Detection of a subfamily of genes within the soybean nodulin-A multigene family

1991 ◽  
Vol 69 (12) ◽  
pp. 2663-2669 ◽  
Author(s):  
Sylvia G. Gottlob-McHugh ◽  
Douglas A. Johnson
Keyword(s):  
Sequence Analysis ◽  
Key Words ◽  
Gene Conversion ◽  
Cdna Clone ◽  
Multigene Family ◽  
Bradyrhizobium Japonicum ◽  
Root Nodules ◽  
Specific Gene ◽  
Oligonucleotide Probes ◽  
Related Gene

A soybean cDNA clone, 15-9-A, corresponding to a new nodule-specific gene was isolated. 15-9-A corresponds to an abundantly transcribed 1.0-kb mRNA that could first be detected in root nodules at about 10 days following inoculation with Bradyrhizobium japonicum. This clone was found to be very closely related to, but distinct from, Ngm-20, a member of a soybean nodulin multigene family. We have used oligonucleotide probes to detect an additional closely related gene, Ngm-20r, which is expressed as a 0.8-kb mRNA. 15-9-A, Ngm-20, and Ngm-20r appear to form a subfamily within the larger nodulin multigene family. Results derived from sequence analysis and hybridization experiments suggest that gene conversion may have played a role in the evolution of this subfamily. Key words: cDNA clone, gene conversion, multigene family, nodule specific, soybean.

scholarly journals Isolation and sequence analysis of a cDNA clone encoding the fifth complement component.

1985 ◽  
Vol 260 (4) ◽  
pp. 2108-2112
Author(s):  
A B Lundwall ◽  
R A Wetsel ◽  
T Kristensen ◽  
A S Whitehead ◽  
D E Woods ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Cdna Clone ◽  
Complement Component

Strain distribution and in planta production of an extracellular polysaccharide depolymerase from Bradyrhizobium japonicum

1992 ◽  
Vol 38 (8) ◽  
pp. 857-861 ◽  
Author(s):  
Michael F. Dunn ◽  
Arthur L. Karr
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Root Nodules ◽  
Extracellular Polysaccharide ◽  
Extracellular Polysaccharides ◽  
Neutral Sugar ◽  
In Planta ◽  
In Vitro Production ◽  
Polysaccharide Composition ◽  
Vitro Production

Thirty-four strains of Bradyrhizobium japonicum were screened for the in vitro production of an extracellular polysaccharide depolymerase active against the B. japonicum acidic extracellular polysaccharide that contains mannose, glucose, galactose, and 4-O-methylgalactose as neutral sugar components. Over 90% of tested strains producing this type of extracellular polysaccharide also produced the extracellular polysaccharide depolymerase, whereas strains producing a compositionally different extracellular polysaccharide did not. In addition, representatives of species related to B. japonicum by extracellular polysaccharide composition or host range were also phenotypically depolymerase negative. Depolymerase was also present in soybean root nodules formed by B. japonicum strain 2143. In contrast to the cell-associated depolymerase activity found in free-living cells of this strain, most of the depolymerase activity present in nodules is free of the bacteroids. The widespread occurrence of the depolymerase among B. japonicum strains and the spatiotemporal distribution of its activity in planta are consistent with the enzyme playing a role in the removal of surface extracellular polysaccharide from the microorganism during the infection of nodulation process. Key words: Bradyrhizobium japonicum, soybean, extracellular polysaccharides, extracellular polysaccharide depolymerase, bacteroids.

Stage-specific expression of a developmentally regulated gene in Dirofilaria immitis

1992 ◽  
Vol 66 (1) ◽  
pp. 62-67 ◽  
Author(s):  
S. Sun ◽  
T. Matsuura ◽  
K. Sugane
Keyword(s):  
Gene Expression ◽  
Cdna Clone ◽  
Dirofilaria Immitis ◽  
Mrna Level ◽  
Specific Antigen ◽  
Specific Gene ◽  
Developmentally Regulated ◽  
Specific Expression ◽  
Specific Gene Expression

ABSTRACTA previously reported cDNA clone encoding 34 kDa antigenic polypeptide of Dirofilaria immitis (λ cD34) was studied to elucidate the mechanism of stage-specific gene expression. The 34 kDa polypeptide was a larva-specific antigen and the mRNA was detectable in microfilariae but not in adult worms and eggs. The λ cD34 gene was not sex linked and was contained in the genome of D. immitis at each stage. The stage-specific expression of the developmentally regulated gene in D. immitis may be controlled primarily at the mRNA level.

Abstract 3259: Leukocyte Subset Specific Gene Expression In Acute Stroke Patients

Stroke ◽  
2012 ◽  
Vol 43 (suppl_1) ◽  
Author(s):  
Mateusz G Adamski ◽  
Yan Li ◽  
Hua Yu ◽  
Erin Wagner ◽  
Sareen Amarjeet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acute Stroke ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Fold Increase ◽  
Specific Gene ◽  
Related Gene ◽  
Stroke Patients ◽  
Leukocyte Subset ◽  
Control Subjects

Background: Alterations in gene expression in the peripheral blood of patients with acute stroke have been demonstrated using microarray technology. Whole blood and peripheral blood mononuclear cells (PBMCs) were used in prior studies in which panels of genes diagnostic for stroke were developed. We aimed to determine the cellular sources of alterations in gene expression by studying individual leukocyte subsets. Methods: The expression of four genes previously found to be upregulated in ischemic and hemorrhagic stroke (IL1R2, S100A9, ETS2 and F5) was measured in four leukocyte subsets: CD14+ monocytes, CD4+ T cell lymphocytes, CD20+ B cell lymphocytes and PBMCs. These four genes had been reported in at least two of the previously published stroke-related gene panels. Peripheral blood was obtained from six acute stroke patients (all <48 hours from symptom onset) and 6 age, race and sex matched control subjects. Leukocytes were separated from whole blood using density gradient centrifugation and column magnetic bead cell sorting. The purity of separated leukocyte subsets exceeded 90% and was verified with flow cytometry. Messenger RNA was isolated from each leukocyte subset and analyzed by two step RT PCR and qPCR. The expression of the four stroke-related genes was compared to the expression of a housekeeping gene (GAPDH). The relative expression of individual genes and of the 4 gene panel within cellular subsets was compared between stroke patients and control subjects. Results: Individually, IL1R2 and S100A9 were significantly over-expressed in stroke patients with a 10 fold increase for IL1R2 in PBMCs (p<0.05) and a 3 fold increase for S100A9 in the CD4+ T and CD20+ B lymphocyte subsets (p<0.05). When analyzed as a panel of four genes the expression of IL1R2, S100A9, ETS2 and F5 was significantly higher in both the CD4+ T lymphocytes (p<0.05) and CD20+ B lymphocytes (p<0.05) of stroke patients but not in the monocytes or the PBMCs. Conclusion: These results show the potential diagnostic value of selected genes from panels previously found in microarray studies in stroke patients. They also emphasize the value of panel analysis over that of single gene expression and the potential cellular specificity of alterations in gene expression. Analysis of whole blood and PBMCs alone may not reflect important dynamic changes in stroke-related gene expression.

Reproducible and variable rearrangements of a Tetrahymena thermophila surface protein gene family occur during macronuclear development

1988 ◽  
Vol 8 (11) ◽  
pp. 5043-5046
Author(s):  
J P Kile ◽  
H D Love ◽  
C A Hubach ◽  
G A Bannon
Keyword(s):  
Environmental Regulation ◽  
Cdna Clone ◽  
Multigene Family ◽  
Protein Gene ◽  
Tetrahymena Thermophila ◽  
Surface Protein ◽  
Surface Proteins ◽  
Hybridization Probe ◽  
Macronuclear Development ◽  
Surface Protein Gene

The expression of Tetrahymena surface proteins serotype H3 (SerH3) and serotype T (SerT) is under environmental regulation. SerH3 is expressed when cells are incubated between the temperatures of 20 and 35 degrees C, while SerT is expressed when cells are grown at temperatures above 35 degrees C. Using a SerH3 cDNA clone as a hybridization probe, we determined that (i) the SerH3 gene is a member of a multigene family; (ii) most members of this multigene family are variably rearranged during macronuclear development; and (iii) the gene which produces the SerH3 mRNA is reproducibly rearranged during macronuclear development.

scholarly journals Identification and sequence analysis ofTreponema pallidum tprJ, a member of a polymorphic multigene family

1998 ◽  
Vol 169 (1) ◽  
pp. 155-163 ◽  
Author(s):  
Lola V. Stamm ◽  
Shermalyn R. Greene ◽  
Heather L. Bergen ◽  
John M. Hardham ◽  
Natalie Y. Barnes
Keyword(s):  
Sequence Analysis ◽  
Multigene Family

scholarly journals Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease

2009 ◽  
Vol 106 (13) ◽  
pp. 5099-5104 ◽  
Author(s):  
A. McConnell Smith ◽  
R. Takeuchi ◽  
S. Pellenz ◽  
L. Davis ◽  
N. Maizels ◽  
...  
Keyword(s):  
Gene Conversion ◽  
Homing Endonuclease ◽  
Specific Gene ◽  
Site Specific ◽  
Nicking Enzyme

Isolation and sequence analysis of a cDNA and a related gene for cytochrome P450 proteins from Solanum chacoense

Gene ◽  
1997 ◽  
Vol 188 (2) ◽  
pp. 247-252 ◽  
Author(s):  
György Hutvágner ◽  
Endre Barta ◽  
Zsófia Bánfalvi
Keyword(s):  
Cytochrome P450 ◽  
Sequence Analysis ◽  
Solanum Chacoense ◽  
Related Gene
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