Fates of ethanol produced during flooding of sunflower roots
Hypoxic flooding of sunflower (Helianthus annuus L. cv. Delgren 131) roots caused rapid promotion of ethanol synthesis. Some of the root-synthesized ethanol was retained while a major portion was leaked into the flooding medium. A portion of the root ethanol was transported to shoots via transpirational stream, where some of it was vented out to the air around the leaves. Sunflower roots and shoots have the capacity to utilize ethanol retained by incorporating the carbon from ethanol into lipids, amino and organic acids, and sugars. Removal of ethanol from tissues by leaking, by venting, and by metabolic reutilization may be useful mechanisms that sunflowers use to avoid ethanol accumulation. Shoots of flooded sunflower plants, which are of course important as sources of oxygen and carbohydrates for roots, also act as a sink for root ethanol. More ethanol metabolism may be taking place in shoots than in roots. Prolonged flooding stress caused a rapid and steady increase in ADH activity in roots, but ethanol levels showed a decline after an initial rise. ADH in flooded roots may be useful in the regeneration of NAD, thus ensuring continuation of glycolysis.