A cadmium-tolerant strain of Neocosmospora vasinfecta shows reduced cadmium influx

1991 ◽  
Vol 69 (6) ◽  
pp. 1296-1301 ◽  
Author(s):  
K. Budd

Neocosmospora vasinfecta was grown in a defined liquid medium and its growth responses to CdSO4 were measured. The specific growth rate of mycelium not previously exposed to cadmium was reduced to 50% by 51 μM cadmium. The growth response to concentrations of cadmium above 50 μM was complex, showing declining and then recovery phases. Inoculum taken from a culture grown to the recovery phase in presence of 100 μM cadmium was cadmium tolerant; a cadmium level of 420 μM was required to reduce its specific growth rate by 50%. Tolerance was not lost by growth in the absence of cadmium. The tolerant mycelium showed markedly reduced net uptake of cadmium as compared with the normal (cadmium sensitive) mycelium. This reduction was due to a decrease in the unidirectional influx of cadmium, with little or no change in efflux. Cadmium toxicity in the normal mycelium was strongly antagonized by calcium but only weakly by manganese or zinc. Calcium also inhibited the uptake of cadmium. Key words: cadmium, calcium, Neocosmospora, tolerance, toxicity, transport.

2017 ◽  
Vol 16 (1) ◽  
pp. 60
Author(s):  
, Purnamawati ◽  
Daniel Djokosetiyanto ◽  
Kukuh Nirmala ◽  
Enang Harris ◽  
Ridwan Affandi

<p><strong>ABSTRACT </strong></p><p> </p><p>The aim of the research was to analyze survival rate, specific growth rate, albumin, and feed efficiency and physiological (blood glucose, cortisol, dan haemoglobin) responses of snake head fish juvenil that reared at aerated and unaerated of tidal land water have been conducted in the laboratory. Experiments using completely randomized design with aerated and unaerated as a treatment, and each treatment has twelve replications. The snakehead fish juvenil wich length 2.4±0.2 cm and weight of 0.21±0.05 g reared in the aquarium that are size 30×25×35 cm (water volume 25 L) with a stocking density 2 juvenile/L, for 40 days. The fishes were fed with commercial feed with protein content about 40%, feeding two times a day (morning and afternoon) were at satiation. Replacement of water done every two days about 10% of the total water volume in the aquarium. The results showed that unaerated median significantly affected to biometric and physiological response of juvenile of snake head fish. The media un-aerated gives the best results shown by the higher value of survival (92%), specific growth rate (6.73%/ day), feed efficiency (78.22%), protein retention (41.91%), energy retention (30.81%) value of albumin (6.60 g/100 mL) and the haemoglobin (5.58 g/dL), and have the lowest value of cortisol (21.49 ng/L) and blood glucose (43.36 mg/100 mL).</p><p> </p><p>Keywords: acid sulfate water, growth rate, aeration, <em>Channa striata </em></p><p><em> </em></p><p> </p><p><strong>ABSTRAK </strong></p><p> </p><p>Penelitian ini bertujuan untuk menganalisis respons kelangsungan hidup, pertumbuhan spesifik, albumin, dan efisiensi pakan dan fisiologis (kortisol, glukosa darah, dan hemoglobin) juvenil ikan gabus yang dipelihara dengan dan tanpa aerasi pada media air rawa pasang surut. Penelitian menggunakan rancangan acak lengkap dengan aerasi dan tanpa aerasi sebagai perlakuan, dan masing-masing perlakuan memiliki 12 ulangan. Juvenil ikan gabus berukuran panjang 2,4±0,3 cm dan bobot 0,21±0,03 g dipelihara dalam akuarium 30×25×35 cm (volume 25 L) dengan padat tebar 2 ekor/L, selama 40 hari. Ikan diberi pakan berupa pakan komersial dengan kadar protein ±40%, pemberian pakan dua kali sehari (pagi dan sore) <em>at satiation</em>. Penggantian air dan penyiponan dilakukan dua hari sekali sebanyak 10% dari volume total dalam akuarium. Hasil penelitian menunjukkan bahwa perlakuan tanpa aerasi berpengaruh nyata terhadap respons biometrik dan fisiologis juvenil ikan gabus. Media tanpa aerasi memberikan hasil yang lebih baik ditunjukkan oleh kelangsungan hidup (92%), laju pertumbuhan spesifik (6,73%/ hari), efisiensi pakan (78,22%), retensi protein (41,91%), retensi energi (30,81%), kadar albumin (6,60 g/100 mL), dan hemoglobin (5,85 g/dL) yang lebih tinggi, sedangkan kadar kortisol (219 ng/L) dan glukosa darah (43,36 mg/100 mL) yang terendah.</p><p> </p><p>Kata kunci: media sulfat masam, pertumbuhan, aerasi, <em>Channa striata</em></p>


Aquaculture ◽  
2008 ◽  
Vol 274 (1) ◽  
pp. 87-95 ◽  
Author(s):  
S. Millot ◽  
M.-L. Bégout ◽  
J. Person-Le Ruyet ◽  
G. Breuil ◽  
C. Di-Poï ◽  
...  

2010 ◽  
Vol 45 (11) ◽  
pp. 1800-1807 ◽  
Author(s):  
S. Chenikher ◽  
J.S. Guez ◽  
F. Coutte ◽  
M. Pekpe ◽  
P. Jacques ◽  
...  

2012 ◽  
Vol 48 (2) ◽  
pp. 382-386 ◽  
Author(s):  
Juan Aguirre ◽  
Mª Rosa Rodríguez ◽  
Rodrigo González ◽  
Gonzalo García de Fernando

1999 ◽  
Vol 70 (1-3) ◽  
pp. 323-333 ◽  
Author(s):  
Marcel Janssen ◽  
Tjibbe Chris Kuijpers ◽  
Bram Veldhoen ◽  
Michel Brik Ternbach ◽  
Johannes Tramper ◽  
...  

2013 ◽  
Vol 48 (6) ◽  
pp. 645-650 ◽  
Author(s):  
Cleujosí da Silva Nunes ◽  
Gilberto Moraes ◽  
Fernando Fabrizzi ◽  
Araceli Hackbarth ◽  
Gustavo Alberto Arbeláez-Rojas

The objective of this work was to evaluate the effect of sustained swimming and dietary protein levels on growth and hematological responses of juvenile pacu (Piaractus mesopotamicus). A completely randomized design was used in a 3x2 factorial arrangement, with three levels of dietary protein (24, 28, and 32% crude protein), two rearing conditions (sustained swimming or motionless water), and 15 replicates. Fish were subjected to sustained swimming at the velocity of two body lengths per second (2 BL s-1), for 45 days. The level of dietary protein and the swimming conditions affected the performance, growth, and hematological profile of pacu. Swimming conditions influenced nutritional factors, increasing daily weight gain, specific growth rate, number of erythrocytes, mean corpuscular volume, and mean corpuscular hemoglobin. Fish under sustained swimming and fed with 24% crude protein showed better growth performance, with higher specific growth rate (4.11±0.88) and higher daily weight gain (2.19±0.47 g per day). Sustained swimming can increase the productive performance of pacu and simultaneously reduce dietary protein levels.


Author(s):  
Dio Patria Yustysi ◽  
Fajar Basuki ◽  
Titik Susilowati ◽  
Tristiana Yuniarti

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh dari hibridisasi ikan nila Pandu F6 dengan nila Nilasa terhadap karakter reproduksinya dan performa benih yang dihasilkan. Penelitian ini dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini: perlakuan A (Pandu F6 ♀><Pandu F6 ♂), B (Nilasa ♀><Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), dan D (Nilasa ♀><Pandu F6 ♂). Data yang diamati meliputi fekunditas, daya tetas telur, diameter dan bobot telur, panjang dan bobot larva TL, panjang dan bobot larva lepas kuning telur, kelulushidupan, laju pertumbuhan spesifik, konversi pakan dan kualitas air. Hasil penelitian menunjukan nilai terbaik pada fekunditas sebesar 1191,67 ± 239,45 butir/200 g, daya tetas telur sebesar 80,93 ± 3,90%, kelulushidupan sebesar 81,55 ± 7,54%, laju pertumbuhan spesifik sebesar 7,26 ± 0,25, konversi pakan 0,54 ± 0,02 didapatkan pada perlakuan C, akan tetapi untuk diameter dan bobot telur, panjang dan bobot larva Kuning Telur, dan panjang dan bobot larva lepas kuning telur tidak berbeda nyata. Hasil tersebut menunjukan bahwa hibridisasi (perlakuan C) memberikan pengaruh yang nyata (P < 0,05) terhadap fekunditas, daya tetas telur, kelulushidupan, laju pertumbuhan spesifik, dan konversi pakan tetapi tidak berpengaruh terhadap diameter dan bobot telur, panjang dan bobot larva, dan panjang dan bobot larva lepas kuning telur. Kata kunci: Hibridisasi, Ikan Nila, Karakter Reproduksi, Performa Benih  ABSTRACT This research aims to know the influence of hybridization tilapia fish Pandu F6 with nila Nilasa against the character of the reproduction and seed performance. This research was conducted with the experimental method using Random Design complete (RAL) with 4 treatments and three replicates. The treatments in this study: the treatment A (Pandu F6 F6 ♀ >< Pandu ♂), B (Nilasa ♀>< Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), and D (Nilasa ♀ >< Pandu F6 ♂). The observed data covering fecundity, hatching rate, egg size, yolk sack larva length and weight, length and weight of the egg yolk off larvae, survival rate, specific growth rate, feed conversion rate, and water quality. The results showed the best value on the fecundity of 1191.67±239.45 eggs/200 g, Hatching rate 80.93 ± 3.90%, Survival rate of 81.55 ± 7.54%, specific growth rate of 7.26 ± 0.25, conversion fodder 0.54 ± 0.02 obtained at the treatment C, but for the diameter and weight of egg, larval length and weight TL, and the length and weight of the yolk egg off larvae for each treatment do not differ markedly. The results showed that hybridization (treatment C) gives a real influence (P < 0.05) of fecundity,hatching rate, survival rate but not with the egg size, larva weight and length, and the length and weight of larvae off yolk. Water quality on the spawning, hatching eggs and larvae found on the maintenance of a decent range for tilapia fish farming Keywords: hybridization, tilapia fish, character of the reproduction, seed performance 


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