Lipid synthesis by isolated duckweed (Lemna minor) chloroplasts in the presence of a sublethal concentration of atrazine

1989 ◽  
Vol 67 (8) ◽  
pp. 2261-2265 ◽  
Author(s):  
Gilles Grenier ◽  
Luce Proteau ◽  
Gaston Beaumont
Keyword(s):  
Linolenic Acid ◽  
Lemna Minor ◽  
Specific Radioactivity ◽  
Lipid Synthesis ◽  
Thylakoid Membranes ◽  
Sublethal Concentration ◽  
In Vivo Studies ◽  
High Level ◽  
Sublethal Concentrations

We have examined the effects of a sublethal concentration of atrazine on the incorporation of sodium-[U-14C] acetate into isolated chloroplast lipids of Lemna minor. A high level of [14C]-acetate was incorporated into diacylgalactosylglycerol (DGG) and diacylglycerol (DAG). Consequently, the conversion of phosphatide acid to DAG and that of DAG to DGG seems to be as active in L. minor (an "18:3-plant") as in "16:3-plants." Chloroplast lipids from atrazine-treated plants were more extensively labelled with [14C]-acetate than the control plants. These results are in agreement with our previous in vivo studies which suggest that sublethal concentrations of atrazine stimulate the lipid metabolism of L. minor to form more thylakoid membranes. Furthermore, the specific radioactivity of linolenic acid was the highest in DAG and the lowest in DGG. These data suggest that desaturation of linoleic acid to linolenic acid mainly occurs before the galactosylation of DAG to form DGG in L. minor.

scholarly journals HOXB9 Promotes Laryngeal Squamous Cell Carcinoma Progression by Upregulating MMP12

2021 ◽  
Author(s):  
Chuanhui Sun ◽  
Peng Wang ◽  
Yujiang Chen ◽  
Qiuying Li ◽  
Hua Deng ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Molecular Mechanisms ◽  
Gene Knockout ◽  
Histological Grade ◽  
Cell Cancer ◽  
In Vivo Studies ◽  
Migration And Invasion ◽  
High Level

Abstract Background：The HOX family transcription factor HOXB9 is a crucial element in the progression of various cancers. In the previous study conducted by the investigators, a drastically higher HOXB9 expression was reported in laryngeal squamous cell cancer (LSCC), when compared to adjacent normal laryngeal squamous tissues. Furthermore, a high level of HOXB9 was closely correlated with histological grade and overall survival in LSCC patients. However, the underlying molecular mechanisms have not been fully elucidated.Results: The present study explored the molecular mechanisms of HOXB9 in LSCC progression. Furthermore, the in vitro and in vivo studies revealed that the gene knockout of HOXB9 using the CRISPR/CAS9 system inhibited cell proliferation, migration and invasion, and promoted cell apoptosis. Mechanistic studies in LSCC cell lines and human LSCC specimens demonstrated that HOXB9 promotes LSCC progression by directly upregulating the MMP12 expression at the level of its transcription.Conclusions: Collectively, the present study is the first to demonstrate the role of HOXB9 in the regulation of LSCC progression by enhancing the upregulation of MMP12.

scholarly journals Secretion and storage of newly synthesized hepatic triacylglycerol fatty acids in vivo in different nutritional states and in diabetes

1988 ◽  
Vol 255 (3) ◽  
pp. 929-935 ◽  
Author(s):  
J M Duerden ◽  
G F Gibbons
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Diurnal Variation ◽  
Low Density Lipoprotein ◽  
Specific Radioactivity ◽  
Lipid Synthesis ◽  
Density Lipoprotein ◽  
Dark Phase ◽  
Light Phase

Hepatic lipid synthesis was measured in rats in vivo with 3H2O, and the appearance of label in triacylglycerol and its constituent fatty acid and glycerol moieties was determined. In rats treated with Triton WR1339, the amount of newly synthesized fatty acid secreted as very-low-density lipoprotein (VLDL) triacylglycerol was greater during the dark phase of the diurnal cycle than during the light phase (11.3 versus 4.8 mumol of 3H2O/3 h per g of liver respectively). However, the total mass of VLDL triacylglycerol secreted remained constant, as did the amount of label in the secreted triacylglycerol glycerol. Newly synthesized fatty acids comprised only a small proportion of the total VLDL triacylglycerol fatty acids (TGFA) at both times (dark phase, 7.7%; light phase, 2.4%). Starvation for 24 h resulted in a small increase in the secretion of VLDL triacylglycerol. However, the contribution from newly synthesized fatty acids was decreased. Similar effects were observed in streptozotocin-diabetic animals. During the light and dark phases of the cycle, similar quantities of newly synthesized TGFA entered the hepatic cytosol, and these amounts were much smaller than those secreted as VLDL triacylglycerol. The mass of cytosolic triacylglycerol showed a diurnal variation, with a greater concentration during the light phase than in the dark. In diabetes, the mass of triacylglycerol was increased in the cytosol, as was the incorporation of labelled acylglycerol glycerol. Diabetes also abolished the diurnal variation in the quantity of cytosolic triacylglycerol. In each group of animals the specific radioactivity of the microsomal triacylglycerol was similar to that of the respective newly secreted plasma VLDL. The specific radioactivity of the cytosolic triacylglycerol was only 15.8% (dark phase) or 16.8% (light phase) that of the microsomal triacylglycerol. This increased to 35.5% in the starved animals and 40.2% in the diabetic animals.

Bioactive anti-oxidative polycaprolactone/gelatin electrospun nanofibers containing selenium nanoparticles/vitamin E for wound dressing applications

2021 ◽  
pp. 088532822110013
Author(s):  
Mohsen Doostmohammadi ◽  
Hamid Forootanfar ◽  
Mojtaba Shakibaie ◽  
Masoud Torkzadeh-Mahani ◽  
Hamid-Reza Rahimi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Vitamin E ◽  
Electrospun Nanofibers ◽  
Inflammatory Cells ◽  
Selenium Nanoparticles ◽  
In Vivo Studies ◽  
Solution Ph ◽  
High Level ◽  
Biogenic Selenium Nanoparticles

In this study, polycaprolactone/gelatin (PCL/GEL) electrospun nanofibers containing biogenic selenium nanoparticles (Se NPs) and Se NPs/vitamin E (VE) with average diameters of 397.8 nm and 279.5 nm, respectively (as determined by SEM inspection) were prepared and their effect on wound healing was evaluated using in-vivo studies. The energy dispersive X-ray (EDX) mapping, TEM micrograph, and FTIR spectra of the prepared nanofibers strongly demonstrated well entrapment of Se NPs and VE into scaffolds. An amount of 57% Se NPs and 43% VE were gradually released from PCL/GEL/Se NPs/VE scaffold after 4 days immersion in PBS solution (pH 7.4). The both PCL/GEL/Se NPs and PCL/GEL/Se NPs/VE scaffolds supported 3T3 cell proliferation and attachment as confirmed by MTT assay and SEM imaging. Complete re-epithelialization, low level of edema and inflammatory cells in coordination with high level of oriented collagens demonstrated the wound healing activity of PCL/GEL/Se NPs/VE. Besides, significant antioxidant efficacy of PCL/GEL/Se NPs and PCL/GEL/Se NPs/VE scaffolds was demonstrated according to GSH and MDA assays. To sum up, the prepared PCL/GEL/Se NPs/VE scaffold in the present study represented suitable healing effect on animal model which candidate it for further studies.

scholarly journals Reversal of renal dysfunction by targeted administration of VEGF into the stenotic kidney: a novel potential therapeutic approach

2012 ◽  
Vol 302 (10) ◽  
pp. F1342-F1350 ◽  
Author(s):  
Alejandro R. Chade ◽  
Silvia Kelsen
Keyword(s):  
Renal Dysfunction ◽  
Renal Injury ◽  
Renal Scarring ◽  
In Vivo Studies ◽  
Large Animal Model ◽  
Therapeutic Effects ◽  
Large Animal ◽  
Targeted Intervention ◽  
High Level

Renal microvascular (MV) damage and loss contribute to the progression of renal injury in renovascular disease (RVD). Whether a targeted intervention in renal microcirculation could reverse renal damage is unknown. We hypothesized that intrarenal vascular endothelial growth factor (VEGF) therapy will reverse renal dysfunction and decrease renal injury in experimental RVD. Unilateral renal artery stenosis (RAS) was induced in 14 pigs, as a surrogate of chronic RVD. Six weeks later, renal blood flow (RBF) and glomerular filtration rate (GFR) were quantified in vivo in the stenotic kidney using multidetector computed tomography (CT). Then, intrarenal rhVEGF-165 or vehicle was randomly administered into the stenotic kidneys ( n = 7/group), they were observed for 4 additional wk, in vivo studies were repeated, and then renal MV density was quantified by 3D micro-CT, and expression of angiogenic factors and fibrosis was determined. RBF and GFR, MV density, and renal expression of VEGF and downstream mediators such as p-ERK 1/2, Akt, and eNOS were significantly reduced after 6 and at 10 wk of untreated RAS compared with normal controls. Remarkably, administration of VEGF at 6 wk normalized RBF (from 393.6 ± 50.3 to 607.0 ± 45.33 ml/min, P < 0.05 vs. RAS) and GFR (from 43.4 ± 3.4 to 66.6 ± 10.3 ml/min, P < 0.05 vs. RAS) at 10 wk, accompanied by increased angiogenic signaling, augmented renal MV density, and attenuated renal scarring. This study shows promising therapeutic effects of a targeted renal intervention, using an established clinically relevant large-animal model of chronic RAS. It also implies that disruption of renal MV integrity and function plays a pivotal role in the progression of renal injury in the stenotic kidney. Furthermore, it shows a high level of plasticity of renal microvessels to a single-dose VEGF-targeted intervention after established renal injury, supporting promising renoprotective effects of a novel potential therapeutic intervention to treat chronic RVD.

scholarly journals Digalloyl Glycoside: A Potential Inhibitor of Trypanosomal PFK from Euphorbia abyssinica J.F. Gmel

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 173
Author(s):  
Seham S. El-Hawary ◽  
Rabab Mohammed ◽  
Nadia M. Lithy ◽  
Sameh Fekry AbouZid ◽  
Mostafa A. Mansour ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Binding Free Energy ◽  
Md Simulations ◽  
Tsetse Fly ◽  
In Vivo Studies ◽  
Spectroscopic Techniques ◽  
Phytochemical Investigation ◽  
High Level

Human African trypanosomiasis is an endemic infectious disease caused by Trypanosoma brucei via the bite of tsetse-fly. Most of the drugs used for the treatment, e.g., Suramin, have shown several problems, including the high level of toxicity. Accordingly, the discovery of anti-trypanosomal drugs from natural sources has become an urgent requirement. In our previous study on the anti-trypanosomal potential of Euphorbia species, Euphorbia abyssinica displayed significant anti-trypanosomal activity. Therefore, a phytochemical investigation of the methanolic extract of E. abyssinica was carried out. Twelve compounds, including two triterpenes (1, 2); one sterol-glucoside (4); three ellagic acid derivatives (3, 9, 11); three gallic acid derivatives (5, 6, 10); and three flavonoids (7, 8, 12), were isolated. The structures of isolated compounds were determined through different spectroscopic techniques. Compound (10) was obtained for the first time from genus Euphorbia while all other compounds except compound (4), were firstly reported in E. abyssinica. Consequently, an in silico study was used to estimate the anti-trypanosomal activity of the isolated compounds. Several compounds displayed interesting activity where 1,6-di-O-galloyl-d-glucose (10) appeared as the most potent inhibitor of trypanosomal phosphofructokinase (PFK). Moreover, molecular dynamics (MD) simulations and ADMET calculations were performed for 1,6-di-O-galloyl-d-glucose. In conclusion, 1,6-di-O-galloyl-d-glucose revealed high binding free energy as well as desirable molecular dynamics and pharmacokinetic properties; therefore, it could be suggested for further in vitro and in vivo studies for trypanosomiasis.

Effets physiologiques de l'atrazine à doses sublétales sur Lemna minor L. IV. Influence sur la composition lipidique

1979 ◽  
Vol 57 (9) ◽  
pp. 1015-1020 ◽  
Author(s):  
G. Grenier ◽  
J. P. Marier ◽  
G. Beaumont
Keyword(s):  
Fatty Acids ◽  
Linolenic Acid ◽  
Acid Content ◽  
Neutral Lipids ◽  
Lemna Minor ◽  
Fatty Acid Content ◽  
Linoleic Acid Content ◽  
Gas Liquid Chromatography ◽  
Total Fatty Acids ◽  
Sublethal Concentrations

The lipids and the fatty acids of duck weed (Lemna minor L.), cultivated aseptically during 5, 10, or 15 days in mineral solution containing sublethal concentrations of atrazine (0.05 to 0.75 ppm), were analysed by thin-layer and gas–liquid chromatography. All concentrations of atrazine used, independently of age of plants, increased the total fatty acid content, except for 5-day plants at 0.50 and 0.75 ppm atrazine where a decrease in total fatty acids was observed. α-Linolenic acid content increased while linoleic acid content decreased. Sublethal concentrations of atrazine increased the percentage of monogalactosyldiacylglycerol compared with total phospholipids and total neutral lipids. Monogalactosyldiacylglycerol was the main lipid involved in the α-linolenic acid increase observed in the total fatty acids. The increase in α-linolenic acid and in monogalactosyldiacylglycerol in the presence of sublethal concentrations of atrazine is an indication that these treatments maintain intact and fully functional chloroplast membranes.

scholarly journals A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and Potency

Biomedicines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 42 ◽  
Author(s):  
Hachidai Aizawa ◽  
Hideo Kawabata ◽  
Atsushi Sato ◽  
Hideo Masuki ◽  
Taisuke Watanabe ◽  
...  
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Platelet Rich Plasma ◽  
In Vivo Studies ◽  
Negative Effects ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
High Level ◽  
Activation Status

It is generally accepted that citrate or the A-form of acid-citrate-dextrose (ACD-A) are suitable for preparing platelet-rich plasma (PRP) for regenerative therapy. However, this is based on evidence from blood transfusions and not from regenerative medicine. Thus, we examined the effects of anticoagulants, such as ACD-A, ethylenediaminetetraacetic acid (EDTA), and heparin, on the regenerative quality of PRP to address this gap. The blood samples were collected in the presence of anticoagulants and were processed to prepare pure-PRP. Platelet size, activation status, and intra-platelet free Ca2+ concentration were determined while using a hematology analyzer and flow cytometer. Platelet-derived growth factor-BB (PDGF-BB) was quantified while using an ELISA. In pure-PRP samples, EDTA caused platelet swelling and activation, but yielded the highest number of platelets. Heparin aggregated platelets and disturbed the overall counting of blood cells. However, no significant differences in PDGF-BB levels were observed among the anticoagulants tested. Moreover, when considering the easy preparation of platelet suspensions, without the need for high-level pipetting skills, these findings suggest the comparable potency of EDTA-derived pure-PRP in tissue regeneration and support the use of EDTA in the preparation of pure-PRP. Further in vivo studies are required in animal models to exclude the possible negative effects of including EDTA in pure-PRP preparations.

scholarly journals High in vitro activity of DIS-73285, a novel antimicrobial with a new mechanism of action, against MDR and XDR Neisseria gonorrhoeae

2020 ◽  
Vol 75 (11) ◽  
pp. 3244-3247
Author(s):  
Susanne Jacobsson ◽  
Clive Mason ◽  
Nawaz Khan ◽  
Paul Meo ◽  
Magnus Unemo
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Small Molecule ◽  
Mechanism Of Action ◽  
Vitro Activity ◽  
In Vivo Studies ◽  
In Vitro Activity ◽  
Agar Dilution ◽  
High Level

Abstract Background The rising incidence of antimicrobial resistance in Neisseria gonorrhoeae may result in untreatable gonorrhoea in certain circumstances and development of novel antimicrobials is urgently needed. Objectives To evaluate the in vitro activity of a novel small-molecule antimicrobial with a new mechanism of action, DIS-73285, against a large geographically, temporally and genetically diverse collection of clinical N. gonorrhoeae isolates and reference strains, including various types of high-level resistant, MDR and XDR gonococcal isolates (n = 262). Methods MICs (mg/L) of DIS-73285 were determined by agar dilution and by Etest for ceftriaxone, cefixime, azithromycin, ciprofloxacin, ampicillin, spectinomycin and tetracycline. Results DIS-73285 was substantially more potent than any of the currently or previously used therapeutic antimicrobials, with MICs ranging from ≤0.001 to 0.004 mg/L, and the MIC50, MIC90 and modal MIC all ≤0.001 mg/L (lowest MIC tested). No correlation with the MICs of DIS-73285 and the MICs of any of the currently or previously used antimicrobials was observed. Conclusions The novel chemotype, small-molecule antimicrobial DIS-73285, demonstrated high in vitro potency against all tested N. gonorrhoeae isolates. Further in vitro and in vivo studies, evaluating efficacy, resistance emergence, pharmacokinetic/pharmacodynamic parameters, toxicity and safety, should be conducted to evaluate DIS-73285 as a therapy specifically for urogenital and extra-genital gonorrhoea.

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