Influence de la qualité de l'éclairement sur les corrélations entre le cotylédon et son bourgeon axillaire chez le Linum usitatissimum

1992 ◽  
Vol 70 (5) ◽  
pp. 1087-1092
Author(s):  
Ahmed Fathi Oueida ◽  
Marie-Odile Desbiez

The precedence of one cotyledonary bud over another, as a result of the removal of one of the cotyledons, can be measured quantitatively using the precedence index, g. When g tends towards +1, it is the bud opposite to the deleted cotyledon that takes the precedence, whereas when it tends toward −1, it is the axillant one. When g is close to 0, it corresponds to a globally symmetrical population of plants, in which the bud opposite to the deleted cotyledon and the axillant bud take the precedence in an approximately equal number of plants. When the flax is taken, after decapitation and removal of one cotyledon, on deficient nutrient solution under photoperiodicity of 9 h light: 15 h dark, index g is not affected if the dark period is replaced totally by a low-energy (3 W∙m−2) red light or partly by four periods of 20 min of red light during the dark period. In contrast, blue or far red light, under the same experimental conditions, caused g to tend towards 0 for all cultivars. This effect of the blue or far red light was antagonized by a following red light treatment, which suggests the interference of phytochrome. These observations were not observed with a Knop, ion-rich nutrient solution and at 18 W∙m−2, where g index remained close to +1. Key words: Linum usitatissimum, photomorphogenesis, bud, cotyledon, correlation.

1972 ◽  
Vol 50 (1) ◽  
pp. 1-7 ◽  
Author(s):  
W. G. Hopkins

Control over elongation in Avena coleoptile and Pisum epicotyl segments by cyclic far-red light treatment is dependent on the timing of successive irradiations and independent of total energy. The effect of each far-red irradiation in a cycle is cumulative. Maximum efficiency of far-red light in a cycle is obtained when the light treatments follow within 2 to 3 h of each other. Additional increments of elongation may also be obtained by successive red light treatment only when presented at least 8 to 9 h apart. The effect of a single far-red treatment is to establish a maximum rate of elongation which is maintained for no more than 3 h. The results offer support for the hypothesis that low-energy phytochrome mediated responses result from maintenance of low but maximally effective levels of Pfr and that Pfr destruction is essentially independent of physiological action.


2021 ◽  
Vol 22 (15) ◽  
pp. 7879
Author(s):  
Yingxia Gao ◽  
Yi Zheng ◽  
Léon Sanche

The complex physical and chemical reactions between the large number of low-energy (0–30 eV) electrons (LEEs) released by high energy radiation interacting with genetic material can lead to the formation of various DNA lesions such as crosslinks, single strand breaks, base modifications, and cleavage, as well as double strand breaks and other cluster damages. When crosslinks and cluster damages cannot be repaired by the cell, they can cause genetic loss of information, mutations, apoptosis, and promote genomic instability. Through the efforts of many research groups in the past two decades, the study of the interaction between LEEs and DNA under different experimental conditions has unveiled some of the main mechanisms responsible for these damages. In the present review, we focus on experimental investigations in the condensed phase that range from fundamental DNA constituents to oligonucleotides, synthetic duplex DNA, and bacterial (i.e., plasmid) DNA. These targets were irradiated either with LEEs from a monoenergetic-electron or photoelectron source, as sub-monolayer, monolayer, or multilayer films and within clusters or water solutions. Each type of experiment is briefly described, and the observed DNA damages are reported, along with the proposed mechanisms. Defining the role of LEEs within the sequence of events leading to radiobiological lesions contributes to our understanding of the action of radiation on living organisms, over a wide range of initial radiation energies. Applications of the interaction of LEEs with DNA to radiotherapy are briefly summarized.


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1420
Author(s):  
Takahiro Ueda ◽  
Miki Murata ◽  
Ken Yokawa

Environmental light conditions influence the biosynthesis of monoterpenes in the mint plant. Cyclic terpenes, such as menthol, menthone, pulegone, and menthofuran, are major odor components synthesized in mint leaves. However, it is unclear how light for cultivation affects the contents of these compounds. Artificial lighting using light-emitting diodes (LEDs) for plant cultivation has the advantage of preferential wavelength control. Here, we monitored monoterpene contents in hydroponically cultivated Japanese mint leaves under blue, red, or far-red wavelengths of LED light supplements. Volatile cyclic monoterpenes, pulegone, menthone, menthol, and menthofuran were quantified using the head-space solid phase microextraction method. As a result, all light wavelengths promoted the biosynthesis of the compounds. Remarkably, two weeks of blue-light supplement increased all compounds: pulegone (362% increase compared to the control), menthofuran (285%), menthone (223%), and menthol (389%). Red light slightly promoted pulegone (256%), menthofuran (178%), and menthol (197%). Interestingly, the accumulation of menthone (229%) or menthofuran (339%) was observed with far-red light treatment. The quantification of glandular trichomes density revealed that no increase under light supplement was confirmed. Blue light treatment even suppressed the glandular trichome formation. No promotion of photosynthesis was observed by pulse-amplitude-modulation (PAM) fluorometry. The present result indicates that light supplements directly promoted the biosynthetic pathways of cyclic monoterpenes.


SLEEP ◽  
2021 ◽  
Vol 44 (Supplement_2) ◽  
pp. A287-A288
Author(s):  
Joey W Chan ◽  
Y K Wing ◽  
S P Lam ◽  
Shirley Xin Li ◽  
J Zhang

Abstract Introduction Drop out during treatment hampers therapeutic effect of interventions. The current study examines the possible predictors of drop out during the five-week light treatment in patients with unipolar non-seasonal depression and evening-chronotype. Methods Baseline characteristics including demographics, sleep diary parameters, light treatment prescribed, and early clinical outcomes changes were compared between the Drop out and Non drop out group. Logistic regression analysis was used to examine predictors for drop out. All data were analyzed in a modified intention to treat analysis with last observation carried forward approach. Results A total of 91 subjects (Female 79%, 46.3 ± 11.8 years old) were included in the analysis. There was no significant difference in the baseline demographic and clinical characteristics between the Drop out and Non drop out group. There was also no significant difference in the improvement of clinical parameters over the first week among the two groups. However, treatment non-adherence (in terms of compliance of less than 80% of prescribed duration) over the first treatment week predicts a five-fold increase in risk of drop out during light therapy. (OR: 5.85, CI: 1.414–24.205, p=0.015) after controlling for potential confounders including age, gender, treatment group, patient expectation, and treatment-emergent adverse events. Conclusion This study found that baseline clinical characteristics including depression severity and improvement of depressive symptoms in the initial week did not differ between the Drop out and Non drop out group. The drop out was also not affected by the type of light (dim red versus bright red light), indirectly supporting dim red light as a valid placebo in bright light therapy trial. Treatment adherence is the early phase of light treatment is an important predictor of drop out. Support (if any):


2012 ◽  
Vol 17 (1) ◽  
pp. 72
Author(s):  
Janeth Arias-Palacios ◽  
Libardo Hernandez-Esquivel ◽  
Juan Carlos Marín-Díaz ◽  
Natalia Navarro-Peña ◽  
Natalia Santos-Arévalo

<strong>Objective</strong>. Evaluate the dilution-neutralization method proposed in the Colombian Technical Norm 5473/07, by using a gel, alcoholbased disinfectant. <strong>Materials and methods</strong>. This study was done using Pseudomonas aeruginosa ATCC 15442, Staphylococcus aureus ATCC 6538, and Enterococcus hirae ATCC 10541 as the assay microorganisms. The study was carried out at 20±1°C as obligatory temperature and additionally at 36±1°C. Four contact times between microorganisms and the disinfectant were evaluated (0, 2, 5 and 10 minutes). The assay was done both under clean conditions (0.3 g/L of bovine serum albumin), and unclean conditions (3 g/L of bovine serum albumin and 3g/L of sheep erythrocytes). <strong>Results</strong>. The implementation of this method produced precise results in all of the six<br />repetitions used during the assay. The obtained results demonstrated a logarithmic reduction higher than five, demonstrating the bactericidal activity exerted by the disinfectant on the control microorganisms. The established experimental conditions and methodology did not affect negatively the growth of any of the strains of microorganisms. Similarly, the neutralizing used did not inhibit the development of the microorganisms of the assay.<strong> Conclusions</strong>. The method was verified by means of the fulfillment of the limits set by the rule. Our results suggest that the method evaluated by means of the implementation of the protocol established in the Colombian Technical Norm 5473/07, allows evaluating the effectiveness of a disinfectant under selected and controlled experimental conditions.<br /><br /><strong>Key words</strong>: disinfection, clean conditions, unclean conditions, dilution-neutralization method, logarithmic reduction.


1991 ◽  
Vol 46 (11-12) ◽  
pp. 1038-1044 ◽  
Author(s):  
Michel Havaux ◽  
Murielle Eyletters

Abstract Preillumination of intact pea leaves with a strong blue-green light of 400 W m-2 markedly inhibited both photoacoustically monitored O2-evolution activity and PS II photochemistry as estimated from chlorophyll fluorescence measurements. The aim of the present work was to examine, with the help of the photoacoustic technique, whether this high-light treatment deteriorated the in vivo PS I function too. High-frequency photoacoustic measurements indicated that photochemical conversion of far-red light energy in PS I was preserved (and even transiently stimulated) whereas photochemical energy storage monitored in light exciting both PS I and PS II was markedly diminished. Low-frequency photoacoustic measurements of the Emerson enhancement showed a spectacular change in the PS II/PS I activity balance in favor of PS I. It was also observed that the linear portion of the saturation curve of the far-red light effect in the Emerson enhancement was not changed by the light treatment. Those results lead to the conclusion that, in contrast to PS II, the in vivo PS I photofunctioning was resistant to strong light stress, thus confirming previous suggestions derived from in vitro studies. Estimation of the redox state of the PS I reaction center by leaf absorbance measurements at ca. 820 nm suggested that, under steady illumination, a considerably larger fraction of PS I centers were in the closed state in high-light pretreated leaves as compared to control leaves, presumably allowing passive adjustment of the macroscopic quantum yield of PS I photochemis­ try to the strongly reduced photochemical efficiency of photoinhibited PS II.


Author(s):  
Wenfeng Xu ◽  
Peng Sun ◽  
Guogang Yang

Abstract Sector cascade experiments can not only be convenient to measure various aerodynamic parameters but also reveal the real flow characteristics in turbomachinery. However, the sector cascade is only a part of the whole annular cascade. The circumferential angle, the structure of the side guide plate (SGP) and the suction mode on the SGP have a great effect on the periodicity of the flow field. Therefore, the effect of structure on periodicity must be taken into consideration in order to obtain accurate data of the sector cascade experiment. In this paper, a compressor sector cascade composed of a row of adjustable guide vanes (AGVs) and a row of stators is designed. The effect of the circumferential angle, SGP structure and suction position on the periodicity is studied by numerical simulation. An optimal cascade scheme is selected for experimental research. The results show that a larger circumferential angle can weaken the effect of low-energy fluid near the SGP on the middle passages. However, given the limited experimental conditions, the circumferential angle is set at 110° which consists of 15 AGVs and 14 stators. What’s more, the SGP with the same bowed angle of AGV on both sides of the cascade can reduce the influence of the SGP on the adjacent passages and obtain a regular periodicity. The low-energy fluids still accumulate near the SGP. The suction near the stator suction side of the SGP can alleviate the blockage in the flow passage and further improve the periodicity of the cascade. Serious analysis of the experiment results have further identified that the suction near the stator suction side of SGP can make the aerodynamic parameters of the flow field uniform and lead to a good periodicity. At the same time, the feasibility of this design method is verified.


Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1475 ◽  
Author(s):  
Yamin Li ◽  
Rui Shi ◽  
Haozhao Jiang ◽  
Linyuan Wu ◽  
Yiting Zhang ◽  
...  

Four light treatments (W: white light; EOD-B: end-of-day enhanced blue light; EOD-FR: end-of-day supplementary far-red light; EOD-UV: end-of-day supplementary ultraviolet-A light) were designed to explore the effects of end-of-day (EOD) lightings (30 min before dark period) on leaf color, biomass and phytochemicals accumulation in two lettuce cultivars (Lactuca sativa cv. ‘Red butter’ and ‘Green butter’) in artificial light plant factory. EOD-FR stimulated the plant and shoot biomass of two cultivars, and EOD-B suppressed the growth of ‘Red butter’ but induced higher biomass in ‘Green butter’. EOD lightings generated brighter, greener and yellower leaf in ‘Red butter’ at harvest, but the highest lightness and the deepest redness of ‘Green butter’ leaf were observed in the middle growth stage. ‘Red butter’ had prominent higher contents of chlorophylls and carotenoids, while these pigments showed less sensitivity to the interaction of cultivars and EOD lightings. EOD lightings impeded the accumulation of anthocyanin in two cultivars, except EOD-UV slightly increased the anthocyanin contents in ‘Green butter’. EOD-UV strengthened the antioxidant capability of ‘Green butter’, but EOD lightings had different effects on the antioxidant and nutritional compound contents in two lettuce cultivars.


1960 ◽  
Vol 15 (4) ◽  
pp. 205-213 ◽  
Author(s):  
Erwin Bünning ◽  
Gabriele Joerrens

In Pieris brassicae, diapause is inhibited if long-day conditions are imposed during and immediately after the third molting. The critical daylength is approximately 14 hours. Under short-day conditions with a main light period of 6 or 12 hours’ duration, supplementary light given in the period from 14 to 16 hours after the beginning of the main light period will inhibit diapause. In contrast to this effect of late exposures to light, light given from 1 to 12 hours after the beginning of the main light period promotes diapause. Experiments with extremely long light periods (10—35 hours), but always with a dark period of 10 hours, show that these diurnal fluctuations in quantitative and qualitative responses to light can continue endogenously for several days. Thus, this time-measuring process operates through the mechanism of endogenous diurnal oscillations in just the same way as do photoperiodic reactions in plants.The inhibition of diapause by light in the second half of the diurnal oscillation (under long days or by light interruptions in the dark period) and the promotion by light in the first half (under short days) occur only with light of short wavelengths: ultraviolet, violet, and blue up to about 550 mμ. Yellow and red light act in the opposite fashion, giving diapause inhibition in the first half of the cycle and promotion in the second half. In white light the violet reaction predominates, so that diapause is promoted by short days and inhibited by long days.


1975 ◽  
Vol 30 (1-2) ◽  
pp. 67-68 ◽  
Author(s):  
Hans K. Kleudgen ◽  
Hartmut K. Lichtenthaler

Abstract Short time red pulses, given 6 times for 5 min within 36 h, induce in etiolated barley seedlings an enhanced synthesis of the main chloroplast carotenoids β-carotene, violaxanthine, lutein and neoxanthine. The level of antheraxanthine and zeaxanthine decreases by red light treatment. These red light effects are reverted by subsequent short time far-red pulses. The results show that the white light induced change in the accumulation rate of individual carotenoids is initiated and regulated by active phytochrome Pfr . In the case of neoxanthin and zeaxanthin the red light effects cannot be fully reverted by far-red; this points to very fast phytochrome reaction.


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