Chrysanthemum phloem necrosis: detection by epifluorescence microscopy

1989 ◽  
Vol 67 (4) ◽  
pp. 1024-1031
Author(s):  
R. J. McGovern ◽  
R. K. Horst ◽  
H. W. Israel
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Nucleic Acid ◽  
Acridine Orange ◽  
Ethidium Bromide ◽  
Diagnostic Procedures ◽  
Epifluorescence Microscopy ◽  
Transmission Electron ◽  
Phloem Necrosis ◽  
Mycoplasmalike Organisms

In epifluorescence diagnostic procedures for chrysanthemum phloem necrosis, autofluorescence, aniline blue, and various nucleic acid "specific" fluorochromes such as 33258-Hoechst, 4′,6-diamidino-2-phenyl-indole-dichloride, berberine sulfate, ethidium bromide, and acridine orange were utilized. Increased fluorescence in foliar phloem correlated precisely with gross symptoms of chrysanthemum phloem necrosis by all techniques and was used, in the case of berberine sulfate, to facilitate detection of mycoplasmalike organisms by transmission electron microscopy.

Tumor Diagnosis

1982 ◽  
Vol 40 ◽  
pp. 262-265
Author(s):  
Bruce Mackay
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Diagnosis ◽  
Light Microscopy ◽  
Clinical Examination ◽  
Ultrastructural Study ◽  
Diagnostic Procedures ◽  
Specific Diagnosis ◽  
Transmission Electron ◽  
Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

Egg morphology and hatching in Mormidea pictiventris (Hemiptera: Pentatomidae)

2001 ◽  
Vol 79 (4) ◽  
pp. 726-736 ◽  
Author(s):  
Klaus W Wolf ◽  
Walton Reid
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Amorphous Material ◽  
Follicle Cells ◽  
Epifluorescence Microscopy ◽  
Stink Bug ◽  
Anterior Plate ◽  
Hexagonal Pattern ◽  
Transmission Electron ◽  
Egg Morphology

Egg morphology and hatching in the stink bug Mormidea pictiventris (Hemiptera: Pentatomidae) are described with the aid of scanning electron microscopy. In addition, the eggshell is analyzed using transmission electron microscopy and the distribution of follicle cells at the surface of ovarian eggs is studied by epifluorescence microscopy using a DNA-specific fluorescent dye. The surface of the barrel-shaped eggs carries numerous slender processes. Binucleate follicle cells, in most cases arranged in a hexagonal pattern, are responsible for the synthesis of this portion of the eggshell. The rim at the anterior pole of the egg is studded at irregular intervals with short columnar processes, the aero-micropylar processes. Hatching occurs at this pole. The prolarva is wrapped in an embryonic cuticle. Its head portion carries a Y-shaped element, the egg-burster. The major features of the inner face of the eggshell are subtle, radially oriented grooves at the anterior plate and a hexagonal pattern to the surface ornamentation throughout the remainder of the eggshell. Transmission electron microscopy revealed that the processes extending from the surface of the eggshell have a coarse texture, while the eggshell proper is composed of amorphous material. The innermost layer, however, has a trabecular organization. The findings in M. pictiventris are compared with morphological observations on the eggshell in other families of Hemiptera and suggestions are made concerning the meaning of the diverse structures.

scholarly journals Hydrogels of Polycationic Acetohydrazone-Modified Phosphorus Dendrimers for Biomedical Applications: Gelation Studies and Nucleic Acid Loading

Pharmaceutics ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 120 ◽  
Author(s):  
Evgeny K. Apartsin ◽  
Alina E. Grigoryeva ◽  
Audrey Malrin-Fournol ◽  
Elena I. Ryabchikova ◽  
Alya G. Venyaminova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Polyethylene Glycol ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Biomedical Applications ◽  
Structural Units ◽  
Transmission Electron ◽  
Phosphate Buffered Saline ◽  
Acid Loading

In this work, we report the assemblage of hydrogels from phosphorus dendrimers in the presence of biocompatible additives and the study of their interactions with nucleic acids. As precursors for hydrogels, phosphorus dendrimers of generations 1–3 based on the cyclotriphosphazene core and bearing ammonium or pyridinium acetohydrazones (Girard reagents) on the periphery have been synthesized. The gelation was done by the incubation of dendrimer solutions in water or phosphate-buffered saline in the presence of biocompatible additives (glucose, glycine or polyethylene glycol) to form physical gels. Physical properties of gels have been shown to depend on the gelation conditions. Transmission electron microscopy revealed structural units and well-developed network structures of the hydrogels. The hydrogels were shown to bind nucleic acids efficiently. In summary, hydrogels of phosphorus dendrimers represent a useful tool for biomedical applications.

scholarly journals Pneumonia in a Captive Central Bearded Dragon With Concurrent Detection of Helodermatid Adenovirus 2 and a Novel Mycoplasma Species

2018 ◽  
Vol 55 (6) ◽  
pp. 900-904 ◽  
Author(s):  
Nicholas A. Crossland ◽  
Peter M. DiGeronimo ◽  
Yulia Sokolova ◽  
April L. Childress ◽  
James F. X. Wellehan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Nucleic Acid ◽  
Etiologic Agent ◽  
Mycoplasma Species ◽  
Transmission Electron ◽  
Bearded Dragon ◽  
Lymphocytic Interstitial Pneumonia ◽  
Pogona Vitticeps ◽  
Pcr Targeting

A 4-year-old captive male central bearded dragon ( Pogona vitticeps) was presented for recurrent episodic dyspnea and anorexia with occasional expulsion of oral mucoid discharge. Despite empirical antimicrobial therapy and supportive care, the animal died and was submitted for autopsy. Defining histologic features included heterophilic and lymphocytic interstitial pneumonia, with occasional amphophilic intranuclear inclusions and prominent type II pneumocyte hyperplasia. Transmission electron microscopy revealed intranuclear 80-nm, nonenveloped, hexagonal viral particles within pneumocytes. Helodermatid adenovirus 2 (HeAdV2) was determined as the etiologic agent through pan-adenoviral consensus polymerase (PCR) chain reaction and sequencing. Nucleic acid from a novel Mycoplasma sp. (provisionally called Mycoplasma pogonae) was identified by pan-generic PCR targeting the mycoplasma 16S ribosomal RNA gene with sequencing and phylogenetic analysis. As bacteria morphologically consistent with Mycoplasma sp. were not observed by special stains and transmission electron microscopy, the detection of M. pogonae nucleic acid is of indeterminate significance; however, M. pogonae and HeAdV2 coinfection may have exacerbated disease.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

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