 1016-1022 ◽  
Author(s):  
Abd El-Raheem R. El-Shanshoury ◽  
Soad M. Abu El-Sououd ◽  
Omima A. Awadalla ◽  
Nabila B. El-Bandy
Keyword(s):  
Fusarium Oxysporum ◽  
Culture Media ◽  
Disease Incidence ◽  
Inhibitory Effects ◽  
Pseudomonas Solanacearum ◽  
Negative Effects ◽  
Streptomyces Spp ◽  
Antagonistic Streptomyces

Two Streptomyces spp. and two herbicides were used to control the pathogens of tomato wilt disease in vitro and in vivo. In vitro studies showed inhibitory effects of Streptomyces corchorusii against Fusarium oxysporum f.sp. lycopersici (Sacc.) and inhibitory effects of Streptomyces mutabilis against Pseudomonas solanacearum. In cultures amended with pendimethalin or metribuzin, the growths of P. solanacearum and F. oxysporum were inhibited. The degree of growth inhibition was proportional to the herbicide concentration, with pendimethalin being more effective than metribuzin, and maximum inhibition was at 2.0 × 10−3 M. The growth of S. corchorusii and S. mutabilis was slightly inhibited or enhanced by the herbicides. Supplementation of the herbicides to culture media of the antagonistic Streptomyces spp. increased their inhibitory effects against P. solanacearum and F. oxysporum that were proportional to the herbicide concentrations. Soaking seeds of tomato in the herbicides prior to sowing in sterilized and raw soils and applying S. corchorusii and (or) S. mutabilis to the soils artificially infested with P. solanacearum and (or) F. oxysporum f.sp. lycopersici (Sacc.) 40 days after transplanting revealed significant interactions that gave better control of wilt than either applied alone. The combination of antagonistic Streptomyces spp. was more effective with pendimethalin than with metribuzin and in nonsterilized soil than in sterilized soil. The combination of pendimethalin with S. corchorusii, S. mutabilis, or S. corchorusii plus S. mutabilis was more effective than the single treatment with microbial antagonists or the herbicide against F. oxysporum, P. solanacearum, and Pseudomonas plus Fusarium, respectively. In both soils, the combination of microbial antagonists with pendimethalin was most effective at 2.0 × 10−3 M, disease incidence being reduced to zero and the percent colonization of either pathogen being the lowest. The results also revealed that these combinations minimized the negative effects of the pathogens on tomato growth. This work demonstrates that two compatible control agents, biological and chemical, can be combined to give additional control of a plant pathogen. Keywords: Streptomyces spp., herbicides, Pseudomonas solanacearum, Fusarium oxysporum f.sp. lycopersici (Sacc.), wilt, Lycopersicon esculentum Mill.

Inhibitory effects of UV mutants of Streptomyces corchorusii and Streptomyces spiroverticillatus on bean and banana wilt pathogens

1993 ◽  
Vol 71 (8) ◽  
pp. 1080-1086 ◽  
Author(s):  
Mohamed S. El-Abyad ◽  
Mostafa A. El-Sayed ◽  
Abdel-Reheem El-Shanshoury ◽  
Nadia H. El-Batanouny
Keyword(s):  
Fusarium Wilt ◽  
Uv Irradiation ◽  
Bacterial Wilt ◽  
Disease Incidence ◽  
French Bean ◽  
Inhibitory Effects ◽  
Streptomyces Spp ◽  
Banana Wilt

The purpose of this investigation was to produce improved mutants of Streptomyces corchorusii and Streptomyces spiroverticillatus, using a UV-irradiation regime, which are effective against the causal pathogens of the Fusarium wilt of French bean and the bacterial wilt of banana, respectively. Seven out of the 11 mutants obtained from S. corchorusii were active antagonists against Fusarium oxysporum f.sp. phaseoli; mutant 155 was the most effective. All five mutants obtained from S. spiroverticillatus showed inhibitory effects against Pseudomonas solanacearum; mutant 281 was the most potent. Spore germination, germ-tube elongation, growth, and sporulation of F. oxysporum f.sp. phaseoli were significantly inhibited in the different concentrations of filtrates of either wild or mutant 155 of S. corchorusii in vitro with the mutant being more effective; maximum inhibition was at 80% concentration. The filtrate of either wild or mutant 281 of S. spiroverticillatus sharply decreased the number of colonies of P. solanacearum as its concentration increased up to 80%, at which no growth was obtained. The in vivo utilization of S. corchorusii in the biocontrol of Fusarium wilt of French bean revealed that soaking seeds in filtrate of the antagonistic strain prior to sowing was the most effective treatment and that mutant 155 reduced disease incidence by 83.4% (43.3% for the wild type) compared with the untreated control, in addition to improving plant growth. Key words: antagonism, UV irradiation, mutation, Streptomyces spp., Fusarium wilt, bacterial wilt, French bean, banana.

scholarly journals Self-Inhibitory Activity of Trichoderma Soluble Metabolites and Their Antifungal Effects on Fusarium oxysporum

2020 ◽  
Vol 6 (3) ◽  
pp. 176
Author(s):  
Samuel Álvarez-García ◽  
Sara Mayo-Prieto ◽  
Santiago Gutiérrez ◽  
Pedro Antonio Casquero
Keyword(s):  
Fusarium Oxysporum ◽  
Inhibitory Activity ◽  
Inhibitory Effects ◽  
Trichoderma Spp ◽  
Macroscopic Structure ◽  
Biocontrol Activity ◽  
Microbial Strains ◽  
Phytopathogenic Microorganisms

Self-inhibitory processes are a common feature shared by different organisms. One of the main mechanisms involved in these interactions regarding microorganisms is the release of toxic diffusible substances into the environment. These metabolites can exert both antimicrobial effects against other organisms as well as self-inhibitory ones. The in vitro evaluation of these effects against other organisms has been widely used to identify potential biocontrol agents against phytopathogenic microorganisms. In the present study, we performed membrane assays to compare the self-inhibitory effects of soluble metabolites produced by several Trichoderma isolates and their antifungal activity against a phytopathogenic strain of Fusarium oxysporum. The results demonstrated that Trichoderma spp. present a high self-inhibitory activity in vitro, being affected in both their growth rate and the macroscopic structure of their colonies. These effects were highly similar to those exerted against F. oxysporum in the same conditions, showing no significant differences in most cases. Consequently, membrane assays may not be very informative by themselves to assess putative biocontrol capabilities. Therefore, different methods, or a combination of antifungal and self-inhibitory experiments, could be a better approach to evaluate the potential biocontrol activity of microbial strains in order to pre-select them for further in vivo trials.

The use of natural products against Fusarium oxysporum: A review

2020 ◽  
Vol 10 ◽  
Author(s):  
Zeyneb Belhi ◽  
Noureddine Boulenouar ◽  
Abdelkrim Cheriti
Keyword(s):  
Natural Products ◽  
Fusarium Oxysporum ◽  
Crop Yields ◽  
Environmental Safety ◽  
Negative Effects ◽  
Formae Speciales ◽  
Agricultural Industries ◽  
Different Sources

: Currently, numerous researchers have focused their attention on the use of natural products from plants and microorganisms, or compounds derived from these organisms to protect crops from various diseases, thus reduce the negative effects on human health and environmental safety. Fungal diseases cause a considerable loss of crop yields in agricultural industries worldwide. Fusarium oxysporum comprises a multitude of formae speciales that cause vascular wilt diseases of economically important crops. This review presents an overview of researches realized on natural products tested against Fusarium oxysporum formae speciales phytopathogens for the period (2017-2020). This review aims to collect major research works of the antifungal compounds against these fungi and up-dates information on their developments and approaches that have been rapidly taking place in recent years so that further novel researches can be envisaged. Discussion of these studies by analyzing different sources for antifusariosis treatment, evaluation of testing methods, and information on their advantages and limitations and to determine those with real efficacy. Despite the important number of natural products with remarkable in vitro efficiency, the limiting point is their in vivo application for soil microorganisms (in this case: Fusarium oxysporum). Therefore, more advanced researches are needed to solve this problem.

scholarly journals Serendipita Species Trigger Cultivar-Specific Responses to Fusarium Wilt in Tomato

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 595 ◽  
Author(s):  
Negar Ghezel Sefloo ◽  
Krzysztof Wieczorek ◽  
Siegrid Steinkellner ◽  
Karin Hage-Ahmed
Keyword(s):  
Disease Incidence ◽  
Dry Mass ◽  
In Vitro Assays ◽  
Plant Interactions ◽  
Inhibitory Effects ◽  
Negative Effects ◽  
Beneficial Effects ◽  
Specific Effects ◽  
Tomato Cultivars

The endophytic fungi Serendipita indica and S. vermifera have recently gained increasing attention due to their beneficial effects on plant growth and plant health. Little is known about other species, such as S. williamsii and S. herbamans. To test their biocontrol and growth-promoting potential, susceptible and tolerant tomato cultivars (Kremser Perle and Micro-Tom, respectively) were inoculated with S. williamsii, S. herbamans, S. indica, or S. vermifera and challenged with the soilborne pathogen Fusarium oxysporum f. sp. lycopersici (Fol) in greenhouse experiments. Furthermore, in vitro assays on the direct inhibitory effects of Serendipita spp. against Fol were performed. Negative effects of Fol on phenological growth in the susceptible cultivar were alleviated by all four applied Serendipita spp. Apart from these similar effects on biometric parameters, disease incidence was only reduced by S. herbamans and S. vermifera. In the tolerant cultivar, disease parameters remained unaffected although shoot dry mass was negatively affected by S. vermifera. Direct effects of Serendipita spp. against Fol were not evident in the in vitro assays indicating an indirect effect via the host plant. Our results highlight the importance of identifying cultivar-specific effects in pathogen–endophyte–plant interactions to determine the most beneficial combinations.

scholarly journals Potency of Endophyte Microbes as an Antagonistic Agent for Colletotrichum capsici Causes Anthracnose Disease in Cayenne pepper (Capsicum frustescens)

el–Hayah ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 104-111
Author(s):  
Zahroul Afifah ◽  
Ulfah Utami
Keyword(s):  
Bacillus Cereus ◽  
Culture Media ◽  
Disease Incidence ◽  
Negative Control ◽  
Dual Culture ◽  
Anthracnose Disease ◽  
Colletotrichum Capsici ◽  
Trichoderma Sp

Background: An anthracnose disease caused by pathogenic fungal Colletotrichum capsici has been attacking the cayenne plants either harvested or has not been harvested. This disease must be handled appropriately and quickly because it can reduce the production of chili up to 90%. Recently, anthracnose disease prevention still use chemical fungicide that if applied for long time will cause new impact for environment. Objective: Trichoderma and Bacillus cereus endophytes may be used as antagonistic agents for C. capsici pathogens because they have various antibiotic compounds. Methods: This research uses experimental method. The stages of this study include sterilization of tools and materials, preparation of culture media of fungal and bacteria, rejuvenation of endophytic microbe culture Trichoderma sp. and Bacillus cereus, rejuvenation of  C. capsici pathogen, antagonistic test in vitro using dual culture method. Result and conclusion: The results of in vitro antagonistic tests showed that inhibition percentage of Trichoderma treatment (96%) and combination treatment Trichoderma and B. cereus (97%) is not significantly different. While in B. cereus treatment (11, 88%) significantly different with all of treatments. Endophytes are shown by its dominating growth in petri dishes than C. capsici pathogen or B. cereus endophytes. Furthermore,for endophytes Trichoderma sp continued on in vivo test because it was most effective.The result for incubation period is 3 days after inoculation compared with negative control 2 days. For disease incidence 100%, and for disease intensity that is 61,25% compared with negative control equal to 88,75%.

scholarly journals Uji Efektivitas Ekstrak Kulit Manggis (Garcinia mangostana L.) terhadap Fusarium oxysporum pada Kecambah Kelapa Sawit

2020 ◽  
Vol 8 (1) ◽  
pp. 21
Author(s):  
Tyas Dwi Chintya ◽  
Albertus Sudirman ◽  
Ersan Ersan
Keyword(s):  
Fusarium Oxysporum ◽  
Oil Palm ◽  
Disease Incidence ◽  
Garcinia Mangostana ◽  
Mean Values ◽  
Mangosteen Peel ◽  
In Vivo Testing ◽  
Peel Extract

Fusarium oxysporum is a pathogen that causes wilt in oil palm and can cause oil palm sprout decay. The study aimed to determine the effectivity of mangosteen peel extract (Garcinia mangostana L.) in inhibiting the growth of Fusarium oxysporum in vitro and in vivo. The research was conducted in November 2017 to June 2018 at the Politeknik Negeri Lampung. The method used was a completely randomized design (CRD) consisting of 5 treatments, namely the concentration of mangosteen peel extract 0% (control), 15%, 30%, 45%,and 60%. Data were analyzed using analysis of variance (ANOVA) and further tests of mean values using the LSD test. The results showed that mangosteen peel extract affected the percentage of inhibitory zone extract in Fusarium oxysporum in vitro at concentrations of 15%, 30%, and 45% respectively at 25,92%, 29,06% and 35,95%. The treatment of mangosteen peel extract also affected the percentage of disease incidence and number of leaves in in vivo testing.

Inhibitory effects of epimedium herb water extract on the inflammatory response in vitro and in vivo

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
YC Oh ◽  
YH Jeong ◽  
WK Cho ◽  
SJ Lee ◽  
JY Ma
Keyword(s):  
Inflammatory Response ◽  
Water Extract ◽  
Inhibitory Effects

The Effects of Brinolase (Fibrinolytic Enzyme from Aspergillus Oryzae) on Platelet Aggregation of Dog and Man

1972 ◽  
Vol 28 (01) ◽  
pp. 031-048 ◽  
Author(s):  
W. H. E Roschlau ◽  
R Gage
Keyword(s):  
Platelet Aggregation ◽  
Aspergillus Oryzae ◽  
Degradation Products ◽  
Blood Platelet ◽  
Human Platelets ◽  
Fibrinolytic Enzyme ◽  
Inhibitory Effects ◽  
Blood Platelet Aggregation

SummaryInhibition of blood platelet aggregation by brinolase (fibrinolytic enzyme from Aspergillus oryzae) has been demonstrated with human platelets in vitro and with dog platelets in vivo and in vitro, using both ADP and collagen as aggregating stimuli. It is suggested that the optimal inhibitory effects of brinolase occur indirectly through the generation of plasma fibrinogen degradation products, without compromising platelet viability, rather than by direct proteolysis of platelet structures.

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