Réponse des vignes champenoises aux températures negatives: effet d'un refroidissement contrôlé sur les réserves glucidiques du complexe gemmaire avant et au cours du débourrement

1996 ◽  
Vol 74 (3) ◽  
pp. 492-505 ◽  
Author(s):  
E. Aït Barka ◽  
J. C. Audran

Sugar concentrations were analyzed in grapevine (Vitis vinifera) buds at three different phenological stages (stage 1, dormant bud; stage 2, woolly bud; stage 5, green tip) and were submitted, under controlled conditions, to negative temperatures corresponding to the intracellular liquid crystallization points. In absence of any treatment, starch concentration diminished according to the phenological evolution, indicating its utilization during bud breaking. Starch and raffinose accumulated when controlled cooling was applied, except for stage 1 buds collected on March 6, 1993. Similarly, saccharose concentrations were reduced in bud complexes after temperature reductions. Fructose and glucose concentrations seemed to be less sensitive to thermal variations. The accumulation of starch observed at the end of a cold treatment could be partly explained by the inhibition of amylase activity under cold conditions. Keywords: bud, Vitis vinifera L., phenological stage, thermal stress, sugars. [Journal translation]

1995 ◽  
Vol 73 (12) ◽  
pp. 1878-1888 ◽  
Author(s):  
E. Aït Barka ◽  
J. C. Audran ◽  
O. Brun ◽  
C. Leddet ◽  
J. Dereuddre

Evolution of the exothermic profile of latent buds of Vitis vinifera L. cv. Pinot noir was followed using differential scanning microcalorimetry. Analyses performed on buds at the winterized state (stage 1), on wolly buds (stage 3), and on green pointing buds (stage 5) showed that modes and speed of extracellular and intracellular liquid crystallisation vary according to the phenological stage and are closely linked to the meristematic tissues. Among these, the vascular system plays a major role in the crystallisation process. Indeed, within the winterized buds, where it is at the procambial stage, i.e. nonfunctional, it forms a barrier to the propagation of ice toward the apical part of the bud where water remains in a prolongated surfusion state. On the opposite, during the period preceeding bud opening, from stage 3 to stage 5, where its differentiation leads to a continuous and functional vascular system connecting the bud base to its apical part, it forms a preferred path allowing rapid ice propagation, leading to a violent ice setting in all the tissues. Key words: latent bud, exotherm, calorimetry, vascular system, wine. [Journal translation]


1996 ◽  
Vol 74 (3) ◽  
pp. 413-418 ◽  
Author(s):  
E. Aït Barka ◽  
J. C. Audran

Specific conductivity is often used to determine plant freezing tolerance after a cold treatment. This study was undertaken to determine if there is a correlation between the evolution of aerial organs specific conductivity in vine (Vitis vinifera L.) and the extent of their viability with regard to the applied treatment. The data show two types of increases: the first one was observed along with the phenological evolution, in absence of any treatment, whereas the second appeared after the treatment on the organs. This increment was stronger at the bud than at the shoot level. Electrolyte leakage from the frost treated organs seems to be linked with their sensitivity to cold or the intensity of the applied treatment. Keywords: electrical conductivity, phenological stage, thermal stress, 2,3,5-triphenyltetrazolium chloride, Vitis vinifera L. [Journal translation]


HortScience ◽  
2009 ◽  
Vol 44 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Wanmei Jin ◽  
Jing Dong ◽  
Yuanlei Hu ◽  
Zhongping Lin ◽  
Xuefeng Xu ◽  
...  

Dehydration response element binding (DREB)1b is a cold-inducible transcription factor in Arabidopsis thaliana. DREB1b driven by cauliflower mosaic virus 35S promoter was genetically introduced into grape Vitis vinifera L. cv. Centennial Seedless through Agrobacterium-mediated transformation for improving its cold resistance and exploring new genetic breeding approaches to obtain cold-resistant cultivars. In this study, Southern blot analysis showed the DREB1b gene was integrated into the transgenic grapevines with one to two copies. Northern blot analysis showed the presence of DREB1b transcripts in the independent transgenic lines 3, 5, 6, and 7. Further characterization of transgenic grapevines confirmed that both electrolyte leakage conductivity and the freezing point of the transgenic plants were lower than those of wild-type plants. After the cold treatment at –4 °C for 12 h, 26% of transgenic plants wilted among which 95% plants recovered once being placed under the condition of temperature 22 to 25 °C. However, subjected to the same treatment, 98% of nontransgenic plants wilted and only 2% recovered. Our results lead to the conclusion that activity of DREB1b in the transgenic grape could significantly improve its resistance to cold stress.


1995 ◽  
Vol 13 (1) ◽  
pp. 11-14
Author(s):  
J. Roger Harris ◽  
Nina L. Bassuk

Abstract A single drought episode was applied to two groups of container-grown Corylus colurna L. (Turkish hazelnut) seedlings which had concomitantly reached distinct phenological stages; 1) buds opening and no new root growth visible and 2) shoot extension well underway and new root growth just beginning. Two days after rewetting, root hydraulic conductivity was lower for plants exposed to drought, but there was no phenological stage effect. No differences in root hydraulic conductivity were apparent among well-watered plants of stage 1, 2 and a third stage, 3) shoot extension complete (buds set) and root growth well underway. Twenty five days after return to daily irrigation, those plants subjected to the drying treatment had smaller diameter trunks, but total plant height and dry weight of root-balls were similar. No differences in photosynthetic rate or stomatal conductance were evident 25 days after transplanting.


2020 ◽  
Vol 262 ◽  
pp. 109065 ◽  
Author(s):  
Maria Teresa Prats-Llinàs ◽  
Héctor Nieto ◽  
Theodore M. DeJong ◽  
Joan Girona ◽  
Jordi Marsal

2016 ◽  
Vol 68 (1) ◽  
pp. 60-72 ◽  
Author(s):  
Diana Zapata ◽  
Melba Salazar-Gutierrez ◽  
Bernardo Chaves ◽  
Markus Keller ◽  
Gerrit Hoogenboom

Data in Brief ◽  
2019 ◽  
Vol 27 ◽  
pp. 104595 ◽  
Author(s):  
Jiaqiang Luo ◽  
Jessica Brotchie ◽  
Meng Pang ◽  
Philip John Marriott ◽  
Kate Howell ◽  
...  

2015 ◽  
Vol 13 (3) ◽  
pp. e0801 ◽  
Author(s):  
Natalia Dominguez ◽  
Enrique García-Escudero ◽  
Izaskun Romero ◽  
Ana Benito ◽  
Ignacio Martín

<p>An adequate nutritional state of a crop can be kept by means of a well-designed fertilization plan based on the assessment of the nutrient availability throughout the growing season. The objective of this study was to determine the reliability of leaf blade and petiole diagnosis and the period of validity of their references at both flowering and veraison by means of systematic monitoring throughout the complete growing season. The study was carried out in two plots planted with <em>Vitis vinifera</em> L. cv. Graciano within the AOC Rioja (Spain). Blades and petioles were collected throughout a growing season (2006) and total N, P, K, Ca, Mg, Fe, Mn, Zn, Cu and B concentrations were analyzed in both tissues. Results suggest, in general, that petioles have higher variability and lower analysis reproducibility than blades. Therefore, blade could be a more appropriate tissue to evaluate N, P, K, Ca, and Mg at both flowering and veraison in this variety. Micronutrients in blade and petiole showed different variability behaviour in each of the vineyards studied, therefore, based on our results, it was difficult to determine which one could be the best tissue for the nutritional diagnosis of the ‘Graciano’ variety. Seasonal changes of nutrient concentration in both tissues also confirmed the need for reference values for each tissue and each phenological stage.</p><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>21</w:HyphenationZone> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if !mso]><object classid="clsid:38481807-CA0E-42D2-BF39-B33AF135CC4D" id=ieooui></object> <style> st1\:*{behavior:url(#ieooui) } </style> <![endif]--><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman";} </style> <![endif]-->


Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
H Amira-Guebailia ◽  
T Richard ◽  
S Rouaiguia ◽  
P Waffo Tueguo ◽  
JC Delaunay ◽  
...  

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