scholarly journals Nuclear DNA content in the subgenus Coffea (Rubiaceae): inter- and intra-specific variation in African species

1995 ◽  
Vol 73 (1) ◽  
pp. 14-20 ◽  
Author(s):  
J. Cros ◽  
M. C. Combes ◽  
N. Chabrillange ◽  
S. Hamon ◽  
C. Duperray ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Interspecific Hybrids ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
African Species ◽  
Intermediate Group ◽  
Coffea Species ◽  
Specific Variation ◽  
Angiosperm Species

Flow cytometry has been used to estimate nuclear DNA content of 13 Coffea species (Rubiaceae) native to Africa. Twelve diploid (2n = 22) and one tetraploid (C. arabica, 2n = 44) species were investigated. Isolated nuclei from 77 genotypes were stained with propidium iodide (PI; not base specific). Thirty-nine genotypes were stained with 4′,6-diamidino-2-phenylindole (DAPI; AT specific). Nuclear DNA content (2C values), estimated with PI, ranged from 0.95 to 1.78 pg. By aggregative clustering, three groups of accessions with increasing DNA content were identified. Three species, namely C. sessiliflora, C. racemosa, and C. pseudozanguebariae, had a low DNA content (0.90 – 1.30 pg). Three species, namely C. eugenioides, C. stenophylla, and C. sp. F, were exclusively found in the intermediate group (1.31 – 1.60 pg). The remaining species were distributed between the intermediate group and the last group (1.61 – 1.80 pg). The values determined for the Coffea species are compared, inter- and intra-specifically, to those of other angiosperm species. The observed differences are discussed according to the ecogeographic origin of the species, their phenological characteristics, and the fertility of the F1 interspecific hybrids. Key words: Africa, Coffea, flow cytometry, nuclear DNA content, genome evolution.

Analysis of intraspecific nuclear DNA content variation in Gleditsiatriacanthos by flow cytometry

1995 ◽  
Vol 25 (3) ◽  
pp. 440-445
Author(s):  
CM. Wake ◽  
P.R. Schaefer ◽  
L.K. Jost ◽  
D.P. Evenson
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Dna Content ◽  
Blood Cells ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Evolutionary Changes ◽  
Wide Range ◽  
Dna Content Variation ◽  
Angiosperm Species

Honeylocust trees (Gleditsiatriacanthos L.) are distributed across a wide range of climatic and edaphic conditions, demonstrating considerable variation in cold, drought, and salinity tolerances. In many angiosperm species, an evolutionary change in nuclear DNA content accompanies colonization into more stressful environments. Fresh leaf nuclei were isolated from 22 honeylocust sources from across the species' native range, mixed with trout red blood cells, stained with propidium iodide, and their DNA content was measured by flow cytometry. According to DNA stainability of the trout red blood cells, leaf nuclei had a DNA content of 1.72 ± 0.02 pg (mean ± SD). Variation in DNA content among the sources was not significant (p < 0.05), suggesting that adaptive evolutionary changes in honeylocust are not evident in increasing or decreasing amounts of leaf nuclear DNA content.

Estimation of nuclear DNA content by flow cytometry in fishes of the genus Xiphophorus

1989 ◽  
Vol 94 (3) ◽  
pp. 465-468 ◽  
Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

scholarly journals Brief communication. First evaluation of nuclear DNA content in Setaria genus by flow cytometry

1998 ◽  
Vol 89 (6) ◽  
pp. 556-559 ◽  
Author(s):  
M Le Thierry d'Ennequin ◽  
O Panaud ◽  
S Brown ◽  
S Siljak-Yakovlev ◽  
A Sarr
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

Identification of chromosomal deficiency by flow cytometry and cytogenetics in mutant tomato (Solanum lycopersicum, Solanaceae) plants

2009 ◽  
Vol 57 (5) ◽  
pp. 444 ◽  
Author(s):  
Isane Vera Karsburg ◽  
Carlos Roberto Carvalho ◽  
Wellington Ronildo Clarindo
Keyword(s):  
Flow Cytometry ◽  
Solanum Lycopersicum ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Chromosome 1 ◽  
Wild Type ◽  
Extrinsic Factors ◽  
Solanum Lycopersicum L ◽  
Morphogenic Process

Structural chromosomal aberrations can occur spontaneously in plant karyotypes as a result of both intrinsic and extrinsic factors. These aberrations may affect sporophyte fitness because fundamental genes involved with distinct morphogenic process may be lost. Inadequate development of flowers and anomalous fruits without seeds has been observed in plants of Solanum lycopersicum L. (Solanaceae) ‘BHG 160’ of the tomato germplasm bank (Universidade Federal de Viçosa, Brazil). The nuclear DNA content, quantified by flow cytometry, showed that mutant ‘BHG 160’ possesses 0.09 pg (4.59%) less nuclear DNA content than does the wild-type ‘BGH 160’. Improved cytogenetical preparations evidenced that this difference was due to a spontaneous terminal deficiency in the short arm of the mutant ‘BGH 160’ Chromosome 1. These results suggest that the genes encoded in the short arm of Chromosome 1 may be involved in the development of flowers and fruits in the tomato.

Analysis of Nuclear DNA content in plant cells by Flow cytometry

1989 ◽  
Vol 31 (2) ◽  
pp. 113-120 ◽  
Author(s):  
J. Dpooležel ◽  
Pavla Binarová ◽  
S. Lcretti
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Plant Cells ◽  
Nuclear Dna Content

scholarly journals Isolates of Verticillium dahliae Pathogenic to Crucifers Are of at Least Three Distinct Molecular Types

2003 ◽  
Vol 93 (3) ◽  
pp. 364-376 ◽  
Author(s):  
Alex Collins ◽  
C. Ada N. Okoli ◽  
Anne Morton ◽  
David Parry ◽  
Simon G. Edwards ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Verticillium Dahliae ◽  
Fragment Length ◽  
Dna Content ◽  
Interspecific Hybrids ◽  
Nuclear Dna ◽  
Fragment Length Polymorphism ◽  
Nuclear Dna Content ◽  
Molecular Evidence ◽  
Aflp Analysis

Diverse isolates of the soilborne wilt fungi Verticillium dahliae and V. albo-atrum were studied to understand the nature and origins of those infecting cruciferous hosts. All isolates from cruciferous crops produced microsclerotia, and the majority produced long conidia with a high nuclear DNA content; these isolates were divided into two groups by amplified fragment length polymorphism (AFLP) analysis. One group could be subdivided by other criteria such as rRNA sequences and mitochondrial DNA restriction fragment length polymorphism (RFLP) analysis. Two crucifer isolates were short spored and had a low nuclear DNA content. The results are consistent with the crucifer isolates being interspecific hybrids. The long-spored isolates are best regarded as amphihaploids (or allodiploids) with the AFLP groups probably each representing separate interspecific hybridization events. The short-spored crucifer isolates appear to be derived from interspecific hybrids and are here called ‘secondary haploids’. Molecular evidence suggests that one parent in the crosses was similar to V. dahliae. The other parent of the amphihaploids seems to have been more similar to V. albo-atrum than to V. dahliae, but was distinct from all isolates of either species so far studied. The implications for the taxonomy of crucifer isolates are discussed and the use of the name V. longisporum, proposed elsewhere for just some of these isolates, is discouraged.

Estimation of nuclear DNA content in plants using flow cytometry

2007 ◽  
Vol 2 (9) ◽  
pp. 2233-2244 ◽  
Author(s):  
Jaroslav Doležel ◽  
Johann Greilhuber ◽  
Jan Suda
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content
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