The developmental patterns of endo-β-1,4-glucanase and β-glucosidase of Chaetomium erraticum

1988 ◽  
Vol 66 (11) ◽  
pp. 2162-2166 ◽  
Author(s):  
D. K. Sandhu ◽  
R. Puri
Keyword(s):  
Carbon Source ◽  
Metal Ions ◽  
Carboxymethyl Cellulose ◽  
Sole Carbon Source ◽  
Ethylenediaminetetraacetic Acid ◽  
Molecular Forms ◽  
Relative Distribution ◽  
Developmental Patterns ◽  
Multiple Molecular Forms ◽  
Response To Temperature

The present investigation deals with the production of extracellular and intracellular endoglucanase and β-glucosidase enzymes and the study of their multiple molecular forms in Chaetomium erraticum. When C. erraticum was grown on carboxymethyl cellulose as the sole carbon source, more than 90% of the endoglucanase was extracellular, while most of the β-glucosidase was intracellular. However, the relative distribution of these enzymes varied with the age of the culture. Two forms of each of endoglucanase (EG-I, EG-II) and β-glucosidase (βGlu-I, βGlu-II) were detected after 4 and 6 days of incubation, respectively. Additional intracellular forms of endoglucanase and β-glucosidase i.e., EG-III and βGlu-III, appeared at later stages, when perithecia developed, indicating that EG-III and βGlu-III are related to the development of perithecia. These multiple molecular forms were further differentiated by their response to temperature, pH, ethylenediaminetetraacetic acid, and metal ions.

Acidobacteria from oligotrophic soil from the Cerrado can grow in a wide range of carbon source concentrations

2013 ◽  
Vol 59 (11) ◽  
pp. 746-753 ◽  
Author(s):  
Virgilio Hipólito Lemos de Castro ◽  
Luis Felipe Schroeder ◽  
Betania Ferraz Quirino ◽  
Ricardo Henrique Kruger ◽  
Cristine Chaves Barreto
Keyword(s):  
Carbon Source ◽  
16S Rrna ◽  
Growth Rates ◽  
Carboxymethyl Cellulose ◽  
Sole Carbon Source ◽  
Solid Medium ◽  
Colloidal Chitin ◽  
Brazilian Cerrado ◽  
Liquid Media ◽  
Wide Range

Soils from the Brazilian Cerrado are nutrient-poor, acidic, and aluminum-rich. A previous study revealed that members of the phylum Acidobacteria were predominant in these oligotrophic soils. Five acidobacteria from Cerrado soil were isolated on VL-55 medium containing 0.05% of xylan as carbon source. All isolates belong to the Acidobacteria subdivision 1, and their 16S rRNA showed similarities of 94.2%–96% with Acidobacterium capsulatum or 98.6% with Edaphobacter aggregans. All isolates were able to sustain growth in a wide range of carbon source concentrations. Growth occurred in all concentrations of arabinose, dextrose, and xylose; only one isolate did not grow on fructose. Isolates grew poorly on N-acetyl-d-glucosamine at all concentrations tested. In general, increasing concentrations of these monosaccharides did not inhibit growth rates. Isolates exhibited growth on solid medium containing xylan, carboxymethyl cellulose, and colloidal chitin; however, growth was observed on solid medium that did not contain these polysaccharides. These isolates may be able to use the solidifying agents tested (gellan gum or agar) as carbon source. This interpretation is supported by the absence of growth in liquid media containing chitin or carboxymethyl cellulose at 0.05% as sole carbon source, whereas growth in the same conditions using xylan was confirmed.

scholarly journals Production of laccases, cellulases and xylanases of Pleurotus ostreatus grown in liquid-state fermentation

2017 ◽  
Vol 2 (2) ◽  
pp. 169-176
Author(s):  
Edna María Hernández-Domínguez ◽  
Carmen Sánchez ◽  
Gerardo Díaz-Godínez
Keyword(s):  
Carbon Source ◽  
Kinetic Parameters ◽  
Liquid State ◽  
Pleurotus Ostreatus ◽  
Carboxymethyl Cellulose ◽  
Sole Carbon Source ◽  
Culture Medium ◽  
Carbon Sources ◽  
The Third

In this study, activities of laccases, xylanases and cellulases produced by Pleurotus ostreatus in liquid-state fermentation were evaluated. Three fermentations were done by triplicate where the carbon source was changed, one was made with glucose, in another was used carboxymethylcellulose and xylan and in the third the three carbon sources were added, in all cases, copper was added as inducer of laccases. The kinetic parameters of growth of the fungus were obtained. It was observed that this fungus produced the three enzymes evaluated; laccases showed the highest values (34,240 U/L) in the culture medium with glucose as sole carbon source. Cellulases showed their highest activity in the culture medium with xylan and carboxymethylcellulose (12,858 U/L) and xylanases in medium with glucose, carboxymethyl cellulose and xylan (27,153 U/L). Up to 4 isoform of laccases, 2 of xylanase and 2 of cellulases were observed by zymography.

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp

scholarly journals Study of a plugging microbial consortium using crude oil as sole carbon source

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Microbial Consortium

Multiple Molecular Forms of Chorismate Mutase in Bacillus subtillis*

Biochemistry ◽  
1967 ◽  
Vol 6 (5) ◽  
pp. 1541-1553 ◽  
Author(s):  
Janet H. Lorence ◽  
Eugene W. Nester
Keyword(s):  
Chorismate Mutase ◽  
Molecular Forms ◽  
Multiple Molecular Forms
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