Comparative analysis of the in vivo and in vitro metabolites produced by the entomopathogen Beauveria bassiana

1995 ◽  
Vol 73 (S1) ◽  
pp. 1092-1099 ◽  
Author(s):  
D. G. Boucias ◽  
I. Mazet ◽  
J. Pendland ◽  
S. Y. Hung
Keyword(s):  
Beauveria Bassiana ◽  
Biologically Active ◽  
Fungal Metabolites ◽  
Active Metabolites ◽  
Western Blots ◽  
Insect Mycopathogen ◽  
Biologically Active Metabolites ◽  
Germ Tubes

Beauveria bassiana, like other insect mycopathogens, has evolved mechanisms to penetrate the insect exoskeleton via germ tubes and to replicate in the host hemocoel. Our initial studies have shown that biologically active metabolites released in the hemolymph during the vegetative growth phase of B. bassiana disrupt the host immune response and metamorphosis. These components cause an immediate reduction in filopodial-producing hemocytes and an increase in the level of serum phenoloxidase. Radiolabeling of tissues explanted from healthy versus infected larvae has demonstrated both the induction and repression of polypeptides in B. bassiana infected hemolymph. None of the polypeptides detected with 35S pulse labeling were responsible for the cytotoxic and insecticidal activities detected in infected hemolymph. Western blots of SDS gels containing chromatographic fractions from healthy and infected sera probed with both antibodies against B. bassiana cell homogenates and culture filtrates contained a complex of antigens. The results of lectin labeling and sodium periodate treatments suggested that carbohydrates were the major epitopes being recognized by both monoclonal and polyclonal probes. Key words: insect mycopathogen, Beauveria bassiana, fungal metabolites, entomopathogen, Spodoptera exigua.

Metabolomics of Healthy Berry Fruits

2019 ◽  
Vol 25 (37) ◽  
pp. 4888-4902 ◽  
Author(s):  
Gilda D'Urso ◽  
Sonia Piacente ◽  
Cosimo Pizza ◽  
Paola Montoro
Keyword(s):  
Biological Activities ◽  
Biologically Active ◽  
In Vivo Studies ◽  
Bioactive Metabolites ◽  
Active Metabolites ◽  
Positive Effects ◽  
Cell Functions ◽  
Berry Fruits

The consumption of berry-type fruits has become very popular in recent years because of their positive effects on human health. Berries are in fact widely known for their health-promoting benefits, including prevention of chronic disease, cardiovascular disease and cancer. Berries are a rich source of bioactive metabolites, such as vitamins, minerals, and phenolic compounds, mainly anthocyanins. Numerous in vitro and in vivo studies recognized the health effects of berries and their function as bioactive modulators of various cell functions associated with oxidative stress. Plants have one of the largest metabolome databases, with over 1200 papers on plant metabolomics published only in the last decade. Mass spectrometry (MS) and NMR (Nuclear Magnetic Resonance) are the most important analytical technologies on which the emerging ''omics'' approaches are based. They may provide detection and quantization of thousands of biologically active metabolites from a tissue, working in a ''global'' or ''targeted'' manner, down to ultra-trace levels. In the present review, we highlighted the use of MS and NMR-based strategies and Multivariate Data Analysis for the valorization of berries known for their biological activities, important as food and often used in the preparation of nutraceutical formulations.

The chemistry of fungal antagonism and defense

1995 ◽  
Vol 73 (S1) ◽  
pp. 1265-1274 ◽  
Author(s):  
James B. Gloer
Keyword(s):  
Interdisciplinary Studies ◽  
Biologically Active ◽  
Model Systems ◽  
Fungal Metabolites ◽  
Ecological Studies ◽  
Active Metabolites ◽  
Broad Array ◽  
Fungal Antagonism ◽  
Antifungal Chemotherapy ◽  
Biologically Active Metabolites

Mechanisms of fungal antagonism and defense often include the production of biologically active metabolites by one species that exert effects on potential competitors and (or) predators. Studies carried out in our laboratory and others clearly indicate that such ecological phenomena can serve as valuable leads to the discovery of novel and potentially useful bioactive fungal metabolites. There is evidence that some of these compounds may render advantages to the producing organism, although careful and definitive ecological studies are required to determine this. Nevertheless, the results summarized here demonstrate the broad array of possible benefits that can arise from interdisciplinary studies in this area. This paper focuses primarily on our own investigations of the chemistry involved in fungal antagonism and defense using coprophilous and sclerotial fungi as model systems. These results have potential implications in many areas of study, including fungal ecology, secondary metabolism, chemotaxonomy, organic chemistry, structure determination, antifungal chemotherapy, and insect control. Key words: fungi, antifungal, insecticide, antagonism, chemical defense, secondary metabolites.

scholarly journals Composition of Intracellular and Cell Wall-Bound Phlorotannin Fractions in Fucoid Algae Indicates Specific Functions of These Metabolites Dependent on the Chemical Structure

Metabolites ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 369
Author(s):  
Claudia Birkemeyer ◽  
Valeriya Lemesheva ◽  
Susan Billig ◽  
Elena Tarakhovskaya
Keyword(s):  
Cell Wall ◽  
Algal Species ◽  
Biologically Active ◽  
Active Metabolites ◽  
Subcellular Compartments ◽  
Plant Biochemistry ◽  
New Findings ◽  
Fucoid Algae ◽  
Biologically Active Metabolites

Accumulation of biologically active metabolites is a specific feature of plant biochemistry, directing the use of plants in numerous applications in the pharmaceutical and food industries. Among these substances, the plethora of phenolic compounds has attracted particular interest among researchers. Here, we report on new findings in phlorotannin research, a large group of multifunctional phenolic substances, produced in brown algae. Comprehensive LC-MS profiling of three algal species allowed us to depict the complex pattern of this structurally diverse compound group across different tissues and subcellular compartments. We compiled more than 30 different phlorotannin series in one sample and used accurate mass spectrometry to assign tentative structures to the observed ions based on the confirmed sum formulas. From that, we found that acetylation, hydroxylation, and oxidation are likely to be the most common in vivo modifications to phlorotannins. Using an alternative data mining strategy to cope with extensive coelution and structural isomers, we quantitatively compared the intensity of different phlorotannin series in species, tissues, and subcellular compartments to learn more about their physiological functions. The structure and intra-thallus profiles of cell wall-bound phlorotannins were studied here for the first time. We suggest that one of the major dibenzodioxin-type phlorotannin series may exclusively target integration into the cell wall of fucoid algae.

Avidin expression during chick chondrocyte and myoblast development in vitro and in vivo: regulation of cell proliferation

2001 ◽  
Vol 114 (8) ◽  
pp. 1473-1482 ◽  
Author(s):  
B. Zerega ◽  
L. Camardella ◽  
S. Cermelli ◽  
R. Sala ◽  
R. Cancedda ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Differentiation ◽  
Fatty Acid Biosynthesis ◽  
Culture Conditions ◽  
Biologically Active ◽  
Western Blots ◽  
Inflammatory Stimuli ◽  
Development In Vitro

Avidin is a major [(35)S]methionine-labeled protein induced by bacterial lipopolysaccharide (LPS) and interleukin 6 (IL-6) in cultured chick embryo myoblasts and chondrocytes. It was identified by N-terminal sequencing of the protein purified from conditioned culture medium of LPS-stimulated myoblasts. In addition, avidin was secreted by unstimulated myoblasts and chondrocytes during in vitro differentiation; maximal expression being observed in differentiated myofibers and hypertrophic chondrocytes. In developing chick embryos, immunohistochemistry revealed avidin in skeletal muscles and growth plate hypertrophic cartilage. Avidin was secreted into culture as a biologically active tetramer. Exogenous avidin added to the medium of proliferating chondrocytes progressively inhibited cell proliferation, whereas addition of avidin to differentiating chondrocytes in suspension allowed full cell differentiation. No toxic effects for the cells were observed in both culture conditions. Western blots of samples from cytosolic extracts using alkaline-phosphatase-conjugated streptavidin showed three biotin-containing proteins. Acetyl-CoA carboxylase was identified by specific antibodies. Based on these data, we propose that avidin binds extracellular biotin and regulates cell proliferation by interfering with fatty acid biosynthesis during terminal cell differentiation and/or in response to inflammatory stimuli.

scholarly journals Biomedical Properties of Edible Seaweed in Cancer Therapy and Chemoprevention Trials: A Review

2013 ◽  
Vol 8 (12) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Farideh Namvar ◽  
Paridah Md. Tahir ◽  
Rosfarizan Mohamad ◽  
Mahnaz Mahdavi ◽  
Parvin Abedi ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Biologically Active ◽  
Chemical Components ◽  
Therapeutic Effects ◽  
Active Metabolites ◽  
Edible Seaweed ◽  
Anticancer Properties ◽  
In Vivo Experiments

This review article summarizes in vitro and in vivo experiments on seaweed anticancer activity and seaweed chemical components. Seaweed use in cancer therapy, chemopreventive randomized control trials (RCTs) and quasi-experiments are discussed. The literature reviewed in this article was obtained from various scientific sources and encompasses publications from 2000–2012. Seaweed therapeutic effects were deemed scientifically plausible and may be partially explained by the in vivo and in vitro pharmacological studies described. Although the mechanisms of action remain unclear, seaweed's anticancer properties may be attributable to its major biologically active metabolites. Much of the seaweed research outlined in this paper can serve as a foundation for explaining seaweed anticancer bioactivity. This review will open doors for developing strategies to treat malignancies using seaweed natural products.

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

Spices with Breast Cancer Chemopreventive and Therapeutic Potentials: A Functional Foods Based-Review

2018 ◽  
Vol 18 (2) ◽  
pp. 182-194 ◽  
Author(s):  
Aliyu Muhammad ◽  
Mohammed Auwal Ibrahim ◽  
Ochuko Lucky Erukainure ◽  
Ibrahim Malami ◽  
Auwal Adamu
Keyword(s):  
Breast Cancer ◽  
Urban Areas ◽  
Cancer Chemoprevention ◽  
Biologically Active ◽  
Active Metabolites ◽  
Rural And Urban Areas ◽  
Anticancer Properties ◽  
Rural And Urban ◽  
Breast Cancer Chemoprevention ◽  
Biologically Active Metabolites

Background: Cancer is a multifaceted metabolic disease that affects sizeable dwellers of rural and urban areas. Among the various types of cancer, mammary cancer is one of the most frequently diagnosed cancers in women. Its menace can be curbed with locally consumed spices due to their multiple bioactive phytochemicals. Aims: This review focuses on the breast cancer chemopreventive and therapeutic potentials of locally consumed spices. Methods/Results: The most commonly consumed spices with breast cancer chemopreventive and chemotherapeutic phytochemical include pepper, onions, ginger, garlic, curry and thyme containing many biologically active metabolites ranging from vitamins, fatty acids esters, polyphenols/phenolics, sulfurcontaining compounds and anthraquinones with proven antioxidant, anti-inflammatory, immuno-modulatory, antitumor and anticancer properties against breast cancer/carcinogenesis. Therefore, extracts and active principles of these spices could be explored in breast cancer chemoprevention and possibly therapeutically which may provide an avenue for reducing the risk and prevalence of breast cancer.

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

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