Synchronous and high frequency germination of interior spruce somatic embryos following partial drying at high relative humidity

1990 ◽  
Vol 68 (5) ◽  
pp. 1086-1090 ◽  
Author(s):  
D. R. Roberts ◽  
B. C. S. Sutton ◽  
B. S. Flinn
Keyword(s):  
Relative Humidity ◽  
Somatic Embryos ◽  
Low Frequency ◽  
High Relative Humidity ◽  
Zygotic Embryos ◽  
Interior Spruce ◽  
Half Strength ◽  
Abnormal Pattern ◽  
Time Required ◽  
Water Saturated

The germination of mature somatic embryos of interior spruce was limited by the low frequency of root emergence. In addition, development was abnormal, since elongation and greening of the hypocotyl and cotyledons preceded root emergence by 1–2 weeks. Pretreatment of the embryos on water-saturated Kim-paks increased the frequency of root emergence but did not alter the abnormal pattern of germination. Somatic embryos do not survive desiccation at room humidity, but partial drying at high humidity promoted germination up to 90%. Furthermore, this treatment decreased the time required for root emergence such that elongation of the root and hypocotyl–cotyledon was synchronized over a period of 5–6 days. This germination closely resembled that of excised zygotic embryos. Drying over a range of humidities indicated that humidities of 81% and lower were lethal to the embryos, whereas germination was enhanced following treatment at humidities greater than 95% relative to untreated controls. The best germination and root elongation occurred on one-half strength basal media containing 2–3.4% sucrose. Of the plantlets derived from treated embryos, 50% survived transfer to soil compared with only 5% of the untreated controls. Key words: conifers, desiccation, germination, high relative humidity, partial drying, somatic embryogenesis, spruce.

Propagation of interior spruce by somatic embryogenesis

1990 ◽  
Vol 20 (11) ◽  
pp. 1759-1765 ◽  
Author(s):  
F. B. Webster ◽  
D. R. Roberts ◽  
S. M. McInnis ◽  
B. C. S. Sutton
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Relative Humidity ◽  
Somatic Embryos ◽  
Large Scale ◽  
Final Height ◽  
High Relative Humidity ◽  
Mature Embryos ◽  
Interior Spruce ◽  
General Production

To apply somatic embryogenesis to clonal propagation of forest species, the technique must be applicable to a broad range of genotypes and allow efficient regeneration of phenotypically normal plants. Seventy-one lines (genotypes) of embryogenic cultures from six open-pollinated families were obtained by culturing immature embryos of interior spruce. Interior spruce represents a mixture of two closely related species, Piceaglauca (Moench) Voss and Piceaengelmannii Parry, from the interior of British Columbia where they hydridize with one another. The abscisic acid dependent developmental profile (the proportion of rooty embryos, shooty embryos, precociously germinating embryos, and mature embryos over a range of abscisic acid concentrations) differed among genotypes, but in general, production of mature somatic embryos was highest at 40 and 60 μM abscisic acid. Treatment of mature embryos with a high relative humidity treatment resulted in partial drying of the embryos and upon rehydration, markedly enhanced germination of the eight genotypes tested. Within 1 week of being placed under germination conditions, somatic embryos treated with the high relative humidity treatment showed 80–100% germination for 12 of the genotypes, and most genotypes had germination rates of greater than 40%. Survival of "emblings" (germinants from somatic embryos) following transfer to soil, acclimatization, and first season's growth in the nursery was 80% or greater for most genotypes. Over 1200 emblings were tested for nursery performance, representing the first large-scale evaluation of conifer somatic embryos under exvitro conditions. Growth rates, final height, shoot and root morphology, and frost hardiness were similar for emblings and seedlings following the first growing season. These results indicate that somatic embryogenesis can be used for the production of planting stock for a range of interior spruce genotypes.

Improvement of plant recovery from avocado zygotic embryos by desiccation under high relative humidity conditions

2017 ◽  
Vol 222 ◽  
pp. 169-174
Author(s):  
Belén Márquez-Martín ◽  
Fernando Pliego-Alfaro ◽  
Carolina Sánchez-Romero
Keyword(s):  
Relative Humidity ◽  
High Relative Humidity ◽  
Zygotic Embryos ◽  
Plant Recovery

Somatic embryogenesis in mature zygotic embryos of Picea likiangensis

Biologia ◽  
2010 ◽  
Vol 65 (5) ◽  
Author(s):  
Shaoyu Chen ◽  
Shanna Chen ◽  
Fang Chen ◽  
Tao Wu ◽  
Yinbin Wang ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Clonal Propagation ◽  
Zygotic Embryos ◽  
Dichlorophenoxyacetic Acid ◽  
Embryogenic Cultures ◽  
Peg 4000 ◽  
Half Strength ◽  
Basal Media ◽  
2,4 Dichlorophenoxyacetic Acid

AbstractSomatic embryogenesis (SE) was successfully induced from mature zygotic embryos of seven families of Picea likiangensis (Franch.) Pritz after 20 weeks culture on initiation medium. Three basal media (one-half strength LM medium, one-half strength LP medium and improved LP medium) with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (6-BA) were tested but only one-half strength LM medium supplemented with 2,4-D and 6-BA was successful for the embryogenic cultures (EC) initiation. The initiation frequencies of EC varied greatly from different families when culturing on the same initiation medium. The highest frequency (41.3%) was induced from one of the families on one-half strength LM medium supplemented with 3 mg L−1 2,4-D and 1.5 mg L−1 6-BA and 16.83% on average for seven families. EC were subcultured and proliferated on the same medium as the initiation one every 10 days. 3 lines of EC induced from the same family were applied in maturation experiment. Cotyledonary somatic embryos were observed after EC were transferred to maturation media of one-half strength LM medium containing 20-80 mg L−1 abscisic acid and 7.5% polyethylene glycol (PEG-4000). However, one-half strength LM medium supplemented with 40 mg L−1 or 60 mg L−1 ABA and 7.5% PEG gave the best maturation and the 3 lines showed different ability in maturation. Over 80% cotyledonary somatic embryos germinated normally on DCR medium containing 0.2% activated carbon. The success on SE induction of the species has provided an effective clonal propagation method for this important tree’s genetic improvement.

scholarly journals Somatic Embryogenesis in Papaya (Carica papaya L. cv. TNAU Papaya CO.8)

2020 ◽  
pp. 18-26
Author(s):  
C. K. Rajesh ◽  
D. Sudhakar ◽  
K. K. Kumar ◽  
C. Kavitha ◽  
G. Karthikeyan ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Callus Induction ◽  
Somatic Embryos ◽  
Carica Papaya ◽  
Naphthalene Acetic Acid ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Half Strength ◽  
Cotyledonary Stage

An efficient indirect somatic embryogenesis protocol for Carica papaya var TNAU Papaya CO.8 was developed using immature zygotic embryos as an explant. Two growth regulators namely 2,4-D and picloram each at 1, 2, 3 mg/L were tested for callus induction and the highest callus induction frequency (83.33%) was observed in MS medium supplemented with 3 mg/L 2,4-D. However the rate of conversion into somatic embryos was highest (63.33%) on MS medium supplemented with 2 mg/L 2,4-D. Maturation of somatic embryos was studied by using MS medium with different concentrations of abscisic acid (ABA) and benzyl amino purine (BAP) along with glutamine (400 mg/L). The maturation of globular embryos was observed to be higher in the combination of ABA (1.5 mg/L), BAP (0.4 mg/L) along with glutamine (400 mg/L). Even though regeneration was observed from cotyledonary stage embryos in presence of different growth regulators like BAP,       α-naphthalene acetic acid (NAA), phloridzin dehydrate kinetin and gibberellic acid, further growth was not observed due to abnormal regenerative structures. Regeneration of cotyledonary stage somatic embryos were highest (77.4%) in half strength MS medium without growth regulators. The well-developed plantlets with shoots and roots were subsequently transferred for hardening.

scholarly journals Effect of 2, 4-D and Picloram on Somatic Embryogenesis in Carica papaya var. P-7-9

2019 ◽  
Vol 29 (1) ◽  
pp. 25-32
Author(s):  
Kulbhushan Chaudhary ◽  
Jai Prakash
Keyword(s):  
Somatic Embryogenesis ◽  
High Frequency ◽  
Somatic Embryos ◽  
Carica Papaya ◽  
Zygotic Embryos ◽  
Shoot Induction ◽  
Free Medium ◽  
Embryo Germination ◽  
Shoot Bud ◽  
Half Strength

Immature zygotic embryos of Carica papaya L. var. P-7-9 were inoculated on half-strength MS with different concentrations of 2,4-D and picloram alone and their combinations. The highest induction (80%) of somatic embryogenesis was MS supplemented with 2,4-D (7.0 mg/l) followed by 2,4-D (4.0 mg/l) + picloram (1.0 mg/l) but no embryogenesis was observed in auxin-free medium. The addition of osmoticum such as PEG and ABA significantly increased the maturation of somatic embryos. BAP, TDZ and NAA were used for shoot induction. Combination of 2.0 mg/l BAP and 0.5 mg/l NAA was found significantly better for shoot bud proliferation and 70% multiplication. The percentage of germinating embryos was enhanced significantly when the MS fortified with GA3 (0.5 mg/l). However, the high frequency of embryo germination and plantlets formation were obtained with 2,4-D (4.0 mg/l) + picloram (1.0 mg/l). Plant Tissue Cult. & Biotech. 29(1): 25-32, 2019 (June)

Embryons somatiques de Vitis rupestris et embryons zygotiques de Vitis sp. : morphologie, histologie, histochimie et développement

1990 ◽  
Vol 68 (11) ◽  
pp. 2305-2315 ◽  
Author(s):  
Olivier Faure
Keyword(s):  
Somatic Embryos ◽  
Vascular System ◽  
Root Apex ◽  
Zygotic Embryos ◽  
Vitis Rupestris ◽  
Saturated Atmosphere ◽  
Vitis Sp ◽  
Water Saturated ◽  
Root Apices

The problem of the development of grapevine somatic embryos into plantlets was examined through a morphological, histological, and histochemical comparison of zygotic and somatic embryos. Only 3% of somatic embryos were capable of developing into plantlets. However, 27% of these embryos had shoot and root apices showing a histological pattern similar to that observed in zygotic embryos; other embryos had root apex but no shoot apex. In comparison with zygotic embryos, somatic embryos showed the following characteristics: acquisition of giant, and often teratologic, organs, retention of a high proliferative capacity among superficial cells, starch and tannin accumulation, important suberization and slight lignification of superficial cells, differentiation of tracheids in the vascular system, and preservation of a high embryogenic potential in the absence of exogenous growth regulators. The water-saturated atmosphere to which grapevine somatic embryos were submitted during in vitro culture could be unfavourable to germination. Under these conditions, embryos built impermeable suberized superficial layers. Key words: somatic embryos, zygotic embryos, Vitis sp., histochemistry, development.

scholarly journals In Vitro Flowering of Plantlets Regenerated from Zygotic Embryo-derived Somatic Embryos of Ginseng

HortScience ◽  
1990 ◽  
Vol 25 (12) ◽  
pp. 1652-1654 ◽  
Author(s):  
Haeng S. Lee ◽  
Jang R. Liu ◽  
Seung G. Yang ◽  
Young H. Lee ◽  
Kwang-W. Lee
Keyword(s):  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Ginseng Root ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Root Explants ◽  
Half Strength ◽  
Dichlorophenoxy Acetic Acid ◽  
Somatic Embryo Induction

Mature zygotic embryos dissected from ginseng (Panax ginseng C.A. Meyer) seeds were cultured on Murashige and Skoog (MS) medium containing various concentrations of 2,4-D and kinetin. Somatic embryos were induced directly from cotyledonary tissue and from intervening callus. The frequency of somatic embryo induction was up to 55% of zygotic embryo explants. Upon transfer onto half-strength MS medium supplemented with 1 mg BA/liter and 1 mg GA3/liter, most somatic embryos developed into plantlets. More than 50% of the plantlets flowered after 4 weeks of culture, and some developed immature fruits in vitro. These results indicate that adulthood of ginseng root explants is not a prerequisite for flowering of plantlets regenerated through somatic embryogenesis. Chemical names used: (2,4 -dichlorophenoxy) acetic acid (2,4-D); N-(2-furanylmethyl) -1H-purin-6-amine(kinetin); N-(phenylmethyl) -1H-purin-6-amine (BA); gibberellic acid (GA3).

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